##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049524_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10087697 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.917485428041704 31.0 31.0 34.0 28.0 34.0 2 31.10142770941673 31.0 31.0 34.0 28.0 34.0 3 31.19374313086525 31.0 31.0 34.0 28.0 34.0 4 34.84823513235975 37.0 35.0 37.0 32.0 37.0 5 34.880705477176804 37.0 35.0 37.0 32.0 37.0 6 34.96605835801769 37.0 35.0 37.0 32.0 37.0 7 31.010932624165854 37.0 35.0 37.0 2.0 37.0 8 32.77784959242927 37.0 35.0 37.0 17.0 37.0 9 35.64959425327704 39.0 35.0 39.0 30.0 39.0 10-11 36.20665549332023 39.0 36.0 39.0 32.0 39.0 12-13 36.37204933891254 39.0 37.0 39.0 32.0 39.0 14-15 37.6009028126043 40.0 38.0 41.0 32.0 41.0 16-17 37.61537127849895 40.0 38.0 41.0 32.0 41.0 18-19 37.61497237674764 40.0 38.0 41.0 32.0 41.0 20-21 37.68598134936052 40.0 38.0 41.0 32.0 41.0 22-23 37.64850907000874 40.0 38.0 41.0 32.0 41.0 24-25 37.628132466706724 40.0 38.0 41.0 32.0 41.0 26-27 37.65728024939686 40.0 38.0 41.0 32.0 41.0 28-29 37.64695995528018 40.0 38.0 41.0 32.0 41.0 30-31 37.57881323160281 40.0 38.0 41.0 32.0 41.0 32-33 37.53837070046811 40.0 38.0 41.0 32.0 41.0 34-35 37.489220631825084 40.0 38.0 41.0 32.0 41.0 36-37 37.325000691436315 40.0 37.0 41.0 31.0 41.0 38-39 37.227700435490874 40.0 37.0 41.0 31.0 41.0 40-41 37.16548970493463 40.0 37.0 41.0 30.5 41.0 42-43 37.06250965904309 40.0 37.0 41.0 30.0 41.0 44-45 36.95553866259068 40.0 37.0 41.0 30.0 41.0 46-47 36.81471157391028 40.0 37.0 41.0 30.0 41.0 48-49 36.74319767931174 40.0 36.0 41.0 30.0 41.0 50-51 36.61446175474937 40.0 36.0 41.0 30.0 41.0 52-53 36.50855666065307 40.0 36.0 41.0 30.0 41.0 54-55 36.40059802549581 39.5 36.0 41.0 30.0 41.0 56-57 36.21314136417857 39.0 35.0 41.0 30.0 41.0 58-59 36.04596856943661 39.0 35.0 41.0 29.0 41.0 60-61 35.83903937638095 39.0 35.0 41.0 29.0 41.0 62-63 35.61679400164378 39.0 35.0 40.5 29.0 41.0 64-65 35.26873631315453 38.0 34.5 40.0 28.0 41.0 66-67 31.71226207527843 37.0 31.0 40.0 7.0 41.0 68-69 32.09546787537334 36.0 31.0 40.0 11.0 41.0 70-71 32.26511130340255 36.0 31.0 39.0 15.5 41.0 72-73 33.53692611901408 36.0 32.5 39.0 26.0 41.0 74-75 33.61919271564164 36.0 33.5 39.0 26.0 40.5 76-77 33.31253050126307 35.0 33.5 37.5 26.0 40.0 78-79 29.869566512554847 35.0 30.0 37.0 6.5 39.0 80-81 31.694609185823087 35.0 30.5 36.5 22.5 39.0 82-83 32.09825161283096 35.0 32.5 36.0 25.0 38.5 84-85 30.2172972185822 35.0 31.0 36.0 13.5 37.0 86-87 29.84859264706305 35.0 29.0 35.5 14.5 37.0 88-89 31.114756371052778 35.0 31.0 35.0 23.5 36.5 90-91 31.269033705116243 35.0 32.5 35.0 24.0 36.0 92-93 31.12320215406946 35.0 32.0 35.0 23.0 36.0 94-95 30.922450535538488 35.0 32.0 35.0 21.0 36.0 96-97 30.741160296547367 35.0 32.0 35.0 19.5 35.5 98-99 30.565546824017414 35.0 32.0 35.0 10.0 35.0 100 30.367603626476885 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.30500014568521294 1101 2 0.3185134491542918 1101 3 0.32506344919911356 1101 4 0.3797556948867964 1101 5 0.3656994628628354 1101 6 0.41177127687895165 1101 7 -12.223228115100557 1101 8 -6.602028575678762 1101 9 -2.1966809335314395 1101 10-11 -0.07512439002719162 1101 12-13 0.4063480913376125 1101 14-15 0.5717282667733627 1101 16-17 0.6602022179645033 1101 18-19 0.6292426725598048 1101 20-21 0.6055498394755361 1101 22-23 0.5768892661970071 1101 24-25 0.6156829431052202 1101 26-27 0.596033429082965 1101 28-29 0.5352655601805196 1101 30-31 0.6078228694880536 1101 32-33 0.6567416796130274 1101 34-35 0.62416425447676 1101 36-37 0.6532939996472891 1101 38-39 0.6524978665715153 1101 40-41 0.617747210343758 1101 42-43 0.6234521546973895 1101 44-45 0.5302355414168431 1101 46-47 0.5305980803692592 1101 48-49 0.497349867811522 1101 50-51 0.603463870646344 1101 52-53 0.6174108421008668 1101 54-55 0.5100169495190201 1101 56-57 0.5224058366631468 1101 58-59 0.4588216462588477 1101 60-61 0.4739779285236736 