##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049523_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7725678
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.700833117818267	31.0	31.0	34.0	30.0	34.0
2	31.747528178109416	31.0	31.0	34.0	30.0	34.0
3	31.80675353024032	33.0	31.0	34.0	30.0	34.0
4	35.529219441970014	37.0	35.0	37.0	33.0	37.0
5	35.689224427940175	37.0	35.0	37.0	33.0	37.0
6	35.787108393593414	37.0	35.0	37.0	35.0	37.0
7	35.76086500110411	37.0	35.0	37.0	33.0	37.0
8	35.754174714504025	37.0	35.0	37.0	33.0	37.0
9	37.48103066164549	39.0	37.0	39.0	34.0	39.0
10-11	37.45113373868287	39.0	37.0	39.0	34.0	39.0
12-13	37.44909553569279	39.0	37.0	39.0	34.0	39.0
14-15	38.71385844711622	40.0	38.0	41.0	34.0	41.0
16-17	38.72476292695606	40.0	38.0	41.0	34.5	41.0
18-19	38.79505054443118	40.0	38.0	41.0	35.0	41.0
20-21	38.825600355075636	40.0	38.0	41.0	35.0	41.0
22-23	38.80759500978425	40.0	38.0	41.0	35.0	41.0
24-25	38.77908333741065	40.0	38.0	41.0	35.0	41.0
26-27	38.69284883475599	40.0	38.0	41.0	34.5	41.0
28-29	38.60916899461769	40.0	38.0	41.0	34.0	41.0
30-31	38.499183178486085	40.0	38.0	41.0	34.0	41.0
32-33	38.365696706489715	40.0	38.0	41.0	34.0	41.0
34-35	38.269790107742	40.0	38.0	41.0	34.0	41.0
36-37	38.137272741628635	40.0	38.0	41.0	34.0	41.0
38-39	38.05415175211807	40.0	38.0	41.0	33.0	41.0
40-41	37.93708293304484	40.0	37.5	41.0	33.0	41.0
42-43	37.800462043590215	40.0	37.0	41.0	33.0	41.0
44-45	37.6276895697698	40.0	37.0	41.0	33.0	41.0
46-47	37.38608863843406	40.0	37.0	41.0	32.5	41.0
48-49	37.15873590641495	40.0	37.0	41.0	31.5	41.0
50-51	36.95305382906199	40.0	37.0	41.0	31.0	41.0
52-53	36.85550459131225	40.0	36.0	41.0	31.0	41.0
54-55	36.73647361435462	40.0	36.0	41.0	31.0	41.0
56-57	36.57223501937305	39.0	36.0	41.0	31.0	41.0
58-59	36.40656871798178	39.0	35.0	41.0	31.0	41.0
60-61	36.222855909863185	39.0	35.0	41.0	30.0	41.0
62-63	36.03013638414648	39.0	35.0	41.0	30.0	41.0
64-65	35.76371549526139	38.5	35.0	40.0	30.0	41.0
66-67	35.51083503609651	38.0	35.0	40.0	29.0	41.0
68-69	35.204703004707156	37.0	35.0	40.0	29.0	41.0
70-71	34.911956529899385	37.0	34.0	39.5	29.0	41.0
72-73	34.59550255653937	36.0	34.0	39.0	29.0	41.0
74-75	34.25018017836105	36.0	34.0	39.0	28.0	41.0
76-77	33.95086107652946	35.5	34.0	38.5	28.0	40.0
78-79	33.61282990308423	35.0	34.0	37.0	28.0	39.0
80-81	33.29499520948194	35.0	34.0	37.0	28.0	39.0
82-83	32.99613406098467	35.0	34.0	36.5	27.5	39.0
84-85	32.71511872485496	35.0	33.0	36.0	27.0	37.0
86-87	32.44127855186302	35.0	33.0	36.0	26.5	37.0
88-89	32.21847745919517	35.0	33.0	35.0	26.0	37.0
90-91	32.05967540971808	35.0	33.0	35.0	26.0	36.0
92-93	31.8977919478394	35.0	33.0	35.0	26.0	36.0
94-95	31.715131021510345	35.0	33.0	35.0	25.5	36.0
96-97	31.54556137338367	35.0	33.0	35.0	25.0	36.0
98-99	31.36183698052132	35.0	33.0	35.0	25.0	35.0
100	31.246280002868357	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3795924433243627
1101	2	0.41666534469015915
1101	3	0.5159517618871625
1101	4	0.39489996285280426
1101	5	0.3121559231504776
1101	6	0.2965655896126478
1101	7	0.29736485248267286
1101	8	0.32700620778411604
1101	9	0.35927794173191785
1101	10-11	0.38541192169900995
1101	12-13	0.3298331724662589
1101	14-15	0.5058310315059771
1101	16-17	0.531496091473727
