##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049526_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8247866
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70756314421209	31.0	31.0	34.0	30.0	34.0
2	31.852081132258938	31.0	31.0	34.0	30.0	34.0
3	31.978279472532652	33.0	31.0	34.0	30.0	34.0
4	35.65239857194576	37.0	35.0	37.0	33.0	37.0
5	35.72221226678513	37.0	35.0	37.0	33.0	37.0
6	35.825669694439746	37.0	35.0	37.0	35.0	37.0
7	35.82201250602277	37.0	35.0	37.0	35.0	37.0
8	35.82171655068111	37.0	35.0	37.0	35.0	37.0
9	37.56108513402133	39.0	37.0	39.0	35.0	39.0
10-11	37.51362200840799	39.0	37.0	39.0	35.0	39.0
12-13	37.494129996777346	39.0	37.0	39.0	35.0	39.0
14-15	38.783510304362366	40.0	38.0	41.0	35.0	41.0
16-17	38.79157420840737	40.0	38.0	41.0	35.0	41.0
18-19	38.85796089558196	40.0	38.0	41.0	35.0	41.0
20-21	38.88795131977169	40.0	38.0	41.0	35.0	41.0
22-23	38.86813152638513	40.0	38.0	41.0	35.0	41.0
24-25	38.8319701726483	40.0	38.0	41.0	35.0	41.0
26-27	38.74799002554116	40.0	38.0	41.0	35.0	41.0
28-29	38.668866952979116	40.0	38.0	41.0	34.0	41.0
30-31	38.571976678573584	40.0	38.0	41.0	34.0	41.0
32-33	38.44873098083795	40.0	38.0	41.0	34.0	41.0
34-35	38.35722646318454	40.0	38.0	41.0	34.0	41.0
36-37	38.233692872798855	40.0	38.0	41.0	34.0	41.0
38-39	38.15309384269822	40.0	38.0	41.0	34.0	41.0
40-41	38.04480183116457	40.0	38.0	41.0	33.0	41.0
42-43	37.913787820995154	40.0	37.5	41.0	33.0	41.0
44-45	37.74500131306692	40.0	37.0	41.0	33.0	41.0
46-47	37.52297951979337	40.0	37.0	41.0	33.0	41.0
48-49	37.31570796615755	40.0	37.0	41.0	32.0	41.0
50-51	37.1140644137526	40.0	37.0	41.0	31.5	41.0
52-53	37.01340346703014	40.0	36.5	41.0	31.0	41.0
54-55	36.887943378323556	40.0	36.0	41.0	31.0	41.0
56-57	36.71956370290206	39.0	36.0	41.0	31.0	41.0
58-59	36.55144664086444	39.0	35.0	41.0	31.0	41.0
60-61	36.36953837513849	39.0	35.0	41.0	31.0	41.0
62-63	36.174507078558264	39.0	35.0	41.0	31.0	41.0
64-65	35.90161182298549	38.5	35.0	40.5	30.0	41.0
66-67	35.647833451707385	38.0	35.0	40.0	30.0	41.0
68-69	35.343704601893386	37.0	35.0	40.0	29.5	41.0
70-71	35.052381185630324	37.0	34.5	39.5	29.0	41.0
72-73	34.73251481049765	36.0	34.0	39.0	29.0	41.0
74-75	34.3960657217273	36.0	34.0	39.0	29.0	41.0
76-77	34.09933066322853	35.0	34.0	38.5	29.0	40.0
78-79	33.77293005245235	35.0	34.0	37.0	29.0	39.0
80-81	33.46075318148961	35.0	34.0	37.0	29.0	39.0
82-83	33.163326610786356	35.0	34.0	36.5	28.0	39.0
84-85	32.8871677837637	35.0	34.0	36.0	28.0	37.0
86-87	32.61194719943316	35.0	33.0	36.0	27.0	37.0
88-89	32.40627763593638	35.0	33.0	35.0	27.0	37.0
90-91	32.25708540851658	35.0	33.0	35.0	27.0	36.0
92-93	32.10498230693855	35.0	33.0	35.0	27.0	36.0
94-95	31.928238043634558	35.0	33.0	35.0	26.5	36.0
96-97	31.768331469012715	35.0	33.0	35.0	26.0	36.0
98-99	31.59401001422671	35.0	33.0	35.0	25.5	35.0
100	31.485007273396537	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3541754436611697
1101	2	0.3782742111303534
1101	3	0.47396110198591046
1101	4	0.38637332087271403
1101	5	0.2955012459803257
1101	6	0.2657399724210805
1101	7	0.2894361347294705
1101	8	0.2979461333655209
1101	9	0.30305548233118174
1101	10-11	0.32483985499068524
1101	12-13	0.3037352171379055
1101	14-15	0.4517761192907699
