##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049519_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8592426
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81870940756429	31.0	31.0	34.0	30.0	34.0
2	32.04828263868668	33.0	31.0	34.0	30.0	34.0
3	32.060582191804734	34.0	31.0	34.0	30.0	34.0
4	35.68517063748934	37.0	35.0	37.0	33.0	37.0
5	35.78733992006448	37.0	35.0	37.0	35.0	37.0
6	35.837490250134245	37.0	35.0	37.0	35.0	37.0
7	35.82720118858167	37.0	35.0	37.0	35.0	37.0
8	35.8143823409128	37.0	35.0	37.0	35.0	37.0
9	37.56786535025149	39.0	37.0	39.0	35.0	39.0
10-11	37.52275789165947	39.0	37.0	39.0	35.0	39.0
12-13	37.50090504125377	39.0	37.0	39.0	35.0	39.0
14-15	38.78312807116407	40.0	38.0	41.0	35.0	41.0
16-17	38.794043381927295	40.0	38.0	41.0	35.0	41.0
18-19	38.87177975114362	40.0	38.0	41.0	35.0	41.0
20-21	38.90415989616902	40.0	38.0	41.0	35.0	41.0
22-23	38.881973728956176	40.0	38.0	41.0	35.0	41.0
24-25	38.84434145839603	40.0	38.0	41.0	35.0	41.0
26-27	38.75564520427642	40.0	38.0	41.0	35.0	41.0
28-29	38.674295827511344	40.0	38.0	41.0	34.0	41.0
30-31	38.56133663531114	40.0	38.0	41.0	34.0	41.0
32-33	38.41531349819015	40.0	38.0	41.0	34.0	41.0
34-35	38.31835124329264	40.0	38.0	41.0	34.0	41.0
36-37	38.187615115917204	40.0	38.0	41.0	34.0	41.0
38-39	38.10305209494967	40.0	38.0	41.0	33.5	41.0
40-41	37.9863455908727	40.0	38.0	41.0	33.0	41.0
42-43	37.84853067108172	40.0	37.0	41.0	33.0	41.0
44-45	37.665762789228566	40.0	37.0	41.0	33.0	41.0
46-47	37.419440388546846	40.0	37.0	41.0	32.5	41.0
48-49	37.1902911354721	40.0	37.0	41.0	32.0	41.0
50-51	36.9837532496643	40.0	37.0	41.0	31.0	41.0
52-53	36.89256439333897	40.0	36.5	41.0	31.0	41.0
54-55	36.77287712457459	40.0	36.0	41.0	31.0	41.0
56-57	36.61215162050857	39.5	36.0	41.0	31.0	41.0
58-59	36.450011964025066	39.0	35.0	41.0	31.0	41.0
60-61	36.2722125858285	39.0	35.0	41.0	30.0	41.0
62-63	36.087487806121345	39.0	35.0	41.0	30.0	41.0
64-65	35.82241470569546	38.5	35.0	40.5	30.0	41.0
66-67	35.579187821925956	38.0	35.0	40.0	29.0	41.0
68-69	35.28184531353543	37.0	35.0	40.0	29.0	41.0
70-71	34.99816797956712	37.0	35.0	40.0	29.0	41.0
72-73	34.68110007581095	36.5	34.0	39.0	29.0	41.0
74-75	34.3431063008282	36.0	34.0	39.0	29.0	41.0
76-77	34.04607179625405	35.5	34.0	39.0	28.5	40.0
78-79	33.71429867420447	35.0	34.0	37.5	28.0	39.5
80-81	33.396490700065385	35.0	34.0	37.0	28.0	39.0
82-83	33.09251816658066	35.0	34.0	36.5	28.0	39.0
84-85	32.80292335366054	35.0	34.0	36.0	27.0	37.5
86-87	32.51822162914176	35.0	33.0	36.0	26.5	37.0
88-89	32.3005141388474	35.0	33.0	35.5	26.5	37.0
90-91	32.14268822332598	35.0	33.0	35.0	26.0	36.0
92-93	31.980276233976294	35.0	33.0	35.0	26.0	36.0
94-95	31.7946172594329	35.0	33.0	35.0	26.0	36.0
96-97	31.62793028418284	35.0	33.0	35.0	25.5	36.0
98-99	31.449955926300674	35.0	33.0	35.0	25.0	35.5
100	31.339980466517837	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4130888725717483
1101	2	0.4053369770893056
1101	3	0.47699464322210616
1101	4	0.401694067284609
1101	5	0.3145332799266356
1101	6	0.3135367656477044
1101	7	0.3256414820868585
1101	8	0.31693721846122713
1101	9	0.3538526675784439
1101	10-11	0.34923931233705474
1101	12-13	0.30178713103455124
1101	14-15	0.46759574413569993
1101	16-17	0.47050328927964813
1101	18-19	0.44579436018175755
1101	20-21	0.41774021381861104
1101	22-23	0.4689619951104689
