FastQCFastQC Report
Wed 25 May 2016
SRR2049524_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049524_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10087697
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94550.043.630131
GTATCAA148650.035.6359561
TCAACGC149650.033.887674
ATCAACG152050.033.352783
CAACGCA164950.030.8371355
TATCAAC179450.028.7451322
AACGCAG181400.028.040246
ACGCAGA206300.024.3073467
CGCAGAG213750.023.2385358
GTACATG248200.022.5182081
TACATGG250800.021.1795652
ACATGGG258250.019.9343663
GTGGTAT57900.018.740031
GCAGAGT245650.018.7106749
GAGTACA195300.017.0342921
AGAGTAC211750.016.3651510-11
CATGGGG182000.016.279914
CAGAGTA238600.016.14465910-11
GTATAGG32050.015.649881
CCTATAC28300.014.4659963
Produced by FastQC (version 0.11.5)