##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049522_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8177053 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.79232664873274 31.0 31.0 34.0 28.0 34.0 2 30.97366055961726 31.0 31.0 34.0 28.0 34.0 3 31.066292954197557 31.0 31.0 34.0 28.0 34.0 4 34.72095973940734 37.0 35.0 37.0 32.0 37.0 5 34.7490457748042 37.0 35.0 37.0 32.0 37.0 6 34.834871316108625 37.0 35.0 37.0 32.0 37.0 7 30.901598167457152 37.0 33.0 37.0 2.0 37.0 8 32.658358946676756 37.0 33.0 37.0 17.0 37.0 9 35.50488947546261 39.0 35.0 39.0 30.0 39.0 10-11 36.04852078126435 39.0 36.0 39.0 32.0 39.0 12-13 36.212010671815385 39.0 37.0 39.0 32.0 39.0 14-15 37.423436536365855 40.0 38.0 41.0 32.0 41.0 16-17 37.43315929345205 40.0 38.0 41.0 32.0 41.0 18-19 37.422442535226324 40.0 38.0 41.0 32.0 41.0 20-21 37.49285665630393 40.0 38.0 41.0 32.0 41.0 22-23 37.45612190602165 40.0 38.0 41.0 32.0 41.0 24-25 37.43143104245503 40.0 38.0 41.0 31.5 41.0 26-27 37.45842083939043 40.0 38.0 41.0 32.0 41.0 28-29 37.44152783404975 40.0 38.0 41.0 32.0 41.0 30-31 37.36923124993809 40.0 37.0 41.0 31.0 41.0 32-33 37.325143606137814 40.0 37.0 41.0 31.0 41.0 34-35 37.27321432305746 40.0 37.0 41.0 31.0 41.0 36-37 37.10645931975738 40.0 37.0 41.0 30.5 41.0 38-39 37.003390035505454 40.0 37.0 41.0 30.0 41.0 40-41 36.928173939926765 40.0 37.0 41.0 30.0 41.0 42-43 36.814453813617206 40.0 37.0 41.0 30.0 41.0 44-45 36.699314227265006 40.0 36.5 41.0 30.0 41.0 46-47 36.54481675733299 40.0 36.0 41.0 30.0 41.0 48-49 36.46434870851394 40.0 36.0 41.0 30.0 41.0 50-51 36.32568793427167 40.0 36.0 41.0 30.0 41.0 52-53 36.205314799842924 39.0 35.0 41.0 29.5 41.0 54-55 36.08142157082753 39.0 35.0 41.0 29.0 41.0 56-57 35.88026059021508 39.0 35.0 41.0 29.0 41.0 58-59 35.690619285456506 39.0 35.0 41.0 28.5 41.0 60-61 35.46517412813638 39.0 35.0 40.0 28.0 41.0 62-63 35.22150082676485 38.0 34.5 40.0 28.0 41.0 64-65 34.85705192322955 37.5 34.0 40.0 27.5 41.0 66-67 31.342273677326048 36.0 31.0 40.0 6.5 41.0 68-69 31.70282625048413 36.0 30.5 39.0 11.0 41.0 70-71 31.85928439010974 35.5 30.0 39.0 15.5 41.0 72-73 33.10034587032761 35.0 32.5 39.0 25.5 40.5 74-75 33.18377170846269 35.0 33.0 38.5 26.0 40.0 76-77 32.88461509299255 35.0 33.0 37.0 25.5 39.5 78-79 29.515366660825116 35.0 29.0 36.5 6.5 39.0 80-81 31.303198841929973 35.0 30.5 36.0 21.5 39.0 82-83 31.70549934065488 35.0 32.0 36.0 23.5 37.5 84-85 29.859459820059868 35.0 30.5 35.5 12.5 37.0 86-87 29.495272074181248 35.0 29.0 35.0 13.5 37.0 88-89 30.745698542005293 35.0 30.5 35.0 20.5 36.0 90-91 30.897115073119863 35.0 32.0 35.0 20.0 36.0 92-93 30.746850607425436 35.0 32.0 35.0 19.5 36.0 94-95 30.53985213254702 35.0 32.0 35.0 15.5 36.0 96-97 30.347810146271524 35.0 32.0 35.0 6.0 35.0 98-99 30.158202472210952 35.0 32.0 35.0 2.0 35.0 100 29.943888831343028 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.33996030489257123 1101 2 0.31851957004342424 1101 3 0.3509755286248897 1101 4 0.3774970047731756 1101 5 0.3586039402145218 1101 6 0.3589360567314017 1101 7 -12.150907522316118 1101 8 -6.532921875271079 1101 9 -2.218618364460852 1101 10-11 -0.12963371557753334 1101 12-13 0.39677599089377935 1101 14-15 0.5959292218660082 1101 16-17 0.6388327042694115 1101 18-19 0.632880676003289 1101 20-21 0.6377229975336363 1101 22-23 0.574189210111065 1101 24-25 0.6246987059723281 1101 26-27 0.5814138232149801 1101 28-29 0.5927090824213295 1101 30-31 0.6560067892697816 