1101	24-25	0.5165955641349456
1101	26-27	0.5087408471232138
1101	28-29	0.5105841742631299
1101	30-31	0.5650285528713113
1101	32-33	0.583836151921858
1101	34-35	0.5998570174793585
1101	36-37	0.6239780918947844
1101	38-39	0.6242680548269348
1101	40-41	0.6167081506026051
1101	42-43	0.5424777207043832
1101	44-45	0.5768682528327886
1101	46-47	0.624879070628154
1101	48-49	0.6356931204121636
1101	50-51	0.66252639059676
1101	52-53	0.6479985101626298
1101	54-55	0.5698315148621731
1101	56-57	0.595968095163478
1101	58-59	0.5660415038875115
1101	60-61	0.6365456672372503
1101	62-63	0.6171693339800299
1101	64-65	0.6200536198538913
1101	66-67	0.6076809410020871
1101	68-69	0.5843155208784196
1101	70-71	0.5565942916238953
1101	72-73	0.559264410649952
1101	74-75	0.581077241914052
1101	76-77	0.5851657761850433
1101	78-79	0.564667985711587
1101	80-81	0.5367256475886677
1101	82-83	0.5753857825016198
1101	84-85	0.5990520233073511
1101	86-87	0.6416072193451328
1101	88-89	0.6227404809277672
1101	90-91	0.6310328239079368
1101	92-93	0.6483357353180956
1101	94-95	0.60846216803834
1101	96-97	0.6231209353457032
1101	98-99	0.6844323919796835
1101	100	0.7398669642353823
1102	1	0.2676059430622857
1102	2	0.327356265041594
1102	3	0.3936660825394114
1102	4	0.33929317919722024
1102	5	0.2791303927713642
1102	6	0.2432739231731489
1102	7	0.2990943860309301
1102	8	0.29256227176641403
1102	9	0.33883452798920644
1102	10-11	0.3364618114040141
1102	12-13	0.33620572451001607
1102	14-15	0.41300522224549496
1102	16-17	0.39494184484571804
1102	18-19	0.36968140062807464
1102	20-21	0.35884821433075587
1102	22-23	0.36577744709046556
1102	24-25	0.38369895499488393
1102	26-27	0.38508985038343724
1102	28-29	0.3570044548541489
1102	30-31	0.426576265276438
1102	32-33	0.4567154256875625
1102	34-35	0.4703566864341511
1102	36-37	0.45709610346990104
1102	38-39	0.4541087699090909
1102	40-41	0.47514219344429875
1102	42-43	0.4890967203100729
1102	44-45	0.5257789075195163
1102	46-47	0.5083427380508851
1102	48-49	0.5029846220014988
1102	50-51	0.5327154719655383
1102	52-53	0.5079906878710432
1102	54-55	0.427677701464404
1102	56-57	0.44060228017047365
1102	58-59	0.4514464841115924
1102	60-61	0.4508116191611933
1102	62-63	0.4715088407658925
1102	64-65	0.4843521011299785
1102	66-67	0.5543885471868748
1102	68-69	0.47987024434932124
1102	70-71	0.4839311927262884
1102	72-73	0.5077600965799007
1102	74-75	0.5212281586407457
1102	76-77	0.4732669829702374
1102	78-79	0.446777107495997
1102	80-81	0.4343212492717612
1102	82-83	0.4454940986417242
1102	84-85	0.4480395770013743
1102	86-87	0.48623400203565836
1102	88-89	0.4774612053106253
1102	90-91	0.47136899934508136
1102	92-93	0.4659757209504569
1102	94-95	0.4765925273580187
1102	96-97	0.49433812189776205
1102	98-99	0.5193572086081346
1102	100	0.5567234444478544
1103	1	0.2887026057642217
1103	2	0.2847037048586287
1103	3	0.36002797622064975
1103	4	0.2856214315893766
1103	5	0.21276969473944263
1103	6	0.2608658428800368
1103	7	0.2296869951985414
1103	8	0.24967096573354297
1103	9	0.3337324834204054
1103	10-11	0.30343562434733684
1103	12-13	0.28454930338210005
1103	14-15	0.3667248133995571
1103	16-17	0.3824620679652071
1103	18-19	0.33879131560244247
1103	20-21	0.34398435046090725
1103	22-23	0.35169673677818025
1103	24-25	0.36222175753414376
1103	26-27	0.3832960314452123
