##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049519_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8592426 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.734346271937635 31.0 31.0 34.0 28.0 34.0 2 30.98366840750214 31.0 31.0 34.0 28.0 34.0 3 31.074862442807188 31.0 31.0 34.0 27.0 34.0 4 34.644565108852845 37.0 35.0 37.0 32.0 37.0 5 34.62975252856411 37.0 35.0 37.0 32.0 37.0 6 34.68159504661431 37.0 35.0 37.0 32.0 37.0 7 30.740523456355632 37.0 33.0 37.0 2.0 37.0 8 32.530398399706904 37.0 33.0 37.0 17.0 37.0 9 35.30168581027058 39.0 34.0 39.0 28.0 39.0 10-11 35.8450054152343 39.0 36.0 39.0 30.0 39.0 12-13 35.99057390776481 39.0 37.0 39.0 31.0 39.0 14-15 37.18314740214231 40.0 37.0 41.0 31.0 41.0 16-17 37.1968598856714 40.0 37.0 41.0 31.0 41.0 18-19 37.19943017257292 40.0 37.5 41.0 31.0 41.0 20-21 37.26658384954378 40.0 38.0 41.0 31.0 41.0 22-23 37.22820190712146 40.0 37.0 41.0 31.0 41.0 24-25 37.206509605087085 40.0 37.0 41.0 31.0 41.0 26-27 37.23262440665768 40.0 37.0 41.0 31.0 41.0 28-29 37.22085898674018 40.0 37.0 41.0 31.0 41.0 30-31 37.148187135973004 40.0 37.0 41.0 31.0 41.0 32-33 37.10198336302227 40.0 37.0 41.0 30.0 41.0 34-35 37.04688972590512 40.0 37.0 41.0 30.0 41.0 36-37 36.8793847628132 40.0 37.0 41.0 30.0 41.0 38-39 36.77417128759677 40.0 37.0 41.0 30.0 41.0 40-41 36.702931279245234 40.0 37.0 41.0 30.0 41.0 42-43 36.59125746325892 40.0 36.0 41.0 30.0 41.0 44-45 36.47941169350774 40.0 36.0 41.0 30.0 41.0 46-47 36.330125217255286 40.0 36.0 41.0 29.5 41.0 48-49 36.253538465155245 40.0 36.0 41.0 29.0 41.0 50-51 36.123581745132284 40.0 35.0 41.0 29.0 41.0 52-53 36.012005282326555 39.0 35.0 41.0 29.0 41.0 54-55 35.89976445534707 39.0 35.0 41.0 28.5 41.0 56-57 35.70724519477969 39.0 35.0 41.0 28.0 41.0 58-59 35.53595817991334 39.0 35.0 41.0 28.0 41.0 60-61 35.326176390695714 39.0 35.0 40.5 28.0 41.0 62-63 35.10192924559374 38.0 34.0 40.0 28.0 41.0 64-65 34.75200775659866 38.0 34.0 40.0 26.5 41.0 66-67 31.260743124235226 36.5 30.5 40.0 6.5 41.0 68-69 31.627893856752447 36.0 30.5 39.5 11.0 41.0 70-71 31.792027304046613 36.0 30.0 39.0 15.5 41.0 72-73 33.0346810085999 35.5 32.5 39.0 24.0 41.0 74-75 33.112658636804085 35.0 33.0 39.0 25.0 40.0 76-77 32.81097777275009 35.0 33.0 37.5 24.5 40.0 78-79 29.430343362863994 35.0 29.0 37.0 6.5 39.0 80-81 31.213729161007613 35.0 30.0 36.0 20.5 39.0 82-83 31.613233270789877 35.0 32.0 36.0 22.5 38.5 84-85 29.773282306999214 35.0 30.5 36.0 11.5 37.0 86-87 29.40615240678244 35.0 29.0 35.0 13.5 37.0 88-89 30.640674473076636 35.0 30.5 35.0 20.0 36.5 90-91 30.788329337954146 35.0 32.0 35.0 18.5 36.0 92-93 30.641616116333154 35.0 32.0 35.0 17.5 36.0 94-95 30.43814232441455 35.0 32.0 35.0 9.5 36.0 96-97 30.2543872359215 35.0 32.0 35.0 2.0 35.5 98-99 30.075797277742048 35.0 32.0 35.0 2.0 35.0 100 29.875481965163274 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38125728781422197 1101 2 0.4287664653332115 1101 3 0.4146278075348562 1101 4 0.46905352941132605 1101 5 0.40153282759428777 1101 6 0.38903391183924896 1101 7 -12.133895182147484 1101 8 -6.501756468689845 1101 9 -2.1789986807755923 1101 10-11 -0.09327838020080748 1101 12-13 0.44151540659635913 1101 14-15 0.6279470978196571 1101 16-17 0.7217618222769246 1101 18-19 0.6788043189892363 1101 20-21 0.6125376041579642 1101 22-23 0.6317974648379305 1101 24-25 0.6837267275760794 1101 26-27 0.6457368395377685 1101 28-29 0.5981493841903855 1101 30-31 0.634212935952533 1101 32-33 0.6379594181923167 1101 34-35 0.6151704167240695 1101 36-37 0.6926647306302343 1101 