1101	16-17	0.48737033750050784
1101	18-19	0.45005554342200327
1101	20-21	0.37690902760138556
1101	22-23	0.44052232708740746
1101	24-25	0.4833647934274623
1101	26-27	0.4695618734101288
1101	28-29	0.4328837689709317
1101	30-31	0.46152599473590783
1101	32-33	0.46198496535186706
1101	34-35	0.519253120630367
1101	36-37	0.5130199107118258
1101	38-39	0.510962730144449
1101	40-41	0.495521917171331
1101	42-43	0.48523737206071615
1101	44-45	0.46057049893182267
1101	46-47	0.49769750466834495
1101	48-49	0.48936965616168493
1101	50-51	0.5620064972032353
1101	52-53	0.5084285153844377
1101	54-55	0.4757786890154918
1101	56-57	0.4418646422453847
1101	58-59	0.43378528147033535
1101	60-61	0.4999472985988902
1101	62-63	0.4692609162423125
1101	64-65	0.5178016376628207
1101	66-67	0.49795945140445497
1101	68-69	0.4928123402920832
1101	70-71	0.475769235764929
1101	72-73	0.4607629786402896
1101	74-75	0.4210932814873729
1101	76-77	0.3807265905675479
1101	78-79	0.3943833895061388
1101	80-81	0.3764055294003654
1101	82-83	0.3960431893952645
1101	84-85	0.4133472221085839
1101	86-87	0.4501293719262236
1101	88-89	0.4206461963744843
1101	90-91	0.42477906114825004
1101	92-93	0.4213015310101511
1101	94-95	0.3923433468195938
1101	96-97	0.49230177479695314
1101	98-99	0.5310941483017295
1101	100	0.5719799663301508
1102	1	0.22992020298702087
1102	2	0.3305489605980867
1102	3	0.3714201011707736
1102	4	0.3595017777753071
1102	5	0.26813960866843445
1102	6	0.25092730882574443
1102	7	0.27692572229160106
1102	8	0.28406773278738484
1102	9	0.3196740545141523
1102	10-11	0.31234428239675793
1102	12-13	0.2858485189223998
1102	14-15	0.3846015684493267
1102	16-17	0.3698673150817626
1102	18-19	0.41473225173128725
1102	20-21	0.3869691474912571
1102	22-23	0.40073976271238365
1102	24-25	0.3662423404802624
1102	26-27	0.35820705412506726
1102	28-29	0.36685541197061156
1102	30-31	0.38224427353181056
1102	32-33	0.44043420924731436
1102	34-35	0.4812738316075098
1102	36-37	0.48664332661984844
1102	38-39	0.4738032030271171
1102	40-41	0.4863443551581952
1102	42-43	0.465985586727065
1102	44-45	0.48274653688182667
1102	46-47	0.5542503646834049
1102	48-49	0.5504811153954563
1102	50-51	0.5514239924910882
1102	52-53	0.5416116337922006
1102	54-55	0.5006832275351698
1102	56-57	0.48696056253351117
1102	58-59	0.5125481883409222
1102	60-61	0.503737283163673
1102	62-63	0.48822822288338585
1102	64-65	0.509039591280029
1102	66-67	0.5047774335998625
1102	68-69	0.5155933529326902
1102	70-71	0.5047211687739903
1102	72-73	0.5097259837350236
1102	74-75	0.5267530596100585
1102	76-77	0.4841929951246371
1102	78-79	0.4758142994773351
1102	80-81	0.4312541281799014
1102	82-83	0.4377356751588337
1102	84-85	0.4169681886613148
1102	86-87	0.4329709864726823
1102	88-89	0.4452050000036678
1102	90-91	0.4333458464265476
1102	92-93	0.48393834767662014
1102	94-95	0.46564911865750247
1102	96-97	0.5191094284609754
1102	98-99	0.5190925974599345
1102	100	0.5664116552034706
1103	1	0.2566458838414327
1103	2	0.2834332321697346
1103	3	0.38676995396017944
1103	4	0.3314493981570834
1103	5	0.2097690936649812
1103	6	0.25882956523086875
1103	7	0.236396568402661
1103	8	0.2791035011757472
1103	9	0.3072409724238483
1103	10-11	0.31538471164052595
1103	12-13	0.28672705879036897