1101 32-33 0.674628827518923 1101 34-35 0.6801182023128689 1101 36-37 0.7121281876400118 1101 38-39 0.6903966826646126 1101 40-41 0.7019906442753481 1101 42-43 0.6519236043218228 1101 44-45 0.6290612976422167 1101 46-47 0.6037831223629198 1101 48-49 0.5468072229959589 1101 50-51 0.6336378756485246 1101 52-53 0.6658043462876364 1101 54-55 0.5725762696419991 1101 56-57 0.5399460366205915 1101 58-59 0.5049773988439554 1101 60-61 0.513330958299349 1101 62-63 0.4999918562951038 1101 64-65 0.3883587111953162 1101 66-67 -10.271273904241337 1101 68-69 -8.160623192884739 1101 70-71 -6.671414889041454 1101 72-73 -1.457078362865186 1101 74-75 -0.20418878574616883 1101 76-77 0.05269746403654807 1101 78-79 3.180530584453649 1101 80-81 1.0732814799602863 1101 82-83 0.3898848670801449 1101 84-85 -5.385419742149079 1101 86-87 -5.89292328545184 1101 88-89 -1.2783743213612517 1101 90-91 -0.00805262852830424 1101 92-93 0.3460458705795766 1101 94-95 0.3916227036798787 1101 96-97 0.5021361367290744 1101 98-99 0.487291108458372 1101 100 0.5155228297194085 1102 1 0.21954877091674518 1102 2 0.22950673704325908 1102 3 0.17548108934148132 1102 4 0.2745270002409228 1102 5 0.2778607444565466 1102 6 0.33856962152014347 1102 7 -12.330837935838296 1102 8 -6.696399897774505 1102 9 -2.3118824511127514 1102 10-11 -0.1986868319287325 1102 12-13 0.33670239025069293 1102 14-15 0.5487011132190673 1102 16-17 0.5576828249076158 1102 18-19 0.5688841284091524 1102 20-21 0.5236113305128427 1102 22-23 0.5021417604588976 1102 24-25 0.5455150194803124 1102 26-27 0.4829114536479082 1102 28-29 0.4241757030546722 1102 30-31 0.48798556322895337 1102 32-33 0.527365210382662 1102 34-35 0.523112240564295 1102 36-37 0.6599686499271655 1102 38-39 0.6301420087688072 1102 40-41 0.5400970797369027 1102 42-43 0.4850089305737768 1102 44-45 0.4120419104405286 1102 46-47 0.4783178046552763 1102 48-49 0.427938406564806 1102 50-51 0.484723006552521 1102 52-53 0.48097213683434603 1102 54-55 0.448372649943515 1102 56-57 0.45911672648345814 1102 58-59 0.4070490474523538 1102 60-61 0.4151708089063959 1102 62-63 0.4075914071392859 1102 64-65 0.3337371412423167 1102 66-67 -10.435866065026836 1102 68-69 -6.774942023834154 1102 70-71 -6.326123426360407 1102 72-73 -1.0797918425668627 1102 74-75 -0.11515059619809165 1102 76-77 0.10935096044698156 1102 78-79 3.2818340252136835 1102 80-81 1.1747069491071116 1102 82-83 0.5690859302652775 1102 84-85 -5.4599950625044755 1102 86-87 -5.959676600197053 1102 88-89 -1.3017117958756472 1102 90-91 0.043307525367300315 1102 92-93 0.39727562935979677 1102 94-95 0.3919685012713785 1102 96-97 0.4499066354529866 1102 98-99 0.5386262786964764 1102 100 0.6421476268524593 1103 1 0.09919965982416912 1103 2 0.10524495911086618 1103 3 0.10419091395357327 1103 4 0.1532302543207038 1103 5 0.1681309403728335 1103 6 0.21854833580042765 1103 7 -12.197290325013185 1103 8 -6.662965471965229 1103 9 -2.328366306047066 1103 10-11 -0.3189133034736358 1103 12-13 0.23492675926976858 1103 14-15 0.38509089611812897 1103 16-17 0.40803571803554917 1103 18-19 0.4355909017029944 1103 20-21 0.40175963451742547 1103 22-23 0.37969753668699013 1103 24-25 0.4552469028041273 1103 26-27 0.45226034895809875 1103 28-29 0.4007307570473557 1103 30-31 0.39794537267528085 1103 32-33 0.44521180685249107 1103 34-35 0.4001068334491933 1103 36-37 0.43372556543189944 1103 38-39 0.4913668677450147 1103 40-41 0.4095314977100699 1103 42-43 0.3663985784558079 