1101 62-63 0.5271799982713077 1101 64-65 0.41538829540128575 1101 66-67 -10.47817066395777 1101 68-69 -8.293178540841058 1101 70-71 -6.759663374513472 1101 72-73 -1.4843652988125484 1101 74-75 -0.19559350388919228 1101 76-77 0.0794329732948853 1101 78-79 3.278325172871437 1101 80-81 1.1421228745684466 1101 82-83 0.420780806910777 1101 84-85 -5.451134088345924 1101 86-87 -5.926241857524047 1101 88-89 -1.2546465914872265 1101 90-91 0.02671495342036323 1101 92-93 0.35949903752475976 1101 94-95 0.40947707474347084 1101 96-97 0.5404595622421873 1101 98-99 0.5521692190899543 1101 100 0.5705170494835166 1102 1 0.21500565340688027 1102 2 0.23827620424295048 1102 3 0.2099068820048764 1102 4 0.2884971596136907 1102 5 0.2737715826944722 1102 6 0.34479053676297156 1102 7 -12.34464246503562 1102 8 -6.707095570457792 1102 9 -2.305785950541015 1102 10-11 -0.19607910197642298 1102 12-13 0.36100264425090955 1102 14-15 0.5580626802540678 1102 16-17 0.5733752644988854 1102 18-19 0.5592558335615792 1102 20-21 0.5787499215824639 1102 22-23 0.564663070123224 1102 24-25 0.5948344710671662 1102 26-27 0.5666777516607482 1102 28-29 0.535564938252584 1102 30-31 0.5486320640003584 1102 32-33 0.569261914120105 1102 34-35 0.5804063998484139 1102 36-37 0.6714469092344544 1102 38-39 0.6691687636107133 1102 40-41 0.5802269038228189 1102 42-43 0.5724753697144109 1102 44-45 0.5696526553098451 1102 46-47 0.5786897194460181 1102 48-49 0.4518038482059836 1102 50-51 0.5507658910563009 1102 52-53 0.5295591894149396 1102 54-55 0.47348583559143975 1102 56-57 0.5353597012845697 1102 58-59 0.48549791002414366 1102 60-61 0.4772231336609636 1102 62-63 0.43348414985713646 1102 64-65 0.3253441666171284 1102 66-67 -10.518997907924795 1102 68-69 -6.851035861751136 1102 70-71 -6.401259916602616 1102 72-73 -1.0522854787831335 1102 74-75 -0.08965419123381224 1102 76-77 0.10367533004870921 1102 78-79 3.314143177686649 1102 80-81 1.1279044161093275 1102 82-83 0.48825874087626886 1102 84-85 -5.57033959861057 1102 86-87 -6.072092769189798 1102 88-89 -1.3548290372311946 1102 90-91 -0.038485616521136023 1102 92-93 0.3305299785533684 1102 94-95 0.3190251427973756 1102 96-97 0.43118499426651624 1102 98-99 0.427754738579857 1102 100 0.523588371199363 1103 1 0.18520066665927715 1103 2 0.2103062821103805 1103 3 0.24768606266593807 1103 4 0.2430230239192852 1103 5 0.2621307671886228 1103 6 0.3165766260594651 1103 7 -12.305229894431367 1103 8 -6.708476536106385 1103 9 -2.337505933525783 1103 10-11 -0.2947318077169072 1103 12-13 0.26181437635003846 1103 14-15 0.4526863693856171 1103 16-17 0.46545148587137675 1103 18-19 0.5065585152694325 1103 20-21 0.4622981722887687 1103 22-23 0.46869554315640016 1103 24-25 0.5551455652883632 1103 26-27 0.5055662048595906 1103 28-29 0.47707178499705805 1103 30-31 0.4533901203918518 1103 32-33 0.48836123702621137 1103 34-35 0.44862867032507836 1103 36-37 0.5129276748085516 1103 38-39 0.5814098238549832 1103 40-41 0.554636474188996 1103 42-43 0.54222571017268 1103 44-45 0.4881378758930026 1103 46-47 0.4756235020301176 1103 48-49 0.42028444315234736 1103 50-51 0.44017782970599484 1103 52-53 0.4239240332457399 1103 54-55 0.39254429886695874 1103 56-57 0.3763339417539484 1103 58-59 0.37205895576167336 1103 60-61 0.36042939452558187 1103 62-63 0.2717962431076586 1103 64-65 0.17639966214129288 1103 66-67 -10.639638134128191 1103 68-69 -8.530253511480446 1103 70-71 -6.97864889759947 1103 72-73 -1.676603723985636 1103 74-75 -0.45883242342578257 1103 76-77 -0.17446117105313874 1103 78-79 3.002300447868695 1103 80-81 0.8285678640336762 1103 82-83 0.18500231190304106 1103 84-85 -5.720335283153311 1103 86-87 -6.236308257421946 1103 88-89 -1.5923757252897452 1103 90-91 -0.30471276008248793 1103 92-93 0.09122030155307215 1103 94-95 0.16105390187034274 1103 96-97 0.23005881839379683 1103 98-99 0.24299656256149404 1103 100 0.3152709293328506 