##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049518_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7253973
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.811561746921306	31.0	31.0	34.0	30.0	34.0
2	32.0297951205498	33.0	31.0	34.0	30.0	34.0
3	32.04026317715823	34.0	31.0	34.0	30.0	34.0
4	35.6692776496411	37.0	35.0	37.0	33.0	37.0
5	35.77930452732592	37.0	35.0	37.0	35.0	37.0
6	35.83291583798285	37.0	35.0	37.0	35.0	37.0
7	35.82411018072441	37.0	35.0	37.0	35.0	37.0
8	35.814142815254485	37.0	35.0	37.0	35.0	37.0
9	37.56676541255392	39.0	37.0	39.0	35.0	39.0
10-11	37.52166150880352	39.0	37.0	39.0	35.0	39.0
12-13	37.499062858381194	39.0	37.0	39.0	35.0	39.0
14-15	38.77334151919231	40.0	38.0	41.0	35.0	41.0
16-17	38.78143949529451	40.0	38.0	41.0	35.0	41.0
18-19	38.858505401109156	40.0	38.0	41.0	35.0	41.0
20-21	38.88799572317129	40.0	38.0	41.0	35.0	41.0
22-23	38.857105202900534	40.0	38.0	41.0	35.0	41.0
24-25	38.81727620160704	40.0	38.0	41.0	35.0	41.0
26-27	38.72341715360672	40.0	38.0	41.0	34.5	41.0
28-29	38.63568026790284	40.0	38.0	41.0	34.0	41.0
30-31	38.51009805247414	40.0	38.0	41.0	34.0	41.0
32-33	38.350794109655496	40.0	38.0	41.0	34.0	41.0
34-35	38.245822117066055	40.0	38.0	41.0	34.0	41.0
36-37	38.107739234761425	40.0	38.0	41.0	34.0	41.0
38-39	38.019744421436364	40.0	38.0	41.0	33.0	41.0
40-41	37.898130927699896	40.0	37.0	41.0	33.0	41.0
42-43	37.75547041600513	40.0	37.0	41.0	33.0	41.0
44-45	37.55673794209049	40.0	37.0	41.0	33.0	41.0
46-47	37.29123109501511	40.0	37.0	41.0	32.0	41.0
48-49	37.044618583499	40.0	37.0	41.0	31.0	41.0
50-51	36.831785836533996	40.0	36.5	41.0	31.0	41.0
52-53	36.73687846646244	40.0	36.0	41.0	31.0	41.0
54-55	36.611305694134785	40.0	36.0	41.0	31.0	41.0
56-57	36.44355810257358	39.0	35.5	41.0	31.0	41.0
58-59	36.27511468542825	39.0	35.0	41.0	30.0	41.0
60-61	36.09089708494918	39.0	35.0	41.0	30.0	41.0
62-63	35.89547459026936	39.0	35.0	41.0	30.0	41.0
64-65	35.62392395174341	38.0	35.0	40.0	29.0	41.0
66-67	35.37506667036119	37.5	35.0	40.0	29.0	41.0
68-69	35.07402729511124	37.0	35.0	40.0	29.0	41.0
70-71	34.785389882206616	37.0	34.0	39.5	29.0	41.0
72-73	34.467640078064804	36.0	34.0	39.0	28.0	41.0
74-75	34.129454782916895	36.0	34.0	39.0	28.0	41.0
76-77	33.83645906870621	35.0	34.0	38.5	28.0	40.0
78-79	33.50775237238959	35.0	34.0	37.0	28.0	39.0
80-81	33.19917243970993	35.0	34.0	37.0	27.5	39.0
82-83	32.90339893738231	35.0	34.0	36.5	27.0	39.0
84-85	32.6277751516307	35.0	33.5	36.0	27.0	37.0
86-87	32.35210683855592	35.0	33.0	36.0	26.0	37.0
88-89	32.14282063084602	35.0	33.0	35.0	26.0	37.0
90-91	31.99047535743516	35.0	33.0	35.0	26.0	36.0
92-93	31.834520613738153	35.0	33.0	35.0	26.0	36.0
94-95	31.655730590670796	35.0	33.0	35.0	25.5	36.0
96-97	31.492033193396225	35.0	33.0	35.0	25.0	36.0
98-99	31.31895183508403	35.0	33.0	35.0	25.0	35.0
100	31.21045556690106	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.43163798080047044
1101	2	0.4351153002794206
1101	3	0.49987374273210605
1101	4	0.381478412819078
1101	5	0.3304249511884265
1101	6	0.314892314524549
1101	7	0.3156139239686411
