##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049525_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8139030 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.84701014740086 31.0 31.0 34.0 28.0 34.0 2 31.019414844275055 31.0 31.0 34.0 28.0 34.0 3 31.10910710490071 31.0 31.0 34.0 28.0 34.0 4 34.73557438662838 37.0 35.0 37.0 32.0 37.0 5 34.768407783237066 37.0 35.0 37.0 32.0 37.0 6 34.83956577135113 37.0 35.0 37.0 32.0 37.0 7 30.901408398789535 37.0 33.0 37.0 2.0 37.0 8 32.66480698559902 37.0 33.0 37.0 17.0 37.0 9 35.50277416350597 39.0 35.0 39.0 30.0 39.0 10-11 36.03857744718965 39.0 36.0 39.0 31.5 39.0 12-13 36.1998642344358 39.0 37.0 39.0 32.0 39.0 14-15 37.40929901966204 40.0 38.0 41.0 32.0 41.0 16-17 37.42395193284703 40.0 38.0 41.0 32.0 41.0 18-19 37.415129628469245 40.0 38.0 41.0 31.5 41.0 20-21 37.47938710141135 40.0 38.0 41.0 32.0 41.0 22-23 37.444601199405824 40.0 38.0 41.0 31.5 41.0 24-25 37.41633032437527 40.0 38.0 41.0 31.0 41.0 26-27 37.440721744483064 40.0 38.0 41.0 32.0 41.0 28-29 37.425236852548764 40.0 38.0 41.0 32.0 41.0 30-31 37.355946408847245 40.0 37.0 41.0 31.0 41.0 32-33 37.31210764673432 40.0 37.0 41.0 31.0 41.0 34-35 37.26071073580021 40.0 37.0 41.0 31.0 41.0 36-37 37.097781123794846 40.0 37.0 41.0 30.5 41.0 38-39 36.998081958169465 40.0 37.0 41.0 30.0 41.0 40-41 36.92937659647403 40.0 37.0 41.0 30.0 41.0 42-43 36.82306110187578 40.0 37.0 41.0 30.0 41.0 44-45 36.71561420709839 40.0 36.0 41.0 30.0 41.0 46-47 36.56124390744351 40.0 36.0 41.0 30.0 41.0 48-49 36.48009308234519 40.0 36.0 41.0 30.0 41.0 50-51 36.33437172488614 40.0 35.5 41.0 30.0 41.0 52-53 36.2170059577124 39.0 35.0 41.0 29.5 41.0 54-55 36.09009789864395 39.0 35.0 41.0 29.0 41.0 56-57 35.881766623295405 39.0 35.0 41.0 29.0 41.0 58-59 35.69234878112011 39.0 35.0 41.0 28.5 41.0 60-61 35.46073967045213 38.5 35.0 40.0 28.0 41.0 62-63 35.215806920480695 38.0 34.5 40.0 28.0 41.0 64-65 34.848134851450354 37.5 34.0 40.0 27.5 41.0 66-67 31.32625767444032 36.0 31.0 40.0 6.5 41.0 68-69 31.688394378691317 36.0 31.0 39.0 11.0 41.0 70-71 31.843281508975885 35.0 30.0 39.0 15.5 41.0 72-73 33.07817043308601 35.0 32.5 39.0 25.5 40.5 74-75 33.156151715867864 35.0 33.0 38.0 26.0 40.0 76-77 32.86234262559543 35.0 33.0 37.0 26.0 39.0 78-79 29.482403861393802 35.0 29.0 36.5 6.5 39.0 80-81 31.304338600545762 35.0 30.5 36.0 21.5 38.5 82-83 31.722333681040617 35.0 32.0 36.0 24.0 37.0 84-85 29.89355869188343 35.0 30.5 35.5 13.0 37.0 86-87 29.547383287689073 35.0 29.0 35.0 13.5 36.5 88-89 30.835079671656203 35.0 30.5 35.0 22.5 36.0 90-91 31.009925691390745 35.0 32.0 35.0 22.5 36.0 92-93 30.883201560873964 35.0 32.0 35.0 20.0 36.0 94-95 30.698346731735846 35.0 32.0 35.0 19.0 35.5 96-97 30.525809525213692 35.0 32.0 35.0 17.5 35.0 98-99 30.3579680871062 35.0 32.0 35.0 6.5 35.0 100 30.15568587411522 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.32520729374195767 1101 2 0.30819803842954485 1101 3 0.299560515583682 1101 4 0.3058703038092929 1101 5 0.2876627056837435 1101 6 0.36727112685475305 1101 7 -12.096401540844095 1101 8 -6.516190856222181 1101 9 -2.2353117869189063 1101 10-11 -0.12968803056428868 1101 12-13 0.35850325463486854 1101 14-15 0.5698815952363461 1101 16-17 0.6296498984790304 1101 18-19 0.6132361488258766 1101 20-21 0.5629156303805303 1101 22-23 0.571780980514454 1101 24-25 0.6142920175436117 1101 26-27 0.5855680212786041 1101 28-29 0.5595481511645062 1101 30-31 0.6199238283618556 1101 32-33 0.6598215910151666 1101 34-35 0.6463668598982935 1101 36-37 