FastQCFastQC Report
Wed 25 May 2016
SRR2049523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049523_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7725678
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195380.2528968978515542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190500.24658030013676469No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105200.13616927860570943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142500.056.444461
GTATCAA254850.039.8393671
GTGGTAT58050.033.0983431
ATCAACG316800.031.8803143
TCAACGC320050.031.5712834
TATCAAC325250.031.0231232
CAACGCA333100.030.4201585
TGGTATC63950.029.0693022
AACGCAG353450.028.7348236
CGCAGAG397600.025.1886438
ACGCAGA404200.024.9982647
GCAGAGT429200.022.9399269
GTACATG235550.022.4314881
TACATGG238950.021.4682942
GAGTACT290750.021.32027212-13
CAGAGTA421500.020.43215210-11
ACATGGG242700.020.1476233
AGAGTAC400050.020.0239810-11
GTACTTT314400.019.58944514-15
AGTACTT300400.019.0630912-13
Produced by FastQC (version 0.11.5)