1101	8	0.3397173277746077
1101	9	0.3631481049150693
1101	10-11	0.3578146534518325
1101	12-13	0.36561315213127443
1101	14-15	0.481051730945083
1101	16-17	0.4924782472969227
1101	18-19	0.4301874934118928
1101	20-21	0.4381169554040625
1101	22-23	0.45487892876003144
1101	24-25	0.5082666080502278
1101	26-27	0.4497749035238954
1101	28-29	0.4880056907340773
1101	30-31	0.4707715736217253
1101	32-33	0.4762269338596212
1101	34-35	0.4707212273272603
1101	36-37	0.4776188883665853
1101	38-39	0.48060895017033545
1101	40-41	0.49041675282818886
1101	42-43	0.44699576097021776
1101	44-45	0.5019215602387845
1101	46-47	0.5658806366515989
1101	48-49	0.5421973947828036
1101	50-51	0.5694588268029293
1101	52-53	0.49101931738944415
1101	54-55	0.46316791431200954
1101	56-57	0.4943835966717316
1101	58-59	0.4971376671668182
1101	60-61	0.5544133224762149
1101	62-63	0.46442863400464063
1101	64-65	0.457292416640783
1101	66-67	0.4947613098973207
1101	68-69	0.498866634447495
1101	70-71	0.5109059779931542
1101	72-73	0.5054403555555567
1101	74-75	0.49752298049090626
1101	76-77	0.4271994504013321
1101	78-79	0.3929117117499814
1101	80-81	0.3937452784958495
1101	82-83	0.4272871894119774
1101	84-85	0.4419264046483917
1101	86-87	0.505374067502629
1101	88-89	0.5181419966940197
1101	90-91	0.5123999984886929
1101	92-93	0.5168573628754132
1101	94-95	0.4625265979506139
1101	96-97	0.4748761670940489
1101	98-99	0.5195264312599797
1101	100	0.5280144768318458
1102	1	0.27864077373415697
1102	2	0.32559285446034636
1102	3	0.38680522829820774
1102	4	0.351571270077649
1102	5	0.28514498131421107
1102	6	0.24839309849662072
1102	7	0.2796257334548855
1102	8	0.26961169154247244
1102	9	0.3469568673633958
1102	10-11	0.2752363103594817
1102	12-13	0.2694432709902159
1102	14-15	0.4057291579271407
1102	16-17	0.38342138053633334
1102	18-19	0.38220402625238137
1102	20-21	0.35874182288110745
1102	22-23	0.41164766894227256
1102	24-25	0.3746411267460701
1102	26-27	0.35013143167257965
1102	28-29	0.4109633194771618
1102	30-31	0.48182141766244513
1102	32-33	0.4755308968842442
1102	34-35	0.46125502115676653
1102	36-37	0.44361841910908595
1102	38-39	0.45865030291332687
1102	40-41	0.4631052015108139
1102	42-43	0.4858952808743382
1102	44-45	0.5357972182386135
1102	46-47	0.5750963175914094
1102	48-49	0.5768361513705287
1102	50-51	0.6067023660981832
1102	52-53	0.5596774037097703
1102	54-55	0.5327560001841576
1102	56-57	0.47614936465163993
1102	58-59	0.4920205262862467
1102	60-61	0.47059579741350177
1102	62-63	0.4829043110010858
1102	64-65	0.4995614543135929
1102	66-67	0.5452656506836959
1102	68-69	0.5125995174439879
1102	70-71	0.5546914664124571
1102	72-73	0.5886447148422889
1102	74-75	0.5926036993659096
1102	76-77	0.5722559436791386
1102	78-79	0.4735460356288428
1102	80-81	0.4457170675504116
1102	82-83	0.4811813807853511
1102	84-85	0.4521738988795576
1102	86-87	0.44956452657576307
1102	88-89	0.4606144339947491
1102	90-91	0.4593248643997434
1102	92-93	0.5349986538340517
1102	94-95	0.546832434085438
1102	96-97	0.5377285579583209
1102	98-99	0.5565105533370627
1102	100	0.6227169724603385