1103	14-15	0.37308509805671264
1103	16-17	0.3573368532863128
1103	18-19	0.3535443996706391
1103	20-21	0.37721155448048194
1103	22-23	0.3549143180845249
1103	24-25	0.40402998692486136
1103	26-27	0.43823867868697164
1103	28-29	0.4052140095203498
1103	30-31	0.4485601090276603
1103	32-33	0.41094351799196716
1103	34-35	0.46418051498636714
1103	36-37	0.5189701183125734
1103	38-39	0.5185974594950835
1103	40-41	0.4873937507997539
1103	42-43	0.4985268505694478
1103	44-45	0.48127518176986683
1103	46-47	0.5041397040570601
1103	48-49	0.4575148908081701
1103	50-51	0.4903226404072498
1103	52-53	0.45814359613826383
1103	54-55	0.4388884678418705
1103	56-57	0.4872073562118686
1103	58-59	0.4574669718547426
1103	60-61	0.45205776498936956
1103	62-63	0.4400232257281331
1103	64-65	0.4406032303125045
1103	66-67	0.46168095260214415
1103	68-69	0.4857798145828198
1103	70-71	0.5268014871880453
1103	72-73	0.48664985613167744
1103	74-75	0.46409181654934173
1103	76-77	0.4503065145999372
1103	78-79	0.3822598728819031
1103	80-81	0.3831385837611805
1103	82-83	0.41750629308870657
1103	84-85	0.44186476843281497
1103	86-87	0.47103615262339105
1103	88-89	0.48030320575958996
1103	90-91	0.5071669076573428
1103	92-93	0.4804304691361452
1103	94-95	0.48299286034610134
1103	96-97	0.473098468943153
1103	98-99	0.49308176240562673
1103	100	0.49523141158881145
1104	1	0.2170585248901027
1104	2	0.24609564522453553
1104	3	0.31783997040960443
1104	4	0.2495655548051161
1104	5	0.17031095251557105
1104	6	0.18207333995502495
1104	7	0.15975102711936984
1104	8	0.1824913950505831
1104	9	0.23376218740575183
1104	10-11	0.24170001385572704
1104	12-13	0.2463270798133408
1104	14-15	0.3318785662995296
1104	16-17	0.3286283008146711
1104	18-19	0.2737799344319498
1104	20-21	0.2939384502624307
1104	22-23	0.284777993856963
1104	24-25	0.28217640632953334
1104	26-27	0.33565775199137704
1104	28-29	0.3380248584638039
1104	30-31	0.3536986661330346
1104	32-33	0.37110229635505476
1104	34-35	0.3394024251839767
1104	36-37	0.3178757615545109
1104	38-39	0.34452801532457755
1104	40-41	0.31137858180036204
1104	42-43	0.31828889250736836
1104	44-45	0.3365967756356909
1104	46-47	0.37010925159004415
1104	48-49	0.33215475014714
1104	50-51	0.3869238358677123
1104	52-53	0.4179769501419699
1104	54-55	0.3488831943193418
1104	56-57	0.3816252460990057
1104	58-59	0.4053604328493847
1104	60-61	0.3961167475543519
1104	62-63	0.4358904975576934
1104	64-65	0.4493044906053143
1104	66-67	0.4458243239803892
1104	68-69	0.39318745925825027
1104	70-71	0.37990117202542706
1104	72-73	0.4253187897666493
1104	74-75	0.4482025756179979
1104	76-77	0.4211609820854463
1104	78-79	0.37136204486102997
1104	80-81	0.3906836498186479
1104	82-83	0.344058888943799
1104	84-85	0.40095707446188555
1104	86-87	0.40604272873580527
1104	88-89	0.3632568380219823
1104	90-91	0.35376647630732094
1104	92-93	0.36486220901593214
1104	94-95	0.3614814979967562
1104	96-97	0.37052269283401174
1104	98-99	0.43184269402378206
1104	100	0.44458116025506556
1105	1	0.18502396438426416
1105	2	0.21708418640589144
1105	3	0.31363119040521426
1105	4	0.24644844048815884
1105	5	0.19134161020412677
1105	6	0.22202504314101645
1105	7	0.22445113010464013
1105	8	0.2214605594305752
1105	9	0.2519515509876058
