FastQCFastQC Report
Wed 25 May 2016
SRR2049521_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049521_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7714381
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78700.045.0670051
GTATCAA123900.036.5571371
TCAACGC132350.033.1734284
ATCAACG132450.033.1483763
CAACGCA142300.030.745975
TATCAAC148500.029.7035122
AACGCAG154000.028.4096576
GTACATG226650.024.6585251
ACGCAGA180950.023.8951177
TACATGG231200.023.5715832
CGCAGAG186750.023.0381628
ACATGGG234250.022.3690383
GTGGTAT47000.020.3631041
GCAGAGT216350.018.5478959
CATGGGT67400.018.5249424
CATGGGG141850.018.470034
CGCCGTA17000.017.30066194
TACACCG9200.017.2425235
GAGTACA175500.017.2349721
TGGTATC52100.017.0898592
Produced by FastQC (version 0.11.5)