##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049520_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8866337 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.710803458068423 31.0 31.0 34.0 27.0 34.0 2 30.941603731056016 31.0 31.0 34.0 28.0 34.0 3 31.03560478244849 31.0 31.0 34.0 27.0 34.0 4 34.61528227496879 37.0 35.0 37.0 32.0 37.0 5 34.59534664653509 37.0 35.0 37.0 32.0 37.0 6 34.641018156652514 37.0 35.0 37.0 32.0 37.0 7 30.721699502286008 37.0 33.0 37.0 2.0 37.0 8 32.503292622421185 37.0 33.0 37.0 17.0 37.0 9 35.2642435089034 39.0 34.0 39.0 28.0 39.0 10-11 35.801016925027774 39.0 36.0 39.0 30.0 39.0 12-13 35.94715106136841 39.0 37.0 39.0 30.0 39.0 14-15 37.130036112996834 40.0 37.0 41.0 31.0 41.0 16-17 37.140151282316474 40.0 37.0 41.0 31.0 41.0 18-19 37.13333082196176 40.0 37.0 41.0 31.0 41.0 20-21 37.19039006751041 40.0 37.0 41.0 31.0 41.0 22-23 37.151282542046395 40.0 37.0 41.0 31.0 41.0 24-25 37.1212802987299 40.0 37.0 41.0 31.0 41.0 26-27 37.14111537831238 40.0 37.0 41.0 31.0 41.0 28-29 37.12453530697062 40.0 37.0 41.0 31.0 41.0 30-31 37.05001637090943 40.0 37.0 41.0 30.0 41.0 32-33 37.00292646219064 40.0 37.0 41.0 30.0 41.0 34-35 36.94747554711715 40.0 37.0 41.0 30.0 41.0 36-37 36.785368974808875 40.0 37.0 41.0 30.0 41.0 38-39 36.677234803955685 40.0 36.5 41.0 30.0 41.0 40-41 36.60229974340024 40.0 36.0 41.0 30.0 41.0 42-43 36.49038689821964 40.0 36.0 41.0 30.0 41.0 44-45 36.37415129833211 40.0 36.0 41.0 30.0 41.0 46-47 36.216955209349706 40.0 35.5 41.0 29.0 41.0 48-49 36.12929601029151 39.5 35.0 41.0 29.0 41.0 50-51 35.98182220008105 39.0 35.0 41.0 29.0 41.0 52-53 35.854094481182024 39.0 35.0 41.0 28.0 41.0 54-55 35.72096774575566 39.0 35.0 41.0 28.0 41.0 56-57 35.50899125535156 39.0 35.0 41.0 28.0 41.0 58-59 35.317109309064165 39.0 35.0 40.5 28.0 41.0 60-61 35.08382384969126 38.0 34.0 40.0 27.5 41.0 62-63 34.83591724519381 38.0 34.0 40.0 27.0 41.0 64-65 34.4694019074619 37.0 34.0 40.0 26.5 41.0 66-67 31.00718301142851 36.0 30.0 39.5 6.5 41.0 68-69 31.354284469448885 35.5 30.0 39.0 11.0 41.0 70-71 31.506259687625228 35.0 30.0 39.0 15.5 41.0 72-73 32.71606814629311 35.0 31.5 39.0 23.5 40.0 74-75 32.79044192658141 35.0 33.0 37.5 25.0 40.0 76-77 32.49525170315543 35.0 33.0 37.0 24.5 39.0 78-79 29.127534854585384 35.0 29.0 36.0 6.5 39.0 80-81 30.926533527881922 35.0 29.5 36.0 19.0 38.5 82-83 31.343674563689603 35.0 32.0 36.0 21.0 37.0 84-85 29.543459999321026 34.5 30.0 35.5 11.0 37.0 86-87 29.18853073146216 34.0 28.0 35.0 11.5 36.5 88-89 30.430827578514105 34.0 30.5 35.0 18.5 36.0 90-91 30.589974642290272 35.0 31.5 35.0 18.0 36.0 92-93 30.45056182727997 35.0 32.0 35.0 13.0 36.0 94-95 30.255454422722707 35.0 32.0 35.0 4.5 35.5 96-97 30.072316842908183 35.0 31.5 35.0 2.0 35.0 98-99 29.891219846482265 35.0 31.0 35.0 2.0 35.0 100 29.690965276866873 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.29333217293936187 1101 2 0.3174779730626085 1101 3 0.3389620042151087 1101 4 0.3471397799650475 1101 5 0.23684545459047257 1101 6 0.2821033763392009 1101 7 -12.15367749430623 1101 8 -6.559737911354691 1101 9 -2.222587886933816 1101 10-11 -0.16822427456709477 1101 12-13 0.3350905056589468 1101 14-15 0.5427904531524206 1101 16-17 0.5812994781537668 1101 18-19 0.5856071000227203 1101 20-21 0.5241728044623954 1101 22-23 0.5551160891619062 1101 24-25 0.5759561067675065 1101 26-27 0.5570956444120228 1101 28-29 0.5204378072102998 1101 30-31 0.5915359354037477 1101 32-33 0.5936070559268671 1101 34-35 0.5855158759602332 1101 36-37 0.6464016842954976 1101 38-39 0.6491679928883514 1101 40-41 