##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049518_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7253973 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.747081909458444 31.0 31.0 34.0 27.0 34.0 2 30.86409737119231 31.0 31.0 34.0 27.0 34.0 3 31.05234317800742 31.0 31.0 34.0 27.0 34.0 4 34.62080641877217 37.0 35.0 37.0 32.0 37.0 5 34.628733109428445 37.0 35.0 37.0 32.0 37.0 6 34.58307082201712 37.0 35.0 37.0 31.0 37.0 7 30.693879202472907 37.0 33.0 37.0 2.0 37.0 8 32.45827355574662 37.0 33.0 37.0 17.0 37.0 9 35.22603778646543 39.0 34.0 39.0 28.0 39.0 10-11 35.766703639509 39.0 36.0 39.0 30.0 39.0 12-13 35.918297600501134 39.0 37.0 39.0 30.0 39.0 14-15 37.11207320457355 40.0 37.0 41.0 31.0 41.0 16-17 37.12964667775852 40.0 37.0 41.0 31.0 41.0 18-19 37.12649157916634 40.0 37.0 41.0 31.0 41.0 20-21 37.20047207233884 40.0 37.0 41.0 31.0 41.0 22-23 37.16598441984827 40.0 37.0 41.0 31.0 41.0 24-25 37.142568631010896 40.0 37.0 41.0 31.0 41.0 26-27 37.17325912296613 40.0 37.0 41.0 31.0 41.0 28-29 37.16481699063396 40.0 37.0 41.0 31.0 41.0 30-31 37.090584359770844 40.0 37.0 41.0 30.0 41.0 32-33 37.04952231004995 40.0 37.0 41.0 30.0 41.0 34-35 36.996485512146236 40.0 37.0 41.0 30.0 41.0 36-37 36.82836281579763 40.0 37.0 41.0 30.0 41.0 38-39 36.7209968247745 40.0 37.0 41.0 30.0 41.0 40-41 36.649262755734 40.0 36.5 41.0 30.0 41.0 42-43 36.532257564234115 40.0 36.0 41.0 30.0 41.0 44-45 36.41285796073407 40.0 36.0 41.0 30.0 41.0 46-47 36.2613686320586 40.0 36.0 41.0 29.0 41.0 48-49 36.18362592471739 40.0 35.5 41.0 29.0 41.0 50-51 36.049060356304054 39.5 35.0 41.0 29.0 41.0 52-53 35.93394943708779 39.0 35.0 41.0 28.5 41.0 54-55 35.818088032585734 39.0 35.0 41.0 28.0 41.0 56-57 35.62102815932731 39.0 35.0 41.0 28.0 41.0 58-59 35.44224530474541 39.0 35.0 41.0 28.0 41.0 60-61 35.22503702453814 38.5 34.5 40.0 28.0 41.0 62-63 34.99098817985675 38.0 34.0 40.0 27.5 41.0 64-65 34.63594253797195 37.5 34.0 40.0 26.5 41.0 66-67 31.146612897511474 36.0 30.0 40.0 6.5 41.0 68-69 31.50252227296683 36.0 30.0 39.5 11.0 41.0 70-71 31.661751360254577 35.5 30.0 39.0 15.5 41.0 72-73 32.89954766856728 35.0 32.0 39.0 24.0 41.0 74-75 32.98250331232278 35.0 33.0 38.5 25.0 40.0 76-77 32.68383594755591 35.0 33.0 37.5 24.5 39.5 78-79 29.320295650397377 35.0 29.0 37.0 6.5 39.0 80-81 31.099989619481626 35.0 30.0 36.0 19.5 39.0 82-83 31.510033329321736 35.0 32.0 36.0 21.5 37.5 84-85 29.681810161135147 35.0 30.5 35.5 11.0 37.0 86-87 29.31733086130869 35.0 28.5 35.0 12.5 37.0 88-89 30.56256764396559 34.5 30.5 35.0 19.5 36.0 90-91 30.71770807528509 35.0 32.0 35.0 18.5 36.0 92-93 30.575558111396333 35.0 32.0 35.0 16.5 36.0 94-95 30.377654976107575 35.0 32.0 35.0 8.5 36.0 96-97 30.19521757249441 35.0 32.0 35.0 2.0 35.0 98-99 30.015444433002436 35.0 32.0 35.0 2.0 35.0 100 29.816291430916547 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3237127268895925 1101 2 0.34882549772244076 1101 3 0.3736926104538192 1101 4 0.37408633555109105 1101 5 0.35383334680822287 1101 6 0.37437418814314327 1101 7 -12.020489145453183 1101 8 -6.471850895376335 1101 9 -2.106880985208768 1101 10-11 -0.06430601116643686 1101 12-13 0.42201401337025857 1101 14-15 0.5963236275722323 1101 16-17 0.6722309513222484 1101 18-19 0.6175970789090925 1101 20-21 0.5623951753088221 1101 22-23 0.5203608190524989 1101 24-25 0.5991751244874663 1101 26-27 0.5381520676624945 1101 28-29 0.5114632974247542 1101 30-31 0.5816529170446287 1101 32-33 0.6107395609695203 1101 34-35 0.6029063147606379 