##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049521_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7714381 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76458590779999 31.0 31.0 34.0 30.0 34.0 2 31.945329897499228 33.0 31.0 34.0 30.0 34.0 3 31.966894816317733 33.0 31.0 34.0 30.0 34.0 4 35.58847352755846 37.0 35.0 37.0 33.0 37.0 5 35.7210770378077 37.0 35.0 37.0 33.0 37.0 6 35.79063803045248 37.0 35.0 37.0 35.0 37.0 7 35.77015070943476 37.0 35.0 37.0 33.0 37.0 8 35.76735061439148 37.0 35.0 37.0 33.0 37.0 9 37.51029628948842 39.0 37.0 39.0 35.0 39.0 10-11 37.467101119325065 39.0 37.0 39.0 34.5 39.0 12-13 37.44738540914689 39.0 37.0 39.0 34.0 39.0 14-15 38.703394154372205 40.0 38.0 41.0 34.0 41.0 16-17 38.71281752871682 40.0 38.0 41.0 34.0 41.0 18-19 38.79155560245209 40.0 38.0 41.0 35.0 41.0 20-21 38.822617005823275 40.0 38.0 41.0 35.0 41.0 22-23 38.79397925770065 40.0 38.0 41.0 35.0 41.0 24-25 38.7557975163529 40.0 38.0 41.0 34.0 41.0 26-27 38.66387361474628 40.0 38.0 41.0 34.0 41.0 28-29 38.575812109876345 40.0 38.0 41.0 34.0 41.0 30-31 38.456550499644756 40.0 38.0 41.0 34.0 41.0 32-33 38.30838540383214 40.0 38.0 41.0 34.0 41.0 34-35 38.20450655729863 40.0 38.0 41.0 34.0 41.0 36-37 38.070032773854436 40.0 38.0 41.0 33.5 41.0 38-39 37.98212617447855 40.0 37.5 41.0 33.0 41.0 40-41 37.861077447432265 40.0 37.0 41.0 33.0 41.0 42-43 37.71718113222565 40.0 37.0 41.0 33.0 41.0 44-45 37.528570600803874 40.0 37.0 41.0 33.0 41.0 46-47 37.27290673094834 40.0 37.0 41.0 32.0 41.0 48-49 37.037205123781156 40.0 37.0 41.0 31.0 41.0 50-51 36.822638005563896 40.0 36.5 41.0 31.0 41.0 52-53 36.722247643718916 40.0 36.0 41.0 31.0 41.0 54-55 36.597870652227314 39.5 36.0 41.0 31.0 41.0 56-57 36.429128467987255 39.0 35.5 41.0 30.5 41.0 58-59 36.25826297664064 39.0 35.0 41.0 30.0 41.0 60-61 36.07406057595548 39.0 35.0 41.0 30.0 41.0 62-63 35.8787964322737 39.0 35.0 41.0 30.0 41.0 64-65 35.60284895184721 38.0 35.0 40.0 29.0 41.0 66-67 35.34973881896681 37.5 35.0 40.0 29.0 41.0 68-69 35.042231515399614 37.0 34.5 40.0 29.0 41.0 70-71 34.74703433755735 37.0 34.0 39.5 28.0 41.0 72-73 34.42328360499695 36.0 34.0 39.0 28.0 41.0 74-75 34.085174883117645 36.0 34.0 39.0 28.0 40.5 76-77 33.78560645371287 35.0 34.0 38.5 28.0 40.0 78-79 33.451908519944766 35.0 34.0 37.0 28.0 39.0 80-81 33.13682918953575 35.0 34.0 37.0 27.0 39.0 82-83 32.83277226001671 35.0 33.5 36.0 27.0 39.0 84-85 32.55584265283242 35.0 33.0 36.0 26.0 37.0 86-87 32.27134853723196 35.0 33.0 36.0 26.0 37.0 88-89 32.05770035210861 35.0 33.0 35.0 26.0 37.0 90-91 31.901248590133157 35.0 33.0 35.0 26.0 36.0 92-93 31.737352484923935 35.0 33.0 35.0 25.5 36.0 94-95 31.548959534147976 35.0 33.0 35.0 25.0 36.0 96-97 31.376167770298096 35.0 33.0 35.0 25.0 36.0 98-99 31.18512392633965 35.0 33.0 35.0 24.0 35.0 100 31.06830165634806 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39966813051858807 1101 2 0.4071255987329785 1101 3 0.49741084126035773 1101 4 0.4035918791922839 1101 5 0.2963425298843205 1101 6 0.28523452708412833 1101 7 0.3046084333629224 1101 8 0.32706223325077843 1101 9 0.34867730363043137 1101 10-11 0.3304287792452243 1101 12-13 0.2898662614691645 1101 14-15 0.4807456110572943 1101 16-17 0.4733094693225155 1101 18-19 0.4752761005171422 1101 20-21 0.458135725366418 1101 22-23 0.48057411373364545 1101 24-25 0.5183008418320654 1101 26-27 0.5285173207194944 1101 28-29 0.4925284469987261 1101 30-31 0.5022773592481329 1101 32-33 0.5540008216910834 1101 34-35 0.5799267233245757 1101 36-37 0.6307508799018251 1101 38-39 0.6045978529478404 1101 40-41 0.6283414693444129 1101 42-43 0.5738321264304176 1101 