0.6738617885171934 1101 42-43 0.6157197892743653 1101 44-45 0.5832269332925435 1101 46-47 0.5890201461952671 1101 48-49 0.5431966456216202 1101 50-51 0.6295476450404252 1101 52-53 0.6160048664654951 1101 54-55 0.5354560101888239 1101 56-57 0.5183252799723732 1101 58-59 0.4874890273471806 1101 60-61 0.49041354998614395 1101 62-63 0.497020050226638 1101 64-65 0.3286490582493684 1101 66-67 -10.194174534917348 1101 68-69 -8.06554511719304 1101 70-71 -6.5778177441960395 1101 72-73 -1.41578671322074 1101 74-75 -0.12420500197839601 1101 76-77 0.17891610524026902 1101 78-79 3.3241407456538994 1101 80-81 1.191246188783854 1101 82-83 0.5101596027861746 1101 84-85 -5.258532648022193 1101 86-87 -5.774921354337476 1101 88-89 -1.1784499749675206 1101 90-91 0.08421596785363761 1101 92-93 0.45466166955699805 1101 94-95 0.5017852538690661 1101 96-97 0.595972320414802 1101 98-99 0.5986200658052283 1101 100 0.6647554475082984 1102 1 0.18223200752482072 1102 2 0.22319559271762301 1102 3 0.19032805058832736 1102 4 0.23796538980327853 1102 5 0.20891206440202836 1102 6 0.27206681458713433 1102 7 -12.275029870490666 1102 8 -6.6525903355941765 1102 9 -2.3388874694029056 1102 10-11 -0.2641062672025427 1102 12-13 0.2775525530980545 1102 14-15 0.4239975991823073 1102 16-17 0.4260284030048638 1102 18-19 0.43098305112149404 1102 20-21 0.4589219973307834 1102 22-23 0.4209909055342749 1102 24-25 0.4787762951597756 1102 26-27 0.41912311390783685 1102 28-29 0.40271087254153315 1102 30-31 0.4074512136849293 1102 32-33 0.4095162679311173 1102 34-35 0.40151502408388495 1102 36-37 0.5276023712451376 1102 38-39 0.526219245040771 1102 40-41 0.45528662360791117 1102 42-43 0.43744158113410236 1102 44-45 0.39249471244153966 1102 46-47 0.42853225027091213 1102 48-49 0.3533090912714201 1102 50-51 0.4257807753261247 1102 52-53 0.4393325364620537 1102 54-55 0.397286935479471 1102 56-57 0.4370468179671292 1102 58-59 0.3610131940323811 1102 60-61 0.2991282240598139 1102 62-63 0.2644240498893282 1102 64-65 0.1450023945844805 1102 66-67 -10.400755498237057 1102 68-69 -6.809436990859133 1102 70-71 -6.3336068128726914 1102 72-73 -1.1842304759191329 1102 74-75 -0.24675170613370057 1102 76-77 -0.0678237349812818 1102 78-79 3.1130041350611677 1102 80-81 0.9692693662065857 1102 82-83 0.3453763709766591 1102 84-85 -5.503013020242612 1102 86-87 -6.024810552126045 1102 88-89 -1.4053061590949625 1102 90-91 -0.07767238274688282 1102 92-93 0.25361425636003787 1102 94-95 0.274615872456355 1102 96-97 0.3985062692329109 1102 98-99 0.3900956479159774 1102 100 0.4587145380571265 1103 1 0.3679999257837565 1103 2 0.37591507099978827 1103 3 0.3949700387968882 1103 4 0.38342168820202005 1103 5 0.354333996811107 1103 6 0.37712575669213777 1103 7 -12.037972768954681 1103 8 -6.501308337037646 1103 9 -2.155147618590206 1103 10-11 -0.1376210386370076 1103 12-13 0.4207085157727235 1103 14-15 0.6117587848594397 1103 16-17 0.606018232962171 1103 18-19 0.6208941551347635 1103 20-21 0.6082118397305223 1103 22-23 0.5973017337823308 1103 24-25 0.6858312535927524 1103 26-27 0.6124276439202987 1103 28-29 0.6718353487787994 1103 30-31 0.6392904781271582 1103 32-33 0.6774483637645758 1103 34-35 0.6356782931945233 1103 36-37 0.6796371709037885 1103 38-39 0.7417809679773839 1103 40-41 0.7114472290157323 1103 42-43 0.6664467011247837 1103 44-45 0.6415358570026157 1103 46-47 0.703112610569562 1103 48-49 0.6181824108579477 1103 50-51 0.6122895139964228 1103 52-53 0.547377087023925 1103 54-55 0.54988035793248 1103 56-57 0.6083581119599231 1103 58-59 0.5731377392848245 1103 60-61 0.5118189062037715 