##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049526_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8247866 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.8783845421349 31.0 31.0 34.0 28.0 34.0 2 31.109186545950188 31.0 31.0 34.0 28.0 34.0 3 31.168035222686694 31.0 31.0 34.0 28.0 34.0 4 34.77396735106996 37.0 35.0 37.0 32.0 37.0 5 34.796158666981256 37.0 35.0 37.0 32.0 37.0 6 34.768676164234485 37.0 35.0 37.0 32.0 37.0 7 30.83986912493486 37.0 33.0 37.0 2.0 37.0 8 32.58829726380133 37.0 33.0 37.0 17.0 37.0 9 35.38452309967209 39.0 34.0 39.0 29.0 39.0 10-11 35.95538397447291 39.0 36.0 39.0 31.0 39.0 12-13 36.13190314925097 39.0 37.0 39.0 32.0 39.0 14-15 37.324818262081365 40.0 38.0 41.0 31.5 41.0 16-17 37.33329772816387 40.0 38.0 41.0 31.5 41.0 18-19 37.32249214524096 40.0 37.5 41.0 31.0 41.0 20-21 37.397458324371414 40.0 38.0 41.0 31.0 41.0 22-23 37.3497153081779 40.0 37.5 41.0 31.0 41.0 24-25 37.32184809986001 40.0 37.0 41.0 31.0 41.0 26-27 37.34829694129367 40.0 37.0 41.0 31.0 41.0 28-29 37.32978803244379 40.0 37.0 41.0 31.0 41.0 30-31 37.25346131957042 40.0 37.0 41.0 31.0 41.0 32-33 37.2123678415726 40.0 37.0 41.0 31.0 41.0 34-35 37.15777499294969 40.0 37.0 41.0 31.0 41.0 36-37 36.986993484132746 40.0 37.0 41.0 30.0 41.0 38-39 36.876963568515784 40.0 37.0 41.0 30.0 41.0 40-41 36.8094690796383 40.0 37.0 41.0 30.0 41.0 42-43 36.695296456077244 40.0 36.5 41.0 30.0 41.0 44-45 36.57011201442894 40.0 36.0 41.0 30.0 41.0 46-47 36.4178700284413 40.0 36.0 41.0 30.0 41.0 48-49 36.34161860777078 40.0 36.0 41.0 30.0 41.0 50-51 36.200975379570906 39.5 35.5 41.0 29.0 41.0 52-53 36.08440825784512 39.0 35.0 41.0 29.0 41.0 54-55 35.96521997326339 39.0 35.0 41.0 29.0 41.0 56-57 35.7660414827302 39.0 35.0 41.0 28.0 41.0 58-59 35.58798197012415 39.0 35.0 41.0 28.0 41.0 60-61 35.37186497695283 38.5 35.0 40.0 28.0 41.0 62-63 35.13834160254301 38.0 34.0 40.0 28.0 41.0 64-65 34.77891663128378 38.0 34.0 40.0 27.0 41.0 66-67 31.28128118230825 36.0 31.0 40.0 6.5 41.0 68-69 31.64426034564577 36.0 30.5 39.0 11.0 41.0 70-71 31.804306168407685 35.5 30.0 39.0 15.5 41.0 72-73 33.04342486892973 35.0 32.5 39.0 25.0 40.5 74-75 33.125753679800326 35.0 33.0 38.5 26.0 40.0 76-77 32.827820250716975 35.0 33.0 37.5 25.5 39.5 78-79 29.430474137188938 35.0 29.0 37.0 6.5 39.0 80-81 31.22626343589966 35.0 30.0 36.0 21.5 39.0 82-83 31.631246373304318 35.0 32.0 36.0 23.0 37.5 84-85 29.789428198760746 35.0 30.5 35.5 12.0 37.0 86-87 29.419297670451968 34.5 29.0 35.0 13.5 37.0 88-89 30.663302483333265 34.0 30.5 35.0 20.0 36.0 90-91 30.815044946171533 35.0 32.0 35.0 20.0 36.0 92-93 30.670833946623283 35.0 32.0 35.0 18.5 36.0 94-95 30.468376789826607 35.0 32.0 35.0 12.5 36.0 96-97 30.27958365230473 35.0 32.0 35.0 4.5 35.0 98-99 30.094016864483493 35.0 32.0 35.0 2.0 35.0 100 29.89105678971991 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2692043992308193 1101 2 0.273249671765484 1101 3 0.30678233301958713 1101 4 0.2779337560773527 1101 5 0.26707379485629446 1101 6 0.24189356398526485 1101 7 -12.202560207904302 1101 8 -6.602821298543933 1101 9 -2.3630115168659174 1101 10-11 -0.25816312476790415 1101 12-13 0.3002105139881692 1101 14-15 0.4715648176229905 1101 16-17 0.5493310130315052 1101 18-19 0.4798197280411429 1101 20-21 0.46718661731498656 1101 22-23 0.47655852572079027 1101 24-25 0.5162946046093992 1101 26-27 0.48027999811551325 1101 28-29 0.3932815458092236 1101 30-31 0.4860339668437277 1101 32-33 0.5213829233925367 1101 34-35 0.4953125456639711 1101 36-37 0.5610534884553573 1101 38-39 0.54899658996694 1101 40-41 0.5869989213300713 