38-39 0.6801129380808533 1101 40-41 0.7144093207693061 1101 42-43 0.686729531960367 1101 44-45 0.5637568024829989 1101 46-47 0.5853493010911208 1101 48-49 0.49732533581148886 1101 50-51 0.6070976140419972 1101 52-53 0.6401820418153363 1101 54-55 0.5711728851613103 1101 56-57 0.5347057265845692 1101 58-59 0.5240651649116685 1101 60-61 0.4487232020621761 1101 62-63 0.49222798131035006 1101 64-65 0.3543973254230224 1101 66-67 -10.314146985150725 1101 68-69 -8.217440647059625 1101 70-71 -6.734683609954935 1101 72-73 -1.5552802473518383 1101 74-75 -0.25475687292772164 1101 76-77 0.032923107769804005 1101 78-79 3.156631894957272 1101 80-81 1.0341626285955599 1101 82-83 0.3564067101877093 1101 84-85 -5.398867591756549 1101 86-87 -5.947882611692293 1101 88-89 -1.359057814136726 1101 90-91 -0.07935524189018395 1101 92-93 0.2328332958035837 1101 94-95 0.32704291409117303 1101 96-97 0.4293120152609937 1101 98-99 0.44855558315010313 1101 100 0.48947778677920084 1102 1 0.161585851287672 1102 2 0.22084003762035564 1102 3 0.20635485611657955 1102 4 0.21063934860135447 1102 5 0.18750420244507637 1102 6 0.18346775102363466 1102 7 -12.186863511055648 1102 8 -6.620644641435337 1102 9 -2.395797562457439 1102 10-11 -0.2817799757318298 1102 12-13 0.22616497616952103 1102 14-15 0.3802815161680897 1102 16-17 0.4030809007793934 1102 18-19 0.3875415436970826 1102 20-21 0.3411045490405584 1102 22-23 0.3313016049280151 1102 24-25 0.37657233935014034 1102 26-27 0.3327855220037179 1102 28-29 0.36021845450776624 1102 30-31 0.33146078531584067 1102 32-33 0.3639729178394191 1102 34-35 0.37637583580511347 1102 36-37 0.5189007047720224 1102 38-39 0.5074283503436021 1102 40-41 0.4138394581147793 1102 42-43 0.4307494948677899 1102 44-45 0.42648500228659714 1102 46-47 0.46966540566614867 1102 48-49 0.3807921581525662 1102 50-51 0.4425755964216833 1102 52-53 0.4198321787292656 1102 54-55 0.41595209771411845 1102 56-57 0.446927107646502 1102 58-59 0.413035897139288 1102 60-61 0.3509565268893695 1102 62-63 0.3418474716130504 1102 64-65 0.24528902514258988 1102 66-67 -10.282099967950735 1102 68-69 -6.7158246379897975 1102 70-71 -6.262741200589748 1102 72-73 -1.1022099774848222 1102 74-75 -0.20384550312616767 1102 76-77 -0.06478783051243653 1102 78-79 3.0917637314049387 1102 80-81 0.9794673001141021 1102 82-83 0.32879416723980626 1102 84-85 -5.48335464049347 1102 86-87 -5.953513296884058 1102 88-89 -1.4475567686573676 1102 90-91 -0.22596455892558254 1102 92-93 0.11966124970052405 1102 94-95 0.16398119823897872 1102 96-97 0.25568184030154484 1102 98-99 0.234336080336643 1102 100 0.31584475639743914 1103 1 0.1286964935058066 1103 2 0.14111468783646686 1103 3 0.16730919307153158 1103 4 0.16454243970760984 1103 5 0.16534546860138732 1103 6 0.21899852400076725 1103 7 -12.123193512141889 1103 8 -6.628198014436588 1103 9 -2.35584274381268 1103 10-11 -0.3553799593871716 1103 12-13 0.1758387165349049 1103 14-15 0.3593469983535087 1103 16-17 0.36949216299669985 1103 18-19 0.381320092794148 1103 20-21 0.35843012923784556 1103 22-23 0.36840913800232045 1103 24-25 0.48731294308743145 1103 26-27 0.4028111845953717 1103 28-29 0.3546011612348323 1103 30-31 0.3733826098061428 1103 32-33 0.3801179619345021 1103 34-35 0.39558751537627046 1103 36-37 0.4632971015861429 1103 38-39 0.48269331765521883 1103 40-41 0.42988456428144417 1103 42-43 0.399797128033093 1103 44-45 0.38987080635722293 1103 46-47 0.39674636610149605 1103 48-49 0.34230461015028624 1103 50-51 0.37719478409911034 1103 52-53 0.35088515519085206 1103 54-55 