##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049525_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8139030
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.814092957023135	31.0	31.0	34.0	30.0	34.0
2	31.999035511602735	33.0	31.0	34.0	30.0	34.0
3	32.02323274395106	34.0	31.0	34.0	30.0	34.0
4	35.62900738785826	37.0	35.0	37.0	33.0	37.0
5	35.749107326057285	37.0	35.0	37.0	35.0	37.0
6	35.8156465819637	37.0	35.0	37.0	35.0	37.0
7	35.79488538560492	37.0	35.0	37.0	35.0	37.0
8	35.79263290588682	37.0	35.0	37.0	35.0	37.0
9	37.542574483691546	39.0	37.0	39.0	35.0	39.0
10-11	37.497103217459575	39.0	37.0	39.0	34.5	39.0
12-13	37.47687427125837	39.0	37.0	39.0	34.5	39.0
14-15	38.75426273155401	40.0	38.0	41.0	35.0	41.0
16-17	38.760632839539845	40.0	38.0	41.0	35.0	41.0
18-19	38.82664912157837	40.0	38.0	41.0	35.0	41.0
20-21	38.85285961594933	40.0	38.0	41.0	35.0	41.0
22-23	38.83195448351954	40.0	38.0	41.0	35.0	41.0
24-25	38.79180141859656	40.0	38.0	41.0	35.0	41.0
26-27	38.70311634187367	40.0	38.0	41.0	34.5	41.0
28-29	38.619536480391396	40.0	38.0	41.0	34.0	41.0
30-31	38.51324187280302	40.0	38.0	41.0	34.0	41.0
32-33	38.384944889010114	40.0	38.0	41.0	34.0	41.0
34-35	38.2927643097519	40.0	38.0	41.0	34.0	41.0
36-37	38.16991674683592	40.0	38.0	41.0	34.0	41.0
38-39	38.09392525890677	40.0	38.0	41.0	33.5	41.0
40-41	37.98290957276236	40.0	37.5	41.0	33.0	41.0
42-43	37.848673367710894	40.0	37.0	41.0	33.0	41.0
44-45	37.66814657274884	40.0	37.0	41.0	33.0	41.0
46-47	37.431333217840454	40.0	37.0	41.0	32.5	41.0
48-49	37.205116518798924	40.0	37.0	41.0	32.0	41.0
50-51	36.998601798985874	40.0	36.5	41.0	31.0	41.0
52-53	36.898555110375554	40.0	36.0	41.0	31.0	41.0
54-55	36.76201156648888	39.5	36.0	41.0	31.0	41.0
56-57	36.58460221918337	39.0	35.0	41.0	31.0	41.0
58-59	36.40510847115688	39.0	35.0	41.0	31.0	41.0
60-61	36.20997391581061	39.0	35.0	41.0	30.5	41.0
62-63	36.003565719747925	38.5	35.0	41.0	30.0	41.0
64-65	35.719062713861476	38.0	35.0	40.0	30.0	41.0
66-67	35.45340599555475	37.5	35.0	40.0	29.5	41.0
68-69	35.14081278727318	37.0	35.0	40.0	29.0	41.0
70-71	34.84261767065608	36.5	34.0	39.0	29.0	41.0
72-73	34.51516974381468	36.0	34.0	39.0	29.0	41.0
74-75	34.17696783277614	35.5	34.0	39.0	29.0	40.5
76-77	33.87977793422558	35.0	34.0	37.5	29.0	39.5
78-79	33.55883280931511	35.0	34.0	37.0	29.0	39.0
80-81	33.25828096222769	35.0	34.0	36.5	28.0	39.0
82-83	32.97125701711383	35.0	34.0	36.0	28.0	38.0
84-85	32.710904370668246	35.0	33.0	36.0	27.0	37.0
86-87	32.44691910461074	35.0	33.0	35.5	27.0	37.0
88-89	32.25534148418178	35.0	33.0	35.0	26.5	36.5
90-91	32.122270037092875	35.0	33.0	35.0	27.0	36.0
92-93	31.980432557688076	35.0	33.0	35.0	26.5	36.0
94-95	31.810636464050383	35.0	33.0	35.0	26.0	36.0
96-97	31.657183042696737	35.0	33.0	35.0	26.0	35.5
98-99	31.488214381812085	35.0	33.0	35.0	25.0	35.0
100	31.382580970951082	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.39855455379274574
1101	2	0.38250109843660596
1101	3	0.4833094119036261
1101	4	0.3543609865863715
1101	5	0.28749220528613506
1101	6	0.3035174630255497
1101	7	0.2890496649525218
1101	8	0.2961427065919793
1101	9	0.3299345616969305
1101	10-11	0.34463726350406176
1101	12-13	0.29899660856768406
1101	14-15	0.44031075156342325
1101	16-17	0.457855239232444
1101	18-19	0.41665472417216876