0.7101116986383147 1101 38-39 0.7197479147089325 1101 40-41 0.6845275122056833 1101 42-43 0.679466301288457 1101 44-45 0.5744680617953506 1101 46-47 0.6298774797247262 1101 48-49 0.569463486230184 1101 50-51 0.6425394650705982 1101 52-53 0.6470176412270661 1101 54-55 0.5551596135631343 1101 56-57 0.5547216799289814 1101 58-59 0.5344298823100644 1101 60-61 0.5648866254499012 1101 62-63 0.5146825821542507 1101 64-65 0.3608876315486782 1101 66-67 -10.243351315626576 1101 68-69 -8.097474574543782 1101 70-71 -6.5892590966194895 1101 72-73 -1.4685705306589085 1101 74-75 -0.1858579772266964 1101 76-77 0.1254586906350994 1101 78-79 3.281451699549624 1101 80-81 1.159172173521899 1101 82-83 0.47564621378607086 1101 84-85 -5.343717577100314 1101 86-87 -5.8370498614416135 1101 88-89 -1.23425306549548 1101 90-91 0.06997191458933827 1101 92-93 0.4211086328394167 1101 94-95 0.4683430135307134 1101 96-97 0.575130933298361 1101 98-99 0.5356411434303112 1101 100 0.6143772917442725 1102 1 0.1664133695621608 1102 2 0.20364957125419636 1102 3 0.2053976166217275 1102 4 0.267845593916924 1102 5 0.26685957354567336 1102 6 0.294329772860479 1102 7 -12.297592490721748 1102 8 -6.691592759504445 1102 9 -2.310821881242262 1102 10-11 -0.27065733572433004 1102 12-13 0.3082874357730674 1102 14-15 0.518364210013111 1102 16-17 0.5291122794555889 1102 18-19 0.5191989318938539 1102 20-21 0.5081224115923249 1102 22-23 0.4618612962208104 1102 24-25 0.4891674679461602 1102 26-27 0.4551676545094878 1102 28-29 0.46192550920648046 1102 30-31 0.49167517916806247 1102 32-33 0.4991020043527712 1102 34-35 0.4870302549016188 1102 36-37 0.6034391356533746 1102 38-39 0.5688148814210763 1102 40-41 0.4899143173469156 1102 42-43 0.4919066149068243 1102 44-45 0.5020441875015393 1102 46-47 0.5181506488977732 1102 48-49 0.4134153932488758 1102 50-51 0.46838360899982234 1102 52-53 0.4364592338590114 1102 54-55 0.42378563048150397 1102 56-57 0.436866028664312 1102 58-59 0.36084377758491826 1102 60-61 0.3140246585913218 1102 62-63 0.303172607952618 1102 64-65 0.1925381822032648 1102 66-67 -10.455738595454736 1102 68-69 -6.791680021810034 1102 70-71 -6.368705229047414 1102 72-73 -1.1872832188062752 1102 74-75 -0.24050196657948675 1102 76-77 -0.05425709733347617 1102 78-79 3.1356862971856962 1102 80-81 0.9800485578636788 1102 82-83 0.3639483948006941 1102 84-85 -5.6092163715450525 1102 86-87 -6.099857684750127 1102 88-89 -1.4419176465211088 1102 90-91 -0.13966945015645393 1102 92-93 0.17183208343331913 1102 94-95 0.17674067981386443 1102 96-97 0.2768466487912491 1102 98-99 0.30088219863976207 1102 100 0.372785441116644 1103 1 0.13639267882984996 1103 2 0.12280076443713028 1103 3 0.14273155586300135 1103 4 0.15714119439724072 1103 5 0.18155218290860375 1103 6 0.2333466652868168 1103 7 -12.287542445267224 1103 8 -6.710274913390162 1103 9 -2.365814814525514 1103 10-11 -0.3491497132644028 1103 12-13 0.2080367537038299 1103 14-15 0.360713737808517 1103 16-17 0.3575618284093238 1103 18-19 0.4038028367182278 1103 20-21 0.3405604812050953 1103 22-23 0.37075039216786365 1103 24-25 0.4609610386394536 1103 26-27 0.43183001729174464 1103 28-29 0.3980686240587161 1103 30-31 0.39698239748519626 1103 32-33 0.41446782698547935 1103 34-35 0.39900850547590494 1103 36-37 0.4469447267493507 1103 38-39 0.43267035875617665 1103 40-41 0.44537840393177675 1103 42-43 0.403223433500699 1103 44-45 0.39509641949937446 1103 46-47 0.4175261080081114 1103 48-49 0.35842127155233783 1103 50-51 0.34282432913988004 1103 52-53 0.29090011260652915 1103 