1101 42-43 0.5574471618215924 1101 44-45 0.485643215589036 1101 46-47 0.4657230400672674 1101 48-49 0.39950050692734607 1101 50-51 0.5225835627651634 1101 52-53 0.5180656557855414 1101 54-55 0.43141380993337464 1101 56-57 0.43954813887846456 1101 58-59 0.4420360661586784 1101 60-61 0.4079292898086493 1101 62-63 0.42151541983048446 1101 64-65 0.3120565504278048 1101 66-67 -10.299789832139162 1101 68-69 -8.218093863450594 1101 70-71 -6.740538392278783 1101 72-73 -1.5534971962097401 1101 74-75 -0.25797471413716266 1101 76-77 0.04768449450613588 1101 78-79 3.220716411254166 1101 80-81 1.1027222704260602 1101 82-83 0.4112602497328659 1101 84-85 -5.401302708671761 1101 86-87 -5.888653687369381 1101 88-89 -1.338323921857782 1101 90-91 -0.037943208624927394 1101 92-93 0.31464836218326653 1101 94-95 0.3817013908574687 1101 96-97 0.47170328747685986 1101 98-99 0.49690295776274596 1101 100 0.5458294720751766 1102 1 0.2091764494447439 1102 2 0.2617206527965479 1102 3 0.25465221512562053 1102 4 0.26005685439040604 1102 5 0.27058590613272315 1102 6 0.18493743957943565 1102 7 -12.252315995398806 1102 8 -6.654941062020477 1102 9 -2.377413797408501 1102 10-11 -0.2805782962031387 1102 12-13 0.21728932663641132 1102 14-15 0.4162924687733778 1102 16-17 0.48123744069678764 1102 18-19 0.4958797865186142 1102 20-21 0.4952180560041626 1102 22-23 0.408973033937734 1102 24-25 0.49971104553191736 1102 26-27 0.4283407451617407 1102 28-29 0.4696819048932852 1102 30-31 0.4206885327669099 1102 32-33 0.4540009271537002 1102 34-35 0.4779542128394354 1102 36-37 0.5398684173472859 1102 38-39 0.5874585421245371 1102 40-41 0.5089789215942915 1102 42-43 0.47248271764136973 1102 44-45 0.4325947902995608 1102 46-47 0.4897264764841722 1102 48-49 0.3507105143289664 1102 50-51 0.41963831567484533 1102 52-53 0.44681226783801264 1102 54-55 0.3930404058972172 1102 56-57 0.48222944919071864 1102 58-59 0.40531450411982917 1102 60-61 0.2977368431419407 1102 62-63 0.2988028534595628 1102 64-65 0.18093133231557346 1102 66-67 -10.324805903801725 1102 68-69 -6.71686668646111 1102 70-71 -6.289957571944708 1102 72-73 -1.1386152829847518 1102 74-75 -0.1763647218387021 1102 76-77 -0.024781208271377864 1102 78-79 3.165200220337276 1102 80-81 1.0325864900939585 1102 82-83 0.38891724680185646 1102 84-85 -5.500369423075863 1102 86-87 -5.960357492474966 1102 88-89 -1.3667394112450388 1102 90-91 -0.12312592124664334 1102 92-93 0.2359415789330015 1102 94-95 0.2619987242022255 1102 96-97 0.35470513216987953 1102 98-99 0.37661256634077134 1102 100 0.3793518181576552 1103 1 0.22931949758567072 1103 2 0.26551784403332235 1103 3 0.23585569760898295 1103 4 0.20682915159588333 1103 5 0.2516807357219548 1103 6 0.2956486526909501 1103 7 -12.278707509043212 1103 8 -6.63709650268456 1103 9 -2.319397628009675 1103 10-11 -0.274504014644954 1103 12-13 0.28125502891010257 1103 14-15 0.47200278190683775 1103 16-17 0.4314405450825234 1103 18-19 0.4657862846749694 1103 20-21 0.45933617410311456 1103 22-23 0.4516210672611507 1103 24-25 0.5456843050222915 1103 26-27 0.4349340085321387 1103 28-29 0.49511412177445635 1103 30-31 0.43073948859183275 1103 32-33 0.5575782222594583 1103 34-35 0.5294807145292779 1103 36-37 0.5688454547529389 1103 38-39 0.5700422304099035 1103 40-41 0.557214756678917 1103 42-43 0.5497684922528947 1103 44-45 0.4695994861549053 1103 46-47 0.49463408225029326 1103 48-49 0.46293018697432586 1103 50-51 0.4300036104590248 1103 52-53 0.391943841366988 1103 54-55 0.3944715016826663 1103 56-57 0.43887279324292905 1103 58-59 0.4101707073207592 1103 60-61 0.3107432479304393 