1101	20-21	0.39441291819258595
1101	22-23	0.43225046506064047
1101	24-25	0.49813998907848145
1101	26-27	0.504147558302499
1101	28-29	0.47080852927931716
1101	30-31	0.4844655288208486
1101	32-33	0.47963258259586183
1101	34-35	0.49753984291518094
1101	36-37	0.5301167709747219
1101	38-39	0.5125928885112145
1101	40-41	0.4919438146841699
1101	42-43	0.4422706818193376
1101	44-45	0.5055039322107646
1101	46-47	0.5466182637645218
1101	48-49	0.5360213183066733
1101	50-51	0.5636689543956948
1101	52-53	0.5480136375419278
1101	54-55	0.4434608127128641
1101	56-57	0.46277609530082486
1101	58-59	0.5014975365798335
1101	60-61	0.5092547598893375
1101	62-63	0.49304913311038234
1101	64-65	0.48493550436993615
1101	66-67	0.4255342673350384
1101	68-69	0.4260727688665469
1101	70-71	0.46979108236516964
1101	72-73	0.45126230882025453
1101	74-75	0.47803103598921837
1101	76-77	0.44339540993036053
1101	78-79	0.42117963623365995
1101	80-81	0.42130014160341034
1101	82-83	0.4252733218090583
1101	84-85	0.47884938205290695
1101	86-87	0.5340969521021748
1101	88-89	0.50473845696947
1101	90-91	0.4937297068542037
1101	92-93	0.5171592071882856
1101	94-95	0.46888339110141075
1101	96-97	0.49737406687046715
1101	98-99	0.5302530471522502
1101	100	0.5607390619143402
1102	1	0.2892162687216491
1102	2	0.3126448739813483
1102	3	0.39322475580527083
1102	4	0.3554879306613685
1102	5	0.2890121573563249
1102	6	0.24390288599998655
1102	7	0.2734218810560165
1102	8	0.2923535755375042
1102	9	0.3596681162728146
1102	10-11	0.29782940731305985
1102	12-13	0.2623933231282152
1102	14-15	0.40092005248421003
1102	16-17	0.4050220513537752
1102	18-19	0.43627336788858884
1102	20-21	0.38571261322432804
1102	22-23	0.4033808665897993
1102	24-25	0.40975555152401455
1102	26-27	0.36577690406905816
1102	28-29	0.34703500158654066
1102	30-31	0.43196696575184035
1102	32-33	0.4448771153916127
1102	34-35	0.465714056423451
1102	36-37	0.4689092029800008
1102	38-39	0.519665426647343
1102	40-41	0.5209962440789866
1102	42-43	0.558380194103556
1102	44-45	0.5477619862742671
1102	46-47	0.5358080273802059
1102	48-49	0.558569786536566
1102	50-51	0.5798951779272699
1102	52-53	0.5234948686180232
1102	54-55	0.5204640641961333
1102	56-57	0.5454542834913099
1102	58-59	0.5404410147058627
1102	60-61	0.5578292347126919
1102	62-63	0.5108283469803112
1102	64-65	0.4914463799638469
1102	66-67	0.5318959806006447
1102	68-69	0.47690065250339586
1102	70-71	0.48827105024579254
1102	72-73	0.5472990180390553
1102	74-75	0.5181023809130068
1102	76-77	0.5092587014353569
1102	78-79	0.46502566021649017
1102	80-81	0.46828972624443566
1102	82-83	0.45545800393058755
1102	84-85	0.47640546094805103
1102	86-87	0.5090398523401234
1102	88-89	0.46102668315647577
1102	90-91	0.47399192405107016
1102	92-93	0.5247340060910446
1102	94-95	0.5420266056786538
1102	96-97	0.5992531929690905
1102	98-99	0.6124877052805928
1102	100	0.6170601178581343
1103	1	0.336256134639374
1103	2	0.32033100544060744
1103	3	0.4278208605247755
1103	4	0.33933398890705746
1103	5	0.23829047282254834
1103	6	0.2602301989687561
1103	7	0.280481673007813
1103	8	0.2942941401553938
1103	9	0.3330074126983291
1103	10-11	0.307519317835137
1103	12-13	0.2994478664070499
1103	14-15	0.3857927626803601
1103	16-17	0.3523916379655887
1103	18-19	0.3572476784614764