1103	28-29	0.3752409837996922
1103	30-31	0.3978222432296121
1103	32-33	0.42509551944247903
1103	34-35	0.42118272813450375
1103	36-37	0.4539828656578564
1103	38-39	0.4180475894512199
1103	40-41	0.461930187123059
1103	42-43	0.4616553643544421
1103	44-45	0.42556245203552834
1103	46-47	0.41673095480123834
1103	48-49	0.36751177781074773
1103	50-51	0.4565499711372283
1103	52-53	0.4021624161903361
1103	54-55	0.40155151094313624
1103	56-57	0.4595290443847304
1103	58-59	0.3748209576963788
1103	60-61	0.3632363206720939
1103	62-63	0.35934950831649815
1103	64-65	0.38067056670615074
1103	66-67	0.42522876393798015
1103	68-69	0.46688900420427615
1103	70-71	0.39163757043885994
1103	72-73	0.4260360630776958
1103	74-75	0.3942682627430756
1103	76-77	0.3825769691928045
1103	78-79	0.33639016081550466
1103	80-81	0.3534632544454368
1103	82-83	0.35204777473389726
1103	84-85	0.3858502403929833
1103	86-87	0.37738016156025367
1103	88-89	0.3733277063324536
1103	90-91	0.34449333586791653
1103	92-93	0.36041032614766877
1103	94-95	0.36786833705457056
1103	96-97	0.3847703772437754
1103	98-99	0.44207243161980614
1103	100	0.39367056655411403
1104	1	0.29391028240070227
1104	2	0.267013603395462
1104	3	0.35961514328533184
1104	4	0.25479500830183355
1104	5	0.16487131365073537
1104	6	0.22813488256498005
1104	7	0.1541447451490967
1104	8	0.22122994210432267
1104	9	0.22354828326237453
1104	10-11	0.24946410034119282
1104	12-13	0.27820774732450815
1104	14-15	0.34965728795953055
1104	16-17	0.3361930792132597
1104	18-19	0.33005313959140636
1104	20-21	0.32044386120217183
1104	22-23	0.3164177827627839
1104	24-25	0.2979239754124734
1104	26-27	0.32488791128443495
1104	28-29	0.30367372846743734
1104	30-31	0.3689503533767038
1104	32-33	0.3732435004127126
1104	34-35	0.3384043274594646
1104	36-37	0.3892552572728434
1104	38-39	0.38633136105161725
1104	40-41	0.41981891913385994
1104	42-43	0.3865100283291554
1104	44-45	0.3881573147441557
1104	46-47	0.44515778547759055
1104	48-49	0.4511604154282267
1104	50-51	0.4810992783200234
1104	52-53	0.47974226494126526
1104	54-55	0.4115429895330749
1104	56-57	0.44289490049418845
1104	58-59	0.44923537387736445
1104	60-61	0.4257983306573436
1104	62-63	0.45277644831398334
1104	64-65	0.4967475393088918
1104	66-67	0.46358452522748905
1104	68-69	0.41966813535749736
1104	70-71	0.44041643458539426
1104	72-73	0.47452178996643823
1104	74-75	0.508551695291338
1104	76-77	0.4554133050566449
1104	78-79	0.42927599565964414
1104	80-81	0.4506548383846152
1104	82-83	0.4196849071321864
1104	84-85	0.477047007344197
1104	86-87	0.5190019715836769
1104	88-89	0.4333678563198049
1104	90-91	0.4650563653901969
1104	92-93	0.468820303827993
1104	94-95	0.46083626611824613
1104	96-97	0.4318618211454037
1104	98-99	0.4999977000689704
1104	100	0.5264930934986438
1105	1	0.2496645093371157
1105	2	0.2751364558151579
1105	3	0.3335909033742226
1105	4	0.2696385970783268
1105	5	0.1991606243952475
1105	6	0.2272788019868841
1105	7	0.1773776840804615
1105	8	0.22505688393545853
1105	9	0.22926289419938684
1105	10-11	0.22217522700569958
1105	12-13	0.23111501128996537
1105	14-15	0.317499934903438
1105	16-17	0.2950130824986559
1105	18-19	0.28527155257568637
1105	20-21	0.29042805834976804
1105	22-23	0.30808927189615076
1105	24-25	0.28332338089425946
1105	26-27	0.3024209824788002
1105	28-29	0.3358216039248987
