##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049522_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8177053 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75593811119972 31.0 31.0 34.0 30.0 34.0 2 31.803890105640747 31.0 31.0 34.0 30.0 34.0 3 31.833037281279697 33.0 31.0 34.0 30.0 34.0 4 35.544805934362905 37.0 35.0 37.0 33.0 37.0 5 35.680765062914475 37.0 35.0 37.0 33.0 37.0 6 35.76659170485993 37.0 35.0 37.0 33.0 37.0 7 35.78156494766513 37.0 35.0 37.0 33.0 37.0 8 35.78495541119765 37.0 35.0 37.0 33.0 37.0 9 37.51756714796883 39.0 37.0 39.0 35.0 39.0 10-11 37.48577439818477 39.0 37.0 39.0 34.5 39.0 12-13 37.474236378313805 39.0 37.0 39.0 34.5 39.0 14-15 38.756109750052985 40.0 38.0 41.0 35.0 41.0 16-17 38.76929555183267 40.0 38.0 41.0 35.0 41.0 18-19 38.8384483994417 40.0 38.0 41.0 35.0 41.0 20-21 38.86529609139136 40.0 38.0 41.0 35.0 41.0 22-23 38.84310612882172 40.0 38.0 41.0 35.0 41.0 24-25 38.8076993630835 40.0 38.0 41.0 35.0 41.0 26-27 38.720074273702274 40.0 38.0 41.0 34.5 41.0 28-29 38.63416269895768 40.0 38.0 41.0 34.0 41.0 30-31 38.52573378208506 40.0 38.0 41.0 34.0 41.0 32-33 38.39181927767864 40.0 38.0 41.0 34.0 41.0 34-35 38.29462044577674 40.0 38.0 41.0 34.0 41.0 36-37 38.165225968328684 40.0 38.0 41.0 34.0 41.0 38-39 38.080078666482905 40.0 38.0 41.0 33.5 41.0 40-41 37.96346312051543 40.0 37.5 41.0 33.0 41.0 42-43 37.82817709509771 40.0 37.0 41.0 33.0 41.0 44-45 37.65147700522425 40.0 37.0 41.0 33.0 41.0 46-47 37.414514312185574 40.0 37.0 41.0 32.5 41.0 48-49 37.19382618652466 40.0 37.0 41.0 32.0 41.0 50-51 36.985304730200475 40.0 37.0 41.0 31.0 41.0 52-53 36.885427549509586 40.0 36.0 41.0 31.0 41.0 54-55 36.75802015713974 40.0 36.0 41.0 31.0 41.0 56-57 36.58760815173878 39.0 35.5 41.0 31.0 41.0 58-59 36.416334650148414 39.0 35.0 41.0 31.0 41.0 60-61 36.22729784189977 39.0 35.0 41.0 30.5 41.0 62-63 36.026712924570745 39.0 35.0 41.0 30.0 41.0 64-65 35.74866446383557 38.0 35.0 40.0 30.0 41.0 66-67 35.498685345441686 37.5 35.0 40.0 29.5 41.0 68-69 35.19325006209449 37.0 35.0 40.0 29.0 41.0 70-71 34.90318700392427 36.5 34.0 39.5 29.0 41.0 72-73 34.58309399486588 36.0 34.0 39.0 29.0 41.0 74-75 34.24592203328021 36.0 34.0 39.0 29.0 40.5 76-77 33.95430976171978 35.0 34.0 38.5 29.0 40.0 78-79 33.630222037205826 35.0 34.0 37.0 28.0 39.0 80-81 33.32573452807509 35.0 34.0 37.0 28.0 39.0 82-83 33.031815802098876 35.0 34.0 36.0 28.0 39.0 84-85 32.759006148058475 35.0 33.5 36.0 27.0 37.0 86-87 32.48843030612618 35.0 33.0 36.0 27.0 37.0 88-89 32.28561805824177 35.0 33.0 35.0 26.5 37.0 90-91 32.14113691081616 35.0 33.0 35.0 27.0 36.0 92-93 31.98520499989422 35.0 33.0 35.0 26.0 36.0 94-95 31.807522587905446 35.0 33.0 35.0 26.0 36.0 96-97 31.6454678109583 35.0 33.0 35.0 26.0 36.0 98-99 31.470084332338313 35.0 33.0 35.0 25.0 35.0 100 31.36249538800837 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3367617707638608 1101 2 0.3833983512205421 1101 3 0.4535809042710852 1101 4 0.3602947209010168 1101 5 0.23484858356599858 1101 6 0.22003803590282445 1101 7 0.2819879301055863 1101 8 0.2833483843311555 1101 9 0.32706470986547487 1101 10-11 0.3587391287851318 1101 12-13 0.31220488791983314 1101 14-15 0.46132299942433264 1101 16-17 0.4883352669324523 1101 18-19 0.4394036271693551 1101 20-21 0.4112944044899649 1101 22-23 0.4535212402641875 1101 24-25 0.48049183862214306 1101 26-27 0.4589384260553544 1101 28-29 0.46770575332792674 1101 30-31 0.47109076956167684 1101 32-33 0.4814219064785519 1101 34-35 0.48250703373852133 1101 36-37 0.4886108097819175 1101 38-39 0.47496772424432265 1101 40-41 0.51001306531019 1101 42-43 