1103	20-21	0.3285088084369363
1103	22-23	0.37033964067019554
1103	24-25	0.38674177977954116
1103	26-27	0.36323803049927506
1103	28-29	0.3669006094135412
1103	30-31	0.3986870973037071
1103	32-33	0.35209538225447545
1103	34-35	0.353156532078458
1103	36-37	0.4286512574202135
1103	38-39	0.4294962829845872
1103	40-41	0.44471516084393414
1103	42-43	0.37523716001771845
1103	44-45	0.45256700856364773
1103	46-47	0.4737912973551133
1103	48-49	0.4648048242419449
1103	50-51	0.47415783170623627
1103	52-53	0.4330497959600379
1103	54-55	0.43136193282719404
1103	56-57	0.5020234458761408
1103	58-59	0.4243666555040164
1103	60-61	0.40847048195060154
1103	62-63	0.3916465384429202
1103	64-65	0.4059942314474725
1103	66-67	0.4177588835990207
1103	68-69	0.4075689416284334
1103	70-71	0.39858109995757474
1103	72-73	0.43268351612939426
1103	74-75	0.42728023843331187
1103	76-77	0.3854004752089111
1103	78-79	0.38700679680673744
1103	80-81	0.3619208983430866
1103	82-83	0.4042943439266651
1103	84-85	0.3897310750800429
1103	86-87	0.43252754273250105
1103	88-89	0.43087471846478564
1103	90-91	0.389440227136312
1103	92-93	0.4272377803316516
1103	94-95	0.42827768559208934
1103	96-97	0.44422089806670684
1103	98-99	0.470344266631443
1103	100	0.47330706483583995
1104	1	0.27113797076314583
1104	2	0.22332902119561737
1104	3	0.33883233213384045
1104	4	0.27797553525360996
1104	5	0.20151940338929109
1104	6	0.2239989456361826
1104	7	0.170213153303564
1104	8	0.21243920901969915
1104	9	0.2352129521489772
1104	10-11	0.23815835113621375
1104	12-13	0.2737259393652067
1104	14-15	0.34313582735215675
1104	16-17	0.3441802250297812
1104	18-19	0.3709189413043248
1104	20-21	0.35967904556439123
1104	22-23	0.3573888740405451
1104	24-25	0.35545661403941864
1104	26-27	0.38732224938284787
1104	28-29	0.36914814070900803
1104	30-31	0.3983123661751762
1104	32-33	0.3482010383548797
1104	34-35	0.3551374061313268
1104	36-37	0.3886506906948455
1104	38-39	0.42859816816986296
1104	40-41	0.43580690286837154
1104	42-43	0.3798666415064602
1104	44-45	0.43586396524917603
1104	46-47	0.5011122905349055
1104	48-49	0.4906986873423449
1104	50-51	0.5395381788690656
1104	52-53	0.5264422514752454
1104	54-55	0.4662689898313417
1104	56-57	0.48716237648008587
1104	58-59	0.4988218527079553
1104	60-61	0.4813268532778565
1104	62-63	0.5265968432030519
1104	64-65	0.519378765039086
1104	66-67	0.47887514661547215
1104	68-69	0.49962424490845336
1104	70-71	0.515789944929999
1104	72-73	0.5378661271052181
1104	74-75	0.5229261953724205
1104	76-77	0.48102128480215356
1104	78-79	0.45103565250514777
1104	80-81	0.4820784079503113
1104	82-83	0.4656946794871999
1104	84-85	0.4967638179363689
1104	86-87	0.5606488869616726
1104	88-89	0.4748821696510319
1104	90-91	0.468462962106166
1104	92-93	0.5405777782212766
1104	94-95	0.5293370310057988
1104	96-97	0.5193266900166584
1104	98-99	0.5853557621501437
1104	100	0.652821422408632
1105	1	0.3110505012607412
1105	2	0.2789906317996227
1105	3	0.36863044383191124
1105	4	0.27249474481320135
1105	5	0.2035737101291133
1105	6	0.22915888508669013
1105	7	0.21881443062303418
1105	8	0.20099540226637203
1105	9	0.25017923235157724
1105	10-11	0.22768181276141064
1105	12-13	0.20828595115415283
1105	14-15	0.30485815641883107
1105	16-17	0.259723269796865
1105	18-19	0.3194216182342018