1105	10-11	0.23792565117857833
1105	12-13	0.2618676856725841
1105	14-15	0.3454023290616064
1105	16-17	0.29192378380645323
1105	18-19	0.28218250255828536
1105	20-21	0.2672874902908262
1105	22-23	0.3065197588966271
1105	24-25	0.29540051003538537
1105	26-27	0.28436470791726265
1105	28-29	0.3478840435780981
1105	30-31	0.3245429637755777
1105	32-33	0.3648461967044483
1105	34-35	0.34968404205701376
1105	36-37	0.4094989207287796
1105	38-39	0.4177013761550441
1105	40-41	0.4150948290222942
1105	42-43	0.3476614000407636
1105	44-45	0.3982054816827727
1105	46-47	0.42204955274332434
1105	48-49	0.4684204643800385
1105	50-51	0.4860934879849239
1105	52-53	0.45345835708234716
1105	54-55	0.4748774549385857
1105	56-57	0.46587859032562307
1105	58-59	0.49932688642053336
1105	60-61	0.5254616294106782
1105	62-63	0.488404111711624
1105	64-65	0.4835895929504659
1105	66-67	0.5061379999340687
1105	68-69	0.5139417311923395
1105	70-71	0.5167586836249498
1105	72-73	0.5217150560768502
1105	74-75	0.4724527342763025
1105	76-77	0.42998144769502034
1105	78-79	0.3903682328737901
1105	80-81	0.39822619387396685
1105	82-83	0.40280666227884154
1105	84-85	0.37973538519985084
1105	86-87	0.4472053877500528
1105	88-89	0.40010880662681103
1105	90-91	0.3975162156526224
1105	92-93	0.3542314793875079
1105	94-95	0.4089922253947549
1105	96-97	0.4152319258856103
1105	98-99	0.48636301116641434
1105	100	0.48567018938201656
1106	1	0.14557460458622273
1106	2	0.19015045923698892
1106	3	0.2658653988978621
1106	4	0.23696693724933482
1106	5	0.16041050173269156
1106	6	0.15985968203472112
1106	7	0.17813733773321871
1106	8	0.20831187665449846
1106	9	0.14505411624180908
1106	10-11	0.2499247941830518
1106	12-13	0.1803130836988771
1106	14-15	0.29375381991263794
1106	16-17	0.279312970532942
1106	18-19	0.2766984320623038
1106	20-21	0.22167781606647452
1106	22-23	0.23888236432634358
1106	24-25	0.2617242027370352
1106	26-27	0.28807059950108993
1106	28-29	0.29587731698625674
1106	30-31	0.2884664742242151
1106	32-33	0.29307466034352103
1106	34-35	0.30285065756736884
1106	36-37	0.28866968560725326
1106	38-39	0.31711526207388374
1106	40-41	0.32345882051110664
1106	42-43	0.2857322271073599
1106	44-45	0.3342093760998708
1106	46-47	0.34814899849651937
1106	48-49	0.32292470150210306
1106	50-51	0.4213993567163996
1106	52-53	0.4045229769434613
1106	54-55	0.3870079668439175
1106	56-57	0.4234486247774498
1106	58-59	0.3952863788500167
1106	60-61	0.3734353362117915
1106	62-63	0.4323459207637157
1106	64-65	0.4028920700938414
1106	66-67	0.44296710941264195
1106	68-69	0.4231287065192859
1106	70-71	0.39227810936593244
1106	72-73	0.38351480198102195
1106	74-75	0.36971798104686826
1106	76-77	0.3572366446369628
1106	78-79	0.33391842686322804
1106	80-81	0.3296683971677936
1106	82-83	0.3138506648646171
1106	84-85	0.3539322741993516
1106	86-87	0.39987517347834256
1106	88-89	0.3686555500968538
1106	90-91	0.3454403307820755
1106	92-93	0.33074613683701415
1106	94-95	0.363414598482116
1106	96-97	0.344522204281521
1106	98-99	0.3792500447462963
1106	100	0.42066884052069753
1107	1	0.10701493432697973
1107	2	0.1649818155464473
1107	3	0.19165425054234575
1107	4	0.20422635101417796
1107	5	0.16954666061001689
1107	6	0.1474772015943273