1103 62-63 0.2496671197304039 1103 64-65 0.1438739957573958 1103 66-67 -10.450264343210605 1103 68-69 -8.377770605768092 1103 70-71 -6.889642169558783 1103 72-73 -1.655871030268031 1103 74-75 -0.42918239821057114 1103 76-77 -0.11102504581132422 1103 78-79 3.0767435962186553 1103 80-81 0.9458702543165138 1103 82-83 0.27004599197687185 1103 84-85 -5.57947433591357 1103 86-87 -6.047212219180526 1103 88-89 -1.452786139439997 1103 90-91 -0.14016852115142697 1103 92-93 0.22171496604612173 1103 94-95 0.2737521120500013 1103 96-97 0.43501744939116804 1103 98-99 0.4707976886941836 1103 100 0.5237561132520412 1104 1 0.1076162594607517 1104 2 0.112382040059952 1104 3 0.11938683894358704 1104 4 0.1289495035124517 1104 5 0.12452615703428904 1104 6 0.17451329352900302 1104 7 -12.23445824326813 1104 8 -6.67894665366039 1104 9 -2.437635908555883 1104 10-11 -0.3823070064362426 1104 12-13 0.1730112589940873 1104 14-15 0.3688612472574704 1104 16-17 0.349072892514279 1104 18-19 0.31915133021628606 1104 20-21 0.34105427096079666 1104 22-23 0.35185208749366836 1104 24-25 0.4293988300641587 1104 26-27 0.3750671165661075 1104 28-29 0.34100130101839454 1104 30-31 0.3506036449703558 1104 32-33 0.32368084586441626 1104 34-35 0.30258777248822355 1104 36-37 0.3839053433437769 1104 38-39 0.42934465338482397 1104 40-41 0.4472737284377075 1104 42-43 0.3681628788068778 1104 44-45 0.3781100100967052 1104 46-47 0.3406862359194136 1104 48-49 0.2769012090762786 1104 50-51 0.3576544054304236 1104 52-53 0.32293408771977994 1104 54-55 0.32836348258762627 1104 56-57 0.31587971198521814 1104 58-59 0.29788810279932676 1104 60-61 0.27977139840701426 1104 62-63 0.2086385927940384 1104 64-65 0.09655312293216411 1104 66-67 -10.464440337898324 1104 68-69 -8.474344274474497 1104 70-71 -6.942101663828478 1104 72-73 -1.8928045165217782 1104 74-75 -0.6418298904765152 1104 76-77 -0.36906658308751616 1104 78-79 2.8799621620564366 1104 80-81 0.7504819586613358 1104 82-83 0.06150529161735463 1104 84-85 -5.670995666740428 1104 86-87 -6.134176771876096 1104 88-89 -1.61659218400359 1104 90-91 -0.3042076980684776 1104 92-93 0.03607386985383343 1104 94-95 0.06854338105813085 1104 96-97 0.15483961517270828 1104 98-99 0.17502202812143963 1104 100 0.2362550133475949 1105 1 0.11185524151027693 1105 2 0.13710745429049354 1105 3 0.13859187362318792 1105 4 0.15968060698544662 1105 5 0.1339253496554349 1105 6 0.18761528598755461 1105 7 -12.242151814085492 1105 8 -6.694409558375483 1105 9 -2.5234638899172666 1105 10-11 -0.3942814657983078 1105 12-13 0.14402120155893527 1105 14-15 0.33873106592580626 1105 16-17 0.3663384942005621 1105 18-19 0.32050101966140687 1105 20-21 0.3274653846254978 1105 22-23 0.3557375085014769 1105 24-25 0.4033985468951826 1105 26-27 0.34850234420907356 1105 28-29 0.3274886209384462 1105 30-31 0.397846357329648 1105 32-33 0.41401381018290806 1105 34-35 0.37332978931081584 1105 36-37 0.4770807097064633 1105 38-39 0.39123316821711285 1105 40-41 0.4011989773176694 1105 42-43 0.2970225931845647 1105 44-45 0.25064068450354426 1105 46-47 0.23750778816761198 1105 48-49 0.23273282480334245 1105 50-51 0.3199114070413742 1105 52-53 0.32148579514569775 1105 54-55 0.27883452965105704 1105 56-57 0.22970403999715217 1105 58-59 0.21188080754360783 1105 60-61 0.17710894525415455 1105 62-63 0.12200092741080937 1105 64-65 0.02934922674928231 1105 66-67 -10.379890569131497 1105 68-69 -8.443291971656201 1105 70-71 -6.868488422050689 1105 72-73 -1.7856169622514457 1105 74-75 -0.5617549290798678 1105 76-77 -0.22456839393692718 1105 78-79 2.949509541150938 1105 80-81 