54-55 0.29531209843358397 1103 56-57 0.2896787926434641 1103 58-59 0.2792508183668261 1103 60-61 0.3006907187101291 1103 62-63 0.16256221100933033 1103 64-65 0.11489907397213273 1103 66-67 -10.473158940111961 1103 68-69 -8.397765550268396 1103 70-71 -6.883666918031338 1103 72-73 -1.6903458283945483 1103 74-75 -0.49786656368384286 1103 76-77 -0.1524478706664496 1103 78-79 3.034767441176097 1103 80-81 0.9099197873425773 1103 82-83 0.21761312540499844 1103 84-85 -5.649419832926718 1103 86-87 -6.131281902994729 1103 88-89 -1.5462130703392205 1103 90-91 -0.22965473257212565 1103 92-93 0.20244190719574107 1103 94-95 0.30425988171572627 1103 96-97 0.31445018187080365 1103 98-99 0.35341512785772977 1103 100 0.4315713782200561 1104 1 0.15727608616037614 1104 2 0.1781739531975397 1104 3 0.19738519125367304 1104 4 0.19925303151723028 1104 5 0.2153658181708451 1104 6 0.1913778273041018 1104 7 -12.306806156753808 1104 8 -6.712953717298809 1104 9 -2.388015210346488 1104 10-11 -0.3265478081046851 1104 12-13 0.2721335560163993 1104 14-15 0.4489493638379045 1104 16-17 0.4477233305035426 1104 18-19 0.45981240476639584 1104 20-21 0.46214632583837556 1104 22-23 0.46389761737702884 1104 24-25 0.5578879412387536 1104 26-27 0.48870185390180154 1104 28-29 0.49949259142616853 1104 30-31 0.43850572919487973 1104 32-33 0.48551158359298086 1104 34-35 0.4744792906924573 1104 36-37 0.5447706615245664 1104 38-39 0.5564687584690731 1104 40-41 0.5740246826244118 1104 42-43 0.5272471509571233 1104 44-45 0.50337523736939 1104 46-47 0.5036732629808967 1104 48-49 0.48653195327774057 1104 50-51 0.5185840256167964 1104 52-53 0.5064863866085361 1104 54-55 0.4814415658087938 1104 56-57 0.5243765689795765 1104 58-59 0.40595785009112006 1104 60-61 0.4136439542063073 1104 62-63 0.33751613308484707 1104 64-65 0.2809771879043055 1104 66-67 -10.407862971977767 1104 68-69 -8.303918083598841 1104 70-71 -6.731536611299752 1104 72-73 -1.5694694643594431 1104 74-75 -0.36844091020650893 1104 76-77 -0.058446054334957864 1104 78-79 3.1041485206552544 1104 80-81 0.9899418417250736 1104 82-83 0.33825456852657254 1104 84-85 -5.5459825271128835 1104 86-87 -6.032706476839863 1104 88-89 -1.461829975468472 1104 90-91 -0.1621858415462718 1104 92-93 0.21855902244803005 1104 94-95 0.22301917850711206 1104 96-97 0.29432626224472 1104 98-99 0.32045827519178616 1104 100 0.3998803784383931 1105 1 0.05800154725343987 1105 2 0.09981354162320599 1105 3 0.04380205578650731 1105 4 0.12823687268701178 1105 5 0.12695324800917263 1105 6 0.1940531036393196 1105 7 -12.341615479844329 1105 8 -6.725421265231709 1105 9 -2.463319140165396 1105 10-11 -0.3243770244610502 1105 12-13 0.19324654608207936 1105 14-15 0.3701609676056137 1105 16-17 0.3947874679248784 1105 18-19 0.373071639712407 1105 20-21 0.3679955550624072 1105 22-23 0.3901952554953141 1105 24-25 0.4181210341964672 1105 26-27 0.32757613125139073 1105 28-29 0.32025229647671694 1105 30-31 0.38889908537583295 1105 32-33 0.38424660966512647 1105 34-35 0.4250643741349478 1105 36-37 0.495537508913074 1105 38-39 0.4896329426843664 1105 40-41 0.4398239583628438 1105 42-43 0.4001063606066708 1105 44-45 0.3916547342099008 1105 46-47 0.3524981564774663 1105 48-49 0.31590544556430444 1105 50-51 0.42262603412199695 1105 52-53 0.4249964534878856 1105 54-55 0.40735314997358074 1105 56-57 0.3729418347288771 1105 58-59 0.33343665257682176 1105 60-61 0.27360787649429597 1105 62-63 0.24968597196513542 1105 64-65 0.13049097600492843 1105 66-67 -10.467445234821046 1105 68-69 -8.439638283542827 