0.43205467520289176 1101 44-45 0.5170941066204549 1101 46-47 0.5411122308730185 1101 48-49 0.5297289865890278 1101 50-51 0.5498393192046933 1101 52-53 0.5662307896144156 1101 54-55 0.49199954202929064 1101 56-57 0.45150853577156624 1101 58-59 0.45909368752714386 1101 60-61 0.513006968281239 1101 62-63 0.49203167361378775 1101 64-65 0.47664835687114504 1101 66-67 0.501860216230618 1101 68-69 0.49807212171230475 1101 70-71 0.4876774335582894 1101 72-73 0.42669350203223644 1101 74-75 0.41386498638131997 1101 76-77 0.3654491163932505 1101 78-79 0.3758606942379643 1101 80-81 0.3898905116054294 1101 82-83 0.4197276355010331 1101 84-85 0.43345735626526505 1101 86-87 0.5236923987844762 1101 88-89 0.524308783211545 1101 90-91 0.5413570541483921 1101 92-93 0.5275327576780313 1101 94-95 0.5195165142120963 1101 96-97 0.5613247586205361 1101 98-99 0.5609915199107824 1101 100 0.622102143583799 1102 1 0.18519034881456875 1102 2 0.27231874934910394 1102 3 0.32572906463139617 1102 4 0.3233733498081435 1102 5 0.23038620402503796 1102 6 0.19755654297826908 1102 7 0.22553605087891526 1102 8 0.2665425864259632 1102 9 0.33550340629746955 1102 10-11 0.26667115804146135 1102 12-13 0.2737368489745009 1102 14-15 0.3722123875668686 1102 16-17 0.38468330410375273 1102 18-19 0.3656788348815496 1102 20-21 0.35662795950664616 1102 22-23 0.3140250002929861 1102 24-25 0.33155979326161145 1102 26-27 0.300581086153926 1102 28-29 0.2849870168036901 1102 30-31 0.380224379901307 1102 32-33 0.405540619030603 1102 34-35 0.41448010014384806 1102 36-37 0.46970123409833775 1102 38-39 0.4318288600186406 1102 40-41 0.4705118985244354 1102 42-43 0.4664172722997648 1102 44-45 0.4617798694245394 1102 46-47 0.5477371067791736 1102 48-49 0.5929706175473655 1102 50-51 0.5814020336967971 1102 52-53 0.5748281043047214 1102 54-55 0.5512110200435956 1102 56-57 0.5645430272722081 1102 58-59 0.5301514860301708 1102 60-61 0.5645412270400172 1102 62-63 0.5340635194336656 1102 64-65 0.5273327168148896 1102 66-67 0.5819671677258995 1102 68-69 0.562982320728814 1102 70-71 0.5735103270557289 1102 72-73 0.6223856436162052 1102 74-75 0.5761311966207998 1102 76-77 0.5365344528530755 1102 78-79 0.506983924753527 1102 80-81 0.4723313492207666 1102 82-83 0.5349445846566496 1102 84-85 0.5328654999447764 1102 86-87 0.5531742099943102 1102 88-89 0.549028294462218 1102 90-91 0.520712477649468 1102 92-93 0.5828822330177346 1102 94-95 0.5982289371423484 1102 96-97 0.5730581515790405 1102 98-99 0.6276204290928327 1102 100 0.695080921994343 1103 1 0.2766616197825904 1103 2 0.29405201770507716 1103 3 0.3880842955910069 1103 4 0.33341022470848003 1103 5 0.26712078904756 1103 6 0.2549838413127432 1103 7 0.25486435592401335 1103 8 0.23983352285137727 1103 9 0.32208320257957723 1103 10-11 0.2510358623662796 1103 12-13 0.2477509224024459 1103 14-15 0.35104650242956836 1103 16-17 0.3666080735902639 1103 18-19 0.34294344625720896 1103 20-21 0.37788638641190886 1103 22-23 0.351251278851457 1103 24-25 0.3407144492556782 1103 26-27 0.3514750514311089 1103 28-29 0.36311784762239796 1103 30-31 0.38895165632806794 1103 32-33 0.38453706600105875 1103 34-35 0.39953376980562183 1103 36-37 0.4532679576814189 1103 38-39 0.3909324666609635 1103 40-41 0.40745916487689726 1103 42-43 0.43658873636775297 1103 44-45 0.5032788417033558 1103 46-47 0.4576196459641082 1103 48-49 0.45915783682351474 1103 50-51 0.5093486948211918 1103 52-53 0.48453585597675186 1103 54-55 0.48727167080968314 1103 56-57 0.49546536148771025 1103 58-59 0.46213337562621604 1103 60-61 0.4546356002756724 1103 62-63 0.4662783106357793 1103 64-65 0.4479719286519952 1103 66-67 