1103 44-45 0.352552480092875 1103 46-47 0.38732560485553336 1103 48-49 0.3152564154533053 1103 50-51 0.35019046146935295 1103 52-53 0.2899726285278845 1103 54-55 0.296543765705394 1103 56-57 0.34073857472889557 1103 58-59 0.29460212845987854 1103 60-61 0.29043103949680216 1103 62-63 0.22127754655740972 1103 64-65 0.09104261480628395 1103 66-67 -10.394805180179269 1103 68-69 -8.342704659297258 1103 70-71 -6.822334745899383 1103 72-73 -1.6833768354131848 1103 74-75 -0.4696970205429096 1103 76-77 -0.18447701119619353 1103 78-79 2.972647548386533 1103 80-81 0.8558840848478937 1103 82-83 0.16419176414330394 1103 84-85 -5.6172137645257045 1103 86-87 -6.075594659169866 1103 88-89 -1.5347831249199153 1103 90-91 -0.24953094837518108 1103 92-93 0.1389532092543213 1103 94-95 0.16956891660292683 1103 96-97 0.2698746734809063 1103 98-99 0.27555292851506863 1103 100 0.3382284840924328 1104 1 0.11370820633831613 1104 2 0.09815715423736648 1104 3 0.11420452806492065 1104 4 0.15552462152980695 1104 5 0.19309075191956282 1104 6 0.19195187296064375 1104 7 -12.257987581434545 1104 8 -6.672261130917288 1104 9 -2.3907484814350966 1104 10-11 -0.3195272331089427 1104 12-13 0.18978012682357104 1104 14-15 0.39471850656656216 1104 16-17 0.3733633614619407 1104 18-19 0.3972961388599998 1104 20-21 0.3653325848554516 1104 22-23 0.3874681130182722 1104 24-25 0.47608929956915347 1104 26-27 0.44666914920171763 1104 28-29 0.388460925835048 1104 30-31 0.3593542234325682 1104 32-33 0.40339804879414487 1104 34-35 0.3794406624266955 1104 36-37 0.43499104509519526 1104 38-39 0.464554545851243 1104 40-41 0.5027283979507473 1104 42-43 0.4295939200616843 1104 44-45 0.4047219213244162 1104 46-47 0.40358721293439714 1104 48-49 0.4035685338939601 1104 50-51 0.43576252069883026 1104 52-53 0.4214447557050178 1104 54-55 0.3618358376785551 1104 56-57 0.41810308474165936 1104 58-59 0.4287808580191168 1104 60-61 0.3820197235708491 1104 62-63 0.2983974763434105 1104 64-65 0.2217170328629905 1104 66-67 -10.373562170198667 1104 68-69 -8.322766804668642 1104 70-71 -6.779810745338988 1104 72-73 -1.6399182048776915 1104 74-75 -0.39485533342710255 1104 76-77 -0.09162434125647678 1104 78-79 3.084046266746313 1104 80-81 0.977540933979931 1104 82-83 0.3519590638665697 1104 84-85 -5.468715798166958 1104 86-87 -5.98401804201422 1104 88-89 -1.439399580408093 1104 90-91 -0.12852072291725847 1104 92-93 0.22465457203000128 1104 94-95 0.25293499694661037 1104 96-97 0.3661555771873637 1104 98-99 0.39256351612885965 1104 100 0.5079901723066556 1105 1 0.0666517027733704 1105 2 0.10969601909161852 1105 3 0.08528739674994412 1105 4 0.1313597928017316 1105 5 0.15266958756886595 1105 6 0.23026100693897433 1105 7 -12.193636550359756 1105 8 -6.6897139089524025 1105 9 -2.4309657472457076 1105 10-11 -0.392647011544355 1105 12-13 0.15624409267584127 1105 14-15 0.33935694658670457 1105 16-17 0.37793156464771016 1105 18-19 0.34408071795434125 1105 20-21 0.3571876835126062 1105 22-23 0.330482677362653 1105 24-25 0.3831203241680754 1105 26-27 0.36809753934188194 1105 28-29 0.31142715094831885 1105 30-31 0.34128932201802087 1105 32-33 0.3502683121251309 1105 34-35 0.35372807878653845 1105 36-37 0.4375618471827565 1105 38-39 0.43011411677538547 1105 40-41 0.4167712815006084 1105 42-43 0.35980443012905283 1105 44-45 0.3019700630367126 1105 46-47 0.28543053477037716 1105 48-49 0.27185344814779455 1105 50-51 0.36708399263689273 1105 52-53 0.35253133693229444 