1105	20-21	0.30225577784268864
1105	22-23	0.30467833163218216
1105	24-25	0.2979341020260904
1105	26-27	0.3234477243516807
1105	28-29	0.3533719557600463
1105	30-31	0.34079907478296434
1105	32-33	0.3622265769810298
1105	34-35	0.33927773112066717
1105	36-37	0.3764277519671495
1105	38-39	0.3969564717532563
1105	40-41	0.38453994818854653
1105	42-43	0.4224373488515525
1105	44-45	0.47299642242825257
1105	46-47	0.5220669472617558
1105	48-49	0.5339091488460568
1105	50-51	0.5672569213943888
1105	52-53	0.5033623852157234
1105	54-55	0.5001033373372437
1105	56-57	0.47646135171455484
1105	58-59	0.46705782191091316
1105	60-61	0.5024373637251358
1105	62-63	0.49559623218976157
1105	64-65	0.5201242725752167
1105	66-67	0.5734183648227145
1105	68-69	0.5663411349997816
1105	70-71	0.5339072637970332
1105	72-73	0.507396742278857
1105	74-75	0.5772331103604103
1105	76-77	0.5459993731451505
1105	78-79	0.5177384839519021
1105	80-81	0.5018851498975181
1105	82-83	0.483181391247939
1105	84-85	0.4494578066673398
1105	86-87	0.5154632051625327
1105	88-89	0.46209559243148846
1105	90-91	0.42124095588034294
1105	92-93	0.4388398270682359
1105	94-95	0.47776709554440444
1105	96-97	0.49289177885055224
1105	98-99	0.5314887849961067
1105	100	0.535715932883825
1106	1	0.179187806724034
1106	2	0.19924130521403782
1106	3	0.2867806078576436
1106	4	0.23403498667503442
1106	5	0.17045857777992524
1106	6	0.1863327684500291
1106	7	0.16640004030776367
1106	8	0.19550270594460528
1106	9	0.19890286946179003
1106	10-11	0.19622979993477685
1106	12-13	0.16667286742621457
1106	14-15	0.2731764808445476
1106	16-17	0.2722376671097777
1106	18-19	0.24549137025846335
1106	20-21	0.1708274181640661
1106	22-23	0.1605891524693348
1106	24-25	0.2242927403111068
1106	26-27	0.2085684003251913
1106	28-29	0.22966815706636368
1106	30-31	0.2535878910471041
1106	32-33	0.22008320965663586
1106	34-35	0.2133373039655524
1106	36-37	0.2436351364815721
1106	38-39	0.2634733808065022
1106	40-41	0.287957607845847
1106	42-43	0.292351739066099
1106	44-45	0.2877503293101853
1106	46-47	0.2760540147614208
1106	48-49	0.2803640167959216
1106	50-51	0.32158114428191453
1106	52-53	0.2825789412268733
1106	54-55	0.2645875836947553
1106	56-57	0.31886493119563397
1106	58-59	0.27495027472369316
1106	60-61	0.31461188702873244
1106	62-63	0.2766162165215391
1106	64-65	0.2924155785634923
1106	66-67	0.2905493217644377
1106	68-69	0.2801558303512266
1106	70-71	0.272026626224509
1106	72-73	0.27492525715322813
1106	74-75	0.28609604052887505
1106	76-77	0.29059228754883293
1106	78-79	0.27944406620628826
1106	80-81	0.1973704146939923
1106	82-83	0.25857750191249806
1106	84-85	0.2289205896126134
1106	86-87	0.30124001159247626
1106	88-89	0.274377514375054
1106	90-91	0.25987989930467137
1106	92-93	0.2735774412901151
1106	94-95	0.26755629246926205
1106	96-97	0.25756554985001756
1106	98-99	0.31320326665678877
1106	100	0.30544358019231765
1107	1	0.1506271297010322
1107	2	0.1676519651156454
1107	3	0.2250314022634825
1107	4	0.21676663136764773
1107	5	0.1822668502709135
1107	6	0.1718008756455376
1107	7	0.1454043389909785
1107	8	0.1535725270956263
1107	9	0.13659437967104537
1107	10-11	0.1690300558341562
1107	12-13	0.15310617048832853
1107	14-15	0.17705243557393402
1107	16-17	0.16934815231978462
1107	18-19	0.17791333686973587