0.4160436513043422 1103 68-69 0.4414489888959068 1103 70-71 0.43054206665597405 1103 72-73 0.4484873731766541 1103 74-75 0.4881408051143623 1103 76-77 0.4583923687594833 1103 78-79 0.4123184712589065 1103 80-81 0.3815530284288826 1103 82-83 0.41497585874308385 1103 84-85 0.45876703697294374 1103 86-87 0.5033303054948277 1103 88-89 0.49358644781383987 1103 90-91 0.4594181822940371 1103 92-93 0.5214852668338565 1103 94-95 0.4778747392578673 1103 96-97 0.5192533169361795 1103 98-99 0.5609494280787466 1103 100 0.5303126527477993 1104 1 0.1844256975644818 1104 2 0.1686673277365216 1104 3 0.3015501035592294 1104 4 0.1948816412193466 1104 5 0.1857459409381761 1104 6 0.18689336726654915 1104 7 0.15461099937134293 1104 8 0.19300580657850475 1104 9 0.20509814725143372 1104 10-11 0.23469953262323173 1104 12-13 0.23491927496274911 1104 14-15 0.31869593113778905 1104 16-17 0.30332950308975626 1104 18-19 0.27676772412577577 1104 20-21 0.30170590701177247 1104 22-23 0.26647913235876075 1104 24-25 0.2659646148023498 1104 26-27 0.264538190887734 1104 28-29 0.2648405840359018 1104 30-31 0.27828754738195016 1104 32-33 0.2817189648691354 1104 34-35 0.23570105971432298 1104 36-37 0.271175245523807 1104 38-39 0.3111597429902986 1104 40-41 0.2526858712323943 1104 42-43 0.25329200213566594 1104 44-45 0.3489916255683383 1104 46-47 0.3786795396633593 1104 48-49 0.36909139609532815 1104 50-51 0.35455685054093067 1104 52-53 0.3747894261107021 1104 54-55 0.3272019428791708 1104 56-57 0.3139344125859225 1104 58-59 0.34853593548258033 1104 60-61 0.33785666783118273 1104 62-63 0.3166862911341113 1104 64-65 0.31512603737520095 1104 66-67 0.3520036889760121 1104 68-69 0.25372407700454147 1104 70-71 0.2880985574206605 1104 72-73 0.3486356574875771 1104 74-75 0.38922308374181824 1104 76-77 0.34815417223978784 1104 78-79 0.3248644283136386 1104 80-81 0.34279331312933436 1104 82-83 0.29794657217320974 1104 84-85 0.30500442985069753 1104 86-87 0.37399360969720163 1104 88-89 0.34666769040258316 1104 90-91 0.34203498222439777 1104 92-93 0.3651297130807407 1104 94-95 0.2956013517477736 1104 96-97 0.3029468122929515 1104 98-99 0.3448087773491437 1104 100 0.41025215415587013 1105 1 0.24607337213116764 1105 2 0.23427538007077686 1105 3 0.3351034110298414 1105 4 0.26535286724477203 1105 5 0.18785047534024102 1105 6 0.19286423352613724 1105 7 0.19176298367282385 1105 8 0.2332357625779764 1105 9 0.23710213135964864 1105 10-11 0.23093021043575845 1105 12-13 0.21297112483378555 1105 14-15 0.33964936804424894 1105 16-17 0.2743772649682015 1105 18-19 0.2750738996401694 1105 20-21 0.27357501742994117 1105 22-23 0.29084759482363154 1105 24-25 0.28372245642244565 1105 26-27 0.2741419139732244 1105 28-29 0.2981693383091155 1105 30-31 0.28693608422965866 1105 32-33 0.2619048034168543 1105 34-35 0.3094166557568059 1105 36-37 0.3455872029489768 1105 38-39 0.3310223634462659 1105 40-41 0.34236483878633095 1105 42-43 0.3437930006070573 1105 44-45 0.3720306125172783 1105 46-47 0.41680991104549747 1105 48-49 0.43360120730198304 1105 50-51 0.4762292406253579 1105 52-53 0.4128159742302273 1105 54-55 0.39617883296568124 1105 56-57 0.46728183997299055 1105 58-59 0.4334515092652964 1105 60-61 0.4411388558332092 1105 62-63 0.4113887198529369 1105 64-65 0.4993927636348374 1105 66-67 0.4714739442732778 1105 68-69 0.490700154511579 1105 70-71 0.474956098786393 1105 72-73 0.4560722120430398 1105 74-75 0.45645492008913635 1105 76-77 0.41188487103288196 1105 78-79 0.3762249734043053 1105 80-81 0.39797810672249057 1105 82-83 0.4183818725387738 1105 84-85 0.4197246130196248 1105 86-87 0.47298900912690556 1105 88-89 