0.35243610455067653 1103 56-57 0.3543964350271125 1103 58-59 0.3166311580123633 1103 60-61 0.32882190129322453 1103 62-63 0.2426410046042804 1103 64-65 0.11425567828785432 1103 66-67 -10.344037609638352 1103 68-69 -8.26030031605167 1103 70-71 -6.767074420517556 1103 72-73 -1.6868273749426095 1103 74-75 -0.5022743398654086 1103 76-77 -0.17721105845226504 1103 78-79 2.995143205889015 1103 80-81 0.8889171356511483 1103 82-83 0.1656506198151888 1103 84-85 -5.568260962671154 1103 86-87 -6.057389327719104 1103 88-89 -1.5630367160594716 1103 90-91 -0.30745036076566734 1103 92-93 0.06431900035533644 1103 94-95 0.14029860055291365 1103 96-97 0.21850348599573977 1103 98-99 0.2352352776159634 1103 100 0.336437853522753 1104 1 0.11877644327697467 1104 2 0.18107616043786479 1104 3 0.18634317390130306 1104 4 0.22760978898086393 1104 5 0.13961070998777103 1104 6 0.21273571361567178 1104 7 -12.311417364484559 1104 8 -6.687322798840594 1104 9 -2.3259606358481264 1104 10-11 -0.29934752527915265 1104 12-13 0.256331989150425 1104 14-15 0.4273081128577161 1104 16-17 0.44107668730912053 1104 18-19 0.45669234691509786 1104 20-21 0.43771697266812026 1104 22-23 0.4286936410832567 1104 24-25 0.5180049327051179 1104 26-27 0.49169383302606207 1104 28-29 0.4986644442004575 1104 30-31 0.47377074368311867 1104 32-33 0.4597377461652812 1104 34-35 0.48217748663766 1104 36-37 0.5082840572882859 1104 38-39 0.5141963291235356 1104 40-41 0.5342332837864063 1104 42-43 0.4411166980132535 1104 44-45 0.43099069142029833 1104 46-47 0.43937071983035025 1104 48-49 0.38473032831965526 1104 50-51 0.42838073358321793 1104 52-53 0.41679347566929437 1104 54-55 0.4203005622580278 1104 56-57 0.4064360635307196 1104 58-59 0.38911515092384974 1104 60-61 0.34657326957719903 1104 62-63 0.24903052100204093 1104 64-65 0.18602989825964045 1104 66-67 -10.497891752818465 1104 68-69 -8.38460580891551 1104 70-71 -6.83650779310835 1104 72-73 -1.6689419332160114 1104 74-75 -0.4638319924794345 1104 76-77 -0.17536905373949452 1104 78-79 3.0046317665444207 1104 80-81 0.9338742238172912 1104 82-83 0.22394469516733295 1104 84-85 -5.608255062462369 1104 86-87 -6.077064497356375 1104 88-89 -1.495231157070446 1104 90-91 -0.19314946816017908 1104 92-93 0.11069498599185934 1104 94-95 0.1560246460529342 1104 96-97 0.2883634177455221 1104 98-99 0.33318610465695997 1104 100 0.4393041346063704 1105 1 0.14985507988660984 1105 2 0.17724412598512984 1105 3 0.1537255131734483 1105 4 0.2416613359886668 1105 5 0.22978676469873704 1105 6 0.2761790612849566 1105 7 -12.193727508285193 1105 8 -6.645119662995334 1105 9 -2.3953293515712133 1105 10-11 -0.30364633902492244 1105 12-13 0.21970201395549083 1105 14-15 0.3800475803848187 1105 16-17 0.4410911486609592 1105 18-19 0.42391618590019675 1105 20-21 0.43491327883604214 1105 22-23 0.42813174710220636 1105 24-25 0.4755878039324344 1105 26-27 0.4661293080587825 1105 28-29 0.4341315174381748 1105 30-31 0.437286537221226 1105 32-33 0.4114214745255751 1105 34-35 0.45531516572314246 1105 36-37 0.5292432313981195 1105 38-39 0.506004593250843 1105 40-41 0.502137840557765 1105 42-43 0.4543376698886732 1105 44-45 0.4250584646575959 1105 46-47 0.4256072809341873 1105 48-49 0.40670241397923945 1105 50-51 0.47021182378407644 1105 52-53 0.42382775905275594 1105 54-55 0.40657755587422884 1105 56-57 0.42173428672592905 1105 58-59 0.3889705192607096 1105 60-61 0.3085951105127265 1105 62-63 0.2895705529996775 1105 64-65 0.1978432804111776 1105 66-67 -10.396532258732318 1105 68-69 -8.331197054783651 1105 70-71 -6.741381831165352 