FastQCFastQC Report
Wed 25 May 2016
SRR2049522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049522_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8177053
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227870.27867007832773005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223550.2733870014050294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135570.16579322648391787No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142900.058.1815831
GTATCAA260100.040.4542581
ATCAACG324300.032.0788573
TCAACGC327200.031.7657784
TATCAAC336200.030.9434072
CAACGCA339450.030.6132225
AACGCAG358850.029.0360986
GTGGTAT59700.028.8624921
ACGCAGA407550.025.4157497
CGCAGAG409450.025.1026828
TGGTATC64300.025.099222
GCAGAGT439950.022.9671639
GTACATG211350.022.3421921
TACATGG214700.021.0824032
GAGTACT314950.020.91335712-13
CAGAGTA423850.020.77369510-11
AGAGTAC406200.020.43835610-11
ACATGGG218050.019.7011593
AGTACTT330850.018.94236212-13
GTACTTT347650.018.7907814-15
Produced by FastQC (version 0.11.5)