FastQCFastQC Report
Wed 25 May 2016
SRR2049518_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049518_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7253973
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72350.044.435031
GTATCAA117500.034.4186361
TCAACGC122450.032.1443144
ATCAACG123550.032.0238383
CAACGCA133600.029.5763155
TATCAAC141500.028.1423132
AACGCAG146800.026.8469246
GTACATG217000.024.2514531
ACGCAGA169400.023.1434617
TACATGG217150.022.934522
CGCAGAG171050.022.3735628
GTGGTAT42650.022.0852931
ACATGGG225400.021.0959193
CATGGGG159750.018.3270194
TGGTATC47300.018.1796192
GCAGAGT201200.018.0540839
AGAGTAC172200.016.6418410-11
CAGAGTA188800.016.44850510-11
GAGTACA163900.016.2103651
TATACCG4851.364242E-1015.8300685
Produced by FastQC (version 0.11.5)