0.4374121042140473 1105 90-91 0.4065719153997023 1105 92-93 0.4283448704052759 1105 94-95 0.43490985541277283 1105 96-97 0.4255907554959819 1105 98-99 0.48458809109469314 1105 100 0.500851934442462 1106 1 0.20169711924118872 1106 2 0.20106598634663442 1106 3 0.30511995514670787 1106 4 0.24461861496513393 1106 5 0.16127775127174715 1106 6 0.22322443707779627 1106 7 0.1938407857376916 1106 8 0.20672564034239116 1106 9 0.237555924422864 1106 10-11 0.24130456488905594 1106 12-13 0.23282639720908094 1106 14-15 0.2987270600675913 1106 16-17 0.32249172852951347 1106 18-19 0.30410888516737344 1106 20-21 0.2561980374951034 1106 22-23 0.23061191587481034 1106 24-25 0.28592809478785597 1106 26-27 0.2802392299367895 1106 28-29 0.32560669017645694 1106 30-31 0.31048273838771223 1106 32-33 0.3675283463833665 1106 34-35 0.3795757606374863 1106 36-37 0.3551951536626561 1106 38-39 0.39363134627687657 1106 40-41 0.44102994272624585 1106 42-43 0.41990890212260723 1106 44-45 0.42139176580987936 1106 46-47 0.36292965078570916 1106 48-49 0.33640594644952415 1106 50-51 0.39838174811032445 1106 52-53 0.4172756476718149 1106 54-55 0.3181982813424753 1106 56-57 0.4123251554700573 1106 58-59 0.40367917696943323 1106 60-61 0.3968042547523325 1106 62-63 0.422714660708742 1106 64-65 0.39438250044224077 1106 66-67 0.3880352418577857 1106 68-69 0.37645677749055295 1106 70-71 0.4153540845660544 1106 72-73 0.40700779196103554 1106 74-75 0.4081691523574875 1106 76-77 0.3390909887272642 1106 78-79 0.38408652046525305 1106 80-81 0.3318560492185014 1106 82-83 0.36971940286674254 1106 84-85 0.37022415872681336 1106 86-87 0.42054349886823417 1106 88-89 0.4035790242962989 1106 90-91 0.3629157534355656 1106 92-93 0.37558155932554627 1106 94-95 0.39049357406290497 1106 96-97 0.42478081529435485 1106 98-99 0.4646340328572194 1106 100 0.5060857625445436 1107 1 0.12886795696736897 1107 2 0.17460911123735912 1107 3 0.2180601027463993 1107 4 0.2038326639498962 1107 5 0.16056508504865974 1107 6 0.14764215478699327 1107 7 0.13227812664477057 1107 8 0.18505946595499978 1107 9 0.1733912277492351 1107 10-11 0.18858586938358712 1107 12-13 0.17516570831942602 1107 14-15 0.20566416849972313 1107 16-17 0.21178183378618343 1107 18-19 0.22685285334475935 1107 20-21 0.2442360365497791 1107 22-23 0.25822054929702887 1107 24-25 0.28252362447160095 1107 26-27 0.240036445913546 1107 28-29 0.2789690627313064 1107 30-31 0.26041019289042566 1107 32-33 0.29255491369721653 1107 34-35 0.26233169067565854 1107 36-37 0.3060403148376949 1107 38-39 0.28110744620571637 1107 40-41 0.30174497048834326 1107 42-43 0.3288189467570959 1107 44-45 0.3289740228735738 1107 46-47 0.3309424998003365 1107 48-49 0.3449394762741136 1107 50-51 0.3790904971218225 1107 52-53 0.3498919701108818 1107 54-55 0.3368896523700329 1107 56-57 0.40538976388380377 1107 58-59 0.41154772684082985 1107 60-61 0.39126104997571787 1107 62-63 0.37229636347647244 1107 64-65 0.36127487978389183 1107 66-67 0.3302868887159036 1107 68-69 0.3670755112221187 1107 70-71 0.37098297396649116 1107 72-73 0.3632536947434062 1107 74-75 0.33568574181865074 1107 76-77 0.2755015405320407 1107 78-79 0.27630111498654486 1107 80-81 0.2981643856263858 1107 82-83 0.3567404685655262 1107 84-85 0.38405289669444187 1107 86-87 0.41351063248193753 1107 88-89 0.3741591184845632 1107 90-91 0.39407251162413104 1107 92-93 0.37516331464977526 1107 94-95 0.40232173110595326 1107 96-97 0.41319778179588695 1107 98-99 0.4581171662480834 1107 100 0.44698204718497436 1108 1 0.12809559422516514 1108 2 0.1425004210839056 1108 3 0.21066251751312848 1108 4 0.15339063443863665 1108 5 