1105 72-73 -1.6022990583258725 1105 74-75 -0.379758664534684 1105 76-77 -0.10964769538126262 1105 78-79 3.010825192780395 1105 80-81 0.8957814565738076 1105 82-83 0.2095899012927056 1105 84-85 -5.498118719313055 1105 86-87 -6.016398709056048 1105 88-89 -1.479674837151233 1105 90-91 -0.15997386900296817 1105 92-93 0.229502878273113 1105 94-95 0.26815462354620934 1105 96-97 0.34343044317790117 1105 98-99 0.4104473133235622 1105 100 0.4815847242666287 1106 1 0.07228630249872836 1106 2 0.1335654389307095 1106 3 0.08850712446480458 1106 4 0.09398154493456445 1106 5 0.06532267203810704 1106 6 0.10406902047059674 1106 7 -12.281268310838893 1106 8 -6.762480640381003 1106 9 -2.539228496834589 1106 10-11 -0.4766414162141288 1106 12-13 0.08626131098159107 1106 14-15 0.17734373589606633 1106 16-17 0.24974470437439322 1106 18-19 0.28095371176659967 1106 20-21 0.2516924210771947 1106 22-23 0.26024724825960277 1106 24-25 0.3268954132935491 1106 26-27 0.32970112584946776 1106 28-29 0.2321814969216618 1106 30-31 0.19892287020148558 1106 32-33 0.26292194797529334 1106 34-35 0.31753066990793144 1106 36-37 0.33843822689707537 1106 38-39 0.27963960965641377 1106 40-41 0.286206826469261 1106 42-43 0.2947111413584267 1106 44-45 0.19964006637121656 1106 46-47 0.28674881568445443 1106 48-49 0.26674235933963786 1106 50-51 0.27674523085986635 1106 52-53 0.2545832621820665 1106 54-55 0.2099065695968605 1106 56-57 0.23492289642545217 1106 58-59 0.19997760166547351 1106 60-61 0.1917655941468368 1106 62-63 0.1456902643448359 1106 64-65 0.011931123988098591 1106 66-67 -10.509677479149541 1106 68-69 -8.437188333923672 1106 70-71 -6.945571416699504 1106 72-73 -1.8450120785818456 1106 74-75 -0.6169678831832925 1106 76-77 -0.35937868265305184 1106 78-79 2.7993465812534026 1106 80-81 0.6670643730842016 1106 82-83 0.02514099185701113 1106 84-85 -5.726961108386583 1106 86-87 -6.1891062075521575 1106 88-89 -1.6580996164928017 1106 90-91 -0.36951142710439555 1106 92-93 -0.028381758786700573 1106 94-95 0.01786445841809936 1106 96-97 0.09076721951716848 1106 98-99 0.13117430307653422 1106 100 0.21840781546995203 1107 1 0.1583808476560833 1107 2 0.1387752287603199 1107 3 0.16531597893686012 1107 4 0.1384797100611337 1107 5 0.14540486600178326 1107 6 0.11594044571683781 1107 7 -12.228763160430514 1107 8 -6.711902392849954 1107 9 -2.5049818407829036 1107 10-11 -0.4743341227035174 1107 12-13 0.12723448023310624 1107 14-15 0.25151334906100686 1107 16-17 0.2877897931848068 1107 18-19 0.33874613550834454 1107 20-21 0.2920773944915851 1107 22-23 0.33755661327899134 1107 24-25 0.3988343146205935 1107 26-27 0.2963312970917116 1107 28-29 0.3437169494168728 1107 30-31 0.3278647374830328 1107 32-33 0.3492405711240423 1107 34-35 0.30893673769698893 1107 36-37 0.38216672615649117 1107 38-39 0.373524120283534 1107 40-41 0.3503545646266133 1107 42-43 0.3646781563611725 1107 44-45 0.27309949753521323 1107 46-47 0.3353972158739964 1107 48-49 0.2724113319570307 1107 50-51 0.2969327759880471 1107 52-53 0.2917195556367318 1107 54-55 0.24614012731770885 1107 56-57 0.2280713928548792 1107 58-59 0.22159329726748922 1107 60-61 0.19631340584125923 1107 62-63 0.19871690923077523 1107 64-65 0.052799971015979 1107 66-67 -10.406213320740758 1107 68-69 -8.379207746118837 1107 70-71 -6.886309554552877 1107 72-73 -1.8304800753315433 1107 74-75 -0.552448456920132 1107 76-77 -0.27664601574498704 1107 78-79 2.826466826041184 1107 80-81 0.7805136191901347 1107 82-83 0.10275316443465599 1107 84-85 -5.65898942162584 1107 86-87 -6.093368684451697 1107 