1101	18-19	0.48653961673315393
1101	20-21	0.4579919302573927
1101	22-23	0.4914046108550707
1101	24-25	0.5311094022198901
1101	26-27	0.5178183255991797
1101	28-29	0.5075458418867527
1101	30-31	0.4926521217746185
1101	32-33	0.5677414814005886
1101	34-35	0.5782562880848587
1101	36-37	0.5733393823430077
1101	38-39	0.5513839112816754
1101	40-41	0.6224824387760179
1101	42-43	0.5407933850801001
1101	44-45	0.571005638002049
1101	46-47	0.6169799902127266
1101	48-49	0.6749678680514464
1101	50-51	0.673854441715477
1101	52-53	0.628901281608627
1101	54-55	0.6203408795475838
1101	56-57	0.5987065181670488
1101	58-59	0.5854115811132417
1101	60-61	0.6725242473396236
1101	62-63	0.6286882746008828
1101	64-65	0.6675323603732579
1101	66-67	0.6453253953950977
1101	68-69	0.6200940998864155
1101	70-71	0.6075418453095836
1101	72-73	0.6260250035625248
1101	74-75	0.6370733731747791
1101	76-77	0.5704649480742603
1101	78-79	0.5665087880079653
1101	80-81	0.5557061593886772
1101	82-83	0.6136492496219503
1101	84-85	0.61436764938464
1101	86-87	0.6729318506928053
1101	88-89	0.662258365331752
1101	90-91	0.6611478114906078
1101	92-93	0.6950300237513716
1101	94-95	0.6523054021197083
1101	96-97	0.6903815268846998
1101	98-99	0.7522148440961693
1101	100	0.7722060704251454
1102	1	0.263849830863613
1102	2	0.31742189384939223
1102	3	0.40262494513648406
1102	4	0.36772870045043504
1102	5	0.2992519244684786
1102	6	0.23691299970160173
1102	7	0.2714285242819372
1102	8	0.2822281673253286
1102	9	0.38030626759194774
1102	10-11	0.3259459775549658
1102	12-13	0.313917172812225
1102	14-15	0.38528687785949955
1102	16-17	0.4266502030815502
1102	18-19	0.44844586025503475
1102	20-21	0.42371262871449744
1102	22-23	0.4027558368046016
1102	24-25	0.3821801491841228
1102	26-27	0.33716753604288385
1102	28-29	0.3819084837595028
1102	30-31	0.452320059719419
1102	32-33	0.4653704507889813
1102	34-35	0.5054559671437957
1102	36-37	0.5641646772702913
1102	38-39	0.5094954167730918
1102	40-41	0.5270925600951273
1102	42-43	0.5877708544957869
1102	44-45	0.5671436826752156
1102	46-47	0.5387966756781992
1102	48-49	0.5712128804153522
1102	50-51	0.6568172757932302
1102	52-53	0.5869976796707661
1102	54-55	0.5867366128955069
1102	56-57	0.5938353536400527
1102	58-59	0.5662215115443558
1102	60-61	0.5234822394419609
1102	62-63	0.5151664289162454
1102	64-65	0.5317445319972052
1102	66-67	0.5999590009787426
1102	68-69	0.5801831716684873
1102	70-71	0.5746820089484999
1102	72-73	0.5703293570981529
1102	74-75	0.5443917892830186
1102	76-77	0.542333407826888
1102	78-79	0.45846605107870886
1102	80-81	0.5355272686842838
1102	82-83	0.5386177905849294
1102	84-85	0.5110989867391069
1102	86-87	0.5111709322826741
1102	88-89	0.5547955991931062
1102	90-91	0.5837948483766695
1102	92-93	0.5856810070575946
1102	94-95	0.5810792297205154
1102	96-97	0.5531714727069001
1102	98-99	0.6474707187397151
1102	100	0.6901752115063751
1103	1	0.3071401347339062
1103	2	0.36865174064966766
1103	3	0.44571679415567544
1103	4	0.3682217182793366
1103	5	0.28682716701504063
1103	6	0.308690258158272
1103	7	0.2881378494680007
1103	8	0.3250241044807325
1103	9	0.4003159795710687
1103	10-11	0.37135377951152293
1103	12-13	0.33126882064507157
1103	14-15	0.4457383837220945