1103	1	0.3277012806230424
1103	2	0.368144264536717
1103	3	0.4267838481845416
1103	4	0.33203787332691803
1103	5	0.24142561531247964
1103	6	0.29394324906898106
1103	7	0.2962682523719167
1103	8	0.3138549885399158
1103	9	0.3564405124844612
1103	10-11	0.3144291735914777
1103	12-13	0.3091414199867515
1103	14-15	0.41807646350572014
1103	16-17	0.4104750655826095
1103	18-19	0.4189889138152054
1103	20-21	0.4129287242072053
1103	22-23	0.4173536317450015
1103	24-25	0.4678604304447518
1103	26-27	0.4719596005037303
1103	28-29	0.4633216223859904
1103	30-31	0.4808674996682356
1103	32-33	0.4823268552395277
1103	34-35	0.4572634687143662
1103	36-37	0.5049060537390773
1103	38-39	0.5155168549105724
1103	40-41	0.5336016831836616
1103	42-43	0.5671649165168802
1103	44-45	0.5811137114353357
1103	46-47	0.5149277977037485
1103	48-49	0.5312632489548434
1103	50-51	0.571728415257418
1103	52-53	0.5161109705553599
1103	54-55	0.49910077526925534
1103	56-57	0.5074443689956993
1103	58-59	0.4804372976194671
1103	60-61	0.4922383054230792
1103	62-63	0.49899116473896044
1103	64-65	0.5355545161993405
1103	66-67	0.5642616405978984
1103	68-69	0.5196665168633956
1103	70-71	0.5433838981658283
1103	72-73	0.5360143034298588
1103	74-75	0.48958293325834745
1103	76-77	0.4521922942670571
1103	78-79	0.47628299241797123
1103	80-81	0.44750723814625104
1103	82-83	0.46818997569619825
1103	84-85	0.4999859117670624
1103	86-87	0.5301348215490975
1103	88-89	0.5391263653671743
1103	90-91	0.47987567547942334
1103	92-93	0.5298734247237817
1103	94-95	0.5313556569725648
1103	96-97	0.534620537762482
1103	98-99	0.577552885339383
1103	100	0.5417453295635752
1104	1	0.2887836518698883
1104	2	0.2643368359748095
1104	3	0.3531069032827574
1104	4	0.285171320481453
1104	5	0.18727608734572243
1104	6	0.21695452259788794
1104	7	0.16714102628112926
1104	8	0.20431046618691084
1104	9	0.23641121603096593
1104	10-11	0.21673560217326582
1104	12-13	0.23640647301426299
1104	14-15	0.3106810342754258
1104	16-17	0.32727665688258156
1104	18-19	0.2824145983766897
1104	20-21	0.28428333684050244
1104	22-23	0.31361754590377444
1104	24-25	0.3046134384891843
1104	26-27	0.3426011978499588
1104	28-29	0.3034800968928977
1104	30-31	0.35342138997327055
1104	32-33	0.3279703375666472
1104	34-35	0.24740226957280242
1104	36-37	0.30183354385255257
1104	38-39	0.3332652080820253
1104	40-41	0.35461467101536925
1104	42-43	0.33147232439325336
1104	44-45	0.3353104310850341
1104	46-47	0.32063132898484525
1104	48-49	0.307778375098124
1104	50-51	0.34524112952063746
1104	52-53	0.33263798148072965
1104	54-55	0.2854701516459386
1104	56-57	0.3366035905102365
1104	58-59	0.28550397400506
1104	60-61	0.2524420158892937
1104	62-63	0.2682098835931228
1104	64-65	0.29548292990867253
1104	66-67	0.2807855263055572
1104	68-69	0.24343709292966764
1104	70-71	0.25606672116334295
1104	72-73	0.27608511065478325
1104	74-75	0.3249746358679957
1104	76-77	0.27441291457889605
1104	78-79	0.21834282609907518
1104	80-81	0.2396547301545624
1104	82-83	0.2802914455156369
1104	84-85	0.28397291408510483
1104	86-87	0.26965043250408627