44-45 0.5824386856396586 1101 46-47 0.6990800042790823 1101 48-49 0.7100693580464323 1101 50-51 0.6989032951278844 1101 52-53 0.698069688813014 1101 54-55 0.616317848058209 1101 56-57 0.598275520632626 1101 58-59 0.6335696823038148 1101 60-61 0.6681012545930969 1101 62-63 0.645342507076073 1101 64-65 0.6476035974931662 1101 66-67 0.6667265309844552 1101 68-69 0.6198211154574125 1101 70-71 0.6054181281645086 1101 72-73 0.5912859051121373 1101 74-75 0.572284926795966 1101 76-77 0.5078772999677668 1101 78-79 0.4428369214591683 1101 80-81 0.46763735652223204 1101 82-83 0.49788788596367084 1101 84-85 0.5459628778287922 1101 86-87 0.6304426122795732 1101 88-89 0.59569213497997 1101 90-91 0.6059071620859839 1101 92-93 0.6361714264145455 1101 94-95 0.5630388746372361 1101 96-97 0.642514685175307 1101 98-99 0.6854280484122022 1101 100 0.7516236321694798 1102 1 0.26677865917251964 1102 2 0.3634732363890443 1102 3 0.4280144108537378 1102 4 0.369942290220024 1102 5 0.3153061845102485 1102 6 0.294443642770986 1102 7 0.3158942928731392 1102 8 0.29899758164043533 1102 9 0.367226346382175 1102 10-11 0.32198820925848537 1102 12-13 0.34441030018408014 1102 14-15 0.41948489549330503 1102 16-17 0.4689253882237878 1102 18-19 0.5065567463940184 1102 20-21 0.4225334565179111 1102 22-23 0.4070110692636959 1102 24-25 0.41077649927643023 1102 26-27 0.39996299384151257 1102 28-29 0.4140505472055054 1102 30-31 0.49719885209023573 1102 32-33 0.5166556736772137 1102 34-35 0.510933429720474 1102 36-37 0.5324737715786938 1102 38-39 0.5140222045787652 1102 40-41 0.5163545668536145 1102 42-43 0.5562900711583438 1102 44-45 0.5990234358313842 1102 46-47 0.6155835873158324 1102 48-49 0.6597062139772873 1102 50-51 0.6686142108606177 1102 52-53 0.613687240947705 1102 54-55 0.551832113538822 1102 56-57 0.6149196171428315 1102 58-59 0.5968432692670405 1102 60-61 0.6030247913740823 1102 62-63 0.607905691661756 1102 64-65 0.6168438666013145 1102 66-67 0.6345522278329341 1102 68-69 0.5989480932470457 1102 70-71 0.6442276640103302 1102 72-73 0.6365222579403493 1102 74-75 0.6179561826212492 1102 76-77 0.5736371136932163 1102 78-79 0.5754690271453882 1102 80-81 0.5573845224725176 1102 82-83 0.5248915617687189 1102 84-85 0.5744314430840234 1102 86-87 0.6126476659597344 1102 88-89 0.5679180268576047 1102 90-91 0.5800007250883681 1102 92-93 0.5922654982797688 1102 94-95 0.627134620966217 1102 96-97 0.6298504720165781 1102 98-99 0.6281877629326651 1102 100 0.671713390402104 1103 1 0.3292354678947369 1103 2 0.3290209016211456 1103 3 0.40415789940120206 1103 4 0.35138203102232524 1103 5 0.2598127407343256 1103 6 0.2650017174797128 1103 7 0.28287643102681415 1103 8 0.2847006262072611 1103 9 0.34123141778959365 1103 10-11 0.28246775183378503 1103 12-13 0.2700759028359627 1103 14-15 0.36973353020471933 1103 16-17 0.3848168102445584 1103 18-19 0.37078057577296164 1103 20-21 0.35721595641800974 1103 22-23 0.36391059060000686 1103 24-25 0.37299061990627536 1103 26-27 0.3786756464699863 1103 28-29 0.39236467083757987 1103 30-31 0.3916993714918391 1103 32-33 0.4107160088160029 1103 34-35 0.43641969772788514 1103 36-37 0.43705389534324723 1103 38-39 0.4317732810801971 1103 40-41 0.4442673583749581 1103 42-43 0.4441848311828167 1103 44-45 0.47845775169686533 1103 46-47 0.4838874202675143 1103 48-49 0.44980394786952616 1103 50-51 0.5152664277309285 1103 52-53 0.4594621102565313 1103 54-55 0.3976164797378203 1103 56-57 0.4708284511500622 1103 58-59 0.44463170905324034 1103 60-61 0.38688873593020645 1103 62-63 0.3622676490531944 1103 64-65 0.4452311213934834 1103 66-67 0.46804799259435725 1103 68-69 0.4930324173942182 