88-89 -1.5902136207519142 1107 90-91 -0.3269298578080857 1107 92-93 0.06883818112017437 1107 94-95 0.1291900346274062 1107 96-97 0.1795660942869084 1107 98-99 0.22145416367154525 1107 100 0.3632851769437373 1108 1 0.018951527813626967 1108 2 0.08871451515680207 1108 3 0.07560395531526254 1108 4 -0.02619473963903829 1108 5 -0.016170756496961758 1108 6 0.007093847868155478 1108 7 -12.322614545856965 1108 8 -6.792360389490611 1108 9 -2.6638079014361935 1108 10-11 -0.5909825405523534 1108 12-13 -0.02189076273494095 1108 14-15 0.1575425568884441 1108 16-17 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-0.057939476839109716 2216 10-11 -0.7305045402864252 2216 12-13 -1.14432361071389 2216 14-15 -1.343099099332285 2216 16-17 -1.5222811428641663 2216 18-19 -1.9844163045779908 2216 20-21 -1.5370099119084912 2216 22-23 -1.3858377362793206 2216 24-25 -1.4509928125755138 2216 26-27 -1.8047946935213162 2216 28-29 -1.961088633203687 2216 30-31 -1.8593176440002992 2216 32-33 -2.7528058734748626 2216 34-35 -1.842424428338198 2216 36-37 -1.5747011322243907 2216 38-39 -1.7898737737436292 2216 40-41 -1.7869967041858459 2216 42-43 -1.415750959674348 2216 44-45 -1.620084622712767 2216 46-47 -1.760257630256973 2216 48-49 -1.8032530252950139 2216 50-51 -2.3589904379039908 2216 52-53 -2.0658031381794757 2216 54-55 -1.7179489624445878 2216 56-57 -1.7744446852244735 2216 58-59 -2.0805291284793554 2216 60-61 -2.187573443926482 2216 62-63 -2.0416383282675454 2216 64-65 -1.7448147979629383 2216 66-67 1.6153595577328979 2216 68-69 0.8210933349463261 2216 70-71 0.4234418925936474 2216 72-73 -1.428948851389329 2216 74-75 -2.206782223718413 2216 76-77 -1.8600451974618721 2216 78-79 0.7806470386224582 2216 80-81 -0.6742612329243336 2216 82-83 -1.5847976600919012 2216 84-85 0.21072741779262572 2216 86-87 0.2255532952936221 2216 88-89 -1.059734046164234 2216 90-91 -1.354861667145137 2216 92-93 -1.4303351677799583 2216 94-95 -1.4165020614857795 2216 96-97 -1.3854690239146663 2216 98-99 -1.425335863216418 2216 100 -2.496124499806278 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 130032.0 3 25897.0 4 14923.0 5 13125.0 6 11687.0 7 12640.0 8 14096.0 9 15873.0 10 18060.0 11 20413.0 12 22805.0 13 26227.0 14 29893.0 15 32584.0 16 35163.0 17 36955.0 18 34572.0 19 32165.0 20 31070.0 21 31244.0 22 33715.0 23 38495.0 24 46369.0 25 58702.0 26 73390.0 27 89331.0 28 110189.0 29 138889.0 30 170910.0 31 209116.0 32 275961.0 33 386213.0 34 587050.0 35 830133.0 36 888287.0 37 1530583.0 38 1853390.0 39 680241.0 40 2038.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.159070874312036 21.053208086890738 15.195658862103082 22.592062176694146 2 14.96812858850888 20.47562301687476 41.18765859619145 23.368589798424917 3 18.150129040329833 25.62379579331434 30.66900209781461 25.557073068541218 4 12.541752965828094 17.378707488788674 39.24934743169506 30.830192113688177 5 12.940732704190975 38.11734002341186 35.71072269281558 13.23120457958159 6 30.10866472106401 38.351552748042515 18.107582321978814 13.432200208914663 7 26.549213853783176 33.018812531366066 22.870819986023832 17.561153628826922 8 24.189684330019602 37.165779664246045 20.78093687892228 17.863599126812076 9 24.259528100678434 17.879513888161505 22.307262233041055 35.55369577811901 10-11 23.329936155400116 27.805249646607372 29.15738814625811 19.7074260517344 12-13 23.68497558198348 25.258355440011936 29.264488283053 21.792180694951576 14-15 21.16652503029994 27.648652429476844 26.441990888254374 24.74283165196884 16-17 20.617379383801893 