0.8430814720353474 1105 82-83 0.13114708352201987 1105 84-85 -5.560774460572215 1105 86-87 -6.044866367711418 1105 88-89 -1.5381577406811608 1105 90-91 -0.2603319978065137 1105 92-93 0.10622278293573828 1105 94-95 0.16118704987504273 1105 96-97 0.24344780194438087 1105 98-99 0.26084024309656684 1105 100 0.3923899559000432 1106 1 0.14345957500898265 1106 2 0.1632538039592646 1106 3 0.15824527333080596 1106 4 0.17345658502403438 1106 5 0.15516330374193643 1106 6 0.1509709918895581 1106 7 -12.275412390461923 1106 8 -6.739480237840542 1106 9 -2.559500622540803 1106 10-11 -0.4223496575136281 1106 12-13 0.16368104095007396 1106 14-15 0.3248923242817625 1106 16-17 0.3529043434368049 1106 18-19 0.33992183523269404 1106 20-21 0.3475285192441717 1106 22-23 0.32668849157880686 1106 24-25 0.4347279930344996 1106 26-27 0.4032884292071657 1106 28-29 0.3816789050024383 1106 30-31 0.37187223945618086 1106 32-33 0.3706565334225189 1106 34-35 0.3877651034935852 1106 36-37 0.44143289152942344 1106 38-39 0.41016579597803826 1106 40-41 0.424441682366691 1106 42-43 0.4208832988754949 1106 44-45 0.3329948746204252 1106 46-47 0.39898666229231594 1106 48-49 0.4132179538444163 1106 50-51 0.43331635578544336 1106 52-53 0.4151888038217848 1106 54-55 0.3857767078195167 1106 56-57 0.3634665998314759 1106 58-59 0.3048046627387535 1106 60-61 0.32717844593241097 1106 62-63 0.24242112528044402 1106 64-65 0.10465908789163336 1106 66-67 -10.489193715963385 1106 68-69 -8.388390027010022 1106 70-71 -6.8743971837271935 1106 72-73 -1.7105260824990438 1106 74-75 -0.47417976956589314 1106 76-77 -0.1927995414740593 1106 78-79 3.018676924793688 1106 80-81 0.8896084748372068 1106 82-83 0.2169887561062005 1106 84-85 -5.59966220473564 1106 86-87 -6.04093911883842 1106 88-89 -1.458260551852181 1106 90-91 -0.1480788811427587 1106 92-93 0.14284595114236254 1106 94-95 0.1851016457183512 1106 96-97 0.29721558104091983 1106 98-99 0.2900850964857433 1106 100 0.3700977905771943 1107 1 0.08223600733742131 1107 2 0.11411979012617479 1107 3 0.11152333068737263 1107 4 0.05791547083765636 1107 5 0.09533759363606009 1107 6 0.06623024761294971 1107 7 -12.292158780212635 1107 8 -6.746371407540007 1107 9 -2.634140960553985 1107 10-11 -0.5372852194284619 1107 12-13 0.11989723030917077 1107 14-15 0.2626111264779354 1107 16-17 0.247734933356206 1107 18-19 0.25726691017055003 1107 20-21 0.20303618498095943 1107 22-23 0.2665990402914673 1107 24-25 0.310275864197493 1107 26-27 0.25401088931976545 1107 28-29 0.2588989087232889 1107 30-31 0.26984219488328876 1107 32-33 0.3103340383298132 1107 34-35 0.2735871810028385 1107 36-37 0.34410085170689086 1107 38-39 0.3307202851736051 1107 40-41 0.33817066126522377 1107 42-43 0.3383393638287657 1107 44-45 0.31053501016934604 1107 46-47 0.3596916859087216 1107 48-49 0.28387198303074257 1107 50-51 0.31412352575478053 1107 52-53 0.30681394485996805 1107 54-55 0.24373124778312416 1107 56-57 0.265244513795416 1107 58-59 0.25513071239247154 1107 60-61 0.2679016848424638 1107 62-63 0.2670115410115841 1107 64-65 0.09397256344244909 1107 66-67 -10.420307896791325 1107 68-69 -8.377124928566214 1107 70-71 -6.865129338187764 1107 72-73 -1.7700339078235388 1107 74-75 -0.4696946762298069 1107 76-77 -0.20515204778310903 1107 78-79 2.9934799186853596 1107 80-81 0.8765852774657183 1107 82-83 0.23248208064736886 1107 84-85 -5.598923768707195 1107 86-87 -6.11105482197814 1107 88-89 -1.5426752686088605 1107 90-91 -0.3229775634350531 1107 92-93 0.051217690799362 1107 94-95 0.14115422277009415 1107 96-97 0.16440997510087385 1107 98-99 0.2362973922316769 1107 