1107	20-21	0.20195234908267423
1107	22-23	0.20115833643513525
1107	24-25	0.20717075922188144
1107	26-27	0.19143409556055957
1107	28-29	0.20342808779967214
1107	30-31	0.18075709891282088
1107	32-33	0.1941600828849701
1107	34-35	0.16472582118026935
1107	36-37	0.21788074851285444
1107	38-39	0.1893429447563193
1107	40-41	0.18152010723358103
1107	42-43	0.18606344004076192
1107	44-45	0.2157817183343056
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2216	88-89	-1.353457358172978
2216	90-91	-1.1878123641543397
2216	92-93	-1.2402184918977142
2216	94-95	-1.2798177231346095
2216	96-97	-1.341919200493951
2216	98-99	-1.3592090356953506
2216	100	-1.35882026762539
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	562.0
3	46.0
4	75.0
5	172.0
6	413.0
7	1116.0
8	2570.0
9	4197.0
10	5679.0
11	7917.0
12	11306.0
13	15924.0
14	19085.0
15	24939.0
16	33555.0
17	48328.0
18	49849.0
19	27769.0
20	21110.0
21	20506.0
22	22001.0
23	24203.0
24	28059.0
25	34330.0
26	43726.0
27	57580.0
28	75472.0
29	99113.0
30	129159.0
31	166938.0
32	214370.0
33	280430.0
34	382486.0
35	556503.0
36	924211.0
37	1691419.0
38	2297058.0
39	814983.0
40	1871.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.685029825277475	20.074624663758723	14.86579854537229	23.374546965591513
2	15.564893294827446	20.38070945931418	39.6660775290376	24.38831971682077
3	18.200205454483577	25.034325347655106	30.654479791139703	26.110989406721615
4	12.994764354110835	17.21505512054336	37.15158327791633	32.63859724742947
5	13.10231069734982	37.48687934526658	35.0731334820433	14.337676475340293
6	31.00241113782508	37.4751440490209	17.095524995036183	14.42691981811784
7	27.0810526561519	32.896475378515625	21.774855234591836	18.24761673074064
8	25.462862282114703	36.000174467964854	19.74501875530622	18.791944494614224
9	25.10851115459161	16.768384419509708	21.21077156047561	36.91233286542307
10-11	24.26923724325872	26.875076022572713	28.072675736543545	20.783010997625023
12-13	24.906939144706328	24.318625723043866	28.641862561246555	22.132572571003248
14-15	22.094604639619217	26.1833965472544	26.42519440277281	25.29680441035357
16-17	21.18451461660665	29.577234633611134	27.180487109643288	22.057763640138937
18-19	21.13617347521756	27.856710197652546	30.028609060293427	20.978507266836466
20-21	21.99389239258241	26.94304481000807	30.025992040820586	21.03707075658893
22-23	21.7995879115816	26.721224765113288	30.098770983765878	21.38041633953923
24-25	21.661997805635316	26.864577228490372	29.977288448377752	21.496136517496556
26-27	21.42778684929285	26.97762509783107	29.997868296344897	21.596719756531183
28-29	21.47362686495096	26.918489336739636	30.205887169967987	21.40199662834142
30-31	21.657173801596816	27.070296961883866	29.913069867548348	21.35945936897097
32-33	21.514152456893353	26.992705579050373	30.066223505667782	21.426918458388492
34-35	21.681127849387458	26.84719800762499	29.979112990122903	21.492561152864653
36-37	21.822520619779016	26.874559990563984	29.72299524636228	21.57992414329472
38-39	21.653354269489117	27.026457698276086	29.817625687581934	21.502562344652866
40-41	21.619038140908682	26.9277235739394	29.796271791601704	21.656966493550215
42-43	21.63797160103845	27.024590153863542	29.669874665654262	21.66756357944374
