FastQCFastQC Report
Wed 25 May 2016
SRR2049521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049521_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7714381
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262830.34070134726298845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252840.3277515072174942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150360.19490870362767926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150300.056.3922921
GTATCAA267200.041.7302971
GTGGTAT58800.033.4738121
ATCAACG340800.032.283073
TCAACGC343350.032.057024
TATCAAC346650.031.9285262
CAACGCA355800.030.9884555
AACGCAG372850.029.6090136
TGGTATC66450.028.7517192
ACGCAGA427850.025.7035877
CGCAGAG427000.025.5346268
GTACATG235450.023.558781
GCAGAGT462100.023.1882679
TACATGG237300.022.4680272
ATAACGC6300.021.6614423
GAGTACT322850.021.26761612-13
ACATGGG244050.021.0944613
CAGAGTA447050.020.61010410-11
AGAGTAC424450.020.40648710-11
AGTACTT335400.019.4559412-13
Produced by FastQC (version 0.11.5)