1103	16-17	0.4512042276515018
1103	18-19	0.44766477096215596
1103	20-21	0.4455987254093827
1103	22-23	0.4450515901742875
1103	24-25	0.4640884687756781
1103	26-27	0.43514875954991794
1103	28-29	0.44408732243611837
1103	30-31	0.5149446209099509
1103	32-33	0.46454805186269255
1103	34-35	0.5091562986325329
1103	36-37	0.5904674482193357
1103	38-39	0.5702259734246127
1103	40-41	0.5918648850013
1103	42-43	0.5753671733859278
1103	44-45	0.6380972776249791
1103	46-47	0.6300028663904413
1103	48-49	0.6562100646158484
1103	50-51	0.7249119068897656
1103	52-53	0.7106539363098179
1103	54-55	0.6826491511279968
1103	56-57	0.724808810936004
1103	58-59	0.6925251255471352
1103	60-61	0.7084559867484046
1103	62-63	0.6833213383554124
1103	64-65	0.72314513090258
1103	66-67	0.7401126390928638
1103	68-69	0.7568362314477426
1103	70-71	0.7194447061809228
1103	72-73	0.7185828869641782
1103	74-75	0.6957854414255564
1103	76-77	0.7032147867930405
1103	78-79	0.672613251634445
1103	80-81	0.6509394713813776
1103	82-83	0.6708488311492999
1103	84-85	0.7090018905534166
1103	86-87	0.7468920467322775
1103	88-89	0.7443968684948175
1103	90-91	0.6676286184118396
1103	92-93	0.7178971255707154
1103	94-95	0.7358869123006642
1103	96-97	0.7975712353029678
1103	98-99	0.7945791287066335
1103	100	0.8428546049123433
1104	1	0.2931627747437844
1104	2	0.3103321200493454
1104	3	0.3882174443118238
1104	4	0.3544864311788203
1104	5	0.2663958085454041
1104	6	0.28513650079271713
1104	7	0.2265208439913451
1104	8	0.2872445085671629
1104	9	0.3025723091001282
1104	10-11	0.3311462243518122
1104	12-13	0.32620276171139295
1104	14-15	0.4413533393848965
1104	16-17	0.40331040754875147
1104	18-19	0.3762912230734514
1104	20-21	0.40249147380931305
1104	22-23	0.383851509317104
1104	24-25	0.38622739813151696
1104	26-27	0.3873772416827066
1104	28-29	0.3528240594423835
1104	30-31	0.4460663876776536
1104	32-33	0.44042344205228545
1104	34-35	0.40934279180147826
1104	36-37	0.4536629714627054
1104	38-39	0.4619411812482781
1104	40-41	0.504662834880591
1104	42-43	0.444019213648815
1104	44-45	0.4853376236691531
1104	46-47	0.5347013473648445
1104	48-49	0.5344266882072759
1104	50-51	0.5921858078957243
1104	52-53	0.5633100255687751
1104	54-55	0.5326695955663041
1104	56-57	0.4965461585904265
1104	58-59	0.5251078833292553
1104	60-61	0.48247229473600584
1104	62-63	0.5245396082419447
1104	64-65	0.5360122965209371
1104	66-67	0.4884201234450032
1104	68-69	0.49675737268467657
1104	70-71	0.5201798812781391
1104	72-73	0.49931037909517784
1104	74-75	0.5000509068951686
1104	76-77	0.4817510225461774
1104	78-79	0.4681525858898965
1104	80-81	0.4804566757789672
1104	82-83	0.4693537213208572
1104	84-85	0.519757870647517
1104	86-87	0.5252610063213297
1104	88-89	0.4796431184938612
1104	90-91	0.47797331240884944
1104	92-93	0.5225523203375531
1104	94-95	0.5214295682978616
1104	96-97	0.5132511989095647
1104	98-99	0.6123282190633823
1104	100	0.6419057221452782
1105	1	0.26612734130041105
1105	2	0.3037692993655057
1105	3	0.3704808151403505
1105	4	0.3265216082686351
1105	5	0.244315322668605
1105	6	0.23370538631155569
1105	7	0.24545501103338552
1105	8	0.24184993620784212
1105	9	0.27725928407983247
1105	10-11	0.2720581073215982
1105	12-13	0.26749454554477836