1104 1 0.13067354138863507 1104 2 0.12284609645021405 1104 3 0.16068450043071536 1104 4 0.23741033296159486 1104 5 0.21415372094453744 1104 6 0.2591585341204734 1104 7 -12.230139417471193 1104 8 -6.657314080384051 1104 9 -2.346549801008692 1104 10-11 -0.27673116960333743 1104 12-13 0.3368464175460204 1104 14-15 0.4932941821373049 1104 16-17 0.4731806245865542 1104 18-19 0.4761625697350027 1104 20-21 0.46339727681394294 1104 22-23 0.5116075168814618 1104 24-25 0.5692316675315965 1104 26-27 0.5151824359045634 1104 28-29 0.5071857948801792 1104 30-31 0.4763383658892053 1104 32-33 0.4642405397430096 1104 34-35 0.47197622457690613 1104 36-37 0.5497590527165102 1104 38-39 0.5675914659175305 1104 40-41 0.5931330113856532 1104 42-43 0.4776579594605437 1104 44-45 0.46621774598200005 1104 46-47 0.48621805700962284 1104 48-49 0.4181853802532771 1104 50-51 0.45051304750951005 1104 52-53 0.4395298104780778 1104 54-55 0.4640231405141222 1104 56-57 0.5031372814469108 1104 58-59 0.4381696402683417 1104 60-61 0.4034170416219425 1104 62-63 0.34714277860125264 1104 64-65 0.23121401260658558 1104 66-67 -10.506361748553825 1104 68-69 -8.3891951515783 1104 70-71 -6.831301100401383 1104 72-73 -1.5940321301430131 1104 74-75 -0.36688779328873977 1104 76-77 -0.09679892226031939 1104 78-79 3.0800062232300185 1104 80-81 0.967640335176629 1104 82-83 0.2921857436856925 1104 84-85 -5.549461214622568 1104 86-87 -6.045751031939329 1104 88-89 -1.4144864223311835 1104 90-91 -0.10159675291013315 1104 92-93 0.2040393908970053 1104 94-95 0.282929356652577 1104 96-97 0.3935058158764342 1104 98-99 0.4150922878254377 1104 100 0.4638953770288303 1105 1 0.056442704751269446 1105 2 0.09391135504682069 1105 3 0.09800220572004292 1105 4 0.12716230228913616 1105 5 0.11814484439820916 1105 6 0.18995343486149352 1105 7 -12.288944485675565 1105 8 -6.738470573708167 1105 9 -2.4837390113218447 1105 10-11 -0.38678436305499986 1105 12-13 0.15816890696089558 1105 14-15 0.3022387738691066 1105 16-17 0.3881383734406043 1105 18-19 0.3606741046796671 1105 20-21 0.3908679015054659 1105 22-23 0.36343803727896784 1105 24-25 0.4049327802109488 1105 26-27 0.37230684737976105 1105 28-29 0.3589870073339583 1105 30-31 0.40603971980743125 1105 32-33 0.3687344980020981 1105 34-35 0.40082826971660523 1105 36-37 0.49128569573522185 1105 38-39 0.4172091122459918 1105 40-41 0.4531127251915663 1105 42-43 0.3719920532982428 1105 44-45 0.321961638313752 1105 46-47 0.331515128139074 1105 48-49 0.28286269435396605 1105 50-51 0.3825908156875286 1105 52-53 0.3642142846679235 1105 54-55 0.3667393839555402 1105 56-57 0.3433069076202102 1105 58-59 0.31780308087454756 1105 60-61 0.2862765782201464 1105 62-63 0.2143609639969668 1105 64-65 0.10349436052388228 1105 66-67 -10.592357813208704 1105 68-69 -8.535600078520247 1105 70-71 -6.9520291296143455 1105 72-73 -1.762868869263496 1105 74-75 -0.5195755135431384 1105 76-77 -0.2266583650295928 1105 78-79 2.9503217423580885 1105 80-81 0.816170401941239 1105 82-83 0.14164901826814713 1105 84-85 -5.690502135194091 1105 86-87 -6.165634896026674 1105 88-89 -1.5459142596625846 1105 90-91 -0.2875404424358905 1105 92-93 0.08323818506315561 1105 94-95 0.16473511261462548 1105 96-97 0.22925988797900843 1105 98-99 0.24369747492122684 1105 100 0.2851051411924992 1106 1 0.17571387957278262 1106 2 0.1676645315965999 1106 3 0.16388230168522 1106 4 0.17082600938333314 1106 5 0.1693309199336781 1106 6 0.20704449032839278 1106 7 -12.296339064249597 1106 8 -6.756018514689028 1106 9 -2.507050424855322 1106 10-11 -0.364150143594415 1106 12-13 0.23073747708678383 1106 14-15 0.3424153685798146 1106 16-17 0.3492069315989923 1106 18-19 0.37135566204413095 1106 20-21 0.3412119494242418 1106 22-23 0.3665542065296208 1106 24-25 0.43733284888382684 1106 26-27 0.4006662170851314 1106 28-29 0.3862528434668988 1106 30-31 0.3888793911398025 1106 32-33 0.4153242819096121 