1105 54-55 0.3842658788876463 1105 56-57 0.3066965380219244 1105 58-59 0.30970396802223377 1105 60-61 0.2722082704452333 1105 62-63 0.20807047400882794 1105 64-65 0.06339066181946151 1105 66-67 -10.350971861885334 1105 68-69 -8.34680984424875 1105 70-71 -6.791012525778594 1105 72-73 -1.6956303644251811 1105 74-75 -0.49775320017987923 1105 76-77 -0.21445421711424473 1105 78-79 2.927874345526682 1105 80-81 0.8235768204488991 1105 82-83 0.16033325023806455 1105 84-85 -5.564168394261834 1105 86-87 -6.10486481615192 1105 88-89 -1.5695034602978772 1105 90-91 -0.2854727444704537 1105 92-93 0.0772809950320088 1105 94-95 0.10034797260157191 1105 96-97 0.16345120831588034 1105 98-99 0.21598456942512811 1105 100 0.2924019109635623 1106 1 0.1989279058751947 1106 2 0.22094581931677837 1106 3 0.21590004253576467 1106 4 0.23222846100897954 1106 5 0.2180445059482068 1106 6 0.26598063421408114 1106 7 -12.287208580699598 1106 8 -6.713076556314213 1106 9 -2.467903925110363 1106 10-11 -0.28877168175867496 1106 12-13 0.2519018523814225 1106 14-15 0.41426492930682457 1106 16-17 0.4154661192034581 1106 18-19 0.4546035157708985 1106 20-21 0.4366297165050028 1106 22-23 0.44211445786285 1106 24-25 0.5116676712708781 1106 26-27 0.4538093291392542 1106 28-29 0.4735801751613451 1106 30-31 0.42298156525374253 1106 32-33 0.4673524130789062 1106 34-35 0.49216149519158847 1106 36-37 0.5423995323494921 1106 38-39 0.5062588400885772 1106 40-41 0.5035731845884825 1106 42-43 0.49847404874356727 1106 44-45 0.41775488689042817 1106 46-47 0.49897618519561604 1106 48-49 0.4754041505533593 1106 50-51 0.5463231788365803 1106 52-53 0.5049741851595613 1106 54-55 0.4734557155979857 1106 56-57 0.45068353659524263 1106 58-59 0.43748822994234615 1106 60-61 0.4372084042000637 1106 62-63 0.3789786059333551 1106 64-65 0.2866431860050014 1106 66-67 -10.389196535833033 1106 68-69 -8.258483070781384 1106 70-71 -6.752742182477473 1106 72-73 -1.5225667971077783 1106 74-75 -0.26691524854869186 1106 76-77 0.03694983786835593 1106 78-79 3.158238520894514 1106 80-81 1.0235557855013937 1106 82-83 0.32723030510696915 1106 84-85 -5.52795615399322 1106 86-87 -5.9726270834866995 1106 88-89 -1.378815340859095 1106 90-91 -0.11652717314838057 1106 92-93 0.23123833334944877 1106 94-95 0.32238467355487543 1106 96-97 0.400816620989378 1106 98-99 0.47059649636448597 1106 100 0.5768828068380571 1107 1 0.09489040996146514 1107 2 0.08032240805616198 1107 3 0.07736866052039204 1107 4 0.07952385064714917 1107 5 0.12086682536492788 1107 6 0.10090905750838175 1107 7 -12.285442191566322 1107 8 -6.768058159785184 1107 9 -2.5278336185202335 1107 10-11 -0.4920977728054652 1107 12-13 0.1303487445575442 1107 14-15 0.24079489299727896 1107 16-17 0.2540624960980793 1107 18-19 0.31643277650057655 1107 20-21 0.2555058411704394 1107 22-23 0.30109484062137426 1107 24-25 0.32507539658674744 1107 26-27 0.287408353129905 1107 28-29 0.27587545793461743 1107 30-31 0.33366010128174395 1107 32-33 0.32093818582246314 1107 34-35 0.2490843608105564 1107 36-37 0.33659589256618005 1107 38-39 0.3567951792336146 1107 40-41 0.3537156652766029 1107 42-43 0.34336704544897856 1107 44-45 0.24169455061988998 1107 46-47 0.30771917021596096 1107 48-49 0.2860488051537544 1107 50-51 0.3144123048604257 1107 52-53 0.2917724813477136 1107 54-55 0.27692665237566416 1107 56-57 0.26320603515957686 1107 58-59 0.2071658588450589 1107 60-61 0.18645773962303736 1107 62-63 0.13422383283906214 