FastQCFastQC Report
Wed 25 May 2016
SRR2049518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049518_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7253973
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307050.4232852810452975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292070.4026345286920699No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183940.25357138770712273No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80270.11065660156165456No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155150.059.786341
GTATCAA279500.040.363841
GTGGTAT59850.033.1998251
ATCAACG352300.031.9492153
TCAACGC356100.031.6743494
TATCAAC363000.030.9558242
CAACGCA370000.030.4605625
TGGTATC65100.029.4190122
AACGCAG386500.029.2572546
ACGCAGA439200.025.617187
CGCAGAG440650.025.4688918
GCAGAGT470600.023.3886139
GTACATG224200.023.0387291
TACATGG227750.021.79762
GAGTACT342750.021.64395312-13
CAGAGTA456350.021.3795610-11
ACATGGG227000.020.978113
AGAGTAC442700.020.54191610-11
AGTACTT355050.019.55061512-13
GTACTTT377500.019.52084414-15
Produced by FastQC (version 0.11.5)