1104	88-89	0.25078571090538304
1104	90-91	0.2383675725689507
1104	92-93	0.22772311375118903
1104	94-95	0.20803160198377668
1104	96-97	0.2258489113006803
1104	98-99	0.3002506699809011
1104	100	0.3749880285983025
1105	1	0.3058137694246419
1105	2	0.30484213292587015
1105	3	0.3780698615922091
1105	4	0.3193562601228379
1105	5	0.2425490847118823
1105	6	0.2525618546089561
1105	7	0.24373033596589977
1105	8	0.23563767198913155
1105	9	0.26706049586015723
1105	10-11	0.26160342092519073
1105	12-13	0.2696953392244197
1105	14-15	0.35454512684908934
1105	16-17	0.3519294778418285
1105	18-19	0.3626549345233485
1105	20-21	0.2939375958763435
1105	22-23	0.3108495796688757
1105	24-25	0.33959408535442037
1105	26-27	0.3472071242640311
1105	28-29	0.3708808664027714
1105	30-31	0.3463771224286347
1105	32-33	0.32687611726698407
1105	34-35	0.2917120804176534
1105	36-37	0.30206767468803264
1105	38-39	0.3210255095609966
1105	40-41	0.3094873360640378
1105	42-43	0.3049877400187526
1105	44-45	0.36421869803820783
1105	46-47	0.4094144811376239
1105	48-49	0.43339560483694584
1105	50-51	0.4763759792234765
1105	52-53	0.4035434697194802
1105	54-55	0.42474299772558766
1105	56-57	0.37812863975314315
1105	58-59	0.4049404659358018
1105	60-61	0.3774029655925162
1105	62-63	0.3948234033934952
1105	64-65	0.42306654241690467
1105	66-67	0.40322926503117884
1105	68-69	0.4429084234945435
1105	70-71	0.4320872747608675
1105	72-73	0.47412272372969966
1105	74-75	0.46229919800251906
1105	76-77	0.43024094967907445
1105	78-79	0.382891780747741
1105	80-81	0.3974719905780617
1105	82-83	0.3989555215076237
1105	84-85	0.4146663191470452
1105	86-87	0.47151164123204126
1105	88-89	0.42614464242943484
1105	90-91	0.38305252268073176
1105	92-93	0.3588765964820908
1105	94-95	0.43513932439098113
1105	96-97	0.4240402895934601
1105	98-99	0.45317652548689225
1105	100	0.43771919460277076
1106	1	0.19606332638968738
1106	2	0.18326170753883275
1106	3	0.27586593365514744
1106	4	0.21277823718748579
1106	5	0.17728237175158768
1106	6	0.19212172219447865
1106	7	0.15857871207593632
1106	8	0.18192799674262972
1106	9	0.19505336484468927
1106	10-11	0.18453079095295521
1106	12-13	0.1826938071256663
1106	14-15	0.24209383415863073
1106	16-17	0.2702895973888033
1106	18-19	0.25450923532009995
1106	20-21	0.20619720272339492
1106	22-23	0.21144833512950356
1106	24-25	0.24193613895460686
1106	26-27	0.2799840154956357
1106	28-29	0.2921732425275039
1106	30-31	0.3136757951404192
1106	32-33	0.2989484226939112
1106	34-35	0.25917063761580295
1106	36-37	0.28359647491957674
1106	38-39	0.2984832870339602
1106	40-41	0.2985844102167192
1106	42-43	0.30213019009490694
1106	44-45	0.28240148113458474
1106	46-47	0.29783680724155204
1106	48-49	0.29045942513927514
1106	50-51	0.27293085942543627
1106	52-53	0.26694198562893945
1106	54-55	0.21907511003377067
1106	56-57	0.25728583030940655
1106	58-59	0.24664020968789657
1106	60-61	0.2262242129813572
1106	62-63	0.21317782808898045
1106	64-65	0.21239859553129747
1106	66-67	0.2570689421024852
1106	68-69	0.25224369348561737
1106	70-71	0.19921504069308327