1103 62-63 0.45558522447231553 1103 64-65 0.3867701393511993 1103 66-67 -10.104951812136818 1103 68-69 -8.025829438640464 1103 70-71 -6.546209255936244 1103 72-73 -1.4381342510249482 1103 74-75 -0.24140816721372005 1103 76-77 0.09006262859771397 1103 78-79 3.248486847004422 1103 80-81 1.1466979849000474 1103 82-83 0.5173788695601864 1103 84-85 -5.258629637029749 1103 86-87 -5.739617900956166 1103 88-89 -1.2206963291290371 1103 90-91 0.07329455319391087 1103 92-93 0.44172593265138005 1103 94-95 0.5166041764455116 1103 96-97 0.6114507930321551 1103 98-99 0.6624659463182923 1103 100 0.7475425746958777 1104 1 0.19621873822326918 1104 2 0.2234159658300463 1104 3 0.22188090684627326 1104 4 0.21895387270043898 1104 5 0.21543953984078712 1104 6 0.27996997785249533 1104 7 -12.174894681776163 1104 8 -6.6365064107292895 1104 9 -2.3253880745225857 1104 10-11 -0.2941259488365091 1104 12-13 0.26613090893769 1104 14-15 0.40555974053661714 1104 16-17 0.428137133154479 1104 18-19 0.4607011681226254 1104 20-21 0.4460208324614712 1104 22-23 0.4641026995097448 1104 24-25 0.5508642174792158 1104 26-27 0.4848992484428791 1104 28-29 0.45430751012915493 1104 30-31 0.4322904047592644 1104 32-33 0.45590954474445766 1104 34-35 0.49809279683092456 1104 36-37 0.5559826755427508 1104 38-39 0.5672291262683231 1104 40-41 0.5861128333737611 1104 42-43 0.5379748531108177 1104 44-45 0.5288066119422012 1104 46-47 0.5081703034534542 1104 48-49 0.4533549401046315 1104 50-51 0.4645552805202229 1104 52-53 0.45051208457010006 1104 54-55 0.45414109665253477 1104 56-57 0.4841793372051342 1104 58-59 0.40768234977335993 1104 60-61 0.3733050356013834 1104 62-63 0.34264847387534303 1104 64-65 0.20532598388143697 1104 66-67 -10.314472562154382 1104 68-69 -8.249537155473483 1104 70-71 -6.679621916609367 1104 72-73 -1.5951684097700394 1104 74-75 -0.36256265488686523 1104 76-77 -0.05739086399967874 1104 78-79 3.115358532569836 1104 80-81 1.0323051461530248 1104 82-83 0.3243228312772004 1104 84-85 -5.458481598720706 1104 86-87 -5.986807864732789 1104 88-89 -1.3788831838248754 1104 90-91 -0.07941082921581355 1104 92-93 0.2764801017285379 1104 94-95 0.3113016118883358 1104 96-97 0.46471005118650766 1104 98-99 0.5232682031886497 1104 100 0.5750686867788062 1105 1 0.16067462638878638 1105 2 0.17006526559302415 1105 3 0.17623595570218598 1105 4 0.16973946757818226 1105 5 0.17177674884393923 1105 6 0.22716527639354922 1105 7 -12.209552722840087 1105 8 -6.622975808586958 1105 9 -2.336755946619853 1105 10-11 -0.34604457388877563 1105 12-13 0.19134113763005445 1105 14-15 0.3390396033154488 1105 16-17 0.3765873355606715 1105 18-19 0.3358490915206147 1105 20-21 0.4043106458173611 1105 22-23 0.43663241462316904 1105 24-25 0.4717385733563546 1105 26-27 0.4322980248769497 1105 28-29 0.41964878709514153 1105 30-31 0.4583681546358278 1105 32-33 0.46307950770226114 1105 34-35 0.5236699693916265 1105 36-37 0.573912892158944 1105 38-39 0.504084538076043 1105 40-41 0.5214953673319087 1105 42-43 0.4428463525590729 1105 44-45 0.4147793309857306 1105 46-47 0.3938624284410679 1105 48-49 0.37445271727273166 1105 50-51 0.44731626594941787 1105 52-53 0.43388598075149076 1105 54-55 0.4342989008107594 1105 56-57 0.37143311451203687 1105 58-59 0.3668382144142939 1105 60-61 0.29983580918874253 1105 62-63 0.2567111237167907 1105 64-65 0.10601082951471597 1105 66-67 -10.28742394331768 1105 68-69 -8.276587916034423 1105 70-71 -6.702403237259318 1105 72-73 -1.6630360832477464 1105 74-75 -0.46153744136498887 1105 76-77 -0.2007647099069132 1105 78-79 2.976763862578622 1105 80-81 