1105	14-15	0.37035482955284493
1105	16-17	0.36074924050202384
1105	18-19	0.3546333722539714
1105	20-21	0.317772088441572
1105	22-23	0.3383686341219203
1105	24-25	0.33777960142862895
1105	26-27	0.36669005122594456
1105	28-29	0.4006583473410359
1105	30-31	0.3790063383951079
1105	32-33	0.3904334953137436
1105	34-35	0.3043473916062993
1105	36-37	0.4298191711699104
1105	38-39	0.3848407932310778
1105	40-41	0.39836014210667514
1105	42-43	0.4144874494155033
1105	44-45	0.399441890605047
1105	46-47	0.44349771340293387
1105	48-49	0.40720992009696033
1105	50-51	0.43710935316483557
1105	52-53	0.395307442940279
1105	54-55	0.397386879240301
1105	56-57	0.400474729900516
1105	58-59	0.41504775874400934
1105	60-61	0.40836788552481096
1105	62-63	0.37540191637715026
1105	64-65	0.3855136123647753
1105	66-67	0.37957910286517915
1105	68-69	0.39970351827528106
1105	70-71	0.36746761085510116
1105	72-73	0.3974702544443218
1105	74-75	0.4174465545019501
1105	76-77	0.36963142607285704
1105	78-79	0.299956512533484
1105	80-81	0.2769582734827196
1105	82-83	0.2976232824520366
1105	84-85	0.3260305381719846
1105	86-87	0.3806701567391002
1105	88-89	0.3289409622231645
1105	90-91	0.2974525265582031
1105	92-93	0.3486833546395758
1105	94-95	0.36127224867023955
1105	96-97	0.38209971142678967
1105	98-99	0.4295777432980792
1105	100	0.4993717864443532
1106	1	0.1452449061720067
1106	2	0.14819318685777816
1106	3	0.2924921409310208
1106	4	0.23104644777073702
1106	5	0.155805669326881
1106	6	0.19578687858822974
1106	7	0.18584508050856385
1106	8	0.21173224118886935
1106	9	0.15075451334293888
1106	10-11	0.21793965109739588
1106	12-13	0.1436514413610226
1106	14-15	0.24958866257237133
1106	16-17	0.25782155926883377
1106	18-19	0.23996302159373784
1106	20-21	0.214403244213905
1106	22-23	0.18157025169294627
1106	24-25	0.20292492427880404
1106	26-27	0.23368300808417075
1106	28-29	0.24015481850361198
1106	30-31	0.26169732599598916
1106	32-33	0.27790903189034566
1106	34-35	0.26605843886800074
1106	36-37	0.2865790359975904
1106	38-39	0.2596463461783216
1106	40-41	0.27697976178284023
1106	42-43	0.24580189382064077
1106	44-45	0.2757599402876423
1106	46-47	0.3029138726347824
1106	48-49	0.2858110942168821
1106	50-51	0.3152808472587054
1106	52-53	0.2988981328873521
1106	54-55	0.23059590740808744
1106	56-57	0.2990318434923793
1106	58-59	0.24666617135484614
1106	60-61	0.32426465255707626
1106	62-63	0.3423470307144356
1106	64-65	0.3353764098005243
1106	66-67	0.4179118668920907
1106	68-69	0.3131008293790032
1106	70-71	0.34753470093758665
1106	72-73	0.3602006952486505
1106	74-75	0.3801388424456249
1106	76-77	0.33719638744130975
1106	78-79	0.3234450357995655
1106	80-81	0.2685964140383277
1106	82-83	0.3172356942028358
1106	84-85	0.2710734859583468
1106	86-87	0.3284545616281278
1106	88-89	0.27655045064840067
1106	90-91	0.24332455244110918
1106	92-93	0.303935544942469
1106	94-95	0.28623845431886963
1106	96-97	0.32070985010825126
1106	98-99	0.39090998337082183
1106	100	0.4687638474882547
1107	1	0.15386551556542472
1107	2	0.20671307317823917
1107	3	0.24782038669833284
1107	4	0.2242982688710029
1107	5	0.18970595588069727
1107	6	0.16285832700285852
1107	7	0.1655053590335669
1107	8	0.18440244935353434
1107	9	0.18291428219399108