30.948906120397808 27.317551245001543 21.11616325079876 18-19 20.619549123844653 29.911331211930136 29.18864823508518 20.280471429140036 20-21 21.690800712162083 29.024154528651163 28.777140472318298 20.50790428686846 22-23 21.455200365438163 28.83190135497255 28.792669124257703 20.920229155331587 24-25 21.260799918439798 28.985899907662866 28.741498617503368 21.01180155639397 26-27 20.992897681976388 29.035202712720974 28.80736780827163 21.164531797031007 28-29 20.955711460302364 29.017922295752097 28.999912248298678 21.02645399564686 30-31 21.277315700904246 29.277483988659547 28.59129823121539 20.85390207922082 32-33 21.004071668014074 29.08778540911241 28.88128374011317 21.026859182760347 34-35 21.213628706783723 28.910416643875266 28.833919004443683 21.042035644897332 36-37 21.345145366130364 29.09350556411639 28.522746984858582 21.038602084894663 38-39 21.182192798363907 29.191232158334856 28.66451806810012 20.962056975201115 40-41 21.15984260321403 28.950967215365115 28.72677554513281 21.162414636288045 42-43 21.113038843236584 28.95663010053335 28.726083941984932 21.20424711424514 44-45 21.228004501413835 29.03216402054301 28.62852297053045 21.111308507512703 46-47 21.17647839274161 29.117534133093958 28.5775054260659 21.12848204809853 48-49 21.157666522914823 29.0097468638831 28.552020118818433 21.280566494383642 50-51 21.1411024345082 29.009213673607203 28.495160108200363 21.35452378368423 52-53 21.11163198467297 29.030429555038328 28.551558859611216 21.306379600677484 54-55 21.236304522740735 29.157494547936867 28.462647090195343 21.143553839127055 56-57 21.155126483474106 29.001674001053384 28.435480256511404 21.4077192589611 58-59 21.10467758216754 28.781695821021337 28.716056407300012 21.39757018951111 60-61 21.239378146200284 29.018643300654105 28.437315278116742 21.304663275028865 62-63 21.09952589094683 29.098854120555433 28.459444393978906 21.342175594518835 64-65 21.19596394044417 28.933610758697775 28.473357842757867 21.397067458100185 66-67 21.097957742459243 28.928092154243064 28.42203723658841 21.551912866709284 68-69 21.5602458102319 29.052131896395956 28.266779627971143 21.120842665401003 70-71 21.301126936420058 28.903402922483608 28.34599839108842 21.449471750007916 72-73 21.16050866102657 29.024785493589366 28.48121640299599 21.33348944238808 74-75 21.200029422355822 29.074332881833627 28.34965606802337 21.37598162778718 76-77 21.247936274603944 28.986727561478215 28.25871914886931 21.506617015048537 78-79 21.36898828287049 29.017510941916253 28.37132250501823 21.242178270195026 80-81 21.203687797256578 28.993171944193048 28.383091803632816 21.42004845491756 82-83 21.175300956337704 29.008656248225083 28.517667247365676 21.298375548071544 84-85 21.27425333350136 28.89547645045578 28.34859888298084 21.48167133306202 86-87 21.347091173214668 28.96873288569842 28.290436975637878 21.393738965449035 88-89 21.21649919476737 28.903158228575503 28.329215760155606 21.551126816501522 90-91 21.16477361174372 28.86391274180961 28.37897108430692 21.592342562139752 92-93 21.28459214170986 28.979312175713446 28.25852247218048 21.477573210396212 94-95 21.226805595951674 28.78190017989997 28.392211961898962 21.599082262249393 96-97 21.265530067481574 28.879271164996123 28.318193303331434 21.537005464190866 98-99 21.276834714175845 28.851571767497035 28.266348970466915 21.605244547860206 100 21.266507452861163 28.787119254740652 28.275992425802226 21.670380866595956 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2377.0 1 2038.5 2 1932.0 3 2204.0 4 2154.5 5 