1105 70-71 -6.7881552151752445 1105 72-73 -1.6471833954861985 1105 74-75 -0.3729863365983661 1105 76-77 -0.1309785183832588 1105 78-79 3.058650879179435 1105 80-81 0.9463560392843426 1105 82-83 0.25976559024468315 1105 84-85 -5.5468259827286985 1105 86-87 -6.045486131587019 1105 88-89 -1.4104524609171598 1105 90-91 -0.1182475044920146 1105 92-93 0.2294465440847837 1105 94-95 0.30843291681498286 1105 96-97 0.36526032531687846 1105 98-99 0.3715990418881141 1105 100 0.40700550830656823 1106 1 0.024762381577616566 1106 2 0.11190772090093404 1106 3 0.0870606071333846 1106 4 0.06165822964262446 1106 5 0.03974518978154862 1106 6 0.06490666711282955 1106 7 -12.33349822946813 1106 8 -6.791552876670298 1106 9 -2.6351165842959787 1106 10-11 -0.5328024237630231 1106 12-13 0.08203506135743766 1106 14-15 0.18615495596749554 1106 16-17 0.19336775600045542 1106 18-19 0.1836119250694992 1106 20-21 0.17559290416949125 1106 22-23 0.23269626623297768 1106 24-25 0.2819475453790261 1106 26-27 0.278695277384287 1106 28-29 0.2760948308340474 1106 30-31 0.25980241190895725 1106 32-33 0.2841106705357177 1106 34-35 0.3231980750916108 1106 36-37 0.369078415249227 1106 38-39 0.32125415662800094 1106 40-41 0.32502761305486416 1106 42-43 0.3013578651309956 1106 44-45 0.20903567608137053 1106 46-47 0.27739476191233337 1106 48-49 0.23989687897260836 1106 50-51 0.28479864452054926 1106 52-53 0.25136818332166655 1106 54-55 0.23280861910873796 1106 56-57 0.22981350931065947 1106 58-59 0.20696891089020397 1106 60-61 0.14305291004426124 1106 62-63 0.06305902528211504 1106 64-65 -0.025281030638097945 1106 66-67 -10.54512931218223 1106 68-69 -8.449063909935965 1106 70-71 -6.94178164266804 1106 72-73 -1.8322318530838082 1106 74-75 -0.6202082801396998 1106 76-77 -0.3434606225825618 1106 78-79 2.832144706834466 1106 80-81 0.6437984597348496 1106 82-83 -0.028026423706457138 1106 84-85 -5.781812776835352 1106 86-87 -6.2352063142015695 1106 88-89 -1.7007035146297227 1106 90-91 -0.43534931262840715 1106 92-93 -0.1009998648365169 1106 94-95 -0.042304573541429136 1106 96-97 0.056560618521107386 1106 98-99 0.10378201675396426 1106 100 0.15230943386836415 1107 1 0.01718267224729786 1107 2 0.03551418649261251 1107 3 0.006511303711608463 1107 4 0.00501611297222837 1107 5 0.06764185582525784 1107 6 0.001782447191047254 1107 7 -12.361686235155425 1107 8 -6.847417398446538 1107 9 -2.703844570849526 1107 10-11 -0.6246106911667013 1107 12-13 -0.024716515247959592 1107 14-15 0.08102624664066838 1107 16-17 0.12663781584270595 1107 18-19 0.16468667622550015 1107 20-21 0.12634209036666277 1107 22-23 0.15466469719645204 1107 24-25 0.2765015700566167 1107 26-27 0.18510011618037936 1107 28-29 0.19098319777101835 1107 30-31 0.19986803790998664 1107 32-33 0.18204199601154158 1107 34-35 0.153419390691937 1107 36-37 0.23558363470241517 1107 38-39 0.22300313433689922 1107 40-41 0.21878595746145635 1107 42-43 0.25983967010964903 1107 44-45 0.17087466351523517 1107 46-47 0.20081622888482542 1107 48-49 0.18098772454214185 1107 50-51 0.23147373581129926 1107 52-53 0.17330687962721214 1107 54-55 0.12846865761861181 1107 56-57 0.09987082030386318 1107 58-59 0.08763826954239562 1107 60-61 0.09014629538013708 1107 62-63 0.07700840185415814 1107 64-65 -0.08391328555057953 1107 66-67 -10.542222111825563 1107 68-69 -8.49647029119053 1107 70-71 -6.958786028696402 1107 72-73 -1.8838019343331887 1107 74-75 -0.6152851854180668 1107 76-77 -0.3368544379537255 1107 78-79 2.8418159516123005 1107 80-81 0.7407210589527615 1107 82-83 