0.9086424028377209 1105 82-83 0.2402178022928254 1105 84-85 -5.460738437819678 1105 86-87 -5.992520635153021 1105 88-89 -1.481929114730022 1105 90-91 -0.2000899111309984 1105 92-93 0.17617443089984164 1105 94-95 0.22270921409746336 1105 96-97 0.2908030941013564 1105 98-99 0.3327924024096518 1105 100 0.4027500182991339 1106 1 0.1575729685130085 1106 2 0.1833483955438524 1106 3 0.16971655484812231 1106 4 0.1398992560388308 1106 5 0.13376302161839249 1106 6 0.17390847492841033 1106 7 -12.131896727348956 1106 8 -6.653897528064004 1106 9 -2.5291194903659573 1106 10-11 -0.3924182202408275 1106 12-13 0.16745817438572175 1106 14-15 0.32251731416884155 1106 16-17 0.3556925811923435 1106 18-19 0.31997500110834665 1106 20-21 0.344153215447605 1106 22-23 0.37475074018255583 1106 24-25 0.4352638380719114 1106 26-27 0.37027925466072986 1106 28-29 0.3526452095785686 1106 30-31 0.34591781198049176 1106 32-33 0.35451971561839457 1106 34-35 0.404943655939384 1106 36-37 0.4846584851802618 1106 38-39 0.4380101988293674 1106 40-41 0.40297370953898337 1106 42-43 0.4375344995707735 1106 44-45 0.39376699324234465 1106 46-47 0.4353630044964518 1106 48-49 0.39186021454506204 1106 50-51 0.4307522737757381 1106 52-53 0.3776629024683231 1106 54-55 0.3992476834675003 1106 56-57 0.3870386964379904 1106 58-59 0.32001036651873704 1106 60-61 0.3629104182884646 1106 62-63 0.2596248267408612 1106 64-65 0.15146267620625053 1106 66-67 -10.294947344140372 1106 68-69 -8.262265846697233 1106 70-71 -6.769150352863001 1106 72-73 -1.6998882605945838 1106 74-75 -0.48450395595722995 1106 76-77 -0.1514572847229445 1106 78-79 2.993971252276637 1106 80-81 0.8755807671844664 1106 82-83 0.17553231102526112 1106 84-85 -5.497217936209289 1106 86-87 -5.996428406390997 1106 88-89 -1.4856676705329122 1106 90-91 -0.24126603534942603 1106 92-93 0.10089509424242138 1106 94-95 0.17006018757354724 1106 96-97 0.2449811024245534 1106 98-99 0.29861046264666413 1106 100 0.32780293952759365 1107 1 0.048979191183541104 1107 2 0.0961838881111916 1107 3 0.05315487414190301 1107 4 0.08941047151996884 1107 5 0.12904330686816934 1107 6 0.13107817872237604 1107 7 -12.13798724840991 1107 8 -6.696678838130811 1107 9 -2.52328560503161 1107 10-11 -0.513467691776043 1107 12-13 0.10172687942849024 1107 14-15 0.19836383021267778 1107 16-17 0.22384669154368453 1107 18-19 0.2429830587414017 1107 20-21 0.22959004331563904 1107 22-23 0.2505162146289237 1107 24-25 0.30945564746672716 1107 26-27 0.2206812112211196 1107 28-29 0.21998515877223213 1107 30-31 0.23099738567432127 1107 32-33 0.28279295362358425 1107 34-35 0.25221907296349855 1107 36-37 0.29585373273303617 1107 38-39 0.31873435417151796 1107 40-41 0.2885191663848019 1107 42-43 0.282292380082545 1107 44-45 0.2648823082412761 1107 46-47 0.3148263509378779 1107 48-49 0.2941034974994494 1107 50-51 0.2727388069408292 1107 52-53 0.25610842609096807 1107 54-55 0.24136371086744646 1107 56-57 0.19062080866962816 1107 58-59 0.21444247293280938 1107 60-61 0.22985547208537582 1107 62-63 0.12753124552400408 1107 64-65 -0.0014682892901234368 1107 66-67 -10.312812952433276 1107 68-69 -8.2671175097908 1107 70-71 -6.782555576316593 1107 72-73 -1.8300232365075324 1107 74-75 -0.5605661833347853 1107 76-77 -0.3068914275295569 1107 78-79 2.8764216541974506 1107 80-81 0.7822626947780584 1107 82-83 0.11005132446276633 1107 84-85 -5.536144108607729 1107 86-87 -6.005278649793478 1107 88-89 -1.558797040883313 1107 90-91 -0.31734648981745295 1107 92-93 0.06802809967165047 1107 94-95 0.16211753750653202 1107 96-97 0.25183064456020077 1107 98-99 0.25620802424874967 