1103 70-71 0.45735224196887003 1103 72-73 0.4954113113890415 1103 74-75 0.4119221547532135 1103 76-77 0.4470792492776283 1103 78-79 0.4211485701427762 1103 80-81 0.38432952642411067 1103 82-83 0.3954002560525254 1103 84-85 0.4542236963992323 1103 86-87 0.5205604067629181 1103 88-89 0.4971095116667712 1103 90-91 0.41182454361566556 1103 92-93 0.40497956550236225 1103 94-95 0.3996400806568907 1103 96-97 0.4488633261084729 1103 98-99 0.49889058182070656 1103 100 0.5318703042164294 1104 1 0.3059651659793339 1104 2 0.28673980999022675 1104 3 0.3546675568425677 1104 4 0.2970218695219202 1104 5 0.23588015390835437 1104 6 0.23893773322348721 1104 7 0.16217673678552558 1104 8 0.22695775604055513 1104 9 0.26073450097172923 1104 10-11 0.2909321085582448 1104 12-13 0.27705640350671246 1104 14-15 0.36464747818104826 1104 16-17 0.3248533257815325 1104 18-19 0.36500550628049666 1104 20-21 0.36140809282068176 1104 22-23 0.35856186884693386 1104 24-25 0.32647093084899126 1104 26-27 0.3967138585899903 1104 28-29 0.3704386103309929 1104 30-31 0.36108154469443576 1104 32-33 0.35254540146690516 1104 34-35 0.2988755382230863 1104 36-37 0.3400776442554587 1104 38-39 0.366741693596893 1104 40-41 0.381066890189544 1104 42-43 0.37810647309817114 1104 44-45 0.33173071467998483 1104 46-47 0.41608287020734736 1104 48-49 0.35908068591239584 1104 50-51 0.39464434728785847 1104 52-53 0.3906423039497682 1104 54-55 0.3062081086281623 1104 56-57 0.32774505369380336 1104 58-59 0.3420703480816414 1104 60-61 0.3416526850034671 1104 62-63 0.3773085279287045 1104 64-65 0.3962028742611423 1104 66-67 0.3268393031316137 1104 68-69 0.3184213395521027 1104 70-71 0.35456287015149 1104 72-73 0.3558533127069907 1104 74-75 0.3681721131105533 1104 76-77 0.3358288765250137 1104 78-79 0.31456311817971994 1104 80-81 0.35288523293149154 1104 82-83 0.3271480534603626 1104 84-85 0.3494248081161473 1104 86-87 0.3631664817977125 1104 88-89 0.32369484174886054 1104 90-91 0.31948213367147815 1104 92-93 0.3192076446014873 1104 94-95 0.40882969006090164 1104 96-97 0.4193654066165351 1104 98-99 0.5556864693944554 1104 100 0.5820013018771277 1105 1 0.2695023016431257 1105 2 0.2620412528100182 1105 3 0.35394014326650236 1105 4 0.3030604092720921 1105 5 0.18866837894950805 1105 6 0.24942964762263387 1105 7 0.24091015404126637 1105 8 0.2472924127656455 1105 9 0.26338309204367505 1105 10-11 0.27967453133349807 1105 12-13 0.2686422464427167 1105 14-15 0.3506511578556868 1105 16-17 0.3151483775874837 1105 18-19 0.3364279910292183 1105 20-21 0.32868852276161675 1105 22-23 0.31921539842958424 1105 24-25 0.3224327784329901 1105 26-27 0.34294974510108034 1105 28-29 0.3974993249625456 1105 30-31 0.40783423182526235 1105 32-33 0.37200293995823586 1105 34-35 0.2823754898063129 1105 36-37 0.33405895538163577 1105 38-39 0.35729800230222253 1105 40-41 0.33545645153359516 1105 42-43 0.28257911762223387 1105 44-45 0.3855194238888586 1105 46-47 0.354276440460076 1105 48-49 0.3913809667296704 1105 50-51 0.4633527814894691 1105 52-53 0.41012986083946856 1105 54-55 0.37654847853229256 1105 56-57 0.38191122005382283 1105 58-59 0.4078815226725254 1105 60-61 0.39977605093743307 1105 62-63 0.4327903716393635 1105 64-65 0.4188298714529637 1105 66-67 0.4224150753791136 1105 68-69 0.4524002298396965 1105 70-71 0.4260673729112412 1105 72-73 0.420041944449018 1105 74-75 0.4319666323320135 1105 76-77 0.42002244677775025 1105 78-79 0.42189951790858515 1105 80-81 0.36828263779253234 1105 82-83 0.3809432505166228 1105 84-85 0.40585988122546723 1105 86-87 0.490435872714734 1105 88-89 0.4644802586172645 1105 90-91 0.4218434196209273 1105 92-93 