FastQCFastQC Report
Wed 25 May 2016
SRR2049523_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049523_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7725678
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73300.045.2229231
GTATCAA118200.035.543051
TCAACGC122500.033.0336844
ATCAACG123200.032.887573
CAACGCA131850.030.7686065
TATCAAC140900.029.0106772
AACGCAG147800.027.3440076
GTACATG223700.025.1474911
TACATGG225650.023.9260642
ACGCAGA173050.023.0871247
CGCAGAG174200.022.7919148
ACATGGG231300.022.4557863
GTGGTAT44750.021.6384961
CATGGGG149500.019.2215164
TGGTATC45950.019.0623552
GCAGAGT198850.018.6021069
GAGTACA169700.017.2994021
GCCGTAT9650.016.87186294
CAGAGTA190550.016.57657210-11
GTATAGG26300.016.1689071
Produced by FastQC (version 0.11.5)