44-45	21.72495985393837	27.091600596139838	29.47009041617982	21.713349133741982
46-47	21.892559678487487	27.17621755909488	29.09098504367228	21.840237718745353
48-49	21.982650266677968	27.241176159812657	28.784732332968428	21.991441240540947
50-51	21.925757737715674	27.359458068099023	28.614785791427234	22.099998402758068
52-53	21.919405629417756	27.489590282871546	28.59093774073815	22.000066346972552
54-55	21.9442857441243	27.63832422291108	28.50079800664207	21.916592026322547
56-57	21.91088495803554	27.458726654159033	28.49926834033048	22.131120047474944
58-59	21.811263958619058	27.203779328266176	28.750895762845925	22.234060950268844
60-61	21.956050045275667	27.327605378036452	28.628970528429065	22.087374048258823
62-63	21.744132900357904	27.394069072113012	28.690587207566505	22.171210819962575
64-65	21.73099251384993	27.286249098479793	28.743885942182303	22.238872445487974
66-67	21.72459125964642	27.23201044841953	28.727483496190576	22.315914795743474
68-69	21.84443489239104	27.39850003000972	28.52547714687605	22.23158793072319
70-71	21.829493195135047	27.21304627209876	28.54816237315749	22.4092981596087
72-73	21.755553180170118	27.302731406568103	28.53389777405907	22.407817639202708
74-75	21.780795745930412	27.393688191344665	28.38438978600644	22.441126276718478
76-77	21.812777443995166	27.305815312144077	28.283210652866497	22.598196590994256
78-79	21.764111541431795	27.30954541765806	28.41047027710521	22.515872763804932
80-81	21.695924873312116	27.327428552788156	28.363514257927434	22.61313231597229
82-83	21.63309008495632	27.29297041923793	28.46248669677845	22.6114527990273
84-85	21.65367986111367	27.25115892188627	28.290852841186236	22.804308375813825
86-87	21.704203185404353	27.227791716444816	28.299602550893816	22.768402547257015
88-89	21.627733980224782	27.134184905283202	28.353587495998443	22.88449361849356
90-91	21.50791006762295	27.161970783606616	28.297659431382854	23.032459717387578
92-93	21.534404799327582	27.16440224310842	28.281478002109235	23.019714955454766
94-95	21.444064332635314	26.995681706779084	28.391034772848368	23.169219187737234
96-97	21.453761778354533	27.020199398466367	28.315409103751254	23.21062971942785
98-99	21.375653126168558	27.025516620318996	28.269319858014995	23.32951039549745
100	21.385968499107502	26.929083183272915	28.22398089973343	23.460967417886152
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	724.0
1	615.5
2	1147.5
3	1960.5
4	1452.5
5	908.5
6	1925.0
7	5211.0
8	8949.0
9	10366.0
10	8669.5
11	6246.5
12	5760.5
13	6251.5
14	6932.5
15	7663.0
16	8380.5
17	9161.0
18	10146.5
19	11080.5
20	12280.0
21	14212.5
22	17263.5
23	22195.0
24	28336.5
25	35553.5
26	45998.0
27	60033.0
28	78549.5
29	99133.5
30	117493.5
31	135678.5
32	153938.5
33	171126.5
34	189354.0
35	209188.5
36	226808.5
37	244911.5
38	264307.0
39	277095.0
40	287334.0
41	295336.5
42	297798.5
43	301946.0
44	303427.0
45	300819.0
46	297267.5
47	288699.0
48	278714.5
49	272535.5
50	264718.0
51	252725.5
52	237635.0
53	226174.5
54	217973.5
55	204719.5
56	187053.5
57	167089.0
58	151540.5
59	140262.5
60	124532.0
61	105104.5
62	89181.5
63	74512.0
64	59950.5
65	48287.5
66	38539.5
67	29800.5
68	22576.5
69	17163.5
70	12518.0
71	8677.0
72	6285.5
73	4334.5
74	2785.5
75	1816.5
76	1098.5
77	630.5
78	373.5
79	204.5
80	108.0
81	59.0
82	34.0
83	15.5