0.4112513165294409 1105 94-95 0.4573981029591323 1105 96-97 0.44928338860549744 1105 98-99 0.4748139991707667 1105 100 0.5150554318277436 1106 1 0.16854986411296125 1106 2 0.19673244948324609 1106 3 0.3063616336314787 1106 4 0.2411007315533027 1106 5 0.18310360710827212 1106 6 0.1992276649248268 1106 7 0.18610675300907786 1106 8 0.2024461646685154 1106 9 0.22588084572874578 1106 10-11 0.24315735909126346 1106 12-13 0.15675597309773792 1106 14-15 0.29719480319783287 1106 16-17 0.28410902066475074 1106 18-19 0.2736310504730213 1106 20-21 0.2350767825173108 1106 22-23 0.23886931741372308 1106 24-25 0.228188097186937 1106 26-27 0.24810914063814948 1106 28-29 0.25465136892348283 1106 30-31 0.262439548722746 1106 32-33 0.29323072743085277 1106 34-35 0.28730453595787253 1106 36-37 0.294456423172079 1106 38-39 0.32331348516710534 1106 40-41 0.28509150889816226 1106 42-43 0.3105328918026089 1106 44-45 0.29691867387957416 1106 46-47 0.34001568228237744 1106 48-49 0.30477661519066146 1106 50-51 0.3453582023486419 1106 52-53 0.31816713852855827 1106 54-55 0.24396633023837921 1106 56-57 0.28974933686220083 1106 58-59 0.31136988267697774 1106 60-61 0.31711982994459476 1106 62-63 0.2862356246746742 1106 64-65 0.2611518927651062 1106 66-67 0.2858592145392578 1106 68-69 0.33517229385039116 1106 70-71 0.266780512925358 1106 72-73 0.2773235692216005 1106 74-75 0.255817150283967 1106 76-77 0.22642266212695006 1106 78-79 0.26733348367548615 1106 80-81 0.23486226130273025 1106 82-83 0.24530121286764484 1106 84-85 0.2508576534751228 1106 86-87 0.3350770563927483 1106 88-89 0.33443722590646274 1106 90-91 0.30053331677312656 1106 92-93 0.3136641557972517 1106 94-95 0.25103846979535405 1106 96-97 0.2936725394613262 1106 98-99 0.3239455478285471 1106 100 0.4071435764829978 1107 1 0.20758515402225086 1107 2 0.20066039203939212 1107 3 0.30719293280094107 1107 4 0.2477336352381201 1107 5 0.18869058529261196 1107 6 0.16085950159577322 1107 7 0.16158000874728629 1107 8 0.19875044607515946 1107 9 0.18356440720152278 1107 10-11 0.1799224105544468 1107 12-13 0.1410917416698112 1107 14-15 0.22123471563431707 1107 16-17 0.18465076861699714 1107 18-19 0.1937269521681131 1107 20-21 0.24074654874230816 1107 22-23 0.23428725756954094 1107 24-25 0.25328446131468496 1107 26-27 0.2121898191222229 1107 28-29 0.29030586227543154 1107 30-31 0.26617361144013074 1107 32-33 0.26267909745470774 1107 34-35 0.22838314842959306 1107 36-37 0.31240877857370464 1107 38-39 0.25911710855187664 1107 40-41 0.24030672361626415 1107 42-43 0.23728929919389685 1107 44-45 0.2640204590855433 1107 46-47 0.3049606779873315 1107 48-49 0.30937607473824613 1107 50-51 0.3893550167814297 1107 52-53 0.35236192811809275 1107 54-55 0.3344845894907209 1107 56-57 0.333497031446484 1107 58-59 0.3502288723857845 1107 60-61 0.32623238029486856 1107 62-63 0.32250222051897026 1107 64-65 0.3396376363442215 1107 66-67 0.32798558784728726 1107 68-69 0.35069261335716817 1107 70-71 0.38318276527827067 1107 72-73 0.3677985541340618 1107 74-75 0.3429536053436095 1107 76-77 0.2912806413214355 1107 78-79 0.2617893839742891 1107 80-81 0.290981972701573 1107 82-83 0.3113430196585938 1107 84-85 0.2870609842137952 1107 86-87 0.3016264954817345 1107 88-89 0.26400229090074134 1107 90-91 0.294824144808274 1107 92-93 0.2789184595698053 1107 94-95 0.2972734762712079 1107 96-97 0.2785314327250319 1107 98-99 0.3554646157082644 1107 100 0.3250341782320376 1108 1 0.10472637968400278 1108 2 0.1428742291314471 1108 3 0.23835993137511124 1108 4 0.15827461641618612 1108 5 0.12852937588113633 1108 6 0.1768849246445754 1108 7 0.15438667809615225 1108 8 0.16884257776303713 