FastQCFastQC Report
Wed 25 May 2016
SRR2049519_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049519_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8592426
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89200.042.8099861
GTATCAA141250.037.58041
TCAACGC148200.034.4657484
ATCAACG149250.034.2918553
CAACGCA163250.031.2877355
TATCAAC172900.030.134092
AACGCAG177500.028.6878916
ACGCAGA203550.024.5626077
GTACATG237050.023.7532941
CGCAGAG205750.023.6574638
TACATGG239800.022.5169012
ACATGGG248550.020.9417293
GTGGTAT53650.020.6089131
GCAGAGT238150.019.261199
CATGGGG175150.018.4384564
GTCGTAC3658.4946805E-1018.2314661
GAGTACA190050.017.1839871
CAGAGTA223550.016.92737410-11
AGAGTAC204450.016.7828910-11
TGGTATC62000.016.179672
Produced by FastQC (version 0.11.5)