1107 64-65 0.06994524821718784 1107 66-67 -10.453166948622346 1107 68-69 -8.41722964716298 1107 70-71 -6.8901126263101595 1107 72-73 -1.7734125539232188 1107 74-75 -0.5424650509187074 1107 76-77 -0.29560485330785724 1107 78-79 2.9297655081348495 1107 80-81 0.8111004191142186 1107 82-83 0.13919293673036393 1107 84-85 -5.658313492685913 1107 86-87 -6.143463583349526 1107 88-89 -1.6300515574773904 1107 90-91 -0.3718166876699165 1107 92-93 0.025309188629503154 1107 94-95 0.11096880478356752 1107 96-97 0.11198116454838569 1107 98-99 0.18874327687554882 1107 100 0.26999282407308556 1108 1 0.045856580115614776 1108 2 0.078756907443708 1108 3 0.0785466568612101 1108 4 0.039202308307601186 1108 5 0.04007873712400567 1108 6 0.06775694664171539 1108 7 -12.361413828777707 1108 8 -6.780634614895959 1108 9 -2.615081231302753 1108 10-11 -0.49057160208786854 1108 12-13 0.0581838871529925 1108 14-15 0.20402217728285166 1108 16-17 0.25156460214951437 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-0.6429744773495543 2216 6 -0.9653552212396477 2216 7 3.1098023955787326 2216 8 1.25236683011844 2216 9 -0.07522500814463484 2216 10-11 -0.7226475755496722 2216 12-13 -1.1058045474018812 2216 14-15 -1.2720758641113008 2216 16-17 -1.474217455779069 2216 18-19 -1.8786451528579846 2216 20-21 -1.4414071963988917 2216 22-23 -1.2404852443040255 2216 24-25 -1.3290436742531355 2216 26-27 -1.8012758669471793 2216 28-29 -1.9874596746001174 2216 30-31 -1.8765175100119862 2216 32-33 -2.767114286766734 2216 34-35 -1.797575282793126 2216 36-37 -1.468150311929655 2216 38-39 -1.7686139846473878 2216 40-41 -1.7844896429080706 2216 42-43 -1.426560847500852 2216 44-45 -1.538663980799349 2216 46-47 -1.7740050168423025 2216 48-49 -1.8294395583374339 2216 50-51 -2.3760239937575847 2216 52-53 -2.1085066844405915 2216 54-55 -1.7585652014118125 2216 56-57 -1.7470319420438472 2216 58-59 -2.107177116045655 2216 60-61 -2.241065524514397 2216 62-63 -2.1359428596959518 2216 64-65 -1.850725896376055 2216 66-67 1.5741676889389637 2216 68-69 0.7726474897747799 2216 70-71 0.41271072994947744 2216 72-73 -1.3258568616901343 2216 74-75 -2.1770060306263446 2216 76-77 -1.791544970788756 2216 78-79 0.7100670905826689 2216 80-81 -0.7286400209874309 2216 82-83 -1.6230453007009018 2216 84-85 0.209459586319106 2216 86-87 0.19808343256298144 2216 88-89 -1.1081483074092482 2216 90-91 -1.335316354470173 2216 92-93 -1.4476916901220847 2216 94-95 -1.453034599799686 2216 96-97 -1.448650571356648 2216 98-99 -1.4786283987523738 2216 100 -2.680842839341892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104378.0 3 19754.0 4 11785.0 5 11060.0 6 10178.0 7 11400.0 8 12652.0 9 14113.0 10 16216.0 11 18363.0 12 20541.0 13 23062.0 14 26451.0 15 29271.0 16 31109.0 17 32354.0 18 30343.0 19 28851.0 20 28031.0 21 28374.0 22 30525.0 23 34870.0 24 42014.0 25 52974.0 26 66178.0 27 81178.0 28 101376.0 29 129936.0 30 160974.0 31 200174.0 32 269243.0 33 382980.0 34 586991.0 35 807030.0 36 911867.0 37 1535795.0 38 1716829.0 39 556507.0 40 1326.