84	7.0
85	4.5
86	3.5
87	3.0
88	1.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	1.0
97	1.0
98	0.0
99	0.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06130951722748289
2	1.8429714597439743E-4
3	1.2286476398293162E-5
4	0.1198668637417481
5	0.0017815390777525086
6	0.0016463878373712837
7	0.0
8	0.0
9	6.143238199146582E-5
10-11	0.0
12-13	1.2286476398293162E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.1057828758463846E-4
30-31	2.7644571896159615E-4
32-33	1.8429714597439743E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.1501333697013036E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	6.143238199146581E-6
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.1672152578378505E-4
80-81	3.870240065462346E-4
82-83	2.948754335590359E-4
84-85	0.0
86-87	6.143238199146581E-6
88-89	4.300266739402607E-5
90-91	4.300266739402607E-5
92-93	6.941859165035636E-4
94-95	6.204670581138047E-4
96-97	6.143238199146581E-6
98-99	1.8429714597439743E-5
100	0.0010566369702532119
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8139030.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.89528464621336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.66334714956896	35.25767820081933
2	15.653050984951946	15.620268689517355
3	5.658343885841792	8.469740364307118
4	2.538091735984052	5.065552385005043
5	1.4329128132752353	3.5747796345787117
6	0.8730949832967168	2.613799362886235
7	0.5975751944739447	2.0871329098055647
8	0.41982267772630205	1.675773760487147
9	0.32398986281542885	1.4549009784897098
>10	1.6886067217102105	15.03023393264077
>50	0.10490267914534501	3.5481459658744994
>100	0.04433803988623544	3.849853996483487
>500	0.0013316237964289082	0.44763250941537147
>1k	4.930396189760687E-4	0.4230030295182735
>5k	2.4651977097156194E-5	0.08810614739021576
>10k+	7.395593129146859E-5	0.7933981327811096
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25578	0.3142634933155425	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24132	0.2964972484436106	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14793	0.18175384535995076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.2286476398293163E-5	0.0	0.0	0.0	0.0
6	1.2286476398293163E-5	0.0	0.0	0.0	0.0
7	1.2286476398293163E-5	0.0	0.0	0.0	0.0
8	1.2286476398293163E-5	0.0	0.0	0.0	0.0
9	1.2286476398293163E-5	0.0	0.0	0.0	0.0
10-11	1.2286476398293163E-5	0.0	0.0	0.0	0.0
12-13	1.2286476398293163E-5	0.0	0.0	6.143238199146582E-6	0.0
14-15	1.2286476398293163E-5	1.8429714597439743E-5	0.0	1.2286476398293163E-5	0.0
16-17	1.2286476398293163E-5	3.685942919487949E-5	0.0	2.4572952796586327E-5	0.0
18-19	1.2286476398293163E-5	3.685942919487949E-5	0.0	3.685942919487949E-5	0.0
20-21	1.2286476398293163E-5	3.685942919487949E-5	0.0	6.75756201906124E-5	0.0
22-23	1.2286476398293163E-5	3.685942919487949E-5	0.0	1.4743771677951795E-4	0.0
24-25	1.2286476398293163E-5	4.9145905593172653E-5	0.0	2.8258895716074277E-4	0.0
26-27	1.2286476398293163E-5	6.143238199146582E-5	0.0	6.696129637069774E-4	0.0
28-29	1.2286476398293163E-5	6.143238199146582E-5	0.0	0.0021378468933030102	0.0
30-31	1.2286476398293163E-5	6.143238199146582E-5	0.0	0.0073043102187852855	0.0
32-33	1.2286476398293163E-5	6.143238199146582E-5	0.0	0.01829456335705852	0.0
34-35	1.2286476398293163E-5	6.75756201906124E-5	0.0	0.03638025661534606	1.2286476398293163E-5