100 0.32487866327937454 1108 1 0.06960589551076879 1108 2 0.1188073993090768 1108 3 0.12593375211768887 1108 4 0.05209901974951947 1108 5 0.04012875643110192 1108 6 0.08311658400212707 1108 7 -12.358240847155919 1108 8 -6.781357154762578 1108 9 -2.6382845805310495 1108 10-11 -0.5152800042250973 1108 12-13 0.07442429292638764 1108 14-15 0.25181016133451095 1108 16-17 0.24853408336649352 1108 18-19 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-0.8427579242187733 2216 7 3.243207525745582 2216 8 1.3653348728579573 2216 9 0.15327698893314334 2216 10-11 -0.592705936220078 2216 12-13 -0.9402526303879526 2216 14-15 -1.0922012237501662 2216 16-17 -1.269667428869056 2216 18-19 -1.7345861541265535 2216 20-21 -1.2939399994870229 2216 22-23 -1.166021778697342 2216 24-25 -1.1828318396789541 2216 26-27 -1.601138570651024 2216 28-29 -1.739569023143524 2216 30-31 -1.619799561594327 2216 32-33 -2.620084599544242 2216 34-35 -1.6706774156847501 2216 36-37 -1.3420159195353136 2216 38-39 -1.5463877115650106 2216 40-41 -1.6019757451777679 2216 42-43 -1.2375715408483998 2216 44-45 -1.3110354175004488 2216 46-47 -1.505319140888595 2216 48-49 -1.5830828027880486 2216 50-51 -2.108180070262705 2216 52-53 -1.7970260708159245 2216 54-55 -1.4039893448757184 2216 56-57 -1.4737611863056514 2216 58-59 -1.799165395552194 2216 60-61 -1.8658372033859578 2216 62-63 -1.7772695911980634 2216 64-65 -1.4993675497286887 2216 66-67 1.9285328354694826 2216 68-69 1.1216662878137527 2216 70-71 0.7723083978035952 2216 72-73 -1.025954399818282 2216 74-75 -1.837277081270841 2216 76-77 -1.4799858045312035 2216 78-79 1.133891216868257 2216 80-81 -0.367923368743849 2216 82-83 -1.273425193538209 2216 84-85 0.5665284593414732 2216 86-87 0.4947063787334649 2216 88-89 -0.7717790073821078 2216 90-91 -1.0700216763203763 2216 92-93 -1.1642808415019559 2216 94-95 -1.1376820919192348 2216 96-97 -1.0936032254609778 2216 98-99 -1.109604537681392 2216 100 -2.300676168162674 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 111718.0 3 21734.0 4 12916.0 5 11371.0 6 10379.0 7 11399.0 8 12759.0 9 14482.0 10 16707.0 11 19222.0 12 21198.0 13 24205.0 14 27973.0 15 29815.0 16 32324.0 17 33301.0 18 31809.0 19 29984.0 20 28849.0 21 29330.0 22 31042.0 23 36161.0 24 43552.0 25 54551.0 26 68888.0 27 85527.0 28 106189.0 29 134914.0 30 167575.0 31 206605.0 32 274098.0 33 386383.0 34 586566.0 35 807327.0 36 896217.0 37 1514628.0 38 1738292.0 39 576640.0 40 1236.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94831344204119 20.39567458074036 14.742087442134807 22.91392453508364 2 15.261293827383227 20.210740755963897 40.755672905884076 23.7722925107688 3 18.459925152847894 25.103743545809266 30.336315078720936 26.100016222621903 4 12.848254833834808 17.019201483662833 38.6380026615918 31.494541020910567 5 13.300963975732438 37.62303706310036 35.461711954749184 13.614287006418019 6 30.72277017158374 37.706337834000486 17.93160075552427 13.639291238891508 7 27.243273941953067 32.557578961888076 22.284834562377405 17.91431253378146 8 24.857281247935074 36.36473196396886 20.550991074659926 18.226995713436146 9 24.622405359155934 17.57146636475423 21.85057322706261 35.955555049027225 10-11 23.708981207987616 27.29974032070841 28.8652786066117 20.12599986469227 12-13 24.15518147360784 24.680183940912716 28.99896167081279 22.165672914666654 14-15 21.598326160973034 27.049857502534596 26.12822516757668 25.223591168915693 16-17 21.21969454895166 30.314226077860063 26.885532573970465 21.580546799217807 18-19 21.13622481257576 29.34536036351706 28.721865752911118 20.79654907099606 