FastQCFastQC Report
Wed 25 May 2016
SRR2049522_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049522_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8177053
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75350.042.3157581
GTATCAA118950.035.3675271
ATCAACG124650.032.3488733
TCAACGC124950.032.1483464
CAACGCA132750.030.2594035
TATCAAC143150.028.4899832
AACGCAG147300.027.582336
GTACATG210250.023.2468831
ACGCAGA174650.022.8223887
CGCAGAG176350.022.0114528
TACATGG214600.021.8657722
ACATGGG218700.020.5667343
GTGGTAT45550.020.3370291
GCAGAGT205450.017.9028139
GAGTACA170200.017.4708441
CATGGGG154900.016.847834
AGAGTAC173700.016.20046210-11
TGGTATC54400.015.8969212
CAGAGTA192850.015.54186410-11
AGTACAT172600.015.3869242
Produced by FastQC (version 0.11.5)