1107	10-11	0.15862155170953685
1107	12-13	0.15285510542722847
1107	14-15	0.2054571593005292
1107	16-17	0.17892427188434112
1107	18-19	0.20557394095243353
1107	20-21	0.2381847503815422
1107	22-23	0.2441643462662455
1107	24-25	0.233927911262505
1107	26-27	0.25478268798565296
1107	28-29	0.2674766451859938
1107	30-31	0.23843292837445773
1107	32-33	0.2786219558661571
1107	34-35	0.23075157544597147
1107	36-37	0.30146880950793076
1107	38-39	0.2899302821454768
1107	40-41	0.2475547239697562
1107	42-43	0.2410341867071537
1107	44-45	0.32807356905798457
1107	46-47	0.3058828551585435
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2216	96-97	-1.904507690116894
2216	98-99	-1.9095727107703588
2216	100	-1.9010278399969103
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	434.0
3	42.0
4	56.0
5	144.0
6	347.0
7	999.0
8	2084.0
9	3716.0
10	5653.0
11	8548.0
12	12179.0
13	17379.0
14	20509.0
15	27162.0
16	35451.0
17	50579.0
18	50370.0
19	26738.0
20	19704.0
21	19122.0
22	20468.0
23	21987.0
24	25991.0
25	32057.0
26	41564.0
27	53814.0
28	70857.0
29	93526.0
30	123106.0
31	158700.0
32	204710.0
33	267639.0
34	361464.0
35	518245.0
36	845743.0
37	1542338.0
38	2170792.0
39	868479.0
40	2982.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.775073299122326	21.336319608263175	15.005642976870401	22.8829641157441
2	15.73228084113946	21.004553266172753	39.62435327557001	23.63881261711778
3	17.898949179469966	25.68511471551296	30.989879592429247	25.42605651258783
4	12.755969185531532	17.77198355991475	38.27655136619493	31.19549588835878
5	12.511936041843489	37.79758880628709	35.6994730861268	13.991002065742626
6	29.92448434092071	38.60495369261736	17.509109383797917	13.961452582664016
7	26.169936153176458	33.296844626452206	22.592463729396954	17.940755490974382
8	24.747653215678937	36.249646956551906	20.471368338157507	18.531331489611656
9	24.525520423781742	16.894096632333095	22.188883172020567	36.391499771864595
10-11	23.48699363343903	27.3832406683271	28.79377059204383	20.335995106190033
12-13	24.330257506965896	24.866268958134334	29.20502281062967	21.598450724270094
14-15	21.57154641593222	26.932306385576304	27.049959505166992	24.446187693324482
16-17	20.307551000701814	30.225793774992955	27.947741285619205	21.51891393868603
18-19	20.184662109914495	28.44671367354425	30.939905856806355	20.428718359734898
20-21	21.33958987159444	27.649650943257022	30.72343294659705	20.28732623855149
22-23	20.962360843928522	27.51702827894199	30.8819950818556	20.63861579527389
24-25	20.8148268669753	27.60214054999445	30.84478799142289	20.738244591607362
26-27	20.53038066887985	27.651574894240667	30.86285312841495	20.95519130846453
28-29	20.554195586503482	27.62530764337987	31.07899694343504	20.741499826681608
30-31	20.92749628219418	27.960702122627985	30.592904760616534	20.5188968345613
32-33	20.604981402597094	27.735704146517676	30.959234567902833	20.7000798829824
34-35	20.77963254487179	27.526826771708578	30.845978825418303	20.847561858001328
36-37	21.07930850858656	27.64042845171647	30.454621587904647	20.825641451792322
38-39	20.85482982852767	27.728919067038515	30.640909445099833	20.775341659333975
40-41	20.777173213794313	27.512148448330358	30.732726629300366	20.977951708574963
