FastQCFastQC Report
Wed 25 May 2016
SRR2049525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049525_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8139030
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255780.3142634933155425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241320.2964972484436106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147930.18175384535995076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151050.055.485161
GTATCAA265850.041.4789541
ATCAACG337900.032.1946223
TCAACGC340700.031.8885974
GTGGTAT60100.031.3374081
TATCAAC352450.031.0261292
CAACGCA352650.030.9454945
AACGCAG370450.029.5981276
ACGCAGA423350.025.7326777
CGCAGAG428300.025.1719258
TGGTATC73250.024.602552
GTACATG256850.023.9042761
GCAGAGT461850.022.9363379
TACATGG261250.022.5855272
ACATGGG268100.021.2535253
GAGTACT305200.021.18873412-13
AGAGTAC425300.020.52600910-11
CAGAGTA443800.020.4857810-11
AGTACTT315350.020.03729212-13
GTACTTT327950.019.53973214-15
Produced by FastQC (version 0.11.5)