1106	72-73	0.19854045067973658
1106	74-75	0.2313778077729296
1106	76-77	0.2107973591221608
1106	78-79	0.23208109108286124
1106	80-81	0.15349951120300886
1106	82-83	0.16900464419432382
1106	84-85	0.1926884788551817
1106	86-87	0.23210026842991738
1106	88-89	0.24153358037433037
1106	90-91	0.20534411050738655
1106	92-93	0.16794175905479136
1106	94-95	0.17633747215583995
1106	96-97	0.2906074902698208
1106	98-99	0.27061575284863437
1106	100	0.3515319242083663
1107	1	0.13778938213627967
1107	2	0.182168626800582
1107	3	0.24903350306130534
1107	4	0.2382207610744942
1107	5	0.18582153255577794
1107	6	0.17566254707129758
1107	7	0.15838614145157948
1107	8	0.1689456505626552
1107	9	0.13022993330081079
1107	10-11	0.146861051564251
1107	12-13	0.13958575896979397
1107	14-15	0.18308866136101187
1107	16-17	0.16900396195033096
1107	18-19	0.18775016086932794
1107	20-21	0.20491416291972797
1107	22-23	0.18258213707842685
1107	24-25	0.22796179847674125
1107	26-27	0.2159268694900618
1107	28-29	0.2619671475310952
1107	30-31	0.2778157227874942
1107	32-33	0.29972463693346896
1107	34-35	0.25377612819556106
1107	36-37	0.28687704680003634
1107	38-39	0.27891122918602207
1107	40-41	0.28724514068250073
1107	42-43	0.3139629964984536
1107	44-45	0.36544010640994884
1107	46-47	0.34271939378049865
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2216	100	-1.4015674244376086
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	525.0
3	58.0
4	68.0
5	166.0
6	479.0
7	1321.0
8	2716.0
9	4346.0
10	6090.0
11	8514.0
12	12327.0
13	17951.0
14	20663.0
15	26422.0
16	35197.0
17	50111.0
18	52491.0
19	28468.0
20	20160.0
21	19593.0
22	20032.0
23	21949.0
24	25085.0
25	30696.0
26	39005.0
27	50138.0
28	65670.0
29	86045.0
30	111992.0
31	144361.0
32	186417.0
33	242471.0
34	330320.0
35	482614.0
36	776978.0
37	1430994.0
38	2050286.0
39	848389.0
40	2865.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.655311895046324	20.501393057362954	15.14855738074869	22.694737666842034
2	15.37709163541589	20.884350918733553	40.16574135509287	23.572816090757687
3	17.790057944797972	25.800068459036176	31.42683326778305	24.983040328382806
4	12.607682321176334	17.775343353640242	38.106891861787894	31.510082463395534
5	12.481389126617906	37.67508006811304	35.52185716113322	14.321673644135839
6	29.649808011529373	38.41045981145661	17.625428033032524	14.314304143981497
7	25.79556058452382	33.54185354701485	22.644956081308823	18.017629787152504
8	24.68303921175334	36.22953931590316	20.444451612929907	18.642969859413594
9	24.693698507464333	16.854453647688352	22.027184033447092	36.42466381140022
10-11	23.58254573045695	27.299357193637196	28.67354207135869	20.444555004547162
12-13	24.441750748176204	24.899107013494536	29.224240288735565	21.43490194959369
14-15	21.62303091485816	26.753861660471095	27.32758186325932	24.295525561411427
16-17	20.325179043263603	29.99971188202658	28.084595848371645	21.590513226338174
18-19	20.20214991150367	28.069852203750965	31.348283209766564	20.379714674978803
20-21	21.187216991295664	27.21525845216132	31.358643049815598	20.238881506727417