1101 36-37 0.6320891149194523 1101 38-39 0.6547544444041549 1101 40-41 0.6062073659704623 1101 42-43 0.5786616164437461 1101 44-45 0.5266586121121293 1101 46-47 0.5246223859628785 1101 48-49 0.4399979166742014 1101 50-51 0.5485283781152503 1101 52-53 0.5317401274835873 1101 54-55 0.47393608326027703 1101 56-57 0.4057248641790352 1101 58-59 0.39697429703546305 1101 60-61 0.4016149845641266 1101 62-63 0.445656787134574 1101 64-65 0.3251354235149009 1101 66-67 -10.131588131475368 1101 68-69 -8.070960085466258 1101 70-71 -6.563834061123924 1101 72-73 -1.4527476425306318 1101 74-75 -0.17775351411759743 1101 76-77 0.10167345756804735 1101 78-79 3.211436723820544 1101 80-81 1.0786215154562342 1101 82-83 0.39563630332197874 1101 84-85 -5.296813107878702 1101 86-87 -5.806675066459302 1101 88-89 -1.2709315731838693 1101 90-91 -0.04952147081742808 1101 92-93 0.2560911526772074 1101 94-95 0.36175128437302106 1101 96-97 0.4446702200643422 1101 98-99 0.4532770662395258 1101 100 0.511204822307544 1102 1 0.24314395366120678 1102 2 0.2623616650464342 1102 3 0.294623165344543 1102 4 0.3015808667856845 1102 5 0.3374497681329487 1102 6 0.3104286524451112 1102 7 -12.073531472839093 1102 8 -6.5822408940858885 1102 9 -2.2472424849535813 1102 10-11 -0.21229403631433286 1102 12-13 0.3353590705461258 1102 14-15 0.47309642775294236 1102 16-17 0.5473018772322575 1102 18-19 0.5075322604717911 1102 20-21 0.486933386206573 1102 22-23 0.49408645387234174 1102 24-25 0.5447484951919961 1102 26-27 0.4700266461499396 1102 28-29 0.5100567365823281 1102 30-31 0.5059135164240658 1102 32-33 0.5603804414967897 1102 34-35 0.5578440199678667 1102 36-37 0.6905687719733606 1102 38-39 0.6668219962055772 1102 40-41 0.5822620884393714 1102 42-43 0.5219322906477544 1102 44-45 0.515118767425129 1102 46-47 0.5157777837845146 1102 48-49 0.4565431151872872 1102 50-51 0.5628496818671351 1102 52-53 0.5295822467989169 1102 54-55 0.49557419416033355 1102 56-57 0.49946088959038093 1102 58-59 0.48059710591602567 1102 60-61 0.43317959956704044 1102 62-63 0.3784465258852734 1102 64-65 0.2595468203820275 1102 66-67 -10.282671702699133 1102 68-69 -6.6633651995118335 1102 70-71 -6.255580971138961 1102 72-73 -1.0488187511656761 1102 74-75 -0.11664582098821796 1102 76-77 0.05751177821860409 1102 78-79 3.218370866555805 1102 80-81 1.0662948602146898 1102 82-83 0.3983651828386776 1102 84-85 -5.451993828883527 1102 86-87 -5.925613065015227 1102 88-89 -1.3899569806526806 1102 90-91 -0.10688197807741062 1102 92-93 0.23644892609032553 1102 94-95 0.2863842562449683 1102 96-97 0.3651195359576924 1102 98-99 0.3824861725152253 1102 100 0.4826491651179339 1103 1 0.34525449687379606 1103 2 0.354467867089852 1103 3 0.3602653237933211 1103 4 0.35652893170622235 1103 5 0.3918388583457997 1103 6 0.3806489143408811 1103 7 -12.144716308478678 1103 8 -6.54354849682619 1103 9 -2.1902160361151743 1103 10-11 -0.14858526714328235 1103 12-13 0.4271815667521466 1103 14-15 0.62313304605469 1103 16-17 0.5854973464522715 1103 18-19 0.6295414791588598 1103 20-21 0.6234564770108761 1103 22-23 0.6413780932742412 1103 24-25 0.6467446312661593 1103 26-27 0.6048637372649139 1103 28-29 0.6312830513354228 1103 30-31 0.6505850020874604 1103 32-33 0.6873960259454535 1103 34-35 0.690657898403984 1103 36-37 0.7234825459644796 1103 38-39 0.7394368604784631 1103 40-41 0.669708390406683 1103 42-43 0.6579852139804174 1103 44-45 0.6433211767730782 1103 46-47 0.658119999960789 1103 48-49 0.5703646628358285 1103 50-51 0.5844304834841694 