1107	7	0.15183697184804856
1107	8	0.19283552966083306
1107	9	0.18915745143456775
1107	10-11	0.1587212905060582
1107	12-13	0.13241581842390104
1107	14-15	0.22008214045517605
1107	16-17	0.1838431425933038
1107	18-19	0.19923838402550587
1107	20-21	0.24223331780471824
1107	22-23	0.19233500735920472
1107	24-25	0.20213199781679947
1107	26-27	0.18537589610124883
1107	28-29	0.24350455383815728
1107	30-31	0.20939575696535684
1107	32-33	0.2355375460872935
1107	34-35	0.21549267751120738
1107	36-37	0.2839686974722113
1107	38-39	0.2773335450769565
1107	40-41	0.2243986558922444
1107	42-43	0.2326042779559998
1107	44-45	0.28345287796850016
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2216	88-89	-1.0960708552303728
2216	90-91	-0.9521413511867536
2216	92-93	-1.0048794002075638
2216	94-95	-1.1478947767795944
2216	96-97	-1.1992017227118623
2216	98-99	-1.210914601973812
2216	100	-1.187477820538824
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	565.0
3	63.0
4	68.0
5	117.0
6	351.0
7	994.0
8	2410.0
9	4083.0
10	5628.0
11	7783.0
12	11343.0
13	15741.0
14	19448.0
15	25546.0
16	33709.0
17	48147.0
18	48172.0
19	26919.0
20	19763.0
21	19418.0
22	20400.0
23	22667.0
24	26548.0
25	33115.0
26	42482.0
27	55115.0
28	73804.0
29	97075.0
30	126936.0
31	164350.0
32	211824.0
33	277524.0
34	377280.0
35	547772.0
36	903998.0
37	1671039.0
38	2362995.0
39	939856.0
40	2818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.79718726851026	20.895748365301817	15.19953278046335	23.10753158572457
2	15.299280345961591	20.676741047244786	39.63971467965235	24.384263927141273
3	17.9172164048252	25.146892541658655	31.05854290067273	25.877348152843417
4	12.848165416582201	17.514478442264064	37.9368204600327	31.700535681121032
5	12.809791600638334	37.66633986936029	35.44844261239649	14.075425917604885
6	30.400289243888395	38.14900885833657	17.35230538509412	14.098396512680914
7	26.47596360076655	33.105229886130545	22.207150310152954	18.21165620294995
8	24.974229697718172	36.23505765006366	20.120562094486015	18.670150557732146
9	24.55104739845371	17.040678653285262	21.76717511345968	36.64109883480135
10-11	23.72068726625772	27.26236095494277	28.51254494192801	20.5044068368715
12-13	24.49695002833158	24.6421209565362	29.01823605591921	21.842692959213014
14-15	21.666866920558693	26.689179973042716	26.775747851291904	24.868205255106687
16-17	20.65427590603436	30.0314166112786	27.56313572504694	21.751171757640098
18-19	20.549430604231446	28.389476744651283	30.429822695955533	20.631269955161734
20-21	21.530829914065045	27.51357745142804	30.34463702489832	20.610955609608595
22-23	21.228406232593983	27.34380020237962	30.470033097045953	20.957760467980442
24-25	21.119941812827705	27.446481308013492	30.388624160479814	21.04495271867899
26-27	20.901212507574687	27.46611669006262	30.409793272587116	21.22287752977558
28-29	20.902329315136356	27.471887079468615	30.595948388268535	21.02983521712649
30-31	21.221560964958037	27.717024838198554	30.183506822179694	20.877907374663707
32-33	20.936534541759265	27.56782679928847	30.482320605533715	21.013318053418555
34-35	21.108484303697466	27.403833912917598	30.39004270922927	21.097639074155666
36-37	21.32694081111405	27.561923290218342	30.00912599695485	21.10200990171276