1106 34-35 0.4627578022475376 1106 36-37 0.5148414454944827 1106 38-39 0.4973617263497019 1106 40-41 0.4492906777768084 1106 42-43 0.43383615197009817 1106 44-45 0.3957404413581713 1106 46-47 0.4495018796916881 1106 48-49 0.41807065285160405 1106 50-51 0.4937754146990585 1106 52-53 0.4217778558927847 1106 54-55 0.38078797139289833 1106 56-57 0.423717178641688 1106 58-59 0.4111679855838517 1106 60-61 0.41871255501398963 1106 62-63 0.3265812263407355 1106 64-65 0.22232353487515866 1106 66-67 -10.52898622203778 1106 68-69 -8.39608138722777 1106 70-71 -6.8663537872081335 1106 72-73 -1.6118743759625787 1106 74-75 -0.4073144703167131 1106 76-77 -0.14867609933098436 1106 78-79 3.0517190006364636 1106 80-81 0.8706465440302686 1106 82-83 0.2111431890583546 1106 84-85 -5.654137736360802 1106 86-87 -6.094083863438609 1106 88-89 -1.4819339546698487 1106 90-91 -0.18820852706888402 1106 92-93 0.1672551034710743 1106 94-95 0.25356812484213975 1106 96-97 0.3274235579726472 1106 98-99 0.36504554860221106 1106 100 0.45276856279035016 1107 1 0.02882229843788764 1107 2 0.05592508142648356 1107 3 0.005302539114307336 1107 4 0.025406227394860537 1107 5 0.032135459180693715 1107 6 0.05539620627691022 1107 7 -12.367394683201525 1107 8 -6.824896049894015 1107 9 -2.61937637481099 1107 10-11 -0.5397592445871737 1107 12-13 0.06881396411172602 1107 14-15 0.18938265121791176 1107 16-17 0.18998435996342522 1107 18-19 0.28791716389289235 1107 20-21 0.2122323978879237 1107 22-23 0.2927187549174519 1107 24-25 0.3309288935234136 1107 26-27 0.23485248913036116 1107 28-29 0.2237438889790937 1107 30-31 0.20895424952456665 1107 32-33 0.2100705015925186 1107 34-35 0.22998868330780908 1107 36-37 0.312194161919102 1107 38-39 0.3235636217679456 1107 40-41 0.2933959699540978 1107 42-43 0.2581647878721256 1107 44-45 0.2209469270471942 1107 46-47 0.2839012312154523 1107 48-49 0.22751558593476773 1107 50-51 0.2813247418775404 1107 52-53 0.2629189384561954 1107 54-55 0.20845433721010664 1107 56-57 0.18973346251025447 1107 58-59 0.22645488450657325 1107 60-61 0.1563875516708535 1107 62-63 0.144842305140358 1107 64-65 0.013235611371051448 1107 66-67 -10.60860259298136 1107 68-69 -8.544901478885194 1107 70-71 -7.018509333910863 1107 72-73 -1.8085093034447297 1107 74-75 -0.5262138026082326 1107 76-77 -0.27731323295360255 1107 78-79 2.980325221860607 1107 80-81 0.8101569498683894 1107 82-83 0.12264528447182244 1107 84-85 -5.710761293326673 1107 86-87 -6.151926163192396 1107 88-89 -1.582719939711506 1107 90-91 -0.34457094147211365 1107 92-93 0.01494195744969673 1107 94-95 0.0900534653806595 1107 96-97 0.15936254189609755 1107 98-99 0.23143383702544895 1107 100 0.3251078983136928 1108 1 -0.03551646691450472 1108 2 -0.008390672739768235 1108 3 -0.010290437694830956 1108 4 -0.04959774612143519 1108 5 -0.07110780708020314 1108 6 0.0072252674231094716 1108 7 -12.382421884183959 1108 8 -6.880585152536121 1108 9 -2.6733568654463085 1108 10-11 -0.5742625144065485 1108 12-13 0.006161829871068392 1108 14-15 0.17555460155794833 1108 16-17 0.14532316647878218 1108 18-19 0.19015498328879943 1108 20-21 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0.06305770798499566 2214 92-93 0.04066289754966235 2214 94-95 0.12910067961245986 2214 96-97 2.554663509002353E-4 2214 98-99 0.07345690239680991 2214 100 0.07955725960329829 2215 1 -0.22565415945885192 2215 2 -0.3695769285570343 2215 3 -0.3003889622070339 2215 4 -0.2632719544183786 2215 5 -0.2024820954354567 2215 6 -0.42961628257111784 2215 7 3.5451853153121355 2215 8 1.6874075005648805 2215 9 0.27725804147795685 2215 10-11 -0.27045400022802113 2215 12-13 -0.5867866156430424 2215 14-15 -0.7036239077238591 2215 16-17 -0.7293736432138971 2215 18-19 -0.6753281402577187 2215 20-21 -0.7417670278690522 2215 22-23 -0.6733151953369898 2215 24-25 -0.6036017374580567 2215 26-27 -0.7048010071445105 2215 28-29 -0.5627383249943563 2215 30-31 -0.5983046124150135 2215 32-33 -0.605804904152528 2215 34-35 -0.5963087526635462 2215 36-37 -0.5658354862239037 2215 38-39 -0.6536321238004206 2215 40-41 -0.6960553309525537 2215 42-43 -0.6473243349470579 2215 44-45 -0.6518970364330698 2215 46-47 -0.6138870580725921 2215 48-49 -0.5661048785614113 2215 50-51 -0.5861691077761151 2215 52-53 -0.5533313454013964 2215 54-55 -0.6266188693976957 2215 56-57 -0.47531097227994934 2215 58-59 -0.5400709738274045 2215 60-61 -0.5283409230326228 2215 62-63 -0.599388193252139 2215 64-65 -0.470333006727472 2215 66-67 2.947291278719259 2215 68-69 2.2362723285377797 2215 70-71 1.7994939515118773 2215 72-73 0.07270266598820996 2215 74-75 -0.25780385056688004 2215 76-77 -0.3236639511517154 2215 78-79 2.791339056772575 2215 80-81 0.5710949451792153 2215 82-83 -0.17566524002257466 2215 84-85 1.5630484402358995 2215 86-87 1.745778730004485 2215 88-89 0.28217396194037647 2215 90-91 -0.13834611721865997 2215 92-93 -0.3796939950375666 2215 94-95 -0.367132830476816 2215 96-97 -0.40384694332505333 2215 98-99 -0.5053694469145 2215 100 -0.402204761286054 2216 1 -0.7663957412677256 2216 2 -0.5548007629921123 2216 3 -0.7116732808744288 2216 4 -0.713863494360325 2216 5 -0.5544014706441445 2216 6 -0.8370855980510257 2216 7 3.2566765787238943 2216 8 1.377206532232357 2216 9 0.06583028150920711 2216 10-11 -0.6355654906314712 2216 12-13 -0.997149670819816 2216 14-15 -1.210285427991181 2216 16-17 -1.375169062444762 2216 18-19 -1.8411341430225647 2216 20-21 -1.3563576857810276 2216 22-23 -1.1864472059976165 2216 24-25 -1.2091916071240476 2216 26-27 -1.6384424247148957 2216 28-29 -1.8138113481041174 2216 30-31 -1.738455654537269 2216 32-33 -2.6247064252970134 2216 34-35 -1.6545861554907688 2216 36-37 -1.3479673169925164 2216 38-39 -1.590376720677611 2216 40-41 -1.6244577127259632 2216 42-43 -1.2335580973377986 2216 44-45 -1.3549492289580982 2216 46-47 -1.5913375823270854 2216 48-49 -1.6579141739750582 2216 50-51 -2.142139541880141 2216 52-53 -1.87774063270804 2216 54-55 -1.521078498200744 2216 56-57 -1.5303929721896736 2216 58-59 -1.8636872920214174 2216 60-61 -1.99273176883586 2216 62-63 -1.89211749475254 2216 64-65 -1.5625236656638393 2216 66-67 1.9758261560129817 2216 68-69 1.1076038186723949 2216 70-71 0.7580040394768943 2216 72-73 -1.0972854326178094 2216 74-75 -1.862418815907695 2216 76-77 -1.5114211534780964 2216 78-79 1.0936834673720774 2216 80-81 -0.39874783091772414 2216 82-83 -1.3536133715326883 2216 84-85 0.5039871804506042 2216 86-87 0.5090101766799506 2216 88-89 -0.7701625295338275 2216 90-91 -1.1047295492931823 2216 92-93 -1.1574490720225548 2216 94-95 -1.17809263538987 2216 96-97 -1.1697032065582782 2216 98-99 -1.1956478909169022 2216 100 -2.415252806827386 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 111352.0 3 21494.0 4 13278.0 5 11632.0 6 10899.0 7 12060.0 8 13506.0 9 15956.0 10 18122.0 11 20783.0 12 23265.0 13 26877.0 14 30950.0 15 34115.0 16 36352.0 17 38199.0 18 36318.0 19 33708.0 20 32169.0 21 32921.0 22 35179.0 23 40315.0 24 48635.0 25 61557.0 26 76693.0 27 94918.0 28 117937.0 29 151934.0 30 188361.0 31 232220.0 32 313509.0 33 444935.0 34 691226.0 35 988607.0 36 1052822.0 37 1858729.0 38 2288253.0 39 825785.0 40 2126.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.25061361308372 20.942194400358606 15.0185216711137 22.788670315443973 2 14.970165246792607 20.31186043337047 41.38140890063116 23.33656541920577 3 18.21616395468499 25.302296828387632 30.699391991231938 25.782147225695436 4 12.51510914919765 17.267488620244343 39.32283650812581 30.894565722432205 5 13.032790005495931 37.96904752274705 35.87245515820428 13.125707313552743 6 30.319893132684495 38.31758329182063 18.078063699066398 13.28445987642847 7 26.746533126334153 32.838540866602365 22.76883674192027 17.646089265143207 8 24.211531814062937 37.22070051100881 