1105	30-31	0.3449532308893595
1105	32-33	0.3777686213110627
1105	34-35	0.3134452196383748
1105	36-37	0.3484772510595988
1105	38-39	0.35877472589791637
1105	40-41	0.32948500524972957
1105	42-43	0.3223876805749555
1105	44-45	0.36339868202475145
1105	46-47	0.3852712771507285
1105	48-49	0.39485291325872396
1105	50-51	0.4721691788457676
1105	52-53	0.4213509188658122
1105	54-55	0.42222625121084434
1105	56-57	0.39487857337997667
1105	58-59	0.38210423633085355
1105	60-61	0.433699696375065
1105	62-63	0.4090181516350242
1105	64-65	0.4049522547136064
1105	66-67	0.4164730819736846
1105	68-69	0.42660005220817254
1105	70-71	0.44606750266902395
1105	72-73	0.4534986919638371
1105	74-75	0.4508058783175031
1105	76-77	0.42973940968981594
1105	78-79	0.40192712374186357
1105	80-81	0.3658918593969389
1105	82-83	0.4318065743379549
1105	84-85	0.4773631248746426
1105	86-87	0.49071582745789044
1105	88-89	0.44178747188856704
1105	90-91	0.4194610657468729
1105	92-93	0.4752481055742841
1105	94-95	0.4780668367991119
1105	96-97	0.5043605758119227
1105	98-99	0.5057796521567148
1105	100	0.5321439438454831
1106	1	0.18352206167217133
1106	2	0.2005180638982793
1106	3	0.30342790406690767
1106	4	0.2187227536495584
1106	5	0.18598382715876483
1106	6	0.18109017497791768
1106	7	0.1798113344772574
1106	8	0.17728241702936742
1106	9	0.17375089996369297
1106	10-11	0.20429696320361046
1106	12-13	0.17065654081685722
1106	14-15	0.27670194342847054
1106	16-17	0.28349951564001685
1106	18-19	0.2550248655082967
1106	20-21	0.23357721848587687
1106	22-23	0.2345576670929006
1106	24-25	0.24274151674409694
1106	26-27	0.2676662830291292
1106	28-29	0.24379041256372602
1106	30-31	0.2518534425977208
1106	32-33	0.27954684323933066
1106	34-35	0.2712126815037621
1106	36-37	0.28418177341158213
1106	38-39	0.3059244874256777
1106	40-41	0.34634372924865886
1106	42-43	0.2943311138730351
1106	44-45	0.3015176165116955
1106	46-47	0.24078187277389418
1106	48-49	0.2898011441618351
1106	50-51	0.26907096907216044
1106	52-53	0.22504630800784753
1106	54-55	0.21363856650738455
1106	56-57	0.204489374271553
1106	58-59	0.1894749848628834
1106	60-61	0.24786276675209962
1106	62-63	0.22601055292967942
1106	64-65	0.240334934126885
1106	66-67	0.2541239592090676
1106	68-69	0.2618549394195213
1106	70-71	0.32269660737075867
1106	72-73	0.27226263096967074
1106	74-75	0.2654836815568373
1106	76-77	0.25039431159423486
1106	78-79	0.2141269548937501
1106	80-81	0.18020001967751398
1106	82-83	0.1901946355793811
1106	84-85	0.18907001791596656
1106	86-87	0.26753848263700775
1106	88-89	0.26017756387781077
1106	90-91	0.23580876361772596
1106	92-93	0.21985055037248102
1106	94-95	0.1842555428396686
1106	96-97	0.24661828662352292
1106	98-99	0.2922419389044144
1106	100	0.32349276084122636
1107	1	0.19654490612254705
1107	2	0.18227040858364063
1107	3	0.24982226941735775
1107	4	0.249599099189318
1107	5	0.17185524679486974
1107	6	0.17976785167608256
1107	7	0.16241009170627052
1107	8	0.20825847606622006
1107	9	0.192348505557284
1107	10-11	0.17697093877573167
1107	12-13	0.1458050668445523
1107	14-15	0.19771876005142985
1107	16-17	0.20613260981628656
1107	18-19	0.23808253810515367
1107	20-21	0.242109740838103
1107	22-23	0.23444380594327896
1107	24-25	0.26782103448854144
1107	26-27	0.28396605438109646
1107	28-29	0.29436427042498536
1107	30-31	0.3135651700639883