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.16146853268313 20.36459389580802 14.412599402952358 23.061338168556485 2 15.55894615678503 20.30886703097965 40.41075954413753 23.7214272680978 3 18.418532707475585 25.011648614677036 30.449539913351426 26.120278764495954 4 12.81258144162515 17.137170719012378 38.56751282183926 31.48273501752321 5 13.274637152009397 37.503780462764105 35.40731589406217 13.814266491164325 6 30.784230035918654 37.67196498389959 17.89756641037275 13.646238569809007 7 27.14699409692202 32.46297315461212 22.449179158326945 17.940853590138918 8 24.707449579628452 36.311350349734845 20.723312020028732 18.25788805060797 9 24.601454827307588 17.415809827819388 21.843370710694916 36.13936463417811 10-11 23.63199798264729 27.17688756572814 29.0249494530609 20.166164998563662 12-13 24.082264111532602 24.672372797388007 28.951377715174402 22.293985375904988 14-15 21.638590333216627 27.049940852774217 26.079848082188047 25.23162073182111 16-17 21.23242698936582 30.231607376176285 26.831405004361 21.7045606300969 18-19 21.178644677978728 29.30284908267074 28.586325660357097 20.932180578993435 20-21 22.253984550056394 28.569591549033102 28.129243391796738 21.047180509113765 22-23 21.9966913505814 28.407748391000304 28.153598133312705 21.441962125105594 24-25 21.807661024087775 28.45551447446898 28.137906162525788 21.598918338917457 26-27 21.543161472406023 28.467948907660695 28.175141169699835 21.813748450233447 28-29 21.56179004832181 28.50917072446516 28.324287490860094 21.604751736352938 30-31 21.898573972799245 28.84401018313077 27.866157893314377 21.39125795075561 32-33 21.545582686767798 28.63693419095292 28.264599515815725 21.552883606463553 34-35 21.71435164794445 28.355230287497918 28.25181280218338 21.67860526237425 36-37 21.99043127034046 28.58195716500274 27.82321795189758 21.60439361275922 38-39 21.762558408893376 28.67275133786007 28.074277633831564 21.490412619414993 40-41 21.667543306861287 28.436987017205347 28.20889750867458 21.68657216725879 42-43 21.610234446976396 28.443278118035575 28.170288520256964 21.77619891473106 44-45 21.741934150475288 28.579667671798475 28.049438934910043 21.628959242816194 46-47 21.722090025262986 28.673603006977206 27.93709660012113 21.667210367638674 48-49 21.649229861619865 28.488721022472223 28.045410761361328 21.816638354546583 50-51 21.630453219733774 28.409035459953845 28.05932400614551 21.901187314166872 52-53 21.655708844605115 28.450469610899944 28.05300894193256 21.840812602562384 54-55 21.755634520690375 28.719003173097214 27.86783802155955 21.65752428465286 56-57 21.60195506053706 28.562314955844997 27.855778697478705 21.979951286139237 58-59 21.512119535398295 28.23698544171105 28.18645973404388 22.06443528884678 60-61 21.77976319541032 28.495895720569752 27.891098310091788 21.833242773928138 62-63 21.56096715043593 28.577328491299202 27.93456393106088 21.92714042720399 64-65 21.615977890110823 28.368029030786385 28.023121510284767 21.992871568818025 66-67 21.47989710608261 28.347544248901922 27.9666573548433 22.205901290172168 68-69 21.938773926498914 28.468530759445017 27.785100346568537 21.807594967487535 70-71 21.678595562461773 28.240557324403575 27.954713876187213 22.12613323694744 72-73 21.482990010957618 28.347784506711545 28.141998982506944 22.027226499823897 74-75 21.492842818395705 28.38276900243151 28.039860718504084 22.084527460668703 76-77 21.52264953567206 28.3490239375095 27.89053888824851 22.23778763856993 78-79 21.684053771673018 28.36504550400521 27.978617755045253 21.972282969276517 80-81 21.43683578980235 28.262066899927728 28.076478025818684 22.22461928445124 82-83 21.377547716454657 28.244788933085825 28.234956067944854 22.142707282514664 84-85 21.47212743981976 28.132800265750596 28.063584646049144 22.3314876483805 86-87 21.54759700487504 28.1917616697286 28.044472170741592 