1103 52-53 0.5766098268435087 1103 54-55 0.5758864810861297 1103 56-57 0.5909902314881208 1103 58-59 0.5847806072842232 1103 60-61 0.5534393175687384 1103 62-63 0.5018235633918522 1103 64-65 0.386512099997006 1103 66-67 -10.279667685602725 1103 68-69 -8.192961935604455 1103 70-71 -6.676167216548201 1103 72-73 -1.4503247488610853 1103 74-75 -0.25096401065858487 1103 76-77 0.0375659722610564 1103 78-79 3.19342768649938 1103 80-81 1.1299826861298357 1103 82-83 0.42173986545255815 1103 84-85 -5.469656838592641 1103 86-87 -5.974209832375902 1103 88-89 -1.4045195914041884 1103 90-91 -0.08621813494511343 1103 92-93 0.3362516558155626 1103 94-95 0.40478359969417355 1103 96-97 0.5128841862289981 1103 98-99 0.5306027701699882 1103 100 0.6315057267392952 1104 1 0.1407275339460412 1104 2 0.15426283848022848 1104 3 0.18418024193500315 1104 4 0.208342748965336 1104 5 0.19892858934603908 1104 6 0.16313879409790388 1104 7 -12.218094862249703 1104 8 -6.705291050166672 1104 9 -2.445624122387656 1104 10-11 -0.43125426038081116 1104 12-13 0.14640148554662602 1104 14-15 0.34935582554273026 1104 16-17 0.36326521029522496 1104 18-19 0.3669143996578015 1104 20-21 0.39730512979130594 1104 22-23 0.3688008041328601 1104 24-25 0.49220397648200986 1104 26-27 0.4455313357061357 1104 28-29 0.39264441766270153 1104 30-31 0.37231311853396676 1104 32-33 0.4194289343984252 1104 34-35 0.37435642253110757 1104 36-37 0.4294199850160183 1104 38-39 0.4469895266652699 1104 40-41 0.47619730949506334 1104 42-43 0.3778772874074505 1104 44-45 0.3721476745359098 1104 46-47 0.39581496116063164 1104 48-49 0.3485379453227324 1104 50-51 0.3349148743201411 1104 52-53 0.3768260220619766 1104 54-55 0.3560060757355359 1104 56-57 0.381624285328904 1104 58-59 0.3067909156670936 1104 60-61 0.2882025917294868 1104 62-63 0.2599071763082961 1104 64-65 0.11699206179950039 1104 66-67 -10.448186472101284 1104 68-69 -8.396605052934799 1104 70-71 -6.8783510989855685 1104 72-73 -1.7857972325280187 1104 74-75 -0.5568144003078643 1104 76-77 -0.25276140727881824 1104 78-79 2.872960680470488 1104 80-81 0.7540031542462557 1104 82-83 0.07899898504381042 1104 84-85 -5.631445025484599 1104 86-87 -6.11461379318191 1104 88-89 -1.5930472037324854 1104 90-91 -0.25537327487158734 1104 92-93 0.08825332641904993 1104 94-95 0.16516797281758855 1104 96-97 0.2603827673443355 1104 98-99 0.3050520378571555 1104 100 0.3917835648623331 1105 1 0.19087512376813365 1105 2 0.20990913745229278 1105 3 0.20344858788195452 1105 4 0.2374109992684481 1105 5 0.2538360631134253 1105 6 0.31999931690819494 1105 7 -12.084093218611496 1105 8 -6.552406328352227 1105 9 -2.274550744582932 1105 10-11 -0.24534322283312093 1105 12-13 0.3080058875567815 1105 14-15 0.4753986020177621 1105 16-17 0.4913196913888953 1105 18-19 0.47386086517323633 1105 20-21 0.46620617858292235 1105 22-23 0.4961106478846631 1105 24-25 0.5192710994014433 1105 26-27 0.4745856753426523 1105 28-29 0.46598534096342803 1105 30-31 0.4794925610047045 1105 32-33 0.4530616355336292 1105 34-35 0.5072242293168614 1105 36-37 0.5779349412262675 1105 38-39 0.5317919417792183 1105 40-41 0.5355064942530774 1105 42-43 0.49652627748450584 1105 44-45 0.4315776107177882 1105 46-47 0.4603116493108317 1105 48-49 0.42835414503118585 1105 50-51 0.4966903283826767 1105 52-53 0.4595889112240883 1105 54-55 0.4759743995878267 1105 56-57 0.44185779221436405 1105 58-59 0.46309223395383015 1105 60-61 0.3978690733479624 1105 62-63 0.3537321865329588 1105 64-65 0.20144726582047667 1105 