1955.0 6 1780.5 7 1697.0 8 1785.5 9 1843.0 10 1715.5 11 1621.5 12 1628.0 13 1747.5 14 1994.5 15 2364.0 16 2853.0 17 3592.5 18 4890.5 19 6772.0 20 9693.5 21 14147.5 22 21143.0 23 31733.5 24 44566.0 25 59257.0 26 78646.5 27 102226.5 28 130465.0 29 158997.0 30 182020.0 31 204903.0 32 226304.5 33 244957.5 34 262991.5 35 279622.5 36 293146.0 37 303514.5 38 311844.5 39 315864.5 40 317859.5 41 316188.0 42 310226.0 43 305325.5 44 298894.0 45 291621.0 46 284530.5 47 276026.5 48 267176.5 49 260011.5 50 251461.5 51 237113.0 52 220267.5 53 206217.0 54 195964.5 55 184285.5 56 166451.0 57 147014.5 58 130578.0 59 116280.5 60 100404.0 61 82362.0 62 67622.0 63 54802.0 64 43227.5 65 34097.5 66 26427.0 67 20106.0 68 15290.0 69 11556.0 70 8579.0 71 6232.0 72 4448.0 73 3143.0 74 2161.0 75 1436.0 76 920.5 77 617.5 78 425.5 79 286.0 80 188.5 81 121.5 82 98.0 83 73.0 84 62.0 85 53.0 86 38.5 87 28.0 88 28.0 89 28.5 90 23.5 91 20.0 92 18.0 93 19.0 94 16.0 95 14.5 96 17.5 97 19.5 98 19.0 99 24.0 100 47.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.01958701768278249 2 0.0028280720718456 3 0.0017573616578135207 4 0.0025836707816861036 5 0.0023276313348523456 6 0.004666900826378953 7 11.59902919152286 8 3.491447002278518E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.142302302050667E-4 18-19 0.0 20-21 0.0 22-23 1.1638156674261727E-5 24-25 0.0 26-27 2.3276313348523454E-4 28-29 0.0 30-31 5.819078337130864E-6 32-33 1.745723501139259E-5 34-35 4.655262669704691E-5 36-37 1.745723501139259E-5 38-39 4.0733548359916046E-5 40-41 1.745723501139259E-5 42-43 4.0733548359916046E-5 44-45 1.6875327177679505E-4 46-47 0.0012220064507974815 48-49 8.437663588839752E-4 50-51 3.898782485877679E-4 52-53 2.6767760350801973E-4 54-55 4.2479271861055307E-4 56-57 0.0016002465427109875 58-59 3.200493085421975E-4 60-61 0.0013616643308886221 62-63 0.0023800030398865232 64-65 9.892433173122467E-4 66-67 5.799805549678286 68-69 5.447937520788657 70-71 0.0032819601821418073 72-73 0.0016526182477451653 74-75 0.0037242101357637526 76-77 0.006529005894260829 78-79 5.415321586709039 80-81 0.006075117783964621 82-83 0.005609591516994153 84-85 5.800271075945257 86-87 0.004416680457882325 88-89 0.0021937925330983354 90-91 0.004207193637745615 92-93 0.004556338337973467 94-95 0.004992769213258281 96-97 0.0035263614723013035 98-99 0.006633749304329185 100 0.00727384792141358 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8592426.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.31791826685196 #Duplication Level Percentage of deduplicated Percentage of total 1 90.09945802747656 76.87098195875905 2 7.035382035346159 12.004882989354845 3 1.529640131884131 3.9151713524936085 4 0.5663058913825952 1.9326415902006806 5 0.2681897021348813 1.144069354337759 6 0.1569217336660111 0.8032941388325671 7 0.10318027740371467 0.616218853199687 8 0.05614344626901402 0.3832033567999306 9 0.041228693305120384 0.3165791657089831 >10 0.14106058119809808 1.8471378201228905 >50 0.0019557042971931763 0.10673803611351455 >100 5.337756366059228E-4 0.059081384076547054 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.1638156674261727E-5 0.0 5 0.0 1.1638156674261727E-5 0.0 1.1638156674261727E-5 0.0 6 0.0 1.1638156674261727E-5 0.0 1.1638156674261727E-5 0.0 7 0.0 1.1638156674261727E-5 0.0 2.3276313348523454E-5 0.0 8 0.0 1.1638156674261727E-5 0.0 2.3276313348523454E-5 0.0 9 0.0 1.1638156674261727E-5 0.0 9.310525339409382E-5 0.0 10-11 0.0 2.3276313348523454E-5 0.0 1.2220064507974812E-4 0.0 12-13 0.0 2.3276313348523454E-5 0.0 1.6293419343966418E-4 