1108 9 0.13041036003418327 1108 10-11 0.1463131001981921 1108 12-13 0.14778864544693704 1108 14-15 0.17889740923066455 1108 16-17 0.18864325398417492 1108 18-19 0.18368215938445331 1108 20-21 0.14788640384770702 1108 22-23 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-0.6116211062011843 2215 30-31 -0.553755684423507 2215 32-33 -0.6146323803126563 2215 34-35 -0.7202968464393606 2215 36-37 -0.7311866761129622 2215 38-39 -0.6859562757311721 2215 40-41 -0.6700337257915336 2215 42-43 -0.6898571513122604 2215 44-45 -0.773388591930825 2215 46-47 -0.7428451280660013 2215 48-49 -0.7329112190739835 2215 50-51 -0.7506252338132171 2215 52-53 -0.8934872487091994 2215 54-55 -0.800563267318914 2215 56-57 -0.716634946518063 2215 58-59 -0.7374563514938473 2215 60-61 -0.9227413972350433 2215 62-63 -0.8684356015809271 2215 64-65 -0.8193036781515417 2215 66-67 -0.68173928278663 2215 68-69 -0.6402731532963202 2215 70-71 -0.7753705762176395 2215 72-73 -0.7028553052020143 2215 74-75 -0.794261925802715 2215 76-77 -0.728021832806597 2215 78-79 -0.7206978428937703 2215 80-81 -0.7362813388398379 2215 82-83 -0.7224552398933497 2215 84-85 -0.672180301269492 2215 86-87 -0.6550161124995597 2215 88-89 -0.6400202842147635 2215 90-91 -0.6796852987784909 2215 92-93 -0.7834904505180695 2215 94-95 -0.8168749674022422 2215 96-97 -0.92134297493687 2215 98-99 -0.9069737922783325 2215 100 -0.9510015396864979 2216 1 -0.6095121911104933 2216 2 -1.0458308699521268 2216 3 -0.7238228575860113 2216 4 -1.1865286785369449 2216 5 -0.6525694727128908 2216 6 -0.839642816145485 2216 7 -0.6104044239638498 2216 8 -0.5533861891098866 2216 9 -0.6179536513493318 2216 10-11 -0.8602171992717942 2216 12-13 -0.6680917689025563 2216 14-15 -0.9196727978135399 2216 16-17 -0.9102591245438063 2216 18-19 -0.9436299799852819 2216 20-21 -0.7644512896850983 2216 22-23 -0.7785365197289877 2216 24-25 -0.8120514500410039 2216 26-27 -1.004248890875047 2216 28-29 -1.2663557475012226 2216 30-31 -1.3542160665549687 2216 32-33 -1.0735332919258127 2216 34-35 -1.018737894677912 2216 36-37 -0.9998447104325123 2216 38-39 -1.144404469991045 2216 40-41 -0.991408851109199 2216 42-43 -1.5522674296923427 2216 44-45 -1.269957131950875 2216 46-47 -1.060235921006246 2216 48-49 -1.0094672856147469 2216 50-51 -1.2005074060638137 2216 52-53 -1.0799707359209734 2216 54-55 -1.2500644796944727 2216 56-57 -1.3022081320348349 2216 58-59 -1.0567885540538242 2216 60-61 -1.3185751852092054 2216 62-63 -1.347375868769845 2216 64-65 -1.2956841950432008 2216 66-67 -1.1819421283719507 2216 68-69 -1.5060942288133248 2216 70-71 -1.2673486543692363 2216 72-73 -1.0597415167615551 2216 74-75 -1.1540606481453963 2216 76-77 -1.2932350127277914 2216 78-79 -1.3026776346915767 2216 80-81 -1.1061359470326977 2216 82-83 -1.2070456667894334 2216 84-85 -1.2789338101641334 2216 86-87 -1.4434575629203934 2216 88-89 -1.2890005597970564 2216 90-91 -1.1595521473014898 2216 92-93 -1.2197896041866692 2216 94-95 -1.3418209933750553 2216 96-97 -1.3847311041481234 2216 98-99 -1.4484920536851575 2216 100 -1.4854748324518816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 472.0 3 40.0 4 57.0 5 158.0 6 491.0 7 1358.0 8 2816.0 9 4551.0 10 6405.0 11 9334.0 12 13213.0 13 18724.0 14 22015.0 15 28075.0 16 37494.0 17 53241.0 18 53933.0 19 29986.0 20 21708.0 21 21070.0 22 21836.0 23 23974.0 24 27476.0 25 34017.0 26 42802.0 27 55386.0 28 72136.0 29 94871.0 30 123704.0 31 159604.0 32 205095.0 33 266325.0 34 361230.0 35 520297.0 36 838101.0 37 1535548.0 38 2154064.0 39 849570.0 40 3204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.22027521187729 20.757522047677433 