0.07296309274930479 1107 84-85 -5.729798263869938 1107 86-87 -6.272892407080953 1107 88-89 -1.7527905771602796 1107 90-91 -0.45655169231936554 1107 92-93 -0.08585321662120649 1107 94-95 -0.005883795038126749 1107 96-97 0.07251196132430238 1107 98-99 0.14126240632637632 1107 100 0.21999919234819743 1108 1 0.01706779379414769 1108 2 0.022014982193532262 1108 3 0.04211488329825386 1108 4 -0.03267673021337458 1108 5 -0.03346057272483449 1108 6 0.03737074612399738 1108 7 -12.432320147157743 1108 8 -6.899623032938848 1108 9 -2.7023722970421957 1108 10-11 -0.5810506495086614 1108 12-13 -0.024511944065217506 1108 14-15 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-0.26178623293400705 2215 92-93 -0.5452526729906637 2215 94-95 -0.47963408257319173 2215 96-97 -0.5405079058472069 2215 98-99 -0.6925981197925353 2215 100 -0.5912685225915624 2216 1 -0.6849789772894823 2216 2 -0.5006295926322402 2216 3 -0.6406550333085619 2216 4 -0.6388806495657207 2216 5 -0.44507351831434505 2216 6 -0.7189407801449974 2216 7 3.3237669601432636 2216 8 1.4359011533547203 2216 9 0.18147579386012325 2216 10-11 -0.49729011365993614 2216 12-13 -0.8548167161033646 2216 14-15 -1.0457618788575616 2216 16-17 -1.2434980002890086 2216 18-19 -1.6266784905436822 2216 20-21 -1.2309365423663792 2216 22-23 -1.0768184518914268 2216 24-25 -1.0475801494102441 2216 26-27 -1.4883649875831324 2216 28-29 -1.710723113456801 2216 30-31 -1.6184812697106779 2216 32-33 -2.5965406259258828 2216 34-35 -1.6102704636129914 2216 36-37 -1.2503461315483975 2216 38-39 -1.504822879954908 2216 40-41 -1.5319410323976825 2216 42-43 -1.1303739721833068 2216 44-45 -1.2904809328940345 2216 46-47 -1.5234557715718182 2216 48-49 -1.5301426876034938 2216 50-51 -2.052837176577931 2216 52-53 -1.7534827715905479 2216 54-55 -1.3914825131570296 2216 56-57 -1.410797919902521 2216 58-59 -1.738047154504656 2216 60-61 -1.8260606999647138 2216 62-63 -1.7566254239884884 2216 64-65 -1.4526138748824309 2216 66-67 1.9780046929841184 2216 68-69 1.1579013625156378 2216 70-71 0.7763824907570793 2216 72-73 -0.9910630540101906 2216 74-75 -1.7720692209776914 2216 76-77 -1.4315530941105195 2216 78-79 1.182657625526609 2216 80-81 -0.3689010858709203 2216 82-83 -1.2335850440693221 2216 84-85 0.6005781622501871 2216 86-87 0.6099473029832012 2216 88-89 -0.6645675499816441 2216 90-91 -0.9632809248140148 2216 92-93 -1.0385257353770534 2216 94-95 -1.0315513914714636 2216 96-97 -1.0572127839476941 2216 98-99 -1.0757555037841016 2216 100 -2.335322559859293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 103417.0 3 20382.0 4 12233.0 5 10973.0 6 10542.0 7 11506.0 8 12767.0 9 14225.0 10 15899.0 11 18044.0 12 19742.0 13 22134.0 14 25399.0 15 27648.0 16 30048.0 17 31267.0 18 30036.0 19 28387.0 20 27234.0 21 28186.0 22 29781.0 23 34373.0 24 41211.0 25 51947.0 26 65648.0 27 80221.0 28 99645.0 29 127613.0 30 158422.0 31 197729.0 32 266008.0 33 379426.0 34 583753.0 35 807940.0 36 918234.0 37 1549093.0 38 1724613.0 39 522401.0 40 903.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.462504288805455 19.943707277844794 14.40971044541641 23.184077987933343 2 15.590719500311408 20.072457598015635 40.24599853369569 24.09082436797726 3 18.74226172565499 24.73039750817276 30.161125661896214 26.36621510427603 4 13.108925956328768 16.748674476360875 37.84980179654175 32.29259777076861 5 13.655206952104512 37.567355638755565 34.98221132911523 13.795226080024692 6 31.45386319487193 36.977292525983785 17.668506019981002 13.900338259163284 7 27.908187394168287 