22.216169154654768 88-89 21.37201677061984 28.072982011928943 28.180332959150018 22.3746682583012 90-91 21.31164943302618 27.997611776310467 28.166192631740444 22.524546158922906 92-93 21.451969214420814 28.098325856952588 28.024164367006183 22.425540561620412 94-95 21.36535329175153 27.855879123368766 28.199818423824137 22.578949161055572 96-97 21.311038648704805 27.840304901412004 28.258554959166826 22.590101490716364 98-99 21.323128294723933 27.875326640383985 28.161147868717546 22.64039719617453 100 21.38177406473848 27.794899829880183 28.10734059063742 22.715985514743924 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2554.0 1 2029.5 2 1786.5 3 2079.5 4 1927.5 5 1688.0 6 1564.0 7 1527.5 8 1605.0 9 1696.0 10 1568.5 11 1406.5 12 1436.0 13 1523.5 14 1663.5 15 1883.0 16 2256.5 17 2837.5 18 3722.0 19 5215.0 20 7570.0 21 10918.5 22 16179.5 23 24254.5 24 34018.0 25 45523.0 26 61629.0 27 82075.0 28 106524.5 29 131473.0 30 151748.0 31 171691.5 32 192335.5 33 210404.5 34 228271.0 35 245442.0 36 259072.0 37 270786.5 38 280876.5 39 287411.0 40 292201.0 41 292907.5 42 288946.5 43 285786.5 44 281968.0 45 277133.0 46 272720.0 47 266736.5 48 260025.5 49 255115.5 50 249637.5 51 239018.5 52 226337.5 53 216520.0 54 208960.5 55 198799.0 56 182067.5 57 162901.0 58 146721.5 59 132127.0 60 114661.0 61 96281.5 62 80568.0 63 65744.0 64 52545.0 65 41814.5 66 32771.0 67 25309.0 68 19178.0 69 14506.5 70 10756.0 71 7908.0 72 5889.5 73 4203.0 74 2875.0 75 1920.0 76 1263.5 77 890.5 78 602.5 79 431.5 80 310.5 81 203.5 82 155.5 83 121.0 84 97.0 85 86.0 86 73.0 87 56.5 88 45.5 89 37.5 90 26.5 91 25.0 92 24.5 93 14.5 94 13.0 95 14.0 96 13.0 97 14.0 98 12.5 99 15.5 100 30.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.018233952990154276 2 0.0024580982904232125 3 0.0014063746437744749 4 0.0022501994300391593 5 0.001761025640900212 6 0.0029717307690191076 7 11.582363474958521 8 1.2229344728473693E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.057336182118423E-4 18-19 0.0 20-21 6.114672364236847E-6 22-23 6.114672364236847E-6 24-25 0.0 26-27 1.8344017092710542E-4 28-29 0.0 30-31 0.0 32-33 1.2229344728473693E-5 34-35 3.0573361821184234E-5 36-37 1.2229344728473693E-5 38-39 2.4458689456947387E-5 40-41 0.0 42-43 1.834401709271054E-5 44-45 1.956695156555791E-4 46-47 8.621688033573954E-4 48-49 5.931232193309742E-4 50-51 3.1796296294031606E-4 52-53 2.1401353274828964E-4 54-55 3.2407763530455287E-4 56-57 0.0014614066950526063 58-59 3.3630698003302655E-4 60-61 0.0012229344728473693 62-63 0.0022807727918603437 64-65 9.60003561185185E-4 66-67 5.791634223234214 68-69 5.445103511008184 70-71 0.0024397542733305016 72-73 0.0011373290597480536 74-75 0.0028249786322774233 76-77 0.005509319800177399 78-79 5.395531862151316 80-81 0.004616577634998819 82-83 0.004800017805925924 84-85 5.791689255285492 86-87 0.003656574073813634 88-89 0.0016387321936154747 90-91 0.003234661680681292 92-93 0.0036993767803632925 94-95 0.003907275640747345 96-97 0.0030084188032045286 98-99 0.005362567663435715 100 0.00566218660928332 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8177053.