0.0 14-15 0.0 2.3276313348523454E-5 0.0 1.9784866346244935E-4 0.0 16-17 0.0 2.3276313348523454E-5 0.0 2.094868201367111E-4 0.0 18-19 0.0 2.3276313348523454E-5 0.0 2.2112497681097283E-4 5.819078337130864E-6 20-21 0.0 2.3276313348523454E-5 0.0 2.4440129015949625E-4 3.491447002278518E-5 22-23 0.0 3.491447002278518E-5 0.0 4.015164052620296E-4 5.819078337130864E-5 24-25 0.0 3.491447002278518E-5 0.0 5.760887553759556E-4 5.819078337130864E-5 26-27 0.0 3.491447002278518E-5 0.0 0.001111443962391995 6.400986170843951E-5 28-29 0.0 3.491447002278518E-5 0.0 0.002752424053462899 8.146709671983209E-5 30-31 0.0 3.491447002278518E-5 0.0 0.007844117598452405 8.146709671983209E-5 32-33 0.0 3.491447002278518E-5 0.0 0.019051662475766448 8.146709671983209E-5 34-35 0.0 3.491447002278518E-5 0.0 0.03777163748631644 8.146709671983209E-5 36-37 0.0 3.491447002278518E-5 0.0 0.0684032658529733 8.146709671983209E-5 38-39 0.0 4.0733548359916046E-5 0.0 0.10909026158619231 9.310525339409382E-5 40-41 0.0 5.819078337130864E-5 0.0 0.1520001452441953 9.310525339409382E-5 42-43 0.0 6.982894004557037E-5 0.0 0.19485183811882698 9.310525339409382E-5 44-45 0.0 6.982894004557037E-5 0.0 0.24896926665414404 1.0474341006835554E-4 46-47 0.0 7.564801838270122E-5 0.0 0.31524856891406455 1.0474341006835554E-4 48-49 0.0 8.146709671983209E-5 0.0 0.3845654300659674 1.0474341006835554E-4 50-51 0.0 8.146709671983209E-5 0.0 0.4546387714016973 1.1056248840548641E-4 52-53 0.0 8.146709671983209E-5 0.0 0.5248692278525297 1.2801972341687902E-4 54-55 0.0 8.146709671983209E-5 0.0 0.6029961736068487 1.3965788009114073E-4 56-57 0.0 8.146709671983209E-5 0.0 0.686831635209893 1.5129603676540245E-4 58-59 0.0 8.146709671983209E-5 0.0 0.781007599018019 1.6293419343966418E-4 60-61 0.0 8.146709671983209E-5 0.0 0.8761437107517713 1.6293419343966418E-4 62-63 0.0 8.146709671983209E-5 0.0 0.9737645689354788 1.6875327177679505E-4 64-65 0.0 8.146709671983209E-5 0.0 1.0783392257320576 1.7457235011392592E-4 66-67 0.0 8.146709671983209E-5 0.0 1.1944822102628525 1.7457235011392592E-4 68-69 0.0 8.146709671983209E-5 0.0 1.3137267635473382 1.7457235011392592E-4 70-71 0.0 8.146709671983209E-5 0.0 1.4545368211492309 1.8039142845105677E-4 72-73 0.0 8.146709671983209E-5 0.0 1.605949239481376 1.8621050678818764E-4 74-75 0.0 8.146709671983209E-5 0.0 1.7455722051024938 1.8621050678818764E-4 76-77 0.0 8.146709671983209E-5 0.0 1.8912295549592164 1.8621050678818764E-4 78-79 0.0 8.146709671983209E-5 0.0 2.0627759843378346 1.8621050678818764E-4 80-81 0.0 8.146709671983209E-5 0.0 2.2702959559965947 1.8621050678818764E-4 82-83 0.0 8.146709671983209E-5 0.0 2.483635005992487 1.8621050678818764E-4 84-85 0.0 8.146709671983209E-5 0.0 2.702601104740384 1.8621050678818764E-4 86-87 0.0 8.146709671983209E-5 0.0 2.9591933640161696 1.8621050678818764E-4 88 0.0 8.146709671983209E-5 0.0 3.173399456684294 1.8621050678818764E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8920 0.0 42.809986 1 GTATCAA 14125 0.0 37.5804 1 TCAACGC 14820 0.0 34.465748 4 ATCAACG 14925 0.0 34.291855 3 CAACGCA 16325 0.0 31.287735 5 TATCAAC 17290 0.0 30.13409 2 AACGCAG 17750 0.0 28.687891 6 ACGCAGA 20355 0.0 24.562607 7 GTACATG 23705 0.0 23.753294 1 CGCAGAG 20575 0.0 23.657463 8 TACATGG 23980 0.0 22.516901 2 ACATGGG 24855 0.0 20.941729 3 GTGGTAT 5365 0.0 20.608913 1 GCAGAGT 23815 0.0 19.26119 9 CATGGGG 17515 0.0 18.438456 4 GTCGTAC 365 8.4946805E-10 18.231466 1 GAGTACA 19005 0.0 17.183987 1 CAGAGTA 22355 0.0 16.927374 10-11 AGAGTAC 20445 0.0 16.78289 10-11 TGGTATC 6200 0.0 16.17967 2 >>END_MODULE