66-67 -10.22307904371985 1105 68-69 -8.232131869751196 1105 70-71 -6.659442079873468 1105 72-73 -1.5588325271585006 1105 74-75 -0.33598649982703677 1105 76-77 -0.033114033061373505 1105 78-79 3.1242909974755655 1105 80-81 1.0211968877366928 1105 82-83 0.3129113952815459 1105 84-85 -5.414098018243788 1105 86-87 -5.912324430803277 1105 88-89 -1.4199156028433997 1105 90-91 -0.1082507101463861 1105 92-93 0.2568041512083248 1105 94-95 0.3095093444221355 1105 96-97 0.3807847791985317 1105 98-99 0.42439762967161343 1105 100 0.4961289494057226 1106 1 0.09304659717522057 1106 2 0.10097720174573865 1106 3 0.11722690343878028 1106 4 0.0773659140069256 1106 5 0.08775921160290778 1106 6 0.11074597929657415 1106 7 -12.221415193267568 1106 8 -6.69939587472771 1106 9 -2.602119336925867 1106 10-11 -0.47782808452988235 1106 12-13 0.08711621295528715 1106 14-15 0.22238573981806553 1106 16-17 0.2262680152042691 1106 18-19 0.2573371413200505 1106 20-21 0.22859429720402602 1106 22-23 0.25784491999078796 1106 24-25 0.33985294584783077 1106 26-27 0.25962262656001656 1106 28-29 0.24985474556773113 1106 30-31 0.23922169427280693 1106 32-33 0.2752279978625296 1106 34-35 0.3143850253877005 1106 36-37 0.35158308758272483 1106 38-39 0.35522109149911074 1106 40-41 0.31992372724108264 1106 42-43 0.29954036438345355 1106 44-45 0.22884148972404006 1106 46-47 0.29543655434562055 1106 48-49 0.2603135291054457 1106 50-51 0.2948587090159336 1106 52-53 0.24958979300754436 1106 54-55 0.23967110935166147 1106 56-57 0.25572947067692553 1106 58-59 0.21441635836531248 1106 60-61 0.18713101243060493 1106 62-63 0.11730687542873142 1106 64-65 -0.03461692229986113 1106 66-67 -10.460188512645349 1106 68-69 -8.445570433325944 1106 70-71 -6.921454236238169 1106 72-73 -1.8107281166491127 1106 74-75 -0.5791558826725094 1106 76-77 -0.2954691052145293 1106 78-79 2.8164438187131573 1106 80-81 0.7119222607698745 1106 82-83 0.02229816387584549 1106 84-85 -5.700476035545524 1106 86-87 -6.172315588474731 1106 88-89 -1.673949741743069 1106 90-91 -0.36608160040884385 1106 92-93 -0.022312991682643002 1106 94-95 0.04147681151140503 1106 96-97 0.13304153249634254 1106 98-99 0.1689026082781595 1106 100 0.27719350697699596 1107 1 0.16864462028879146 1107 2 0.1623192022699591 1107 3 0.13805703203894737 1107 4 0.13076493760235763 1107 5 0.1750311580137165 1107 6 0.15965520755861462 1107 7 -12.122876535666197 1107 8 -6.633118266648182 1107 9 -2.448562505103574 1107 10-11 -0.41077076686207903 1107 12-13 0.24561132758839221 1107 14-15 0.3763496245056359 1107 16-17 0.38697981789016467 1107 18-19 0.40277274935307616 1107 20-21 0.39277827102218765 1107 22-23 0.4392807885427459 1107 24-25 0.4854585440747812 1107 26-27 0.42471070934771404 1107 28-29 0.36767084850391996 1107 30-31 0.4311720096822427 1107 32-33 0.38340797376612556 1107 34-35 0.38090455300127246 1107 36-37 0.4470175490776782 1107 38-39 0.47053512190849034 1107 40-41 0.416741319721325 1107 42-43 0.43563014245507503 1107 44-45 0.3447764421602173 1107 46-47 0.42674591356777825 1107 48-49 0.3518798338801048 1107 50-51 0.36204697884856785 1107 52-53 0.3322309915602304 1107 54-55 0.28670156777261013 1107 56-57 0.30937460981728293 1107 58-59 0.30099495976084256 1107 60-61 0.2913513792782041 1107 62-63 0.24482513484380064 1107 64-65 0.11532305961669209 1107 66-67 -10.288325576176618 1107 68-69 -8.292736047884684 1107 70-71 -6.805417839068461 1107 72-73 -1.7462836000152322 1107 74-75 -0.4802233653558048 1107 76-77 -0.1878414182945889 1107 78-79 