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.3800396788003 #Duplication Level Percentage of deduplicated Percentage of total 1 91.10167126726125 78.69365978871049 2 6.24149467142361 10.782811147451843 3 1.3713152859425912 3.5536280643559923 4 0.5394329122534949 1.863849454580308 5 0.25983564516588026 1.1222306669697706 6 0.14970746215107333 0.7759041912493325 7 0.09219537174665539 0.5574687908774486 8 0.06160504871809471 0.42571572421467574 9 0.04001800708366184 0.31110813357779 >10 0.14097066212694498 1.7996559263937797 >50 0.001460050036692087 0.0781220119510129 >100 2.9361609004698757E-4 0.03584609966747406 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.2229344728473693E-5 0.0 8 0.0 0.0 0.0 1.2229344728473693E-5 0.0 9 0.0 0.0 0.0 1.2229344728473693E-5 0.0 10-11 0.0 0.0 0.0 1.2229344728473693E-5 0.0 12-13 0.0 0.0 0.0 1.2229344728473693E-5 0.0 14-15 0.0 6.114672364236847E-6 0.0 1.834401709271054E-5 0.0 16-17 0.0 1.834401709271054E-5 0.0 4.8917378913894774E-5 0.0 18-19 0.0 2.4458689456947387E-5 0.0 4.8917378913894774E-5 0.0 20-21 0.0 2.4458689456947387E-5 0.0 8.560541309931585E-5 0.0 22-23 0.0 3.0573361821184234E-5 0.0 2.3235754984100016E-4 0.0 24-25 0.0 4.8917378913894774E-5 0.0 4.708297720462372E-4 0.0 26-27 0.0 6.114672364236847E-5 0.0 9.905769230063692E-4 0.0 28-29 0.0 6.114672364236847E-5 0.0 0.0028127492875489497 0.0 30-31 0.0 6.114672364236847E-5 0.0 0.008004106124786033 0.0 32-33 0.0 6.114672364236847E-5 0.0 0.01995217592450483 0.0 34-35 0.0 6.114672364236847E-5 0.0 0.04040575498287709 0.0 36-37 0.0 6.114672364236847E-5 0.0 0.07180459757323329 0.0 38-39 0.0 6.726139600660532E-5 0.0 0.11414870369557345 0.0 40-41 0.0 7.337606837084216E-5 0.0 0.161433465088217 0.0 42-43 0.0 8.560541309931585E-5 0.0 0.21185505340371402 0.0 44-45 0.0 8.560541309931585E-5 0.0 0.27471388530806884 0.0 46-47 0.0 9.172008546355271E-5 0.0 0.3520278026814795 0.0 48-49 0.0 9.783475782778955E-5 0.0 0.43518734683510063 0.0 50-51 0.0 1.0394943019202639E-4 0.0 0.5224620654898531 0.0 52-53 0.0 1.1006410255626324E-4 0.0 0.6108435398425325 0.0 54-55 0.0 1.1006410255626324E-4 0.0 0.7105555020861427 0.0 56-57 0.0 1.1006410255626324E-4 0.0 0.8201854629045452 0.0 58-59 0.0 1.161787749205001E-4 0.0 0.9423015846907192 0.0 60-61 0.0 1.3452279201321061E-4 0.0 1.0714679237128584 0.0 62-63 0.0 1.3452279201321061E-4 0.0 1.2033186039028974 0.0 64-65 0.0 1.4063746437744745E-4 0.0 1.3504926530377142 0.0 66-67 0.0 1.4675213674168432E-4 0.0 1.5203337926267568 0.0 68-69 0.0 1.5286680910592116E-4 0.0 1.6944613175431296 0.0 70-71 0.0 1.956695156555791E-4 0.0 1.9044513958757512 0.0 72-73 0.0 1.956695156555791E-4 0.0 2.135274162953328 0.0 74-75 0.0 2.0178418801981593E-4 0.0 2.3497462961289353 0.0 76-77 0.0 2.1401353274828964E-4 0.0 2.5773221721811024 0.0 78-79 0.0 2.2012820511252648E-4 0.0 2.845364949939789 0.0 80-81 0.0 2.3235754984100016E-4 0.0 3.1720902383780563 0.0 82-83 0.0 2.3235754984100016E-4 0.0 3.507113137214593 0.0 84-85 0.0 2.3235754984100016E-4 0.0 3.849926128643168 0.0 86-87 0.0 2.4458689456947387E-4 0.0 4.258710320209493 0.0 88 0.0 2.4458689456947387E-4 0.0 4.598502663490135 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7535 0.0 42.315758 1 GTATCAA 11895 0.0 35.367527 1 ATCAACG 12465 0.0 32.348873 3 TCAACGC 12495 0.0 32.148346 4 CAACGCA 13275 0.0 30.259403 5 TATCAAC 14315 0.0 28.489983 2 AACGCAG 14730 0.0 27.58233 6 GTACATG 21025 0.0 23.246883 1 ACGCAGA 17465 0.0 22.822388 7 CGCAGAG 17635 0.0 22.011452 8 TACATGG 21460 0.0 21.865772 2 ACATGGG 21870 0.0 20.566734 3 GTGGTAT 4555 0.0 20.337029 1 GCAGAGT 20545 0.0 17.902813 9 GAGTACA 17020 0.0 17.470844 1 CATGGGG 15490 0.0 16.84783 4 AGAGTAC 17370 0.0 16.200462 10-11 TGGTATC 5440 0.0 15.896921 2 CAGAGTA 19285 0.0 15.541864 10-11 AGTACAT 17260 0.0 15.386924 2 >>END_MODULE