0.14461096055596556 1108 6 0.17183603665401392 1108 7 0.12664947679791538 1108 8 0.15585173518267226 1108 9 0.119790267042724 1108 10-11 0.1546800219657385 1108 12-13 0.10515082512948482 1108 14-15 0.16382927142964832 1108 16-17 0.22023566814132067 1108 18-19 0.21381459104925682 1108 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-0.6142820294617124 2215 30-31 -0.6009438363137178 2215 32-33 -0.6597416082878595 2215 34-35 -0.7618351342504397 2215 36-37 -0.7954500934229571 2215 38-39 -0.7676590077034575 2215 40-41 -0.7182905729583382 2215 42-43 -0.7108524843302533 2215 44-45 -0.8068411329429921 2215 46-47 -0.834734030893074 2215 48-49 -0.8245421033417486 2215 50-51 -0.8142919098256911 2215 52-53 -0.9158207573566202 2215 54-55 -0.8052449752820294 2215 56-57 -0.7575945049251303 2215 58-59 -0.7594748533317812 2215 60-61 -0.9174639044536761 2215 62-63 -0.9161356983843021 2215 64-65 -0.7817755729192655 2215 66-67 -0.6719592032904487 2215 68-69 -0.6100792823345316 2215 70-71 -0.7011183268855063 2215 72-73 -0.6333810856898765 2215 74-75 -0.6783886200305957 2215 76-77 -0.7004232424090517 2215 78-79 -0.6634547921484355 2215 80-81 -0.6901833827937907 2215 82-83 -0.701566315732542 2215 84-85 -0.6887879880488583 2215 86-87 -0.6506843413200372 2215 88-89 -0.6479817573718627 2215 90-91 -0.6728266442443669 2215 92-93 -0.8377227420038551 2215 94-95 -0.8339640233533139 2215 96-97 -0.8429440032407243 2215 98-99 -0.8668478695753592 2215 100 -0.9250484579304583 2216 1 -0.6110215736109446 2216 2 -0.9879406274151243 2216 3 -0.6690448354823069 2216 4 -1.1628447747594919 2216 5 -0.5785261946931826 2216 6 -0.8160325094457335 2216 7 -0.5209076567298396 2216 8 -0.4761341149885965 2216 9 -0.5643160954637807 2216 10-11 -0.8107562252111649 2216 12-13 -0.6457615329025259 2216 14-15 -0.8876563735154832 2216 16-17 -0.8098910142569764 2216 18-19 -0.8359722866724937 2216 20-21 -0.683455371838825 2216 22-23 -0.7241499796295656 2216 24-25 -0.7899604471906727 2216 26-27 -0.9163666647744009 2216 28-29 -1.1557238838586628 2216 30-31 -1.2339308167781624 2216 32-33 -0.9614860501805396 2216 34-35 -0.9886352835540535 2216 36-37 -0.9435453090025732 2216 38-39 -1.0564556513241214 2216 40-41 -0.9288971088942759 2216 42-43 -1.527048611942817 2216 44-45 -1.2581904318564412 2216 46-47 -1.0328082443972448 2216 48-49 -1.0357715403210008 2216 50-51 -1.2821349366362895 2216 52-53 -1.1330857892887778 2216 54-55 -1.2552225096909098 2216 56-57 -1.4194596205653909 2216 58-59 -1.211731062891218 2216 60-61 -1.4316808335024334 2216 62-63 -1.4302920541452053 2216 64-65 -1.4551502074156062 2216 66-67 -1.2967670386782046 2216 68-69 -1.6206656918026994 2216 70-71 -1.3710093249881936 2216 72-73 -1.233094505553268 2216 74-75 -1.267698069538561 2216 76-77 -1.384490610162274 2216 78-79 -1.4576111866862433 2216 80-81 -1.2490881064219934 2216 82-83 -1.2590647821682452 2216 84-85 -1.3044803410418382 2216 86-87 -1.4709902898983493 2216 88-89 -1.278662481044094 2216 90-91 -1.1708154359658245 2216 92-93 -1.1721679407217636 2216 94-95 -1.3030904095653462 2216 96-97 -1.4185093205709478 2216 98-99 -1.4569751991921152 2216 100 -1.4262584226459687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 494.0 3 37.0 4 66.0 5 148.0 6 460.0 7 1148.0 8 2610.0 9 4416.0 10 6285.0 11 8827.0 12 12517.0 13 18023.0 14 21220.0 15 27347.0 16 35672.0 17 50914.0 18 50335.0 19 27091.0 20 20373.0 21 19707.0 22 20889.0 23 22995.0 24 27414.0 25 33789.0 26 42758.0 27 55904.0 28 74162.0 29 97801.0 30 127753.0 31 166175.0 32 213494.0 33 279207.0 34 381657.0 35 556507.0 36 911887.0 37 1667385.0 38 2290749.0 39 895824.0 40 3013.