2.94803496355372 1107 80-81 0.8623894909600054 1107 82-83 0.19402444733763957 1107 84-85 -5.517464385763223 1107 86-87 -6.034764038052693 1107 88-89 -1.540680980797216 1107 90-91 -0.2711746419737153 1107 92-93 0.10337075903832371 1107 94-95 0.13232080526022472 1107 96-97 0.18650273985825905 1107 98-99 0.2838889358115999 1107 100 0.3895841121166086 1108 1 0.02682794748393391 1108 2 0.028004545783719692 1108 3 0.02335290503606302 1108 4 -0.06598551018441157 1108 5 -0.1089895961575067 1108 6 0.003032103536391162 1108 7 -12.33951175547833 1108 8 -6.808310870006743 1108 9 -2.6920100577033423 1108 10-11 -0.6372645410686388 1108 12-13 -0.0740445529890863 1108 14-15 0.06472279146233717 1108 16-17 0.03190251841028413 1108 18-19 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10-11 -0.724719521156068 2216 12-13 -1.0609255609916346 2216 14-15 -1.2815021427762758 2216 16-17 -1.4207439454353477 2216 18-19 -1.883300810866693 2216 20-21 -1.4726776436798303 2216 22-23 -1.2845677733546381 2216 24-25 -1.2942947920714332 2216 26-27 -1.696734687427778 2216 28-29 -1.908846193717622 2216 30-31 -1.7956581711227741 2216 32-33 -2.732564346125187 2216 34-35 -1.784375304553535 2216 36-37 -1.4541393355128491 2216 38-39 -1.6857943175307 2216 40-41 -1.716507489635056 2216 42-43 -1.4025313300546358 2216 44-45 -1.532013613885745 2216 46-47 -1.7364026436414903 2216 48-49 -1.7842921537984253 2216 50-51 -2.343764147981517 2216 52-53 -2.0706993798029174 2216 54-55 -1.703138434176168 2216 56-57 -1.7310889575449693 2216 58-59 -2.0302020863582726 2216 60-61 -2.2159096164225147 2216 62-63 -2.0632891439630683 2216 64-65 -1.7554737454781062 2216 66-67 1.625393368500859 2216 68-69 0.8436952569651233 2216 70-71 0.5115413522590799 2216 72-73 -1.2342036575227908 2216 74-75 -1.996390816592502 2216 76-77 -1.6905619734778483 2216 78-79 0.8019243764085324 2216 80-81 -0.5788841782814487 2216 82-83 -1.4519566191076763 2216 84-85 0.3637329591083933 2216 86-87 0.32813257887397995 2216 88-89 -0.9225907814419685 2216 90-91 -1.2081924439458476 2216 92-93 -1.2488223371131006 2216 94-95 -1.2641847326919127 2216 96-97 -1.2658889807447231 2216 98-99 -1.3302712663105503 2216 100 -2.582961084540674 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 116352.0 3 22407.0 4 13093.0 5 11533.0 6 10753.0 7 11814.0 8 12820.0 9 13821.0 10 15307.0 11 17164.0 12 19412.0 13 21648.0 14 24743.0 15 27059.0 16 29859.0 17 30458.0 18 28421.0 19 27079.0 20 26224.0 21 26648.0 22 28387.0 23 33107.0 24 39861.0 25 50127.0 26 61890.0 27 74747.0 28 91898.0 29 117793.0 30 144255.0 31 176917.0 32 235639.0 33 332557.0 34 505928.0 35 706795.0 36 769884.0 37 1312574.0 38 1534266.0 39 529505.0 40 1228.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99713864421484 20.55282684357655 14.516320289841882 22.933714222366724 2 15.222513978595128 20.657440290695774 40.60941284455019 23.510632886158906 3 18.126643696785997 25.72115182468006 30.66825092947232 25.48395354906162 4 12.763363648933849 17.493283865934565 38.65715367708599 31.0861988080456 5 13.036416897453654 37.989768952920286 35.269526895064566 13.704287254561503 6 30.093431763049594 38.09102083424092 18.087976843613326 13.727570559096156 7 26.643995191605057 33.06017964879751 22.629933740292707 17.665891419304735 8 24.323360453643815 37.01628059547506 20.700173546275952 17.960185404605173 9 24.549250459024314 17.959826980332018 21.890114562047582 35.60080799859608 10-11 23.460378195507484 27.737303406009367 28.918132173913524 19.884186224569625 12-13 