15.008802468660216 23.013400271785056 2 15.404899258537757 20.91400819070846 39.984919016518695 23.69617353423509 3 17.855096585856543 25.675115822658462 31.086438573158183 25.38334901832681 4 12.594360987638298 17.830842607313198 38.0397391388988 31.535057266149703 5 12.514189661902018 37.673832675480924 35.51266144973654 14.29931621288051 6 29.87306440424945 38.404301558665566 17.51117772396379 14.211456313121198 7 25.98828084845693 33.40593367115262 22.523790307997494 18.081995172392965 8 24.68312104367155 36.181684570673916 20.47162306347068 18.663571322183852 9 24.56606989340421 16.90263736172569 22.022364478993506 36.50892826587659 10-11 23.491230210174997 27.292500850035793 28.714053661596438 20.502215278192768 12-13 24.42883275877848 24.854641553732524 29.159486351329946 21.557039336159054 14-15 21.625149606987158 26.791858424378685 27.13701906530649 24.44597290332767 16-17 20.30588066625177 30.10178652052576 27.933569783499156 21.658763029723318 18-19 20.211038319211873 28.19824429205661 31.09113355951696 20.499583829214554 20-21 21.28880463643162 27.396281049639626 30.977974772052352 20.3369395418764 22-23 20.959361483442418 27.278526948565286 31.103681552674157 20.658430015318142 24-25 20.78706638938367 27.344501133661925 31.0734198894247 20.79501258752971 26-27 20.59340859623086 27.398497688926692 31.089636355787974 20.918457359054475 28-29 20.614235670375074 27.39223744576354 31.241781569144745 20.751745314716644 30-31 20.941888709778507 27.63038781628266 30.85531446769768 20.572409006241152 32-33 20.58658501483042 27.48038549563377 31.175970643397743 20.757058846138065 34-35 20.756526025872976 27.223013745367258 31.096798304361684 20.923661924398083 36-37 21.05406772105241 27.420910375051477 30.65765743226838 20.86736447162773 38-39 20.91871661511144 27.435273160607444 30.810048142553498 20.835962081727622 40-41 20.820192832062613 27.27640753029958 30.86195120515826 21.041448432479548 42-43 20.81980394797716 27.341908573092255 30.690712579531652 21.147574899398926 44-45 20.950371779667094 27.458392319487462 30.472496756382654 21.11873914446279 46-47 21.007330335382708 27.654623229005672 30.085518202951082 21.252528232660534 48-49 21.037805884879162 27.60657660024829 29.790977396631046 21.564640118241503 50-51 21.055383445541516 27.65766948767503 29.58834934390718 21.698597722876276 52-53 20.99362217137059 27.765163530294913 29.554380319043094 21.686833979291404 54-55 21.063478715920304 27.992459796839174 29.39228046942457 21.551781017815948 56-57 20.866871885119494 27.752317392672204 29.3992868643641 21.981523857844202 58-59 20.739840544231445 27.4522076671249 29.701880841780238 22.106070946863415 60-61 20.942542246746694 27.6266106120504 29.441876153122333 21.988970988080574 62-63 20.693578655241428 27.60216276587843 29.518564094773126 22.18569448410702 64-65 20.665079933179346 27.4272167786372 29.62546184846198 22.282241439721474 66-67 20.631344238766534 27.464705204474605 29.40453679951768 22.499413757241186 68-69 20.937071943944694 27.64487520126372 29.126303199180853 22.291749655610733 70-71 20.859424754883122 27.422031657497858 29.109367297259496 22.609176290359525 72-73 20.714669135475678 27.53218307470165 29.13349107336026 22.619656716462412 74-75 20.806024488549372 27.558756820540754 28.919228905080008 22.71598978582987 76-77 20.81089850242035 27.633552192975692 28.729848836867145 22.825700467736816 78-79 20.849541994266932 27.74535595493008 28.688446760094493 22.716655290708502 80-81 20.73719977071314 27.66455882112747 28.64591772687642 22.95232368128297 82-83 20.64495727187078 27.586852356826675 28.73514004279813 23.033050328504416 