1107 100 0.3168351872972437 1108 1 -0.008448109184122643 1108 2 0.03613357648481852 1108 3 0.04472941628563021 1108 4 -0.03335736898834796 1108 5 0.011905652439729408 1108 6 0.023044693613897493 1108 7 -12.17792183232655 1108 8 -6.726393986765533 1108 9 -2.64090082761966 1108 10-11 -0.5686987490432358 1108 12-13 -0.023347096405380796 1108 14-15 0.12037055199434121 1108 16-17 0.16481994473328143 1108 18-19 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10-11 -0.6832546440698906 2216 12-13 -1.0374596786548693 2216 14-15 -1.2972407238864179 2216 16-17 -1.4888306854804156 2216 18-19 -1.870959872546429 2216 20-21 -1.4746923531121965 2216 22-23 -1.2886503472703694 2216 24-25 -1.3403446147394078 2216 26-27 -1.7565175021183776 2216 28-29 -1.9338419438428218 2216 30-31 -1.8579900940258867 2216 32-33 -2.795427342202821 2216 34-35 -1.8866638812290475 2216 36-37 -1.5389800578341521 2216 38-39 -1.7843772725529377 2216 40-41 -1.7888785540502141 2216 42-43 -1.4236716180279245 2216 44-45 -1.5711413673017702 2216 46-47 -1.7959528932776578 2216 48-49 -1.8512793774874368 2216 50-51 -2.353399484875858 2216 52-53 -2.0221534584376073 2216 54-55 -1.6886102406275185 2216 56-57 -1.7408365266685095 2216 58-59 -1.980777929875977 2216 60-61 -2.133602242401196 2216 62-63 -2.0687511344058365 2216 64-65 -1.7904678013262014 2216 66-67 1.592345670975842 2216 68-69 0.846190281688969 2216 70-71 0.47318632768573465 2216 72-73 -1.260948091336612 2216 74-75 -2.0921898021939747 2216 76-77 -1.75788126155512 2216 78-79 0.8427922632795877 2216 80-81 -0.6569057384218802 2216 82-83 -1.548588432442294 2216 84-85 0.26551520550228247 2216 86-87 0.23292919259076328 2216 88-89 -1.0387682961777571 2216 90-91 -1.3294442728379323 2216 92-93 -1.4309372187369007 2216 94-95 -1.4253763413035543 2216 96-97 -1.4169239073468027 2216 98-99 -1.4570790989063056 2216 100 -2.57577212110181 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 136068.0 3 27488.0 4 16086.0 5 14096.0 6 13214.0 7 14294.0 8 15422.0 9 17463.0 10 19324.0 11 21590.0 12 23531.0 13 26657.0 14 29671.0 15 32904.0 16 35628.0 17 37036.0 18 35116.0 19 33161.0 20 32120.0 21 32903.0 22 35367.0 23 40690.0 24 49089.0 25 61251.0 26 77933.0 27 95546.0 28 118543.0 29 150567.0 30 186165.0 31 227657.0 32 301052.0 33 425441.0 34 640349.0 35 871190.0 36 998165.0 37 1644146.0 38 1778343.0 39 549830.0 40 1241.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41013438794212 19.93415689110607 14.441421596580328 23.214287124371484 2 15.51322065280357 20.006783118854138 40.18282840040207 24.297167827940225 3 18.800716336353258 24.758358291340645 29.955834647120433 26.485090725185668 4 13.0711726950327 16.650566135287097 37.65013675018754 32.62812441949266 5 13.66790481635454 37.68936973695682 34.8466031677428 13.796122278945841 6 31.588745306502712 36.849669354465775 17.568100121926598 13.993485217104915 7 27.96456310419596 32.39907228556424 21.754536228228158 17.881828382011644 8 25.376325167050425 36.34102478645348 19.99319899372176 18.289451052774343 9 25.24408896255579 17.364690739817355 21.15581665799529 36.23540363963156 10-11 24.37262423027683 27.030001228241158 28.169975943842424 20.42739859763959 12-13 24.549044323490072 24.470866604777147 28.3867170850826 22.59337198665018 14-15 22.01749719190687 26.63471397489177 25.53888375774573 25.808905075455623 16-17 21.812327891328405 29.927073942095074 26.333769422328423 21.926828744248102 18-19 21.8545832399558 29.0428617815903 27.92644809237456 21.176106886079335 20-21 22.810238478992986 28.07823163490355 27.64725881991959 21.464271066183873 