32.34840953031421 21.84454082026767 17.89886225524984 8 25.31977708888861 36.237615076687895 20.14301706014317 18.299590774280322 9 25.1439790736734 17.390831585582063 21.23357451686503 36.231614823879504 10-11 24.303805244605314 26.93831451659473 28.436392297362218 20.321487941437738 12-13 24.516736023825935 24.43234636068426 28.55557726166386 22.495340353825945 14-15 22.03484936165612 26.630476850435493 25.64456083833086 25.690112949577532 16-17 21.76449596347757 29.98164946215434 26.383356625511112 21.870497948856976 18-19 21.833240570436526 29.037968898013645 28.00258753192948 21.126202999620347 20-21 22.7310932629564 28.164566293526377 27.695155319491388 21.409185124025836 22-23 22.599684642990912 27.884319623119687 27.713298004387376 21.802697729502025 24-25 22.330738429518014 28.088420856047957 27.58253747682463 21.998303237609395 26-27 22.093009826378168 28.17155548751562 27.60155994383101 22.133874742275196 28-29 22.095427833537904 28.137425467162547 27.845185482790946 21.9219612165086 30-31 22.323243678915055 28.32213421009629 27.500285660576264 21.854336450412397 32-33 22.1363090201736 28.213115361372676 27.738285963736338 21.91228965471739 34-35 22.309138852065274 27.995286413588456 27.692504465061575 22.003070269284695 36-37 22.451668826527495 28.051416462224754 27.499636780929908 21.997277930317843 38-39 22.26581397664696 28.180372677248318 27.611164553893513 21.942648792211205 40-41 22.21178076502974 28.051186689322925 27.66204326559799 22.074989280049344 42-43 22.10791289917087 28.112623290228672 27.69112941878679 22.088334391813667 44-45 22.191578313384756 28.156197356165265 27.607580331320353 22.044643999129622 46-47 22.24076135722359 28.12197634114225 27.52806600467211 22.10919629696205 48-49 22.19598356941568 28.040987506032707 27.571081493378525 22.19194743117309 50-51 22.10035074363622 28.09292869523294 27.562334799894778 22.244385761236067 52-53 22.136488953253856 28.10462165943421 27.5939080007711 22.164981386540834 54-55 22.15590227590725 28.24420849942671 27.522596153137314 22.077293071528725 56-57 22.153427026676358 28.106319631714 27.483267992743976 22.25698534886567 58-59 22.09299189660842 27.862804267281255 27.690326065261463 22.35387777084886 60-61 22.243248111417405 28.079350448487684 27.466854275782453 22.210547164312462 62-63 22.049714854066593 28.160979208889746 27.504175976478145 22.285129960565513 64-65 22.09194646177164 28.070046536818342 27.52649856271068 22.311508438699327 66-67 22.060193945343624 27.991453437286218 27.52416883640879 22.42418378096137 68-69 22.331761404560986 28.079922212815926 27.39432098205605 22.193995400567033 70-71 22.1165190236269 27.934385449208936 27.494331455818777 22.45476407134539 72-73 22.021668611745632 28.032377286629895 27.567997235012452 22.37795686661202 74-75 22.04074741958177 28.088593719292348 27.49910505282231 22.371553808303574 76-77 22.13913379935181 27.964788677447867 27.406069052985504 22.490008470214818 78-79 22.15573313582891 27.993797516331703 27.530753700188964 22.31971564765042 80-81 22.052765612152267 27.972042106525496 27.547632294317104 22.42755998700513 82-83 21.98650246224917 27.93397214543959 27.652246631498027 22.427278760813213 84-85 22.081563718647697 27.854126371092764 27.52328536464756 22.541024545611982 86-87 22.121265532360937 27.834934440030207 27.568116430481133 22.47568359712772 88-89 21.99245557870717 27.784137867440055 27.63390186698717 22.589504686865602 90-91 21.9926788219397 27.767581317326716 27.575984791215568 22.663755069518015 92-93 22.04809228418445 