1107	32-33	0.29404849717188597
1107	34-35	0.22726449107784674
1107	36-37	0.31163669993798493
1107	38-39	0.3307732644394932
1107	40-41	0.30085464239732573
1107	42-43	0.2935846424179118
1107	44-45	0.3043251844435204
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2216	86-87	-1.5021858271336512
2216	88-89	-1.3076648283523156
2216	90-91	-1.1411150672176689
2216	92-93	-1.2544531330481696
2216	94-95	-1.3407474916669777
2216	96-97	-1.4156569751970878
2216	98-99	-1.462909550226719
2216	100	-1.5033187849662752
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	606.0
3	83.0
4	76.0
5	161.0
6	468.0
7	1277.0
8	3051.0
9	5073.0
10	6770.0
11	9104.0
12	12949.0
13	18807.0
14	22961.0
15	30046.0
16	39341.0
17	55974.0
18	57791.0
19	31635.0
20	22814.0
21	21669.0
22	22463.0
23	24763.0
24	28541.0
25	35260.0
26	44655.0
27	58580.0
28	76487.0
29	100151.0
30	130350.0
31	168245.0
32	217701.0
33	282522.0
34	382711.0
35	552576.0
36	897102.0
37	1669102.0
38	2477132.0
39	1078953.0
40	4476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.66015428714831	21.0805573621777	15.737324062984184	22.521964287689812
2	15.091715321080725	20.940102553383493	40.470098993261836	23.49808313227394
3	17.681890465368095	25.81368191327616	31.430059782897118	25.07436783845862
4	12.445369178912465	17.892400106596156	38.6361774627901	31.02605325170128
5	12.419718406900353	37.95308240350381	35.81648405261668	13.81071513697916
6	29.596679612827888	38.86193501563211	17.5296089464798	14.011776425060207
7	25.674006386554854	33.632049900691605	22.698513784116383	17.99542992863715
8	24.407740025925158	36.6963416385547	20.373733797649233	18.522184537870913
9	24.325161328165372	17.1753977690114	22.211427182829897	36.28801371999334
10-11	23.384030307622087	27.625370297049983	28.762220355461892	20.22837903986604
12-13	24.178047576221957	25.166104204023952	29.296301479588553	21.359546740165534
14-15	21.334242894335922	27.135144118536523	27.253527482671213	24.277085504456338
16-17	20.022284742399876	30.468950212664037	28.148895317806637	21.359869727129453
18-19	19.901940383309675	28.615172245882597	31.352542343687333	20.1303450271204
20-21	20.95376788813776	27.69653180603476	31.299187214414182	20.0505130914133
22-23	20.637326408164586	27.529378780800673	31.426025664928623	20.407269146106117
24-25	20.526472965842242	27.660750293339735	31.331936987295556	20.48083975352246
26-27	20.29821379898995	27.74785025788991	31.356615698523328	20.597320244596812
28-29	20.285320399904077	27.732636842711365	31.54403528555811	20.438007471826445
30-31	20.588180183862463	27.94177087405488	31.149296919452436	20.320752022630227
32-33	20.361213510737656	27.7972458299025	31.410527295844886	20.431013363514957
34-35	20.55114003891334	27.653319330303223	31.28062435451874	20.5149162762647
36-37	20.699189029966625	27.825721164197397	30.93352215078722	20.54156765504876
38-39	20.54666516767209	27.958803485767582	30.99946976558192	20.495061580978412
40-41	20.562219564067238	27.784562823118875	30.971066844218388	20.682150768595505
42-43	20.58673534110157	27.81176119526662	30.83820564762501	20.763297816006794
44-45	20.74289030827848	27.94828259213405	30.598279228706772	20.710547870880703
46-47	20.85017083650182	28.066025823207557	30.234819595769576	20.848983744521046
48-49	20.92627274299482	28.1285634580967	29.83776060451379	21.107403194394692