20-21 22.19767782329124 28.495046900015943 28.313951549885942 20.993323726806874 22-23 21.933711212919267 28.292586730469843 28.391909397649613 21.38179265896128 24-25 21.720090990809016 28.41841150183575 28.328473086250433 21.533024421104805 26-27 21.456596920796795 28.447899379329005 28.362919636734336 21.732584063139864 28-29 21.501173758157567 28.48782945794706 28.50606447776916 21.504932306126214 30-31 21.832448649896147 28.767261056815247 28.05140311757024 21.348887175718374 32-33 21.511043435672768 28.59703000661929 28.396766023104043 21.4951605346039 34-35 21.682389525336497 28.358718087495138 28.378383785183654 21.580508601984707 36-37 21.89518666835276 28.495890390031942 28.057407112606015 21.551515829009286 38-39 21.712154010336253 28.637863970080975 28.203060380981153 21.446921638601616 40-41 21.665459990830353 28.450997412603297 28.280989790631285 21.602552805935062 42-43 21.587535144715996 28.42105192964261 28.277839049552423 21.713573876088972 44-45 21.734494969748166 28.492761293173764 28.186863299788218 21.585880437289852 46-47 21.70505167243014 28.556906903420664 28.125420757492915 21.61262066665628 48-49 21.628732115538178 28.4313626830518 28.16751056827019 21.772394633139836 50-51 21.644991071593118 28.436866803300553 28.107210476644045 21.810931648462287 52-53 21.640969029467108 28.450315476536886 28.10618295214262 21.80253254185338 54-55 21.690685008787465 28.60800876254883 28.016271191865833 21.685035036797867 56-57 21.633499750901066 28.44918969796163 28.047739698464802 21.869570852672503 58-59 21.551552985481834 28.245366559327444 28.26260137579059 21.940479079400127 60-61 21.7539779434672 28.469073513955696 27.981213809494104 21.795734733083 62-63 21.56785254284944 28.54770148661614 27.992016399324076 21.892429571210346 64-65 21.622909602386002 28.330659682316668 28.1182326429207 21.928198072376627 66-67 21.537929349511316 28.335653748761587 28.04937945268653 22.077037449040567 68-69 21.97395749392614 28.459319258153474 27.874530909609362 21.692192338311024 70-71 21.728924044420904 28.274382976824725 28.018690520222027 21.978002458532348 72-73 21.58742783633587 28.38917789044157 28.179926755737476 21.84346751748508 74-75 21.570645390311267 28.461936852951176 28.053860094048073 21.913557662689485 76-77 21.627027948208056 28.383893749072808 27.941799933299745 22.04727836941939 78-79 21.77191548129384 28.39114609257341 28.0440280023969 21.792910423735854 80-81 21.5999648859822 28.323638481544254 28.13570694878646 21.940689683687083 82-83 21.5661543139911 28.33534850312412 28.22273785672264 21.875759326162143 84-85 21.690222374279173 28.217173957260428 28.0599764712105 22.032627197249898 86-87 21.748221953374184 28.307140049377182 28.019793552836518 21.924844444412113 88-89 21.605719895934655 28.19931974995461 28.124644408548928 22.07031594556181 90-91 21.585364278916185 28.15545952328166 28.113144052230144 22.14603214557201 92-93 21.692096151515624 28.2676100422847 28.019099743069013 22.02119406313066 94-95 21.627919045273742 28.077451350558967 28.110855517701538 22.183774086465746 96-97 21.59674813505788 28.114791317447857 28.148716440893235 22.139744106601032 98-99 21.654633515836288 28.079378176397164 28.119057564516137 22.14693074325041 100 21.733952446168647 28.031091444032796 28.002415340321086 22.23254076947747 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1537.0 1 1229.0 2 1085.0 3 1247.0 4 1228.5 5 1167.0 6 1071.5 7 1044.0 8 1101.0 9 1149.5 10 1105.0 11 1062.5 12 1108.5 13 1179.0 14 1307.5 15 1538.5 16 1880.0 17 2358.5 18 3182.0 19 4410.0 20 6351.0 21 9456.0 22 14438.5 