FastQCFastQC Report
Wed 25 May 2016
SRR2049526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049526_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8247866
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204700.24818540941378048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200670.24329929705453507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114630.1389814044020599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149000.056.2507741
GTATCAA263850.041.1168821
ATCAACG324800.032.9166373
TCAACGC328000.032.681584
TATCAAC337950.031.8586032
CAACGCA340800.031.4014115
GTGGTAT61550.030.3708671
AACGCAG360350.029.8017776
ACGCAGA409100.026.1349587
CGCAGAG409250.025.9531258
TGGTATC70200.025.4723872
GCAGAGT444100.023.2392189
GTACATG240700.021.772781
GAGTACT295000.021.50709212-13
CAGAGTA430350.020.65093210-11
TACATGG246500.020.5790352
AGAGTAC406800.020.21170410-11
ACATGGG252700.019.440993
GTACTTT321750.019.39766514-15
AGTACTT311050.019.08284412-13
Produced by FastQC (version 0.11.5)