38-39	21.20012012804282	27.68665858538439	30.07543405773081	21.03778722884198
40-41	21.162189104430166	27.536844075788817	30.106339021511747	21.194627798269273
42-43	21.146263772956544	27.537977702353555	29.975747666123574	21.340010858566323
44-45	21.324121900137563	27.658505848664372	29.74700364918635	21.27036860201172
46-47	21.394243068449466	27.828070921617787	29.410413554245423	21.367272455687324
48-49	21.442649528981196	27.81794102862486	29.135390899900653	21.604018542493293
50-51	21.495378319676874	27.917694104147667	28.928016046817444	21.65891152935802
52-53	21.49218961607766	27.990209831246045	28.849874622114374	21.667725930561918
54-55	21.56592384017878	28.18061665890304	28.701527158661406	21.551932342256773
56-57	21.441588648506173	27.997466253695197	28.804256761688418	21.756688336110212
58-59	21.360406486489634	27.73017480553419	29.092081076634386	21.817337631341786
60-61	21.500118939856687	27.91004364037922	28.871425651192684	21.71841176857141
62-63	21.315143820231803	27.952624351559543	28.923008690975337	21.809223137233317
64-65	21.325243402354015	27.77234741689547	29.046743727407794	21.855665453342716
66-67	21.295441754267102	27.80671388211205	28.93423583748815	21.9636085261327
68-69	21.54567011636707	27.954685490768156	28.723854146030014	21.775790246834756
70-71	21.484139048815777	27.75438519490981	28.76384630885128	21.99762944742313
72-73	21.368436393122778	27.875022460355197	28.83869597299471	21.917845173527308
74-75	21.400996330444748	27.95064808278893	28.677642192538045	21.970713394228277
76-77	21.432937926004133	27.920967678184876	28.552652286057995	22.093442109752996
78-79	21.468552376696152	27.9277817256046	28.662051245553844	21.9416146521454
80-81	21.435081788348676	27.874158863465343	28.631714459664515	22.059044888521466
82-83	21.326487094344426	27.85028803004424	28.779537666691418	22.043687208919913
84-85	21.357415360530833	27.785205288252747	28.618675424649236	22.238703926567187
86-87	21.44979683229577	27.886492093833727	28.533798924473313	22.129912149397192
88-89	21.33212647453436	27.74365174570173	28.593920991365273	22.330300788398635
90-91	21.222774298154224	27.720781841687643	28.57565217834107	22.480791681817063
92-93	21.26328194691859	27.85438303111647	28.490594919070865	22.391740102894072
94-95	21.100987953105857	27.672071272733533	28.64245001749252	22.58449075666809
96-97	21.101712855179752	27.69450303872541	28.637686402761638	22.566097703333202
98-99	21.112874934998313	27.670185598378634	28.594455315273787	22.622484151349266
100	21.14857573795615	27.584477277521952	28.54863975518261	22.71830722933929
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	655.0
1	514.0
2	626.0
3	956.0
4	745.0
5	527.0
6	1294.0
7	3559.5
8	6034.0
9	7143.0
10	6042.5
11	4468.0
12	4318.0
13	4806.5
14	5544.0
15	6371.5
16	7130.0
17	7981.5
18	9051.0
19	10240.0
20	12058.0
21	14645.5
22	18938.0
23	25730.0
24	34107.0
25	44094.0
26	57740.0
27	75671.0
28	98898.5
29	124053.0
30	146652.5
31	169635.0
32	191131.0
33	210955.5
34	230958.0
35	248732.0
36	262665.0
37	276063.0
38	288770.0
39	296214.0
40	300830.0
41	301923.0
42	297261.0
43	293466.5
44	288999.5
45	281989.5
46	275633.5
47	266201.5
48	257349.5
49	253313.5
50	248138.5
51	238997.0
