FastQCFastQC Report
Wed 25 May 2016
SRR2049519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049519_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8592426
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292790.34075358926570914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282530.3288128405179166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166150.1933679731428586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174850.057.3607751
GTATCAA308450.039.629721
ATCAACG381450.031.69273
TCAACGC387300.031.2139954
GTGGTAT72800.030.7229771
TATCAAC399450.030.3707662
CAACGCA401500.030.0735935
AACGCAG420050.028.7892516
TGGTATC77900.027.2442192
ACGCAGA471800.025.4708927
CGCAGAG470000.025.378458
GCAGAGT504150.023.1746239
GTACATG235750.022.4899121
GAGTACT360750.021.82771112-13
CAGAGTA491550.021.30192410-11
TACATGG243050.020.9298132
AGAGTAC465750.020.64543710-11
ACATGGG247600.019.7278393
GTACTTT396650.019.65657214-15
AGTACTT376300.019.29586812-13
Produced by FastQC (version 0.11.5)