FastQCFastQC Report
Wed 25 May 2016
SRR2049520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049520_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8866337
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286120.3227037275934808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283180.31938781483266426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171120.19299965701732294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169050.055.0314181
GTATCAA291400.039.4382061
ATCAACG375900.030.265183
TCAACGC377300.030.140414
TATCAAC385750.029.6021442
GTGGTAT66150.029.1093431
CAACGCA391300.029.1024765
AACGCAG411400.027.6915556
ACGCAGA474950.023.8275997
CGCAGAG478100.023.5526478
GTACATG294500.022.98861
TGGTATC82350.022.8536592
TACATGG297600.022.26022
GCAGAGT518300.021.3813089
ACATGGG306650.020.7440263
GAGTACT338100.020.69070812-13
AGAGTAC476500.019.962710-11
CAGAGTA502400.019.6211310-11
AGTACTT350050.019.29293612-13
GTACTTT363650.019.06251114-15
Produced by FastQC (version 0.11.5)