20.741957551642383 17.82581012328587 9 24.28016027840646 17.758661863059526 22.232576969748397 35.728600888785614 10-11 23.364326862712073 27.625621586373978 29.279904025666113 19.730147525247833 12-13 23.72829497158767 24.980994175380168 29.436133936219534 21.85457691681263 14-15 21.18467178385711 27.444832056315725 26.515383045307566 24.855113114519597 16-17 20.73391358688869 30.769175484619378 27.335767396578337 21.1611435319136 18-19 20.69215104299822 29.800716655149333 29.13904432300058 20.36808797885186 20-21 21.73030177254531 28.926493331431345 28.74032596339878 20.602878932624563 22-23 21.515353951982693 28.732760186270482 28.743813255276528 21.0080726064703 24-25 21.329913061425216 28.874529042654633 28.704217622714083 21.091340273206065 26-27 21.072330184259975 28.92636872326348 28.72717022596829 21.27413086650826 28-29 21.050543052591685 28.941491799367093 28.91236721324996 21.09559793479126 30-31 21.397416080201456 29.22064867729473 28.487602274334765 20.89433296816905 32-33 21.047168956552408 29.02684201166292 28.828808645464466 21.097180386320204 34-35 21.253129184865 28.821778938312047 28.763296774256865 21.16179510256609 36-37 21.432297680336937 29.016474380392303 28.469783504028946 21.08144443524181 38-39 21.2707843648264 29.09599927277709 28.63005022655829 21.00316613583822 40-41 21.234585120597156 28.889350507323453 28.722414477874107 21.15364989420528 42-43 21.17904384660474 28.856435035529937 28.69658676103382 21.267934356831507 44-45 21.27617791867136 28.960534172561935 28.583638660498284 21.17964924826842 46-47 21.29351436636786 29.017119850652108 28.513495991311306 21.175869791668735 48-49 21.196430652304432 28.949929534869668 28.540015931419262 21.313623881406638 50-51 21.218170008969377 28.910759843515677 28.500684400816205 21.370385746698737 52-53 21.20131098645069 28.888571009106066 28.557954556606774 21.35216344783647 54-55 21.293314195754277 29.08924738420428 28.42155021726056 21.195888202780882 56-57 21.199997779435932 28.911466606235603 28.439175429220658 21.449360185107807 58-59 21.11160225003529 28.689605442845835 28.679999651058957 21.518792656059915 60-61 21.287345968820233 28.953926258539227 28.3967751196324 21.36195265300814 62-63 21.11240903742008 29.02809959515571 28.440047609613462 21.419443757810754 64-65 21.221864664891942 28.770239488511717 28.497478384810154 21.510417461786187 66-67 21.089755458334693 28.813657684180992 28.41284655977423 21.68374029771008 68-69 21.538668960709416 28.94004436940917 28.279792418556355 21.24149425132506 70-71 21.282962447761502 28.773152177749083 28.377351005037184 21.56653436945223 72-73 21.167323730541987 28.88011096447275 28.514705201548878 21.437860103436385 74-75 21.244016572651972 28.9105707385836 28.36581372802735 21.47959896073708 76-77 21.218861302574272 28.881405503888285 28.24997940436124 21.649753789176202 78-79 21.37837672985386 28.877447650324857 28.334494202397014 21.40968141742427 80-81 21.209084655881927 28.7942239977865 28.43698853728709 21.559702809044488 82-83 21.187169661307887 28.80876577558556 28.530546430941833 21.47351813216472 84-85 21.300645428504577 28.647700192353696 28.382975116505815 21.66867926263591 86-87 21.334146369753064 28.79137794109002 28.313717392254752 21.560758296902172 88-89 21.197220838772406 28.66928726868411 28.39567647259421 21.737815419949275 90-91 21.163772646309265 28.579813919897973 28.432331770893615 21.82408166289915 92-93 21.286607078154084 28.704356799065277 28.304997843325825 21.70403827945481 94-95 21.235909164359292 28.490596526499345 28.416919213698904 21.856575095442455 96-97 21.184862265951253 28.54646030310165 28.40001982676365 21.868657604183447 98-99 21.25162652074826 28.549513023988016 28.3058007215851 21.89305973367862 100 21.27262561192503 28.508419731496517 28.265791085969887 21.953163570608563 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1930.0 1 1570.5 