50-51	21.009823069759346	28.22662656623403	29.564799277875654	21.198751086130972
52-53	20.93495480787382	28.374442794153826	29.54000418508114	21.150598212891214
54-55	21.042974359045978	28.53012059690709	29.41687830654579	21.010026737501146
56-57	20.944474820033363	28.3376254855148	29.430791722849868	21.28710797160197
58-59	20.892382336050403	28.07193260047289	29.75561148221083	21.280073581265874
60-61	20.990148765901502	28.269542268970373	29.53213679116934	21.208172173958786
62-63	20.828628608497763	28.270292930075858	29.628169040967013	21.272909420459367
64-65	20.812719248324047	28.184822307460085	29.675338489967796	21.32711995424808
66-67	20.794656829165593	28.11551708446485	29.620121255626756	21.4697048307428
68-69	21.097132521129655	28.300976930147552	29.395429183795123	21.20646136492767
70-71	21.006896073355765	28.196181148374162	29.35515534262384	21.44176743564623
72-73	20.907453843652537	28.261360644828365	29.44937785905866	21.381807652460434
74-75	21.023771400533448	28.32857681870056	29.214129979123477	21.433521801642517
76-77	21.02902020919354	28.270857380674563	29.140117121753505	21.56000528837839
78-79	21.092420656896923	28.32850164857639	29.22674170650656	21.35233598802013
80-81	20.984585863014246	28.29580474243269	29.23100280020569	21.488606594347367
82-83	20.92018216338131	28.295499398799855	29.33638479090205	21.447933646916784
84-85	20.914035221251833	28.23451141738084	29.19182545185725	21.659627909510075
86-87	21.03909425807649	28.270573890922883	29.11534699951719	21.574984851483435
88-89	20.941333065500398	28.18552536008187	29.136497152548742	21.736644421868995
90-91	20.793217558448596	28.1588881458569	29.175435051956246	21.872459243738263
92-93	20.79483043887699	28.302313673864386	29.088621438535	21.814234448723628
94-95	20.722372248729265	28.114709439030822	29.194748992725582	21.96816931951433
96-97	20.751275600162284	28.197816309386898	29.129049234756284	21.921858855694538
98-99	20.70667157098846	28.163038789472793	29.07046634060026	22.05982329893849
100	20.683090775864237	28.089709796925817	29.111272288488056	22.11592713872189
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	957.0
1	791.0
2	1019.0
3	1423.5
4	1002.0
5	742.5
6	1983.5
7	5761.0
8	9935.0
9	11645.5
10	9362.0
11	6166.5
12	5868.0
13	6520.0
14	7289.0
15	8154.0
16	9244.5
17	10652.0
18	12077.0
19	13789.0
20	16327.5
21	20188.5
22	26274.0
23	35690.5
24	46906.5
25	60194.5
26	77719.0
27	99096.0
28	125839.0
29	153314.5
30	175448.0
31	197783.5
32	219464.5
33	237688.5
34	255229.0
35	271819.5
36	285497.5
37	296168.5
38	305035.5
39	309597.0
40	312975.5
41	312732.0
42	306171.5
43	302272.0
44	297712.0
45	290038.5
46	282760.0
47	274413.5
48	264658.5
49	258352.5
50	251815.0
51	238254.0
52	220420.0
53	206204.5
54	196023.5
55	183479.5
56	166185.5
57	147046.0
58	131439.0
59	119124.0
60	103148.0
61	84134.0
62	69418.5
63	56330.5
64	44194.5
65	34569.5
66	26610.5
67	19946.0
68	15191.0
69	11437.0
70	8273.5
71	6000.0
72	4239.0
73	2860.5
74	1874.5
75	1216.5
76	760.0
77	434.0
78	243.5
79	120.5
80	60.5
81	34.0
82	19.0
83	13.0
84	7.0
85	5.0
86	6.0
87	4.5
88	2.5
89	0.5
90	1.0
91	0.5
92	0.5
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	3.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06041367129609263
2	2.6767760350801973E-4
3	2.3276313348523454E-5
4	0.12223555954977093