23 21891.0 24 31192.5 25 42770.0 26 58277.0 27 77906.0 28 102700.0 29 128961.0 30 152996.0 31 176608.5 32 198267.5 33 218109.0 34 237801.0 35 256366.5 36 271104.0 37 283567.0 38 294879.0 39 301217.5 40 305272.5 41 305064.0 42 299480.5 43 295248.0 44 288588.5 45 280688.0 46 275699.0 47 268149.5 48 259372.0 49 254512.5 50 248703.5 51 238632.0 52 226245.5 53 215321.0 54 206827.0 55 196114.5 56 179997.0 57 161401.5 58 144150.5 59 127932.5 60 110715.0 61 92368.0 62 76547.0 63 62853.0 64 50831.5 65 40409.5 66 31273.0 67 24072.5 68 18193.5 69 13570.0 70 9988.0 71 7188.0 72 5195.5 73 3652.0 74 2537.0 75 1730.5 76 1136.5 77 751.0 78 538.0 79 412.0 80 285.5 81 202.5 82 146.0 83 106.0 84 96.0 85 77.0 86 63.0 87 58.5 88 48.5 89 38.0 90 29.5 91 23.5 92 18.5 93 20.5 94 22.0 95 21.0 96 19.5 97 15.5 98 19.0 99 33.0 100 50.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.018719993753535762 2 0.002388496612335845 3 0.001503419187460126 4 0.002061139208614689 5 0.0017944035463233763 6 0.0042192732035171284 7 11.574678832076078 8 1.2124348285968758E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.6979262272204713E-4 18-19 0.0 20-21 1.2124348285968758E-5 22-23 6.062174142984379E-6 24-25 0.0 26-27 2.1823826914743767E-4 28-29 0.0 30-31 6.062174142984379E-6 32-33 3.03108707149219E-5 34-35 2.4248696571937516E-5 36-37 3.6373044857906275E-5 38-39 4.849739314387503E-5 40-41 6.062174142984379E-6 42-43 5.455956728685942E-5 44-45 2.2430044329042203E-4 46-47 0.0011215022164521103 48-49 7.759582903020005E-4 50-51 4.1222784172293775E-4 52-53 3.1523305543518774E-4 54-55 4.546630607238284E-4 56-57 0.0016852844117496574 58-59 3.212952295781721E-4 60-61 0.001279118744169704 62-63 0.0025339887917674706 64-65 0.0011275643905950944 66-67 5.787515219088186 68-69 5.437321605370407 70-71 0.0031826414250667994 72-73 0.0014670461426022198 74-75 0.003340257952784393 76-77 0.006322847631132707 78-79 5.378427583571314 80-81 0.0054256458579710195 82-83 0.005116474976678816 84-85 5.787988068671339 86-87 0.004025283630941628 88-89 0.001982330944755892 90-91 0.0035342475253598927 92-93 0.003885853625652987 94-95 0.004752744528099754 96-97 0.0035281853512169085 98-99 0.006195541974130035 100 0.007044246354147849 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8247866.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.02727835423991 #Duplication Level Percentage of deduplicated Percentage of total 1 90.65607279994931 77.98895209263478 2 6.619347785557304 11.388889489433197 3 1.4487420580451322 3.7389400887282895 4 0.5300904610427413 1.8240895858020512 5 0.2670309863112644 1.1485974494303184 6 0.14372025634626812 0.7418317498705872 7 0.0892861876673979 0.5376733403756501 8 0.06516392669829192 0.4484700208583396 9 0.04057268442921589 0.31413218552738015 >10 0.13834818590513068 1.7629887558234203 >50 0.0014450712776270162 0.08360240867994248 >100 1.7959677030065228E-4 0.02183283283605197 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.2124348285968758E-5 0.0 4 0.0 0.0 0.0 7.274608971581255E-5 0.0 5 0.0 2.4248696571937516E-5 0.0 7.274608971581255E-5 0.0 6 0.0 2.4248696571937516E-5 0.0 7.274608971581255E-5 0.0 7 0.0 2.4248696571937516E-5 0.0 9.699478628775007E-5 0.0 8 1.2124348285968758E-5 2.4248696571937516E-5 0.0 9.699478628775007E-5 0.0 9 4.849739314387503E-5 2.4248696571937516E-5 0.0 1.3336783114565635E-4 0.0 10-11 6.062174142984379E-5 3.6373044857906275E-5 0.0 1.454921794316251E-4 0.0 12-13 6.062174142984379E-5 3.6373044857906275E-5 0.0 1.5155435357460948E-4 0.0 14-15 6.062174142984379E-5 6.668391557282816E-5 0.0 1.8792739843251577E-4 