27.79122452936506 27.55324385287748 22.607439333573012 94-95 22.074822547198593 27.598353927061304 27.63834216588687 22.68848135985323 96-97 22.055991938804308 27.618509393895348 27.65581245162797 22.66968621567237 98-99 22.01288351710814 27.680750051130186 27.584221663385875 22.722144768375795 100 22.084351543485354 27.595735509796445 27.516187329697594 22.803725617020607 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1389.0 1 1263.0 2 1412.5 3 1723.5 4 1732.5 5 1655.0 6 1586.5 7 1566.0 8 1627.5 9 1725.5 10 1665.5 11 1534.0 12 1550.5 13 1704.0 14 1789.0 15 1887.0 16 2204.0 17 2700.0 18 3383.0 19 4409.5 20 5928.0 21 8197.5 22 11956.0 23 17818.5 24 25088.5 25 34198.0 26 46562.5 27 62120.0 28 81857.0 29 103513.0 30 122986.0 31 141327.0 32 160286.0 33 179073.0 34 197906.0 35 217774.5 36 236285.0 37 255077.0 38 273383.5 39 285350.0 40 295705.5 41 302153.5 42 303404.0 43 306869.0 44 306766.5 45 303227.5 46 299254.0 47 290704.0 48 280254.5 49 273341.5 50 264652.5 51 251708.0 52 238143.0 53 226536.5 54 216498.0 55 203389.0 56 185658.5 57 165679.0 58 149262.5 59 135955.0 60 120166.0 61 102236.5 62 86498.5 63 72052.5 64 58491.0 65 47395.5 66 37857.5 67 29308.0 68 22201.0 69 16825.5 70 12431.0 71 8872.5 72 6515.5 73 4674.0 74 3149.5 75 2093.0 76 1391.0 77 903.5 78 586.0 79 386.5 80 272.5 81 192.0 82 133.0 83 108.5 84 88.5 85 70.0 86 56.5 87 43.5 88 34.0 89 33.5 90 30.0 91 27.5 92 28.5 93 21.5 94 19.5 95 19.5 96 20.5 97 17.0 98 13.5 99 28.0 100 48.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.019240622039727092 2 0.0024695817560569256 3 0.0012655070690241958 4 0.0021009874641081307 5 0.001744679648557629 6 0.0036859429194879487 7 11.565039077138186 8 1.2286476398293162E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.870240065462346E-4 18-19 0.0 20-21 0.0 22-23 6.143238199146581E-6 24-25 0.0 26-27 2.334430515675701E-4 28-29 0.0 30-31 0.0 32-33 1.8429714597439743E-5 34-35 5.528914379231923E-5 36-37 3.071619099573291E-5 38-39 4.300266739402607E-5 40-41 0.0 42-43 3.685942919487949E-5 44-45 2.334430515675701E-4 46-47 0.0011180693522446778 48-49 7.617615366941761E-4 50-51 4.5459962673684697E-4 52-53 3.3173486275391543E-4 54-55 4.1774019754196753E-4 56-57 0.0016955337429644566 58-59 3.747375301479415E-4 60-61 0.001498950120591766 62-63 0.00264159242563303 64-65 0.0012040746870327298 66-67 5.7830478570542185 68-69 5.438565775037074 70-71 0.0031453379579630494 72-73 0.0014068015476045672 74-75 0.0034586431061195255 76-77 0.0062845326777269525 78-79 5.37654978541669 80-81 0.005332330756859232 82-83 0.005338473995058379 84-85 5.783170721818202 86-87 0.004134399308025649 88-89 0.002070271273112398 90-91 0.0038395238744666133 92-93 0.0042265478810128475 94-95 0.004754866366139454 96-97 0.0035262187263101377 98-99 0.0060388031497610895 100 0.0069541456414339295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8139030.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.61696140561781 #Duplication Level Percentage of deduplicated Percentage of total 1 88.69646744444896 73.27832627672677 2 7.581610821559918 12.527392972744602 3 1.8930574464903271 4.691959619859263 4 0.7348020425529391 2.4282844796146126 5 0.37462538712529564 1.547520557484759 6 0.22557452345899018 1.1181769019221215 7 0.13563839484556514 0.7844222422453204 8 0.09569112954430999 0.6324568285137787 9 0.06097002258454895 0.4533442202490595 >10 0.19953474613194885 2.4214180387969226 >50 0.001811332023926074 0.09354218308265418 >100 2.167092332860408E-4 0.023155678760131145 