FastQCFastQC Report
Wed 25 May 2016
SRR2049520_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049520_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8866337
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99650.041.797321
GTATCAA153600.034.778481
TCAACGC163500.031.664914
ATCAACG167000.031.0319063
CAACGCA177400.029.1549975
TATCAAC185600.028.3354852
AACGCAG190850.027.1803916
GTACATG293650.024.7434161
TACATGG298500.023.0511932
ACGCAGA223550.022.7009587
CGCAGAG231750.021.7084878
ACATGGG307150.021.6358573
GTGGTAT57000.020.198031
CATGGGG225300.018.59684
GCAGAGT266400.017.950869
GAGTACA227150.017.2325691
AGAGTAC225150.016.1056610-11
CAGAGTA250500.015.99292110-11
AGTACAT235350.015.2811692
TGGTATC68400.014.9585472
Produced by FastQC (version 0.11.5)