5	0.0019784866346244934
6	0.0020366774179958022
7	0.0
8	0.0
9	2.3276313348523454E-5
10-11	0.0
12-13	4.0733548359916046E-5
14-15	2.9095391685654317E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.803914284510568E-4
30-31	2.909539168565432E-4
32-33	1.1638156674261727E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.5022036849662715E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.454769584282716E-4
80-81	2.5022036849662715E-4
82-83	3.4332562189072095E-4
84-85	0.0
86-87	5.819078337130864E-6
88-89	5.8190783371308634E-5
90-91	4.655262669704691E-5
92-93	6.110032253987407E-4
94-95	6.459176954215259E-4
96-97	0.0
98-99	5.237170503417778E-5
100	0.0011405393540776492
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8592426.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.60853321414882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.08126276588268	36.86872439908429
2	16.55395652670953	17.417587435219477
3	5.859613485288461	9.24797011988617
4	2.6676921478570534	5.613734838626472
5	1.4059115596999707	3.6981472492315843
6	0.8250835578140553	2.6043861453425285
7	0.5499841784005841	2.0253702641650437
8	0.3830391978808613	1.6120904291228972
9	0.2719243160699793	1.2874985472331986
>10	1.297730692663178	11.86626133826108
>50	0.06917320949650603	2.498124006055167
>100	0.03210325241992619	3.1113675043192677
>500	0.001772268731342699	0.6322995540424065
>1k	6.642715884532863E-4	0.5572544598725324
>5k	2.214237438376946E-5	0.09546173884119918
>10k+	6.64271231513084E-5	0.8637219706965706
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29279	0.34075358926570914	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28253	0.3288128405179166	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16615	0.1933679731428586	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.1638156674261727E-5	0.0
5	0.0	2.3276313348523454E-5	0.0	1.1638156674261727E-5	0.0
6	0.0	2.3276313348523454E-5	0.0	1.1638156674261727E-5	1.1638156674261727E-5
7	0.0	2.3276313348523454E-5	0.0	2.3276313348523454E-5	1.1638156674261727E-5
8	0.0	2.3276313348523454E-5	0.0	2.3276313348523454E-5	4.655262669704691E-5
9	1.1638156674261727E-5	2.3276313348523454E-5	0.0	9.310525339409382E-5	4.655262669704691E-5
10-11	1.1638156674261727E-5	2.3276313348523454E-5	0.0	1.1056248840548641E-4	4.655262669704691E-5
12-13	1.1638156674261727E-5	2.3276313348523454E-5	0.0	1.6293419343966418E-4	4.655262669704691E-5
14-15	1.1638156674261727E-5	2.3276313348523454E-5	0.0	2.094868201367111E-4	4.655262669704691E-5
16-17	1.1638156674261727E-5	2.909539168565432E-5	0.0	2.269440551481037E-4	5.819078337130864E-5
18-19	1.1638156674261727E-5	3.491447002278518E-5	0.0	2.444012901594963E-4	5.819078337130864E-5
20-21	1.1638156674261727E-5	3.491447002278518E-5	0.0	2.851348385194123E-4	6.982894004557037E-5
22-23	1.1638156674261727E-5	4.655262669704691E-5	0.0	4.6552626697046913E-4	8.146709671983209E-5
24-25	1.1638156674261727E-5	4.655262669704691E-5	0.0	6.575558520957876E-4	8.146709671983209E-5
26-27	1.1638156674261727E-5	4.655262669704691E-5	0.0	0.0012103682941232196	8.728617505696296E-5
28-29	1.1638156674261727E-5	4.655262669704691E-5	0.0	0.002892081933554039	9.310525339409382E-5
30-31	1.1638156674261727E-5	4.655262669704691E-5	0.0	0.008053604418589115	9.310525339409382E-5
32-33	1.1638156674261727E-5	4.655262669704691E-5	0.0	0.019296063765925943	9.310525339409382E-5