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.143238199146582E-6 0.0 14-15 0.0 1.8429714597439743E-5 0.0 2.4572952796586327E-5 0.0 16-17 0.0 4.300266739402607E-5 0.0 3.685942919487949E-5 0.0 18-19 0.0 5.528914379231924E-5 0.0 3.685942919487949E-5 0.0 20-21 0.0 6.143238199146582E-5 0.0 6.75756201906124E-5 0.0 22-23 0.0 6.143238199146582E-5 0.0 1.4743771677951795E-4 0.0 24-25 0.0 7.371885838975897E-5 0.0 2.8258895716074277E-4 0.0 26-27 0.0 8.600533478805214E-5 0.0 6.941859165035637E-4 0.0 28-29 0.0 9.829181118634531E-5 0.0 0.0021747063224978897 0.0 30-31 0.0 9.829181118634531E-5 0.0 0.007249021074992966 0.0 32-33 0.0 9.829181118634531E-5 0.0 0.018128695925681563 0.0 34-35 0.0 9.829181118634531E-5 0.0 0.035950229941405794 0.0 36-37 0.0 9.829181118634531E-5 0.0 0.0645162875674374 0.0 38-39 0.0 9.829181118634531E-5 0.0 0.10525210006597838 0.0 40-41 0.0 9.829181118634531E-5 0.0 0.1479046028826531 0.0 42-43 0.0 9.829181118634531E-5 0.0 0.19128815104502625 0.0 44-45 0.0 9.829181118634531E-5 0.0 0.2484632689644835 0.0 46-47 0.0 9.829181118634531E-5 0.0 0.31965725645439325 6.143238199146582E-6 48-49 0.0 9.829181118634531E-5 0.0 0.3973999358645932 1.2286476398293163E-5 50-51 0.0 1.1057828758463846E-4 0.0 0.47766748617464244 1.2286476398293163E-5 52-53 0.0 1.1057828758463846E-4 0.0 0.558537073828208 1.2286476398293163E-5 54-55 0.0 1.1057828758463846E-4 0.0 0.6501757580448776 1.2286476398293163E-5 56-57 0.0 1.1057828758463846E-4 0.0 0.752245660723698 1.2286476398293163E-5 58-59 0.0 1.1057828758463846E-4 0.0 0.8692252025118472 1.2286476398293163E-5 60-61 0.0 1.2286476398293164E-4 0.0 0.9913097752434872 1.2286476398293163E-5 62-63 0.0 1.2286476398293164E-4 0.0 1.1154523327718415 1.2286476398293163E-5 64-65 0.0 1.2286476398293164E-4 0.0 1.2539454947334019 1.2286476398293163E-5 66-67 0.0 1.2286476398293164E-4 0.0 1.4136205420056198 1.2286476398293163E-5 68-69 0.0 1.2286476398293164E-4 0.0 1.5763487786628136 1.2286476398293163E-5 70-71 0.0 1.2286476398293164E-4 0.0 1.773509865426224 1.2286476398293163E-5 72-73 1.2286476398293163E-5 1.5358095497866454E-4 0.0 1.9851812808160187 1.2286476398293163E-5 74-75 1.2286476398293163E-5 1.5972419317781113E-4 0.0 2.1823300811030304 1.2286476398293163E-5 76-77 1.2286476398293163E-5 1.5972419317781113E-4 0.0 2.3953775818494343 1.2286476398293163E-5 78-79 1.2286476398293163E-5 1.5972419317781113E-4 0.0 2.6465991647653344 1.2286476398293163E-5 80-81 1.2286476398293163E-5 1.5972419317781113E-4 0.0 2.9515740819237672 1.2286476398293163E-5 82-83 1.2286476398293163E-5 1.658674313769577E-4 0.0 3.2705371524616567 1.2286476398293163E-5 84-85 1.2286476398293163E-5 1.7201066957610428E-4 0.0 3.594746548421618 1.2286476398293163E-5 86-87 1.2286476398293163E-5 1.7201066957610428E-4 0.0 3.979276400258999 1.2286476398293163E-5 88 1.2286476398293163E-5 1.7201066957610428E-4 0.0 4.302453732201503 1.2286476398293163E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8545 0.0 42.45908 1 GTATCAA 12450 0.0 36.00569 1 TCAACGC 13495 0.0 31.88505 4 ATCAACG 13565 0.0 31.833664 3 CAACGCA 14715 0.0 29.171762 5 TATCAAC 15075 0.0 28.916534 2 AACGCAG 15905 0.0 26.988777 6 GTACATG 25155 0.0 24.675894 1 TACATGG 25600 0.0 23.62335 2 ACATGGG 26230 0.0 22.197699 3 ACGCAGA 19205 0.0 22.084118 7 GTGGTAT 4275 0.0 21.546303 1 CGCAGAG 20040 0.0 21.267908 8 GTACCGT 580 0.0 18.524488 6 CATGGGG 18920 0.0 18.469856 4 GCAGAGT 23055 0.0 17.367996 9 GAGTACA 19300 0.0 16.810024 1 TATACCG 615 0.0 16.638565 5 CAGAGTA 21315 0.0 15.94139 10-11 AGAGTAC 19535 0.0 15.564223 10-11 >>END_MODULE