23.820132774136322 25.29264997264258 28.955642652653935 21.931574600567167 14-15 21.379297937833517 27.621022300469 26.129715398720123 24.869964362977363 16-17 20.99298568511245 30.799626934368117 26.867259865192096 21.340127515327335 18-19 20.982715265138154 29.828426436106113 28.610617933096798 20.578240365658928 20-21 21.99632146360642 29.016992345710733 28.216203481347872 20.77048270933497 22-23 21.786061238441334 28.815057624283963 28.264345621358117 21.13453551591659 24-25 21.596909721059067 28.892442803412692 28.197182426788743 21.313465048739495 26-27 21.300791926283555 28.97772498820644 28.20171415384539 21.51976893166462 28-29 21.339981552178372 28.973639686830925 28.407205265307717 21.279173495682986 30-31 21.61347995091793 29.215231432485346 28.005956184286873 21.165332432309853 32-33 21.31528960214746 29.069809920610357 28.3255415070012 21.289358970240983 34-35 21.511165168899687 28.79985436931014 28.30351205833554 21.385468403454635 36-37 21.751186505867306 28.93552390467699 28.002861882135534 21.310427707320166 38-39 21.53489002060595 29.071174449586074 28.136231561396187 21.25770396841179 40-41 21.472047111286464 28.873494566356943 28.244335897031874 21.410122425324715 42-43 21.376335518228114 28.935124857448795 28.210818279225624 21.47772134509746 44-45 21.499658221141157 29.012872211376422 28.13389018783466 21.353579379647762 46-47 21.499190261215286 29.058500740810178 28.018588666138445 21.423720331836087 48-49 21.46276035335675 28.909366258815673 28.092517758167983 21.535355629659595 50-51 21.39358700458406 28.866137276384528 28.103469251376062 21.636806467655347 52-53 21.418030871027778 28.94997190152928 28.076682597979158 21.55531462946378 54-55 21.447757066575424 29.122326383269336 28.0032790464986 21.426637503656636 56-57 21.375044640121523 28.946353895510168 27.98066597650391 21.6979354878644 58-59 21.306522085119056 28.721066349340507 28.21662362916843 21.755787936372005 60-61 21.488153880819674 28.973209968439622 27.948647022863028 21.58998912787768 62-63 21.357331423345794 29.020901391057198 27.980908488027552 21.64085869756946 64-65 21.380016695868235 28.852324142472234 28.032908685346158 21.734750476313366 66-67 21.290255397914088 28.843054166990484 28.00327781853829 21.86341261655714 68-69 21.70843019245839 28.95323756637967 27.80180603444379 21.53652620671815 70-71 21.47091317958811 28.764193383488667 27.95716742774616 21.80772600917707 72-73 21.315391246339708 28.87921556766283 28.126850308739197 21.678542877258263 74-75 21.359103509542983 28.928032393914947 27.999073025823485 21.713791070718592 76-77 21.41811485334428 28.898240737964294 27.848294625984053 21.835349782707375 78-79 21.51863874878174 28.91953424589253 27.964448581060765 21.597378424264964 80-81 21.35454590009651 28.879157535768275 28.024125630959983 21.74217093317523 82-83 21.32403610997546 28.85499658202482 28.10241476482559 21.718552543174123 84-85 21.405843663312858 28.76300542671982 27.94532753767043 21.885823372296894 86-87 21.46275330055161 28.798239944767012 27.96050146436904 21.77850529031233 88-89 21.358105508821883 28.716245068712503 28.02916480930954 21.89648461315608 90-91 21.328363069077287 28.6540320543857 27.96147937210285 22.056125504434167 92-93 21.41805845410748 28.764326291058453 27.90568629024231 21.91192896459176 94-95 21.41458229649043 28.564028294954635 28.014955353142373 22.006434055412562 96-97 21.378522968466783 28.601466509983066 28.000137585260127 22.019872936290028 98-99 21.37943237918298 28.61534942797715 