52	225919.5
53	214696.5
54	207215.0
55	196205.0
56	181252.5
57	162971.0
58	145601.0
59	131522.5
60	114512.5
61	94890.0
62	78628.0
63	64353.0
64	51266.5
65	40782.5
66	32012.5
67	24426.5
68	18232.0
69	13428.0
70	9559.5
71	6830.5
72	4904.5
73	3312.0
74	2219.5
75	1449.5
76	862.5
77	515.5
78	325.5
79	198.5
80	122.0
81	60.0
82	34.5
83	24.5
84	10.5
85	5.0
86	4.0
87	3.5
88	1.5
89	1.0
90	0.5
91	0.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06134920232700192
2	1.6974087600356261E-4
3	0.0
4	0.1182851418779112
5	0.0017459061531795014
6	0.0018792739843251575
7	0.0
8	0.0
9	2.4248696571937516E-5
10-11	0.0
12-13	3.03108707149219E-5
14-15	3.6373044857906275E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.5155435357460948E-4
30-31	3.091708812922033E-4
32-33	1.8186522428953137E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.8186522428953139E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.0911913457371884E-4
80-81	2.7279783643429707E-4
82-83	3.4554392615010964E-4
84-85	0.0
86-87	0.0
88-89	9.699478628775007E-5
90-91	8.487043800178131E-5
92-93	7.698961161590162E-4
94-95	6.729013298712661E-4
96-97	0.0
98-99	3.6373044857906275E-5
100	0.0010426939525933132
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8247866.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.63737691667334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.71295000616635	40.455578062163035
2	15.816643673264647	17.59993131212284
3	5.103337557945474	8.51808945733283
4	2.190796039393339	4.875605799651294
5	1.172969629871124	3.2630476704475284
6	0.7215692502913249	2.408773220996391
7	0.4310911371844684	1.678934605947872
8	0.32646397705959407	1.453087947310467
9	0.24350470086507794	1.2193166540710922
>10	1.1826035431603426	11.649182478258227
>50	0.0672558628565064	2.5317459606599595
>100	0.029027299503903835	2.815925921301701
>500	0.001242572402907767	0.4570856372319903
>1k	4.575900543897372E-4	0.36749616307044475
>5k	2.1789995155535604E-5	0.07578325922429861
>10k+	6.536998546660681E-5	0.6304158502101308
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20470	0.24818540941378048	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20067	0.24329929705453507	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11463	0.1389814044020599	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.2124348285968758E-5	0.0
4	0.0	0.0	0.0	1.2124348285968758E-5	0.0
5	0.0	1.2124348285968758E-5	0.0	1.2124348285968758E-5	0.0
6	0.0	1.2124348285968758E-5	0.0	1.2124348285968758E-5	0.0
7	0.0	1.2124348285968758E-5	0.0	1.2124348285968758E-5	0.0
8	0.0	1.2124348285968758E-5	0.0	1.2124348285968758E-5	0.0
9	3.6373044857906275E-5	1.2124348285968758E-5	0.0	7.274608971581255E-5	0.0
10-11	4.2435219000890654E-5	1.2124348285968758E-5	0.0	9.093261214476568E-5	0.0
12-13	4.849739314387503E-5	1.2124348285968758E-5	0.0	1.0911913457371882E-4	0.0
14-15	4.849739314387503E-5	2.4248696571937516E-5	0.0	1.3943000528864074E-4	0.0
16-17	4.849739314387503E-5	3.6373044857906275E-5	0.0	1.8792739843251577E-4	0.0
18-19	4.849739314387503E-5	5.455956728685941E-5	0.0	2.061139208614689E-4	0.0
20-21	4.849739314387503E-5	6.062174142984379E-5	0.0	2.2430044329042203E-4	0.0
22-23	4.849739314387503E-5	6.668391557282816E-5	0.0	3.3341957786414087E-4	0.0