42-43	20.770746593373424	27.552242016817164	30.53790230449677	21.13910908531264
44-45	20.925762114341293	27.684191601047832	30.310601865622665	21.07944441898821
46-47	21.057886440516935	27.882096561622166	29.901867512469455	21.158149485391444
48-49	21.016077812199786	27.76353738791599	29.7125702106663	21.50781458921793
50-51	21.009379370975594	27.835466350008375	29.458139466853268	21.697014812162767
52-53	20.92924400939309	27.967072922272973	29.45188111645347	21.65180195188047
54-55	21.030225437819176	28.208760447950326	29.30752485412931	21.453489260101186
56-57	20.886186299765537	27.89552580368998	29.28484723282539	21.933440663719097
58-59	20.783614553085787	27.58388748767824	29.59198763652635	22.040510322709626
60-61	20.91153682563524	27.788014204060797	29.326144579155383	21.974304391148582
62-63	20.66485944664015	27.734213100778987	29.449790685037613	22.15113676754325
64-65	20.611679648051602	27.581857540529132	29.513862731529837	22.29260007988943
66-67	20.563968625148497	27.59826386758547	29.329186383382794	22.508581123883236
68-69	20.880116986503772	27.782954957671997	29.038327059342045	22.298600996482186
70-71	20.77921834174295	27.489626153199758	29.015770525253576	22.715384979803716
72-73	20.533770628286604	27.53464485576541	29.148554987665808	22.783029528282178
74-75	20.657701498819907	27.53129887111526	28.95394423635052	22.85705539371431
76-77	20.678579925282932	27.53718184992955	28.82809767634634	22.956140548441184
78-79	20.703918646231838	27.526813062253343	28.824170552038524	22.945097739476296
80-81	20.56357451300868	27.38918700831813	28.835356399091804	23.211882079581382
82-83	20.40211200119705	27.313859394639305	29.01001901650196	23.274009587661688
84-85	20.382942442074338	27.180022258240637	28.804449266459205	23.63258603322582
86-87	20.549885718767985	27.258151323417827	28.66962614802222	23.52233680979197
88-89	20.448386681527072	27.110813805213535	28.723973715955324	23.716825797304068
90-91	20.242250677125302	26.983965802340897	28.653528656072126	24.120254864461682
92-93	20.283601558578898	26.958988121029652	28.58167184477895	24.175738475612498
94-95	20.193051035155666	26.729525007392606	28.761981796813735	24.315442160637996
96-97	20.19252549743854	26.73796396898758	28.701629811648893	24.36788072192499
98-99	20.21309595686435	26.67482302030716	28.58668845030942	24.525392572519074
100	20.235515840527494	26.63692569957395	28.529944942751982	24.597613517146577
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	540.0
1	418.5
2	609.5
3	996.5
4	737.5
5	472.5
6	1064.0
7	2685.0
8	4517.0
9	5412.0
10	4767.0
11	3841.0
12	3963.5
13	4530.0
14	5181.5
15	5895.0
16	6623.5
17	7505.0
18	8715.5
19	9995.0
20	11687.0
21	14264.0
22	18596.0
23	25129.5
24	32753.5
25	42137.5
26	55369.0
27	73162.5
28	96726.0
29	119145.0
30	136099.5
31	155120.0
32	174894.0
33	193607.0
34	212748.0
35	231065.5
36	245837.0
37	258514.5
38	269915.5
39	276470.0
40	283013.0
41	284960.5
42	281575.0
43	281601.0
44	280412.5
45	276028.5
46	270831.0
47	264734.0
48	256014.5
49	250315.0
50	244609.5
51	230856.5
52	214918.5
53	202558.5
54	193562.0
55	182157.0
56	165491.5
57	145935.5
58	128848.5
59	114697.0
60	97534.0
61	79532.5
62	64765.0
63	51478.5
64	40090.5
65	30908.0
66	23456.5
67	17802.0
68	13467.5