0.0 16-17 6.062174142984379E-5 9.699478628775007E-5 0.0 2.1823826914743765E-4 0.0 18-19 7.274608971581255E-5 9.699478628775007E-5 0.0 2.2430044329042203E-4 0.0 20-21 8.487043800178131E-5 9.699478628775007E-5 0.0 2.606734881483283E-4 0.0 22-23 8.487043800178131E-5 9.699478628775007E-5 0.0 3.5766827443607836E-4 0.0 24-25 8.487043800178131E-5 9.699478628775007E-5 0.0 5.213469762966566E-4 0.0 26-27 9.093261214476568E-5 9.699478628775007E-5 0.0 9.214504697336256E-4 0.0 28-29 9.699478628775007E-5 1.2124348285968758E-4 0.0 0.0030795844646360647 0.0 30-31 9.699478628775007E-5 1.2124348285968758E-4 0.0 0.009256939916337147 0.0 32-33 9.699478628775007E-5 1.2124348285968758E-4 0.0 0.022605847379188748 0.0 34-35 1.0911913457371882E-4 1.2124348285968758E-4 0.0 0.044702472130366815 0.0 36-37 1.0911913457371882E-4 1.2124348285968758E-4 0.0 0.0787415799432241 0.0 38-39 1.0911913457371882E-4 1.2124348285968758E-4 0.0 0.1253900099734889 0.0 40-41 1.0911913457371882E-4 1.2124348285968758E-4 0.0 0.17391771398807887 0.0 42-43 1.0911913457371882E-4 1.2124348285968758E-4 0.0 0.22373666109512447 0.0 44-45 1.151813087167032E-4 1.3943000528864074E-4 0.0 0.2869408887098796 0.0 46-47 1.2124348285968758E-4 1.5155435357460948E-4 0.0 0.36294842811461775 0.0 48-49 1.2730565700267197E-4 1.5761652771759387E-4 0.0 0.44370871204745566 0.0 50-51 1.3336783114565635E-4 1.5761652771759387E-4 0.0 0.5251843325291657 0.0 52-53 1.5761652771759387E-4 1.6974087600356261E-4 0.0 0.6063871551744415 0.0 54-55 1.5761652771759387E-4 1.6974087600356261E-4 0.0 0.6957072289971733 0.0 56-57 1.6974087600356264E-4 1.75803050146547E-4 0.0 0.7925201500606338 0.0 58-59 1.8186522428953139E-4 1.8186522428953139E-4 0.0 0.8994084045497345 0.0 60-61 1.8186522428953139E-4 1.8186522428953139E-4 0.0 1.0107159839890707 0.0 62-63 2.061139208614689E-4 1.8186522428953139E-4 0.0 1.1197684346472165 0.0 64-65 2.061139208614689E-4 1.8186522428953139E-4 0.0 1.2386234208945686 0.0 66-67 2.1823826914743765E-4 1.8186522428953139E-4 0.0 1.3705605789424804 0.0 68-69 2.1823826914743765E-4 1.8186522428953139E-4 0.0 1.5036737987741313 0.0 70-71 2.1823826914743765E-4 1.8186522428953139E-4 0.0 1.66028400558399 0.0 72-73 2.2430044329042203E-4 1.9398957257550013E-4 0.0 1.826696747983054 0.0 74-75 2.4248696571937516E-4 1.9398957257550013E-4 0.0 1.978796697230532 0.0 76-77 2.4248696571937516E-4 1.9398957257550013E-4 0.0 2.137934830658015 0.0 78-79 2.5461131400534394E-4 1.9398957257550013E-4 0.0 2.3290327461673117 0.0 80-81 2.5461131400534394E-4 2.061139208614689E-4 0.0 2.5559035512943593 0.0 82-83 2.5461131400534394E-4 2.1217609500445326E-4 0.0 2.7922131615620334 6.062174142984379E-6 84-85 2.5461131400534394E-4 2.1823826914743765E-4 0.0 3.029535154911586 1.2124348285968758E-5 86-87 2.5461131400534394E-4 2.1823826914743765E-4 0.0 3.3085103467975836 1.2124348285968758E-5 88 2.5461131400534394E-4 2.1823826914743765E-4 0.0 3.542431460452922 1.2124348285968758E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8490 0.0 44.60644 1 GTATCAA 13010 0.0 35.91427 1 TCAACGC 13755 0.0 32.660816 4 ATCAACG 13890 0.0 32.417053 3 CAACGCA 15295 0.0 29.472677 5 TATCAAC 16080 0.0 28.479353 2 AACGCAG 16830 0.0 26.81425 6 GTACATG 23795 0.0 23.658134 1 ACGCAGA 19215 0.0 23.139536 7 CGCAGAG 19640 0.0 22.506536 8 TACATGG 24125 0.0 22.227318 2 ACATGGG 24955 0.0 20.79091 3 GTCGTAC 460 0.0 18.913183 1 GCAGAGT 22725 0.0 18.615013 9 GTGGTAT 4885 0.0 18.01928 1 CATGGGG 17325 0.0 17.661236 4 CAGAGTA 21480 0.0 16.68204 10-11 AGAGTAC 19560 0.0 16.388124 10-11 GAGTACA 18110 0.0 16.333637 1 TGGTATC 5640 0.0 14.969179 2 >>END_MODULE