34-35	1.1638156674261727E-5	4.655262669704691E-5	0.0	0.03821388743993838	9.310525339409382E-5
36-37	1.1638156674261727E-5	4.655262669704691E-5	0.0	0.0690433644700577	9.310525339409382E-5
38-39	1.1638156674261727E-5	5.237170503417777E-5	0.0	0.11000385688512185	9.310525339409382E-5
40-41	1.1638156674261727E-5	5.819078337130864E-5	0.0	0.15317559906829573	9.310525339409382E-5
42-43	1.1638156674261727E-5	5.819078337130864E-5	0.0	0.19635316032980674	9.892433173122467E-5
44-45	1.745723501139259E-5	5.819078337130864E-5	0.0	0.2508488289570373	1.2220064507974812E-4
46-47	2.3276313348523454E-5	6.400986170843951E-5	0.0	0.3176227528756139	1.28019723416879E-4
48-49	2.3276313348523454E-5	6.982894004557037E-5	0.0	0.38723638702271046	1.28019723416879E-4
50-51	2.3276313348523454E-5	6.982894004557037E-5	0.0	0.45774615923372514	1.3383880175400986E-4
52-53	3.491447002278518E-5	6.982894004557037E-5	0.0	0.5280639018596145	1.5129603676540247E-4
54-55	3.491447002278518E-5	6.982894004557037E-5	0.0	0.6088734427273508	1.5129603676540247E-4
56-57	3.491447002278518E-5	6.982894004557037E-5	0.0	0.7046962057048847	1.7457235011392592E-4
58-59	3.491447002278518E-5	6.982894004557037E-5	0.0	0.8125819180752909	2.3276313348523456E-4
60-61	3.491447002278518E-5	6.982894004557037E-5	0.0	0.9223588309052646	2.3276313348523456E-4
62-63	3.491447002278518E-5	6.982894004557037E-5	0.0	1.0339862106464461	2.3276313348523456E-4
64-65	3.491447002278518E-5	6.982894004557037E-5	0.0	1.154220007248244	2.3276313348523456E-4
66-67	3.491447002278518E-5	6.982894004557037E-5	0.0	1.2924522131467877	2.3276313348523456E-4
68-69	3.491447002278518E-5	6.982894004557037E-5	0.0	1.4501666933180455	2.444012901594963E-4
70-71	3.491447002278518E-5	8.146709671983209E-5	0.0	1.615463432562585	2.5022036849662715E-4
72-73	3.491447002278518E-5	8.146709671983209E-5	0.0	1.7875277599132073	2.56039446833758E-4
74-75	3.491447002278518E-5	8.728617505696296E-5	0.0	1.9661618267064505	2.56039446833758E-4
76-77	3.491447002278518E-5	9.310525339409382E-5	0.0	2.159436694595915	2.56039446833758E-4
78-79	3.491447002278518E-5	9.310525339409382E-5	0.0	2.3774659217315346	2.56039446833758E-4
80-81	3.491447002278518E-5	9.310525339409382E-5	0.0	2.615262557978387	2.618585251708889E-4
82-83	3.491447002278518E-5	9.310525339409382E-5	0.0	2.859611476432849	2.6767760350801973E-4
84-85	3.491447002278518E-5	9.310525339409382E-5	0.0	3.1104137527631894	2.6767760350801973E-4
86-87	3.491447002278518E-5	9.310525339409382E-5	0.0	3.3765900340602295	2.6767760350801973E-4
88	3.491447002278518E-5	9.310525339409382E-5	0.0	3.5920355904141625	2.6767760350801973E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17485	0.0	57.360775	1
GTATCAA	30845	0.0	39.62972	1
ATCAACG	38145	0.0	31.6927	3
TCAACGC	38730	0.0	31.213995	4
GTGGTAT	7280	0.0	30.722977	1
TATCAAC	39945	0.0	30.370766	2
CAACGCA	40150	0.0	30.073593	5
AACGCAG	42005	0.0	28.789251	6
TGGTATC	7790	0.0	27.244219	2
ACGCAGA	47180	0.0	25.470892	7
CGCAGAG	47000	0.0	25.37845	8
GCAGAGT	50415	0.0	23.174623	9
GTACATG	23575	0.0	22.489912	1
GAGTACT	36075	0.0	21.827711	12-13
CAGAGTA	49155	0.0	21.301924	10-11
TACATGG	24305	0.0	20.929813	2
AGAGTAC	46575	0.0	20.645437	10-11
ACATGGG	24760	0.0	19.727839	3
GTACTTT	39665	0.0	19.656572	14-15
AGTACTT	37630	0.0	19.295868	12-13
>>END_MODULE