69	9934.5
70	7081.0
71	5070.5
72	3648.0
73	2360.5
74	1527.5
75	967.5
76	568.5
77	358.0
78	208.5
79	140.5
80	94.0
81	45.0
82	26.0
83	16.5
84	10.0
85	6.5
86	4.0
87	2.0
88	2.0
89	2.0
90	2.0
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05923104742392835
2	1.9415771664312183E-4
3	1.2943847776208121E-5
4	0.1187339156511571
5	0.0016438686675784313
6	0.0016568125153546395
7	0.0
8	0.0
9	3.883154332862436E-5
10-11	0.0
12-13	4.5303467216728423E-5
14-15	1.2943847776208121E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	6.471923888104061E-6
28-29	1.5532617331449745E-4
30-31	2.4593310774795427E-4
32-33	3.23596194405203E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.2651733608364212E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	6.471923888104061E-6
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.6179809720260152E-4
80-81	3.4948388995761925E-4
82-83	2.5240503163605834E-4
84-85	0.0
86-87	0.0
88-89	3.23596194405203E-5
90-91	3.883154332862436E-5
92-93	6.536643126985101E-4
94-95	6.795520082509264E-4
96-97	0.0
98-99	4.5303467216728423E-5
100	0.001074339365425274
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7725678.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.203466352009556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35539997147505	45.20504353005455
2	14.249783063119612	16.872731042016753
3	4.344699387323634	7.71663791961034
4	1.8079041270050888	4.2813676460322
5	0.9219187059251726	2.729039154276457
6	0.5385112356021838	1.9129039090291793
7	0.34355287890855213	1.4237664914618935
8	0.23134500921083156	1.0957141174815048
9	0.18565100843280533	0.989206490787141
>10	0.9248185134233762	9.816971231216026
>50	0.06257918152859622	2.5396950314527085
>100	0.031276348653849054	3.40177857451517
>500	0.001750968802543813	0.659003975467297
>1k	7.220762081154013E-4	0.6375880681081122
>5k	2.1881095167484668E-5	0.08238977294297371
>10k+	6.5643285502454E-5	0.6361630455477285
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19538	0.2528968978515542	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19050	0.24658030013676469	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10520	0.13616927860570943	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.2943847776208121E-5	0.0
5	0.0	0.0	0.0	2.5887695552416242E-5	0.0
6	0.0	0.0	0.0	3.883154332862436E-5	0.0
7	0.0	0.0	0.0	6.47192388810406E-5	0.0
8	0.0	0.0	0.0	6.47192388810406E-5	0.0
9	0.0	0.0	0.0	9.060693443345685E-5	0.0
10-11	6.471923888104061E-6	0.0	0.0	1.1649462998587308E-4	0.0
12-13	1.941577166431218E-5	0.0	0.0	1.4238232553828934E-4	0.0
14-15	2.5887695552416242E-5	0.0	0.0	2.0062964053122587E-4	0.0
16-17	2.5887695552416242E-5	0.0	0.0	2.394611838598502E-4	0.0
18-19	2.5887695552416242E-5	0.0	0.0	2.912365749646827E-4	0.0
20-21	2.5887695552416242E-5	0.0	0.0	3.8184350939813954E-4	0.0
22-23	3.23596194405203E-5	0.0	0.0	6.924958560271344E-4	0.0
24-25	6.47192388810406E-5	0.0	1.2943847776208121E-5	0.0014044074837185811	0.0
26-27	6.47192388810406E-5	0.0	1.2943847776208121E-5	0.002802343043549058	0.0
28-29	7.766308665724872E-5	0.0	1.2943847776208121E-5	0.009287210779429327	0.0
30-31	8.413501054535278E-5	0.0	1.2943847776208121E-5	0.02993264798248128	0.0