22-23 22.62939843470854 27.861655592569466 27.659102925943706 21.849843046778286 24-25 22.373484111871676 28.015137480111573 27.595369993267795 22.016008414748956 26-27 22.079681545407965 28.1333155281239 27.626803186305388 22.160199740162746 28-29 22.073506793166107 28.157828875667594 27.83528868798919 21.933375643177108 30-31 22.331179286958037 28.27889512201573 27.47255870561646 21.917366885409777 32-33 22.156443445910853 28.15596861611929 27.709380482465416 21.978207455504446 34-35 22.346141693438824 27.985012741741045 27.67199715011784 21.996848414702292 36-37 22.43715821910396 28.03503522227816 27.481976909398124 22.045829649219755 38-39 22.256507975535545 28.182289576891005 27.612967368413038 21.94823507916041 40-41 22.217784087035273 28.012922812031775 27.67050968570841 22.098783415224542 42-43 22.18051388105025 28.090844105544434 27.620745534906654 22.107896478498663 44-45 22.217949498777394 28.10876440281876 27.56520743668173 22.108078661722114 46-47 22.23292636656514 28.08037992186996 27.54608941508941 22.140604296475487 48-49 22.230295260991525 28.034441072177497 27.506716603461808 22.228547063369174 50-51 22.158855563135443 28.077863825827226 27.51103772210718 22.25224288893016 52-53 22.209596403194272 28.117887579293825 27.513955493217217 22.158560524294685 54-55 22.18952288053818 28.20478753175718 27.476208653630827 22.129480934073808 56-57 22.149977696156803 28.07903286475525 27.50574232322019 22.265247115867762 58-59 22.16749254621852 27.867025779059034 27.627269758935658 22.33821191578679 60-61 22.249971112268188 28.048918927743788 27.45918060576093 22.241929354227093 62-63 22.077481615708503 28.165499283847552 27.480955894054777 22.276063206389168 64-65 22.190676232235433 28.018459676950012 27.466546149643523 22.324317941171028 66-67 22.040850943817233 27.98702879765413 27.498989673602324 22.473130584926313 68-69 22.34804647883427 28.094484430888066 27.360959916346495 22.19650917393117 70-71 22.143374846634487 27.936733891460864 27.47977853557279 22.440112726331858 72-73 22.090829467861038 28.01725788424669 27.555831785368007 22.33608086252427 74-75 22.079240914642995 28.08656876461793 27.46889154609469 22.36529877464439 76-77 22.16873946652897 27.991759557471834 27.328547068471988 22.510953907527206 78-79 22.212070173631034 27.96871698298606 27.512149068642955 22.307063774739955 80-81 22.068622590415686 28.003522045579167 27.545772296132682 22.382083067872465 82-83 22.090866635005977 27.956548267299024 27.628910305442204 22.323674792252795 84-85 22.17923868726437 27.931110414282124 27.444698110679017 22.444952787774493 86-87 22.239071869541995 27.947934777766058 27.45818715214705 22.354806200544893 88-89 22.08478078446588 27.88123043121261 27.54297801979662 22.49101076452489 90-91 22.086855206733123 27.816844536782003 27.505047360596972 22.591252895887894 92-93 22.171851284056636 27.89744713598676 27.438818822867532 22.491882757089073 94-95 22.180634201617437 27.732643411701268 27.570223984848973 22.516498401832315 96-97 22.18060837649099 27.764578535884034 27.50068392995542 22.554129157669557 98-99 22.15395577087407 27.77635533811153 27.513537364569956 22.55615152644444 100 22.18446156244311 27.651221291026555 27.51509116736685 22.649225979163486 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2796.0 1 2314.5 2 2242.5 3 2631.0 4 2505.5 5 2204.5 6 1943.0 7 1901.5 8 1979.0 9 2020.5 10 1900.5 11 1721.5 12 1718.0 13 1842.0 14 1930.5 15 2143.5 16 2493.0 17 3028.5 18 4033.0 19 5485.0 20 7690.5 21 11067.0 22 16117.5 23 23643.0 24 33237.0 25 44637.5 26 59170.0 27 76538.0 28 97207.0 