27.94174070365504 22.06347748918483 100 21.454802335829942 28.614027084256172 27.85820629359398 22.07296428631991 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5046.0 1 3963.0 2 3450.5 3 3977.5 4 3672.0 5 3193.5 6 2868.5 7 2692.5 8 2674.0 9 2711.0 10 2470.5 11 2182.5 12 2127.5 13 2199.5 14 2371.5 15 2582.0 16 2935.5 17 3576.5 18 4563.5 19 5991.5 20 8036.5 21 11150.5 22 16097.5 23 23545.0 24 32783.0 25 44148.5 26 59214.0 27 77849.0 28 99793.0 29 122154.0 30 141314.0 31 159208.0 32 176142.5 33 192086.5 34 208562.5 35 223128.0 36 235044.0 37 245702.5 38 255100.0 39 260507.0 40 263623.5 41 263475.5 42 259297.0 43 256611.0 44 252703.5 45 248059.5 46 243390.0 47 235545.5 48 226944.5 49 221008.0 50 214794.5 51 205016.5 52 192796.5 53 181953.5 54 173529.0 55 163097.0 56 148732.5 57 133028.0 58 119366.5 59 107384.0 60 93848.5 61 79369.0 62 67127.0 63 55431.5 64 44479.0 65 35509.5 66 27938.5 67 21786.5 68 16790.0 69 12752.5 70 9499.0 71 7012.0 72 5291.0 73 3890.0 74 2711.0 75 1879.0 76 1231.0 77 801.0 78 518.0 79 356.0 80 258.5 81 160.5 82 112.0 83 91.0 84 72.0 85 59.5 86 47.0 87 35.5 88 25.0 89 21.0 90 23.5 91 20.0 92 18.5 93 18.5 94 21.0 95 17.0 96 10.5 97 12.0 98 12.5 99 18.0 100 32.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.02022340033523698 2 0.0028122519893581076 3 0.0017369791698976548 4 0.002371114422399973 5 0.00234354332446509 6 0.004645730002027854 7 11.61688911717758 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.9638930279999665E-4 18-19 0.0 20-21 1.3785548967441705E-5 22-23 0.0 24-25 0.0 26-27 2.0678323451162555E-4 28-29 0.0 30-31 0.0 32-33 5.514219586976682E-5 34-35 1.0339161725581277E-4 36-37 7.582051932092937E-5 38-39 6.892774483720852E-6 40-41 0.0 42-43 6.892774483720852E-6 44-45 1.86104911060463E-4 46-47 0.0012475921815534741 48-49 7.92669065627898E-4 50-51 4.2045924350697195E-4 52-53 2.6881820486511325E-4 54-55 4.8249421386045965E-4 56-57 0.001771443042316259 58-59 3.7910259660464685E-4 60-61 0.0013716621222604496 62-63 0.0023917927458511354 64-65 0.0011373077898139405 66-67 5.808954623900585 68-69 5.4601113072794725 70-71 0.0033223173011534507 72-73 0.0015991236802232378 74-75 0.0036531704763720515 76-77 0.006968595003041781 78-79 5.414357070256534 80-81 0.006100105418092954 82-83 0.005645182302167378 84-85 5.809126943262679 86-87 0.004645730002027854 88-89 0.002240151707209277 90-91 0.004149450239199953 92-93 0.004838727687572038 94-95 0.005279865254530173 96-97 0.003984023651590652 98-99 0.006947916679590619 100 0.00736148314861387 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7253973.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.47614327357915 #Duplication Level Percentage of deduplicated Percentage of total 1 90.36070341711965 77.23684431583114 2 6.738303155893479 11.51928331947923 3 1.4886929910273592 3.8174320617428292 4 0.5874606621073507 2.008554868875183 5 0.3052145584242053 1.3044281662524784 6 0.1602725212532816 0.8219686193677954 7 0.1071217096898504 0.6409445423612283 8 0.06640804359952615 0.4541042759384951 9 0.04329007001603415 0.3330241404312428 >10 0.14121331057601075 1.7693075624015016 >50 0.0010754646409484269 0.05837922550714792 >100 2.2713364275857867E-4 0.022955748340660005 >500 1.6962009556189727E-5 0.012773153471078014 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.757109793488341E-5 0.0 0.0 0.0 3 0.0 2.757109793488341E-5 0.0 1.3785548967441705E-5 0.0 4 0.0 2.757109793488341E-5 0.0 2.757109793488341E-5 0.0 5 0.0 2.757109793488341E-5 0.0 