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.25660339125408 20.730341313282548 14.887762538795613 23.125292756667754 2 15.52507154791258 20.946374469581773 39.468863908911764 24.059690073593888 3 18.142318510103824 25.23314939991217 30.850588836834003 25.773943253150005 4 12.874044858018419 17.56879673016343 37.85405404809769 31.70310436372047 5 12.74121443260011 37.52466244868933 35.42400211326902 14.310121005441545 6 30.383583965232923 38.140331850625635 17.332943456054178 14.143140728087259 7 26.31921304655846 33.04244206317362 22.383675390143612 18.25466950012431 8 24.859995404212253 36.117180602840655 20.33244739883672 18.690376594110372 9 24.541096559674905 16.83106383025559 21.892573199102316 36.735266410967185 10-11 23.64744975971172 27.098167273710956 28.685756347671955 20.56862661890537 12-13 24.45665164271009 24.590189996968956 29.035239658206873 21.91791870211408 14-15 21.72026107950062 26.633316821672658 26.88273441416736 24.763687684659363 16-17 20.660542373884578 29.82592873006938 27.66560275443977 21.847926141606273 18-19 20.519030511359045 28.137484250132662 30.59909236249294 20.744392876015358 20-21 21.505498374536643 27.398483292208088 30.468843726462335 20.627174606792938 22-23 21.25096902270292 27.218179948203836 30.57952541092738 20.95132561816586 24-25 21.141198424420143 27.266785478827156 30.521943541273366 21.07007255547934 26-27 20.905019204351493 27.282536874837426 30.57438297146906 21.23806094934202 28-29 20.949850171889892 27.27135146875329 30.73916016278268 21.03963819657414 30-31 21.261610195344236 27.60552995639674 30.287103753424304 20.845756094834716 32-33 20.91426458896622 27.4144670457682 30.630277191550547 21.040991173715028 34-35 21.096200550491723 27.182231789374484 30.528639107512205 21.192928552621588 36-37 21.410402989928034 27.39913756215106 30.080519228626745 21.109940219294163 38-39 21.22355694649405 27.512552505162923 30.25338101636372 21.010509531979306 40-41 21.16418347783731 27.347199535089228 30.255845229326507 21.232771757746953 42-43 21.14143689664235 27.40467134064069 30.086994666660473 21.36689709605649 44-45 21.35115181471858 27.570482911141703 29.854728836904933 21.223636437234784 46-47 21.4373381216925 27.747551593465275 29.435623078387774 21.379487206454453 48-49 21.46830282254499 27.68416078506523 29.21846660404427 21.629069788345507 50-51 21.524258189350125 27.74182214545998 29.020748673146667 21.71317099204322 52-53 21.521867352455708 27.830601073516338 28.97556124437496 21.671970329652993 54-55 21.604678360284566 28.133399648993347 28.780717209488554 21.481204781233533 56-57 21.4127510241159 27.881414000863145 28.859199029283534 21.846635945737418 58-59 21.31622160457158 27.526272487883 29.20551042751704 21.951995480028387 60-61 21.528098203594865 27.78154305713807 28.933162106201344 21.757196633065725 62-63 21.341784136656567 27.798731401153937 29.045763797788766 21.813720664400734 64-65 21.3377606822409 27.628517266550674 29.13375393311013 21.899968118098293 66-67 21.26272753765935 27.639572594185214 29.025505888246045 22.07219397990939 68-69 21.566687900885565 27.81743618391614 28.77821630849158 21.83765960670672 70-71 21.53660371285352 27.56926609134122 28.836984424584262 22.057145771221002 72-73 21.372669346768326 27.681237971675127 28.941637042098172 22.004455639458374 74-75 21.417630532662564 27.74927593107199 28.738605460916055 22.094488075349396 76-77 21.414132940070218 27.713278854863727 28.636478203088572 22.236110001977487 78-79 21.497068928934596 27.75895204196626 28.721359610959706 22.02261941813944 80-81 21.386122788307656 27.667123903077457 28.72184130058556 22.224912008029328 