84-85 20.681691765029495 27.52937014648356 28.506830295262837 23.28210779322411 86-87 20.751412200149304 27.63729844299886 28.438360770617887 23.172928586233944 88-89 20.6620443880303 27.5464953294349 28.440404195957754 23.35105608657705 90-91 20.56572671408332 27.450267056510125 28.336300843937074 23.647705385469482 92-93 20.601817344180326 27.529946201447853 28.1901068841458 23.678129570226023 94-95 20.530882780048763 27.330486297627942 28.32838767337113 23.810243248952162 96-97 20.51629936348749 27.3765646265073 28.258501881097136 23.848634128908074 98-99 20.494061835311815 27.39111776794462 28.173157435819363 23.9416629609242 100 20.588465195503677 27.37063157342107 28.063227990078172 23.977675240997083 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 963.0 1 820.0 2 1633.0 3 2609.5 4 1735.0 5 941.0 6 2091.5 7 5488.5 8 8999.0 9 10267.0 10 8210.0 11 5428.0 12 5017.5 13 5570.5 14 6289.5 15 7064.0 16 7887.5 17 9002.0 18 10141.5 19 11452.0 20 13712.0 21 16567.0 22 20527.5 23 26762.0 24 34698.5 25 43944.0 26 56580.0 27 73433.0 28 94595.5 29 116038.0 30 134723.5 31 153831.5 32 172304.5 33 188828.0 34 205708.0 35 222015.5 36 236331.5 37 249449.0 38 260622.0 39 268423.5 40 274595.0 41 277907.0 42 276372.0 43 275890.0 44 274514.5 45 270731.5 46 267101.5 47 260228.5 48 252536.5 49 248339.0 50 241796.5 51 230630.0 52 218666.0 53 208671.5 54 199077.0 55 186660.0 56 170283.5 57 150446.0 58 133397.0 59 119392.0 60 102462.5 61 83662.0 62 68401.0 63 54956.0 64 42829.0 65 33287.0 66 25096.5 67 18828.5 68 13903.0 69 10157.0 70 7415.5 71 5348.5 72 3776.0 73 2448.5 74 1665.0 75 1093.0 76 667.0 77 380.0 78 227.5 79 133.5 80 80.5 81 43.0 82 22.5 83 13.5 84 8.5 85 3.5 86 3.5 87 4.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05978444673655604 2 3.8888408545027786E-4 3 1.2962802848342595E-5 4 0.11836335280821624 5 0.0017888667930712782 6 0.0019962716386447595 7 0.0 8 0.0 9 6.481401424171297E-5 10-11 0.0 12-13 3.888840854502779E-5 14-15 2.592560569668519E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.6851643702845372E-4 30-31 2.527746555426806E-4 32-33 6.4814014241712976E-6 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.0740484557348152E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.6203503560428245E-4 80-81 3.499956769052501E-4 82-83 3.240700712085649E-4 84-85 0.0 86-87 0.0 88-89 5.185121139337038E-5 90-91 3.2407007120856486E-5 92-93 6.09251733872102E-4 94-95 5.379563182062177E-4 96-97 0.0 98-99 3.2407007120856486E-5 100 9.851730164740372E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7714381.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.62133594920436 #Duplication Level Percentage of deduplicated Percentage of total 1 75.20964862914862 44.08890078910938 2 14.618194840046254 17.13876221378554 3 4.654385238525823 8.185388421139196 4 1.9763033431656134 4.634141688689885 5 0.9914867147783393 2.906113789809699 6 0.6136732354980562 2.1584606940700053 7 0.3934075643475675 1.614345389620389 8 0.2637218042612935 1.2367779587785277 9 0.1950908147346098 1.0292835772045392 >10 1.0051919864525716 10.445295052412058 >50 0.05293985750639122 2.1250984945862856 >100 0.024363823189045824 2.545254895098544 >500 0.0010615211880960185 0.42934922062286646 >1k 4.4218930330749664E-4 0.5032703274173944 >5k 2.210946360857739E-5 0.09632513335931274 >10k+ 6.632839082573216E-5 0.8632323542963276 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26283 0.34070134726298845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25284 0.3277515072174942 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15036 0.19490870362767926 