2.757109793488341E-5 0.0 6 0.0 2.757109793488341E-5 0.0 2.757109793488341E-5 0.0 7 0.0 2.757109793488341E-5 0.0 4.135664690232511E-5 0.0 8 0.0 2.757109793488341E-5 0.0 4.135664690232511E-5 0.0 9 0.0 2.757109793488341E-5 0.0 4.135664690232511E-5 0.0 10-11 0.0 2.757109793488341E-5 0.0 4.824942138604596E-5 0.0 12-13 0.0 6.203497035348768E-5 0.0 6.203497035348768E-5 0.0 14-15 0.0 1.3785548967441705E-4 0.0 6.892774483720852E-5 0.0 16-17 0.0 2.0678323451162555E-4 0.0 7.582051932092937E-5 0.0 18-19 0.0 2.2056878347906727E-4 0.0 1.0339161725581277E-4 0.0 20-21 0.0 2.2746155796278812E-4 0.0 1.86104911060463E-4 0.0 22-23 0.0 2.3435433244650897E-4 0.0 3.6531704763720515E-4 0.0 24-25 0.0 2.412471069302298E-4 0.0 7.650979676930145E-4 0.0 26-27 0.0 2.757109793488341E-4 0.0 0.001254484956037195 0.0 28-29 0.0 2.757109793488341E-4 0.0 0.0029638930279999665 0.0 30-31 0.0 2.757109793488341E-4 0.0 0.008347149899785952 0.0 32-33 0.0 2.757109793488341E-4 0.0 0.020692109000129997 0.0 34-35 0.0 2.757109793488341E-4 0.0 0.03999187755454839 0.0 36-37 0.0 3.308531752186009E-4 0.0 0.07011330204840852 0.0 38-39 0.0 3.9288814557208855E-4 0.0 0.11041535445472433 0.0 40-41 0.0 3.997809200558094E-4 0.0 0.1552873163437471 0.0 42-43 0.0 4.135664690232511E-4 0.0 0.20228914554823957 0.0 44-45 0.0 4.135664690232511E-4 0.0 0.2616979688234296 0.0 46-47 0.0 4.4113756695813455E-4 0.0 0.33649146474628455 0.0 48-49 0.0 4.756014393767388E-4 0.0 0.4166337536685069 0.0 50-51 0.0 4.8249421386045965E-4 0.0 0.49995361162772456 0.0 52-53 0.0 5.376364097302265E-4 0.0 0.5842108869167282 0.0 54-55 0.0 5.376364097302265E-4 0.0 0.681033690089555 0.0 56-57 0.0 5.445291842139473E-4 0.0 0.7864311047201307 0.0 58-59 0.0 5.514219586976682E-4 0.0 0.9060483131106223 0.0 60-61 0.0 5.514219586976682E-4 0.0 1.0325582959848347 0.0 62-63 0.0 5.514219586976682E-4 0.0 1.162507773326424 0.0 64-65 1.3785548967441705E-5 5.58314733181389E-4 0.0 1.3065529744872224 0.0 66-67 1.3785548967441705E-5 5.652075076651099E-4 0.0 1.4710076808943182 0.0 68-69 1.3785548967441705E-5 5.652075076651099E-4 0.0 1.6374681846761767 0.0 70-71 1.3785548967441705E-5 5.721002821488307E-4 0.0 1.842404155626165 0.0 72-73 1.3785548967441705E-5 6.134569290511558E-4 0.0 2.0684940514666925 0.0 74-75 1.3785548967441705E-5 6.203497035348767E-4 0.0 2.2755047475362815 0.0 76-77 1.3785548967441705E-5 6.54813575953481E-4 0.0 2.4967972723361394 0.0 78-79 1.3785548967441705E-5 6.685991249209226E-4 0.0 2.75775771428981 0.0 80-81 1.3785548967441705E-5 6.892774483720852E-4 0.0 3.0751079442948024 0.0 82-83 2.0678323451162556E-5 6.892774483720852E-4 0.0 3.411599409041087 0.0 84-85 2.757109793488341E-5 7.099557718232477E-4 0.0 3.748442405286041 0.0 86-87 2.757109793488341E-5 7.306340952744103E-4 0.0 4.148816103947451 0.0 88 2.757109793488341E-5 7.44419644241852E-4 0.0 4.4806480531427395 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7235 0.0 44.43503 1 GTATCAA 11750 0.0 34.418636 1 TCAACGC 12245 0.0 32.144314 4 ATCAACG 12355 0.0 32.023838 3 CAACGCA 13360 0.0 29.576315 5 TATCAAC 14150 0.0 28.142313 2 AACGCAG 14680 0.0 26.846924 6 GTACATG 21700 0.0 24.251453 1 ACGCAGA 16940 0.0 23.143461 7 TACATGG 21715 0.0 22.93452 2 CGCAGAG 17105 0.0 22.373562 8 GTGGTAT 4265 0.0 22.085293 1 ACATGGG 22540 0.0 21.095919 3 CATGGGG 15975 0.0 18.327019 4 TGGTATC 4730 0.0 18.179619 2 GCAGAGT 20120 0.0 18.054083 9 AGAGTAC 17220 0.0 16.64184 10-11 CAGAGTA 18880 0.0 16.448505 10-11 GAGTACA 16390 0.0 16.210365 1 TATACCG 485 1.364242E-10 15.830068 5 >>END_MODULE