FastQCFastQC Report
Wed 25 May 2016
SRR2049525_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049525_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8139030
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85450.042.459081
GTATCAA124500.036.005691
TCAACGC134950.031.885054
ATCAACG135650.031.8336643
CAACGCA147150.029.1717625
TATCAAC150750.028.9165342
AACGCAG159050.026.9887776
GTACATG251550.024.6758941
TACATGG256000.023.623352
ACATGGG262300.022.1976993
ACGCAGA192050.022.0841187
GTGGTAT42750.021.5463031
CGCAGAG200400.021.2679088
GTACCGT5800.018.5244886
CATGGGG189200.018.4698564
GCAGAGT230550.017.3679969
GAGTACA193000.016.8100241
TATACCG6150.016.6385655
CAGAGTA213150.015.9413910-11
AGAGTAC195350.015.56422310-11
Produced by FastQC (version 0.11.5)