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.592560569668519E-5 0.0 0.0 0.0 6 0.0 2.592560569668519E-5 0.0 0.0 0.0 7 0.0 2.592560569668519E-5 0.0 0.0 0.0 8 0.0 2.592560569668519E-5 0.0 0.0 0.0 9 0.0 2.592560569668519E-5 0.0 1.2962802848342595E-5 0.0 10-11 0.0 2.592560569668519E-5 0.0 3.240700712085649E-5 0.0 12-13 0.0 2.592560569668519E-5 0.0 8.425821851422687E-5 0.0 14-15 0.0 3.240700712085649E-5 0.0 9.722102136256947E-5 0.0 16-17 0.0 3.888840854502779E-5 0.0 1.1666522563508335E-4 0.0 18-19 0.0 3.888840854502779E-5 0.0 1.4907223275593986E-4 0.0 20-21 0.0 3.888840854502779E-5 0.0 2.333304512701667E-4 0.0 22-23 0.0 3.888840854502779E-5 0.0 6.351773395687872E-4 0.0 24-25 0.0 3.888840854502779E-5 0.0 0.0015166479332560838 0.0 26-27 0.0 3.888840854502779E-5 0.0 0.003201812303540621 0.0 28-29 0.0 3.888840854502779E-5 0.0 0.010603572729944243 0.0 30-31 0.0 3.888840854502779E-5 0.0 0.03290607503051768 0.0 32-33 0.0 3.888840854502779E-5 0.0 0.08318230587781444 0.0 34-35 0.0 5.185121139337038E-5 0.0 0.16348038812187266 0.0 36-37 0.0 7.777681709005558E-5 0.0 0.2956880143721188 0.0 38-39 0.0 1.0370242278674076E-4 0.0 0.4781135388568441 0.0 40-41 0.0 1.1666522563508335E-4 0.0 0.6712009686843312 0.0 42-43 0.0 1.4259083133176856E-4 0.0 0.873355879104234 0.0 44-45 0.0 1.4259083133176856E-4 0.0 1.1287684650265524 0.0 46-47 0.0 1.4907223275593986E-4 0.0 1.4395257377098694 0.0 48-49 1.2962802848342595E-5 1.6851643702845375E-4 0.0 1.7655402293456857 0.0 50-51 1.2962802848342595E-5 1.6851643702845375E-4 0.0 2.095411154828884 0.0 52-53 1.2962802848342595E-5 1.9444204272513893E-4 0.0 2.423084885229288 0.0 54-55 1.2962802848342595E-5 1.9444204272513893E-4 0.0 2.7940608585445803 0.0 56-57 1.2962802848342595E-5 1.9444204272513893E-4 0.0 3.2307841679066667 0.0 58-59 1.2962802848342595E-5 1.9444204272513893E-4 0.0 3.706324590398115 0.0 60-61 1.2962802848342595E-5 1.9444204272513893E-4 0.0 4.188709372793488 0.0 62-63 1.2962802848342595E-5 1.9444204272513893E-4 0.0 4.662396114477623 0.0 64-65 1.2962802848342595E-5 1.9444204272513893E-4 0.0 5.167465801857595 0.0 66-67 1.2962802848342595E-5 2.0740484557348152E-4 0.0 5.738289306685786 0.0 68-69 1.2962802848342595E-5 2.2036764842182411E-4 0.0 6.353640039298034 0.0 70-71 1.2962802848342595E-5 2.333304512701667E-4 0.0 7.003646825325324 0.0 72-73 1.2962802848342595E-5 2.5277465554268057E-4 0.0 7.658631327646379 0.0 74-75 1.2962802848342595E-5 2.851816626635371E-4 0.0 8.3111399346234 0.0 76-77 1.2962802848342595E-5 2.981444655118797E-4 0.0 8.993242620503187 0.0 78-79 1.2962802848342595E-5 3.240700712085649E-4 0.0 9.739051000981155 0.0 80-81 1.2962802848342595E-5 3.240700712085649E-4 0.0 10.522470953923587 0.0 82-83 1.2962802848342595E-5 3.240700712085649E-4 0.0 11.308139953160207 0.0 84-85 1.2962802848342595E-5 3.305514726327362E-4 0.0 12.070748126129626 0.0 86-87 1.2962802848342595E-5 3.4351427548107876E-4 0.0 12.855509729166865 0.0 88 1.2962802848342595E-5 3.499956769052501E-4 0.0 13.476959460519256 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15030 0.0 56.392292 1 GTATCAA 26720 0.0 41.730297 1 GTGGTAT 5880 0.0 33.473812 1 ATCAACG 34080 0.0 32.28307 3 TCAACGC 34335 0.0 32.05702 4 TATCAAC 34665 0.0 31.928526 2 CAACGCA 35580 0.0 30.988455 5 AACGCAG 37285 0.0 29.609013 6 TGGTATC 6645 0.0 28.751719 2 ACGCAGA 42785 0.0 25.703587 7 CGCAGAG 42700 0.0 25.534626 8 GTACATG 23545 0.0 23.55878 1 GCAGAGT 46210 0.0 23.188267 9 TACATGG 23730 0.0 22.468027 2 ATAACGC 630 0.0 21.661442 3 GAGTACT 32285 0.0 21.267616 12-13 ACATGGG 24405 0.0 21.094461 3 CAGAGTA 44705 0.0 20.610104 10-11 AGAGTAC 42445 0.0 20.406487 10-11 AGTACTT 33540 0.0 19.45594 12-13 >>END_MODULE