24-25	4.849739314387503E-5	7.274608971581255E-5	0.0	5.092226280106879E-4	0.0
26-27	4.849739314387503E-5	7.880826385879693E-5	0.0	9.2751264387661E-4	0.0
28-29	4.849739314387503E-5	8.487043800178131E-5	0.0	0.0031220196836369554	0.0
30-31	4.849739314387503E-5	8.487043800178131E-5	0.0	0.009353934702624897	0.0
32-33	4.849739314387503E-5	8.487043800178131E-5	0.0	0.022896831738052	0.0
34-35	7.274608971581255E-5	8.487043800178131E-5	0.0	0.045302627370522264	0.0
36-37	7.274608971581255E-5	8.487043800178131E-5	0.0	0.07962059519395684	0.0
38-39	7.274608971581255E-5	1.0305696043073445E-4	0.0	0.1267479369815174	0.0
40-41	7.274608971581255E-5	1.0911913457371882E-4	0.0	0.1758454853655479	0.0
42-43	7.274608971581255E-5	1.0911913457371882E-4	0.0	0.22617971727474717	0.0
44-45	7.880826385879693E-5	1.0911913457371882E-4	0.0	0.2897113022932235	0.0
46-47	8.487043800178131E-5	1.0911913457371882E-4	0.0	0.3661674425845425	0.0
48-49	8.487043800178131E-5	1.0911913457371882E-4	0.0	0.44736420305567526	0.0
50-51	8.487043800178131E-5	1.0911913457371882E-4	0.0	0.5293793570361109	0.0
52-53	8.487043800178131E-5	1.0911913457371882E-4	0.0	0.6109883453489666	0.0
54-55	8.487043800178131E-5	1.0911913457371882E-4	0.0	0.7038851019160592	0.0
56-57	9.699478628775007E-5	1.0911913457371882E-4	0.0	0.8146288991600978	0.0
58-59	1.0911913457371882E-4	1.0911913457371882E-4	0.0	0.9377213451333957	0.0
60-61	1.0911913457371882E-4	1.0911913457371882E-4	0.0	1.0647663286479192	0.0
62-63	1.0911913457371882E-4	1.0911913457371882E-4	0.0	1.1892470124999606	0.0
64-65	1.151813087167032E-4	1.0911913457371882E-4	0.0	1.3255368115825354	0.0
66-67	1.2124348285968758E-4	1.0911913457371882E-4	0.0	1.4822318888303956	0.0
68-69	1.2124348285968758E-4	1.0911913457371882E-4	0.0	1.657549965045504	0.0
70-71	1.2124348285968758E-4	1.2730565700267197E-4	0.0	1.842215913789094	0.0
72-73	1.2124348285968758E-4	1.3336783114565635E-4	0.0	2.033259269731104	0.0
74-75	1.3336783114565635E-4	1.3336783114565635E-4	0.0	2.228303660607483	0.0
76-77	1.3336783114565635E-4	1.3336783114565635E-4	0.0	2.439964470809783	0.0
78-79	1.3336783114565635E-4	1.3336783114565635E-4	0.0	2.682863664370881	0.0
80-81	1.3336783114565635E-4	1.3336783114565635E-4	0.0	2.9439008829677884	0.0
82-83	1.3336783114565635E-4	1.3336783114565635E-4	0.0	3.2146193936710414	0.0
84-85	1.5761652771759387E-4	1.3336783114565635E-4	0.0	3.4870413753084737	0.0
86-87	1.5761652771759387E-4	1.3336783114565635E-4	0.0	3.776825423691413	0.0
88	1.5761652771759387E-4	1.3336783114565635E-4	0.0	4.0138261218113875	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14900	0.0	56.250774	1
GTATCAA	26385	0.0	41.116882	1
ATCAACG	32480	0.0	32.916637	3
TCAACGC	32800	0.0	32.68158	4
TATCAAC	33795	0.0	31.858603	2
CAACGCA	34080	0.0	31.401411	5
GTGGTAT	6155	0.0	30.370867	1
AACGCAG	36035	0.0	29.801777	6
ACGCAGA	40910	0.0	26.134958	7
CGCAGAG	40925	0.0	25.953125	8
TGGTATC	7020	0.0	25.472387	2
GCAGAGT	44410	0.0	23.239218	9
GTACATG	24070	0.0	21.77278	1
GAGTACT	29500	0.0	21.507092	12-13
CAGAGTA	43035	0.0	20.650932	10-11
TACATGG	24650	0.0	20.579035	2
AGAGTAC	40680	0.0	20.211704	10-11
ACATGGG	25270	0.0	19.44099	3
GTACTTT	32175	0.0	19.397665	14-15
AGTACTT	31105	0.0	19.082844	12-13
>>END_MODULE