36-37	1.2286476398293163E-5	7.371885838975897E-5	0.0	0.06524733291313584	1.2286476398293163E-5
38-39	1.2286476398293163E-5	7.371885838975897E-5	0.0	0.10627187760703671	1.2286476398293163E-5
40-41	1.2286476398293163E-5	7.371885838975897E-5	0.0	0.149157823475279	1.2286476398293163E-5
42-43	1.2286476398293163E-5	8.600533478805214E-5	0.0	0.19282396059481288	1.2286476398293163E-5
44-45	1.2286476398293163E-5	8.600533478805214E-5	0.0	0.25022637832763855	1.2286476398293163E-5
46-47	1.2286476398293163E-5	8.600533478805214E-5	0.0	0.3219241113498783	1.2286476398293163E-5
48-49	1.2286476398293163E-5	8.600533478805214E-5	0.0	0.3999370932408407	1.2286476398293163E-5
50-51	1.2286476398293163E-5	9.829181118634531E-5	0.0	0.48071453232141914	1.2286476398293163E-5
52-53	1.2286476398293163E-5	1.1672152578378505E-4	0.0	0.5621923005567002	1.2286476398293163E-5
54-55	1.2286476398293163E-5	1.2286476398293164E-4	0.0	0.6580575326543827	1.2286476398293163E-5
56-57	1.2286476398293163E-5	1.2286476398293164E-4	0.0	0.7753749525434849	1.2286476398293163E-5
58-59	1.2286476398293163E-5	1.2286476398293164E-4	0.0	0.9087262241323597	1.2286476398293163E-5
60-61	1.2286476398293163E-5	1.351512403812248E-4	0.0	1.0484971796393427	1.2286476398293163E-5
62-63	1.2286476398293163E-5	1.351512403812248E-4	0.0	1.1911861732909204	1.2286476398293163E-5
64-65	1.2286476398293163E-5	1.351512403812248E-4	0.0	1.349546567588521	1.2286476398293163E-5
66-67	1.2286476398293163E-5	1.351512403812248E-4	0.0	1.5389118850772143	1.2286476398293163E-5
68-69	1.2286476398293163E-5	1.351512403812248E-4	0.0	1.7527764365041043	1.2286476398293163E-5
70-71	1.2286476398293163E-5	1.4743771677951795E-4	0.0	1.9842843680389433	1.2286476398293163E-5
72-73	1.2286476398293163E-5	1.7201066957610428E-4	0.0	2.2265859690896828	1.2286476398293163E-5
74-75	1.2286476398293163E-5	1.7201066957610428E-4	0.0	2.4780717112481465	1.2286476398293163E-5
76-77	1.2286476398293163E-5	1.7201066957610428E-4	0.0	2.7601692093529575	1.2286476398293163E-5
78-79	1.2286476398293163E-5	1.7201066957610428E-4	0.0	3.079261287893029	1.2286476398293163E-5
80-81	1.2286476398293163E-5	1.7201066957610428E-4	0.0	3.4281480716989616	1.2286476398293163E-5
82-83	1.2286476398293163E-5	1.7815390777525087E-4	0.0	3.793412728543819	1.2286476398293163E-5
84-85	2.4572952796586327E-5	1.8429714597439743E-4	0.0	4.165545525695322	1.2286476398293163E-5
86-87	2.4572952796586327E-5	1.8429714597439743E-4	0.0	4.565206173217202	1.2286476398293163E-5
88	2.4572952796586327E-5	1.8429714597439743E-4	0.0	4.890828513962966	1.2286476398293163E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15105	0.0	55.48516	1
GTATCAA	26585	0.0	41.478954	1
ATCAACG	33790	0.0	32.194622	3
TCAACGC	34070	0.0	31.888597	4
GTGGTAT	6010	0.0	31.337408	1
TATCAAC	35245	0.0	31.026129	2
CAACGCA	35265	0.0	30.945494	5
AACGCAG	37045	0.0	29.598127	6
ACGCAGA	42335	0.0	25.732677	7
CGCAGAG	42830	0.0	25.171925	8
TGGTATC	7325	0.0	24.60255	2
GTACATG	25685	0.0	23.904276	1
GCAGAGT	46185	0.0	22.936337	9
TACATGG	26125	0.0	22.585527	2
ACATGGG	26810	0.0	21.253525	3
GAGTACT	30520	0.0	21.188734	12-13
AGAGTAC	42530	0.0	20.526009	10-11
CAGAGTA	44380	0.0	20.48578	10-11
AGTACTT	31535	0.0	20.037292	12-13
GTACTTT	32795	0.0	19.539732	14-15
>>END_MODULE