29 119232.5 30 138610.0 31 157540.5 32 177337.0 33 196623.0 34 214980.5 35 233721.0 36 251371.0 37 268357.5 38 284723.0 39 296300.0 40 305043.5 41 310321.0 42 311717.5 43 314594.0 44 315104.0 45 312802.0 46 310356.5 47 303803.0 48 297427.0 49 295556.0 50 292036.0 51 282593.5 52 269365.5 53 258600.0 54 250394.0 55 236582.5 56 214890.5 57 191451.0 58 171589.0 59 154729.5 60 135569.5 61 114362.0 62 96584.0 63 80186.0 64 65003.5 65 52553.5 66 41676.5 67 32550.5 68 25260.5 69 19181.5 70 14094.0 71 10383.5 72 7750.5 73 5401.0 74 3663.0 75 2452.0 76 1541.5 77 1014.0 78 671.5 79 465.5 80 339.5 81 216.0 82 153.0 83 117.5 84 95.0 85 72.0 86 56.5 87 53.0 88 43.0 89 35.5 90 30.5 91 24.5 92 21.5 93 21.0 94 18.5 95 19.5 96 22.0 97 20.5 98 18.0 99 24.0 100 46.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.018361584947650875 2 0.0023572305000362608 3 0.0012519262464307413 4 0.001917364521560595 5 0.0016917922249064072 6 0.003913679346950156 7 11.5620577020702 8 2.2557229665418764E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.10161907899508E-4 18-19 0.0 20-21 5.639307416354691E-6 22-23 1.1278614832709382E-5 24-25 0.0 26-27 2.0301506698876885E-4 28-29 0.0 30-31 5.639307416354691E-6 32-33 2.2557229665418764E-5 34-35 6.20323815799016E-5 36-37 2.8196537081773453E-5 38-39 5.0753766747192214E-5 40-41 5.639307416354691E-6 42-43 5.6393074163546906E-5 44-45 1.9173645215605947E-4 46-47 0.0010489111794419726 48-49 7.049134270443364E-4 50-51 4.229480562266018E-4 52-53 2.8760467823408923E-4 54-55 4.0039082656118303E-4 56-57 0.001714349454571826 58-59 3.609156746467002E-4 60-61 0.001313958628010643 62-63 0.0023459518852035513 64-65 0.0010489111794419726 66-67 5.7814292418616615 68-69 5.4280927963825425 70-71 0.0029662757010025676 72-73 0.0014493020060031556 74-75 0.0033666665275637504 76-77 0.00621451677282287 78-79 5.419475934650352 80-81 0.005261473819458927 82-83 0.005114851826633704 84-85 5.781660453465732 86-87 0.0038572862727866085 88-89 0.0019963148253895604 90-91 0.003575320901968874 92-93 0.0037670573541249332 94-95 0.004539642470165526 96-97 0.0033553879127310405 98-99 0.005695700490518238 100 0.006259631232153706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8866337.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.06395673310881 #Duplication Level Percentage of deduplicated Percentage of total 1 87.62109606142225 71.02912740030705 2 8.285046059589463 13.43237230612748 3 2.0470152442323712 4.978174655714014 4 0.826087040038879 2.678635362859745 5 0.409259243207043 1.6588086791980292 6 0.2506970068431134 1.2193494789510018 7 0.15065067955092187 0.854863811825056 8 0.10624775700789639 0.6890290861662377 9 0.06614824530964984 0.48260146461772707 >10 0.23539838467336072 2.834307489702238 >50 0.0019857028887382247 0.10310359732721115 >100 3.685752363366995E-4 0.03962666720422882 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.1278614832709382E-5 0.0 0.0 0.0 4 0.0 1.1278614832709382E-5 0.0 0.0 0.0 5 0.0 1.1278614832709382E-5 0.0 1.1278614832709382E-5 0.0 6 0.0 2.2557229665418764E-5 0.0 1.1278614832709382E-5 0.0 7 0.0 2.2557229665418764E-5 0.0 1.1278614832709382E-5 0.0 8 0.0 2.2557229665418764E-5 0.0 2.2557229665418764E-5 0.0 9 0.0 2.2557229665418764E-5 0.0 4.511445933083753E-5 0.0 10-11 0.0 2.2557229665418764E-5 0.0 8.458961124532035E-5 0.0 12-13 0.0 2.2557229665418764E-5 0.0 1.0150753349438443E-4 0.0 14-15 0.0 2.2557229665418764E-5 0.0 1.0150753349438443E-4 0.0 16-17 0.0 3.383584449812814E-5 0.0 1.5790060765793134E-4 0.0 18-19 0.0 3.383584449812814E-5 0.0 1.6353991507428602E-4 0.0 20-21 0.0 