32-33	9.060693443345685E-5	0.0	1.2943847776208121E-5	0.07934578686815578	0.0
34-35	1.0355078220966497E-4	0.0	1.2943847776208121E-5	0.16396619170511637	0.0
36-37	1.0355078220966497E-4	0.0	1.2943847776208121E-5	0.30463345741305814	0.0
38-39	1.0355078220966497E-4	0.0	1.2943847776208121E-5	0.4978139135490762	0.0
40-41	1.0355078220966497E-4	0.0	1.2943847776208121E-5	0.704352420590141	0.0
42-43	1.294384777620812E-4	0.0	1.2943847776208121E-5	0.9183206962547494	0.0
44-45	1.3591040165018527E-4	0.0	1.2943847776208121E-5	1.188329360866451	0.0
46-47	1.4238232553828934E-4	0.0	1.2943847776208121E-5	1.5153233153129084	0.0
48-49	1.4238232553828934E-4	0.0	1.2943847776208121E-5	1.8619983903030906	0.0
50-51	1.4238232553828934E-4	0.0	1.2943847776208121E-5	2.21274430541889	6.471923888104061E-6
52-53	1.4238232553828934E-4	0.0	1.2943847776208121E-5	2.5589404580413526	1.2943847776208121E-5
54-55	1.4238232553828934E-4	0.0	1.2943847776208121E-5	2.9471135090020577	1.2943847776208121E-5
56-57	1.4238232553828934E-4	0.0	1.2943847776208121E-5	3.40909885190659	1.2943847776208121E-5
58-59	1.4238232553828934E-4	0.0	1.2943847776208121E-5	3.908330116787161	1.2943847776208121E-5
60-61	1.4238232553828934E-4	0.0	1.2943847776208121E-5	4.416026658113372	1.2943847776208121E-5
62-63	1.4238232553828934E-4	0.0	1.2943847776208121E-5	4.91294744616589	1.2943847776208121E-5
64-65	1.4238232553828934E-4	0.0	1.2943847776208121E-5	5.439224880974848	1.2943847776208121E-5
66-67	1.4238232553828934E-4	0.0	1.2943847776208121E-5	6.0388680449793535	1.2943847776208121E-5
68-69	1.4238232553828934E-4	0.0	1.2943847776208121E-5	6.690241296621474	1.2943847776208121E-5
70-71	1.4238232553828934E-4	0.0	1.2943847776208121E-5	7.362590053584941	1.2943847776208121E-5
72-73	1.4238232553828934E-4	0.0	1.2943847776208121E-5	8.044892629488313	1.2943847776208121E-5
74-75	1.4238232553828934E-4	0.0	1.2943847776208121E-5	8.722962567168862	1.2943847776208121E-5
76-77	1.5532617331449745E-4	0.0	1.2943847776208121E-5	9.43486772293642	1.2943847776208121E-5
78-79	1.5532617331449745E-4	0.0	1.2943847776208121E-5	10.211828657627201	1.2943847776208121E-5
80-81	1.5532617331449745E-4	0.0	1.2943847776208121E-5	11.023226699326582	1.2943847776208121E-5
82-83	1.5532617331449745E-4	0.0	1.2943847776208121E-5	11.829686663099341	1.2943847776208121E-5
84-85	1.5532617331449745E-4	0.0	1.2943847776208121E-5	12.61170864227062	1.2943847776208121E-5
86-87	1.5532617331449745E-4	0.0	1.2943847776208121E-5	13.424109832172658	1.2943847776208121E-5
88	1.5532617331449745E-4	0.0	1.2943847776208121E-5	14.059736892995023	1.2943847776208121E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14250	0.0	56.44446	1
GTATCAA	25485	0.0	39.839367	1
GTGGTAT	5805	0.0	33.098343	1
ATCAACG	31680	0.0	31.880314	3
TCAACGC	32005	0.0	31.571283	4
TATCAAC	32525	0.0	31.023123	2
CAACGCA	33310	0.0	30.420158	5
TGGTATC	6395	0.0	29.069302	2
AACGCAG	35345	0.0	28.734823	6
CGCAGAG	39760	0.0	25.188643	8
ACGCAGA	40420	0.0	24.998264	7
GCAGAGT	42920	0.0	22.939926	9
GTACATG	23555	0.0	22.431488	1
TACATGG	23895	0.0	21.468294	2
GAGTACT	29075	0.0	21.320272	12-13
CAGAGTA	42150	0.0	20.432152	10-11
ACATGGG	24270	0.0	20.147623	3
AGAGTAC	40005	0.0	20.02398	10-11
GTACTTT	31440	0.0	19.589445	14-15
AGTACTT	30040	0.0	19.06309	12-13
>>END_MODULE