FastQCFastQC Report
Wed 25 May 2016
SRR2049524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049524_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10087697
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258400.25615360968910944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258290.2560445659698145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150560.14925111251854611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178950.056.1386831
GTATCAA318950.039.8954121
ATCAACG394200.031.8323553
TCAACGC396550.031.6674464
GTGGTAT74650.030.9005221
TATCAAC409600.030.8078372
CAACGCA412900.030.42665
AACGCAG433750.029.028176
TGGTATC84000.026.3257642
ACGCAGA484650.025.6489037
CGCAGAG484450.025.4363658
GCAGAGT526700.022.967659
GAGTACT360100.021.61920412-13
GTACATG264950.021.3392221
CAGAGTA513700.020.5984110-11
TACATGG267450.020.3365652
AGAGTAC488850.020.16016810-11
GTACTTT400400.019.4197714-15
AGTACTT384000.019.08646612-13
ACATGGG272300.019.0067313
Produced by FastQC (version 0.11.5)