82-83 21.25977241743872 27.64389159514775 28.866902510171016 22.22943347724251 84-85 21.30169022996427 27.558033438208117 28.684551757216198 22.455724574611416 86-87 21.349981714068942 27.631781455699528 28.651085296454355 22.36715153377718 88-89 21.223216570528667 27.514870128857634 28.70023685199334 22.56167644862036 90-91 21.115185998957937 27.44408939240118 28.658353928070746 22.782370680570136 92-93 21.149720719513752 27.543555275718965 28.547273695732585 22.759450309034694 94-95 21.00337660531837 27.32156214513649 28.747317399248956 22.927743850296185 96-97 20.980929312911304 27.343332291393036 28.706623120410633 22.96911527528503 98-99 20.966560126608012 27.31847838269782 28.61650158776708 23.098459902927086 100 21.004924438419938 27.242489366174986 28.544951781056387 23.207634414348686 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1090.0 1 870.0 2 1403.5 3 2246.5 4 1555.5 5 882.5 6 1906.0 7 4842.0 8 7889.0 9 8992.5 10 7337.0 11 5186.0 12 5022.0 13 5567.5 14 6261.5 15 7064.5 16 7892.0 17 8858.0 18 10023.0 19 11351.5 20 13235.5 21 16045.5 22 20736.0 23 27995.0 24 36709.0 25 46566.0 26 60652.5 27 79542.0 28 103174.5 29 127164.5 30 146561.5 31 165785.5 32 185389.5 33 203094.0 34 220688.5 35 237223.5 36 250671.5 37 263137.5 38 274150.5 39 281372.5 40 286009.5 41 286724.0 42 284039.0 43 282522.5 44 280746.0 45 276562.5 46 271114.0 47 265059.0 48 257935.0 49 253189.5 50 249172.0 51 239281.5 52 225344.5 53 215452.5 54 208950.0 55 199411.0 56 184681.5 57 165682.0 58 149085.5 59 135423.5 60 118639.5 61 99996.0 62 83231.0 63 68016.0 64 54244.5 65 42927.0 66 33652.0 67 25741.0 68 19513.0 69 14519.0 70 10526.5 71 7673.0 72 5606.0 73 3896.5 74 2653.0 75 1695.0 76 966.5 77 597.0 78 401.5 79 269.0 80 148.5 81 60.5 82 36.5 83 20.0 84 13.0 85 10.0 86 6.0 87 4.5 88 3.5 89 2.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05793040597877988 2 1.8344017092710542E-4 3 0.0 4 0.11679024215692378 5 0.0018099430198141068 6 0.0014797507121453168 7 0.0 8 0.0 9 7.337606837084216E-5 10-11 0.0 12-13 1.834401709271054E-5 14-15 4.280270654965793E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.956695156555791E-4 30-31 3.2407763530455287E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.2840811964897377E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 9.783475782778955E-5 80-81 3.4853632476150023E-4 82-83 2.5070156693371074E-4 84-85 0.0 86-87 1.834401709271054E-5 88-89 6.726139600660532E-5 90-91 5.503205127813163E-5 92-93 7.154166666157111E-4 94-95 6.542699429733426E-4 96-97 1.2229344728473693E-5 98-99 4.280270654965793E-5 100 9.783475782778955E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8177053.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.70904696087267 #Duplication Level Percentage of deduplicated Percentage of total 1 73.95771571873253 41.94071573812474 2 15.365413042921135 17.4271585964844 3 4.791805954644074 8.152162467279 4 2.0202192992615924 4.582588444523477 5 1.0575920740288396 2.998751929577409 6 0.6476727987020784 2.20373442941256 7 0.4076613224275107 1.618265955437121 8 0.29313216973646183 1.329859678346201 9 0.22726851735476333 1.159936292105926 >10 1.1346955752320729 11.434547869724687 >50 0.06561338194570279 2.5329285467139897 >100 0.029506392816883664 2.935664406709846 >500 0.0011216423626644596 0.41639759432602513 >1k 4.95871355432922E-4 0.4667251148210615 >5k 2.1559619549690997E-5 0.08289400737463391 >10k+ 6.467885864907299E-5 0.7176689290388844 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22787 0.27867007832773005 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22355 0.2733870014050294 