2 1381.5 3 1617.5 4 1558.5 5 1402.0 6 1343.5 7 1343.5 8 1415.0 9 1469.5 10 1404.0 11 1329.0 12 1430.5 13 1514.0 14 1659.0 15 2000.0 16 2467.0 17 3203.5 18 4394.5 19 6276.5 20 9433.0 21 13846.5 22 20364.5 23 30675.5 24 43878.0 25 60122.0 26 81973.0 27 109265.0 28 141913.5 29 175439.5 30 206577.0 31 237136.5 32 265692.0 33 291027.0 34 314484.0 35 336144.0 36 352248.0 37 365343.0 38 376953.5 39 382671.0 40 384988.5 41 383569.0 42 375234.5 43 366286.0 44 357401.5 45 347356.5 46 337469.5 47 325046.0 48 313269.5 49 304377.5 50 293092.5 51 276823.0 52 258993.0 53 243549.0 54 230497.0 55 215718.5 56 195387.5 57 172637.0 58 152485.5 59 134672.0 60 115124.0 61 94949.5 62 78352.0 63 63823.5 64 50717.5 65 39804.0 66 31124.0 67 23860.0 68 17952.0 69 13603.5 70 9962.5 71 7223.0 72 5344.0 73 3823.5 74 2661.5 75 1760.5 76 1089.0 77 675.0 78 481.5 79 344.0 80 235.0 81 168.5 82 127.0 83 105.0 84 80.0 85 55.0 86 54.0 87 54.0 88 46.5 89 35.0 90 24.0 91 25.0 92 17.5 93 16.0 94 15.5 95 16.0 96 17.5 97 14.5 98 15.0 99 26.0 100 54.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.019270999119025876 2 0.002973919617133623 3 0.001764525639499283 4 0.002636875393858479 5 0.0023097442359737807 6 0.004183313594767963 7 11.58979100978152 8 3.965226156178164E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.766964848369256E-4 18-19 0.0 20-21 0.0 22-23 9.91306539044541E-6 24-25 0.0 26-27 2.032178405041309E-4 28-29 0.0 30-31 0.0 32-33 2.9739196171336233E-5 34-35 5.9478392342672465E-5 36-37 3.469572886655894E-5 38-39 5.9478392342672465E-5 40-41 4.956532695222705E-6 42-43 5.9478392342672465E-5 44-45 2.032178405041309E-4 46-47 0.00108052412755855 48-49 8.277409601021919E-4 50-51 4.064356810082618E-4 52-53 2.577397001515807E-4 54-55 3.9652261561781644E-4 56-57 0.00168522111637572 58-59 3.2713115788469857E-4 60-61 0.001278785435367458 62-63 0.002364266095621231 64-65 0.0011400025199012223 66-67 5.795103679263959 68-69 5.44592586395091 70-71 0.003063137205647632 72-73 0.0014274814162241391 74-75 0.003524094746303343 76-77 0.0060469698881717 78-79 5.412484137856242 80-81 0.005080446012603273 82-83 0.00507548947990805 84-85 5.795480375748796 86-87 0.004044530679301728 88-89 0.0019132216203559642 90-91 0.0037124429887218065 92-93 0.004024704548520837 94-95 0.0046294015373380066 96-97 0.003192007055723422 98-99 0.0059329696361815785 100 0.006711145269331542 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0087697E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.37561958851369 #Duplication Level Percentage of deduplicated Percentage of total 1 89.88842563859967 76.74280032731484 2 7.280990129814183 12.43238087101477 3 1.5374549468855299 3.937835060393325 4 0.5432431587189964 1.8551888505142236 5 0.27129754551556096 1.1581098020617007 6 0.1514314176373055 0.7757130663571161 7 0.09093111385566723 0.5434310129710889 8 0.059110844609556554 0.4037299986352996 9 0.04187175784461358 0.32173445423196834 >10 0.13333393204282717 1.7009983880323605 >50 0.0015981295077566403 0.0917074682673571 >100 3.113849683704147E-4 0.036370700205943886 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 9.913065390445411E-6 0.0 3 0.0 0.0 0.0 9.913065390445411E-6 0.0 4 0.0 0.0 0.0 9.913065390445411E-6 0.0 5 0.0 0.0 0.0 9.913065390445411E-6 0.0 6 0.0 0.0 0.0 9.913065390445411E-6 0.0 7 0.0 0.0 0.0 3.9652261561781646E-5 0.0 8 0.0 0.0 0.0 3.9652261561781646E-5 0.0 9 4.9565326952227056E-5 0.0 0.0 6.939145773311788E-5 0.0 10-11 5.452185964744976E-5 0.0 0.0 7.434799042834058E-5 0.0 12-13 5.9478392342672465E-5 0.0 0.0 1.0408718659967681E-4 0.0 14-15 6.939145773311788E-5 4.956532695222706E-6 0.0 1.5860904624712658E-4 0.0 16-17 6.939145773311788E-5 9.913065390445411E-6 0.0 2.0817437319935363E-4 0.0 18-19 6.939145773311788E-5 9.913065390445411E-6 0.0 2.2304397128502173E-4 0.0 20-21 6.939145773311788E-5 9.913065390445411E-6 4.956532695222706E-6 2.478266347611353E-4 