4.511445933083753E-5 0.0 2.1429368182147824E-4 0.0 22-23 0.0 7.895030382896567E-5 0.0 3.8911221172847367E-4 0.0 24-25 0.0 1.0150753349438444E-4 0.0 6.372417380480801E-4 1.1278614832709382E-5 26-27 0.0 1.1278614832709381E-4 0.0 0.0010827470239401006 1.1278614832709382E-5 28-29 0.0 1.1278614832709381E-4 0.0 0.003265158994069366 1.1278614832709382E-5 30-31 0.0 1.1278614832709381E-4 0.0 0.009603740530052038 1.1278614832709382E-5 32-33 0.0 1.2406476315980318E-4 0.0 0.02364561599677522 1.1278614832709382E-5 34-35 0.0 1.2406476315980318E-4 0.0 0.04640586072918275 1.1278614832709382E-5 36-37 0.0 1.4098268540886725E-4 0.0 0.08349558560654755 1.1278614832709382E-5 38-39 0.0 1.7481852990699543E-4 0.0 0.1323150699099301 1.1278614832709382E-5 40-41 0.0 1.804578373233501E-4 0.0 0.18218346539275465 1.1278614832709382E-5 42-43 0.0 1.860971447397048E-4 0.0 0.23248044823922212 1.1278614832709382E-5 44-45 0.0 2.0301506698876885E-4 0.0 0.2957140022988073 1.1278614832709382E-5 46-47 0.0 2.0301506698876885E-4 0.0 0.37283717052487403 1.1278614832709382E-5 48-49 0.0 2.0301506698876885E-4 0.0 0.45244163401413684 1.1278614832709382E-5 50-51 0.0 2.2557229665418763E-4 0.0 0.5329314687677673 1.1278614832709382E-5 52-53 0.0 2.2557229665418763E-4 0.0 0.6117295112964914 1.1278614832709382E-5 54-55 0.0 2.2557229665418763E-4 0.0 0.698230847755956 1.1278614832709382E-5 56-57 0.0 2.2557229665418763E-4 0.0 0.7940314021449895 1.1278614832709382E-5 58-59 0.0 2.2557229665418763E-4 0.0 0.899610515593982 1.1278614832709382E-5 60-61 0.0 2.2557229665418763E-4 0.0 1.00804311859565 1.1278614832709382E-5 62-63 0.0 2.2557229665418763E-4 0.0 1.117603583080589 1.1278614832709382E-5 64-65 0.0 2.2557229665418763E-4 0.0 1.2338297089316592 1.1278614832709382E-5 66-67 0.0 2.2557229665418763E-4 0.0 1.3643740363128538 1.1278614832709382E-5 68-69 0.0 2.2557229665418763E-4 0.0 1.496040585869903 1.1278614832709382E-5 70-71 0.0 2.2557229665418763E-4 0.0 1.6535013275493589 1.1278614832709382E-5 72-73 0.0 2.2557229665418763E-4 0.0 1.8192180152863577 1.1278614832709382E-5 74-75 0.0 2.36850911486897E-4 1.1278614832709382E-5 1.9700187349070988 1.1278614832709382E-5 76-77 1.1278614832709382E-5 2.4812952631960637E-4 1.1278614832709382E-5 2.125714373365235 1.1278614832709382E-5 78-79 1.1278614832709382E-5 2.4812952631960637E-4 1.1278614832709382E-5 2.3133792455666864 1.1278614832709382E-5 80-81 1.1278614832709382E-5 2.4812952631960637E-4 1.1278614832709382E-5 2.5366168689505035 1.1278614832709382E-5 82-83 1.1278614832709382E-5 2.4812952631960637E-4 1.1278614832709382E-5 2.7655445535174223 1.1278614832709382E-5 84-85 1.1278614832709382E-5 2.594081411523158E-4 1.1278614832709382E-5 2.993045493308003 1.1278614832709382E-5 86-87 1.1278614832709382E-5 2.763260634013799E-4 1.1278614832709382E-5 3.263732249293028 1.1278614832709382E-5 88 1.1278614832709382E-5 2.8196537081773455E-4 1.1278614832709382E-5 3.490325260589576 1.1278614832709382E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9965 0.0 41.79732 1 GTATCAA 15360 0.0 34.77848 1 TCAACGC 16350 0.0 31.66491 4 ATCAACG 16700 0.0 31.031906 3 CAACGCA 17740 0.0 29.154997 5 TATCAAC 18560 0.0 28.335485 2 AACGCAG 19085 0.0 27.180391 6 GTACATG 29365 0.0 24.743416 1 TACATGG 29850 0.0 23.051193 2 ACGCAGA 22355 0.0 22.700958 7 CGCAGAG 23175 0.0 21.708487 8 ACATGGG 30715 0.0 21.635857 3 GTGGTAT 5700 0.0 20.19803 1 CATGGGG 22530 0.0 18.5968 4 GCAGAGT 26640 0.0 17.95086 9 GAGTACA 22715 0.0 17.232569 1 AGAGTAC 22515 0.0 16.10566 10-11 CAGAGTA 25050 0.0 15.992921 10-11 AGTACAT 23535 0.0 15.281169 2 TGGTATC 6840 0.0 14.958547 2 >>END_MODULE