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13557 0.16579322648391787 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.8917378913894774E-5 0.0 0.0 0.0 6 0.0 4.8917378913894774E-5 0.0 0.0 0.0 7 0.0 4.8917378913894774E-5 0.0 0.0 0.0 8 0.0 4.8917378913894774E-5 0.0 0.0 0.0 9 0.0 4.8917378913894774E-5 0.0 1.2229344728473693E-5 0.0 10-11 0.0 4.8917378913894774E-5 0.0 1.2229344728473693E-5 0.0 12-13 0.0 4.8917378913894774E-5 0.0 2.4458689456947387E-5 0.0 14-15 0.0 5.503205127813162E-5 0.0 3.668803418542108E-5 0.0 16-17 0.0 6.114672364236847E-5 0.0 7.337606837084216E-5 0.0 18-19 0.0 8.560541309931585E-5 0.0 8.560541309931585E-5 0.0 20-21 0.0 8.560541309931585E-5 0.0 1.2840811964897377E-4 0.0 22-23 0.0 8.560541309931585E-5 0.0 2.9350427348336864E-4 0.0 24-25 0.0 9.783475782778955E-5 0.0 5.503205127813162E-4 0.0 26-27 0.0 1.1006410255626324E-4 0.0 0.0010578383190129744 0.0 28-29 0.0 1.1006410255626324E-4 0.0 0.0029166987177409757 0.0 30-31 0.0 1.1006410255626324E-4 0.0 0.008126399572070769 0.0 32-33 0.0 1.1006410255626324E-4 0.0 0.0201967628190743 0.0 34-35 0.0 1.161787749205001E-4 0.0 0.0408398967207379 0.0 36-37 0.0 1.4063746437744745E-4 0.0 0.07239772079256426 0.0 38-39 0.0 1.5286680910592116E-4 0.0 0.11499252848183814 0.0 40-41 0.0 1.7121082619863168E-4 0.0 0.1627114316123425 0.0 42-43 0.0 1.956695156555791E-4 0.0 0.2132430840303958 0.0 44-45 0.0 1.956695156555791E-4 0.0 0.27628535610567767 0.0 46-47 0.0 1.956695156555791E-4 0.0 0.3539600391485783 0.0 48-49 0.0 1.956695156555791E-4 0.0 0.4378166559517225 0.0 50-51 0.0 2.0789886038405277E-4 0.0 0.5256050070850709 0.0 52-53 0.0 2.2012820511252648E-4 0.0 0.6144206231756111 0.0 54-55 0.0 2.2012820511252648E-4 0.0 0.7182599892650812 0.0 56-57 0.0 2.2012820511252648E-4 0.0 0.8440938318487112 0.0 58-59 0.0 2.323575498410002E-4 0.0 0.984505053348682 0.0 60-61 0.0 2.4458689456947387E-4 0.0 1.1332933759876571 0.0 62-63 0.0 2.4458689456947387E-4 0.0 1.284466420848685 0.0 64-65 0.0 2.4458689456947387E-4 0.0 1.452907300466317 0.0 66-67 0.0 2.507015669337107E-4 0.0 1.6545447363493913 0.0 68-69 0.0 2.5681623929794755E-4 0.0 1.8830561572732867 0.0 70-71 0.0 2.5681623929794755E-4 0.0 2.1297098111018724 0.0 72-73 0.0 2.5681623929794755E-4 0.0 2.3921026315960043 0.0 74-75 6.114672364236847E-6 2.751602563906581E-4 0.0 2.66483536305806 0.0 76-77 1.2229344728473693E-5 2.8127492875489496E-4 0.0 2.9652247576235595 0.0 78-79 1.2229344728473693E-5 2.8127492875489496E-4 0.0 3.3070532868014917 0.0 80-81 1.2229344728473693E-5 2.9350427348336864E-4 0.0 3.6817237212477405 1.2229344728473693E-5 82-83 1.2229344728473693E-5 2.9350427348336864E-4 0.0 4.066055338029484 1.2229344728473693E-5 84-85 1.2229344728473693E-5 2.996189458476055E-4 0.0 4.458543927745118 1.2229344728473693E-5 86-87 1.2229344728473693E-5 3.057336182118423E-4 0.0 4.8834586250083 1.2229344728473693E-5 88 1.2229344728473693E-5 3.057336182118423E-4 0.0 5.227127670567868 2.4458689456947387E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14290 0.0 58.181583 1 GTATCAA 26010 0.0 40.454258 1 ATCAACG 32430 0.0 32.078857 3 TCAACGC 32720 0.0 31.765778 4 TATCAAC 33620 0.0 30.943407 2 CAACGCA 33945 0.0 30.613222 5 AACGCAG 35885 0.0 29.036098 6 GTGGTAT 5970 0.0 28.862492 1 ACGCAGA 40755 0.0 25.415749 7 CGCAGAG 40945 0.0 25.102682 8 TGGTATC 6430 0.0 25.09922 2 GCAGAGT 43995 0.0 22.967163 9 GTACATG 21135 0.0 22.342192 1 TACATGG 21470 0.0 21.082403 2 GAGTACT 31495 0.0 20.913357 12-13 CAGAGTA 42385 0.0 20.773695 10-11 AGAGTAC 40620 0.0 20.438356 10-11 ACATGGG 21805 0.0 19.701159 3 AGTACTT 33085 0.0 18.942362 12-13 GTACTTT 34765 0.0 18.79078 14-15 >>END_MODULE