0.0 22-23 6.939145773311788E-5 9.913065390445411E-6 9.913065390445411E-6 3.5687035405603483E-4 0.0 24-25 6.939145773311788E-5 1.4869598085668118E-5 9.913065390445411E-6 5.105228676079386E-4 0.0 26-27 6.939145773311788E-5 1.9826130780890823E-5 9.913065390445411E-6 9.962630717397637E-4 0.0 28-29 6.939145773311788E-5 2.4782663476113528E-5 9.913065390445411E-6 0.0025724404688205844 0.0 30-31 6.939145773311788E-5 2.9739196171336233E-5 9.913065390445411E-6 0.007132450548425473 0.0 32-33 6.939145773311788E-5 2.9739196171336233E-5 9.913065390445411E-6 0.018016996347134533 0.0 34-35 6.939145773311788E-5 3.469572886655894E-5 9.913065390445411E-6 0.035503643695880244 0.0 36-37 6.939145773311788E-5 5.947839234267247E-5 9.913065390445411E-6 0.06494049137280788 0.0 38-39 6.939145773311788E-5 8.426105581878599E-5 9.913065390445411E-6 0.10543536349277738 0.0 40-41 6.939145773311788E-5 8.92175885140087E-5 9.913065390445411E-6 0.14792276175622643 0.0 42-43 6.939145773311788E-5 1.2886985007579035E-4 9.913065390445411E-6 0.19219946832265083 0.0 44-45 6.939145773311788E-5 1.2886985007579035E-4 9.913065390445411E-6 0.2499331611566049 0.0 46-47 6.939145773311788E-5 1.2886985007579035E-4 9.913065390445411E-6 0.32166410232186793 0.0 48-49 6.939145773311788E-5 1.2886985007579035E-4 9.913065390445411E-6 0.39831192392079184 0.0 50-51 6.939145773311788E-5 1.2886985007579035E-4 9.913065390445411E-6 0.4784689706679334 0.0 52-53 6.939145773311788E-5 1.2886985007579035E-4 9.913065390445411E-6 0.5609655008472201 0.0 54-55 6.939145773311788E-5 1.4869598085668115E-4 9.913065390445411E-6 0.6515709185158911 0.0 56-57 6.939145773311788E-5 1.4869598085668115E-4 9.913065390445411E-6 0.7532095779641279 0.0 58-59 6.939145773311788E-5 1.4869598085668115E-4 9.913065390445411E-6 0.8679334837277528 0.0 60-61 6.939145773311788E-5 1.4869598085668115E-4 9.913065390445411E-6 0.9907315812518953 0.0 62-63 7.930452312356329E-5 1.4869598085668115E-4 9.913065390445411E-6 1.114912551398005 0.0 64-65 7.930452312356329E-5 1.4869598085668115E-4 9.913065390445411E-6 1.2510833741338583 0.0 66-67 7.930452312356329E-5 1.6356557894234928E-4 9.913065390445411E-6 1.406554935184909 0.0 68-69 7.930452312356329E-5 1.6852211163757199E-4 9.913065390445411E-6 1.5673795515468 0.0 70-71 7.930452312356329E-5 1.6852211163757199E-4 9.913065390445411E-6 1.7652344236747 0.0 72-73 7.930452312356329E-5 1.6852211163757199E-4 9.913065390445411E-6 1.9762290639776352 0.0 74-75 7.930452312356329E-5 1.6852211163757199E-4 9.913065390445411E-6 2.1752090690273507 0.0 76-77 7.930452312356329E-5 1.6852211163757199E-4 9.913065390445411E-6 2.387760060596586 0.0 78-79 7.930452312356329E-5 1.6852211163757199E-4 9.913065390445411E-6 2.6399484441295176 0.0 80-81 7.930452312356329E-5 1.6852211163757199E-4 9.913065390445411E-6 2.947699559175895 0.0 82-83 7.930452312356329E-5 1.734786443327947E-4 9.913065390445411E-6 3.265666088107127 0.0 84-85 7.930452312356329E-5 1.784351770280174E-4 9.913065390445411E-6 3.5899571527574627 9.913065390445411E-6 86-87 8.92175885140087E-5 1.784351770280174E-4 9.913065390445411E-6 3.9771961826371274 9.913065390445411E-6 88 8.92175885140087E-5 1.784351770280174E-4 9.913065390445411E-6 4.302121683472452 9.913065390445411E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9455 0.0 43.63013 1 GTATCAA 14865 0.0 35.635956 1 TCAACGC 14965 0.0 33.88767 4 ATCAACG 15205 0.0 33.35278 3 CAACGCA 16495 0.0 30.837135 5 TATCAAC 17945 0.0 28.745132 2 AACGCAG 18140 0.0 28.04024 6 ACGCAGA 20630 0.0 24.307346 7 CGCAGAG 21375 0.0 23.238535 8 GTACATG 24820 0.0 22.518208 1 TACATGG 25080 0.0 21.179565 2 ACATGGG 25825 0.0 19.934366 3 GTGGTAT 5790 0.0 18.74003 1 GCAGAGT 24565 0.0 18.710674 9 GAGTACA 19530 0.0 17.034292 1 AGAGTAC 21175 0.0 16.36515 10-11 CATGGGG 18200 0.0 16.27991 4 CAGAGTA 23860 0.0 16.144659 10-11 GTATAGG 3205 0.0 15.64988 1 CCTATAC 2830 0.0 14.465996 3 >>END_MODULE