22-23	20.95428946316729	27.064148157154705	31.460304580675995	20.52125779900201
24-25	20.810623364603096	27.12906430724239	31.39285878235279	20.667453545801727
26-27	20.562007881749768	27.212825302768568	31.395167861804836	20.829998953676835
28-29	20.59903811192848	27.239994692555964	31.56769145139639	20.593275744119165
30-31	20.877075006837654	27.44040076361134	31.155168934120326	20.52735529543068
32-33	20.618524582118596	27.314628454590117	31.43376483945623	20.633082123835052
34-35	20.806790981990144	27.132696799395312	31.33934328126118	20.721168937353365
36-37	21.078490366589453	27.22641096127598	30.948550539132142	20.746548133002424
38-39	20.910099885952153	27.38524116370436	31.028189655517053	20.676469294826436
40-41	20.83002652477477	27.261136759124966	31.045993692008505	20.862843024091763
42-43	20.844694348876125	27.347703113866018	30.852444584505623	20.955157952752238
44-45	21.016586358951155	27.499364830831325	30.572784045377617	20.911264764839903
46-47	21.154979485035305	27.628700851243863	30.13953181242886	21.076787851291975
48-49	21.230048691937508	27.67984523791307	29.80733454618593	21.28277152396349
50-51	21.266594182250195	27.76533631983466	29.59940711111001	21.368662386805134
52-53	21.223004276415143	27.933623408854707	29.51145530869773	21.33191700603242
54-55	21.247473625832356	28.132445488837632	29.401784373887246	21.218296511442762
56-57	21.17092247241615	27.90421194013267	29.44041148209402	21.484454105357162
58-59	21.06723667272405	27.649870594784254	29.724668932464184	21.558223800027516
60-61	21.23125492747216	27.821657180141145	29.536083191928064	21.41100470045863
62-63	21.064380857221277	27.823662977515905	29.65874700664036	21.453209158622457
64-65	21.052311609100283	27.679355850924726	29.726951010156778	21.541381529818214
66-67	20.98519666395229	27.637020430045716	29.658347225720306	21.719435680281688
68-69	21.239224438747183	27.820466647757485	29.406859482855772	21.533449430639557
70-71	21.188898828269696	27.64695981085124	29.42657768370519	21.737563677173874
72-73	21.064932279179974	27.753584139339914	29.51754852134134	21.663935060138765
74-75	21.118764847897832	27.833898747624232	29.289632040262624	21.757704364215307
76-77	21.186651783788	27.782582041592928	29.17668014479789	21.854086029821175
78-79	21.199644856222395	27.805943605864574	29.29650114726935	21.697910390643678
80-81	21.094163944758645	27.741887367702244	29.303788232879207	21.86016045465991
82-83	20.984088533269663	27.77235467953947	29.383993633262754	21.859563153928114
84-85	21.001263721273844	27.691907593259586	29.18730191027731	22.119526775189264
86-87	21.089314929806733	27.7144232153088	29.1746163343338	22.021645520550674
88-89	20.99064408546082	27.625495978580002	29.221760367688443	22.162099568270744
90-91	20.8873129851138	27.550149104600408	29.18355857097798	22.37897933930781
92-93	20.88113335701708	27.66175773399179	29.128240886064482	22.328868022926642
94-95	20.82203196480797	27.467794976919357	29.231104858548797	22.479068199723873
96-97	20.783625799518816	27.520603365948798	29.17926694649036	22.516503888042035
98-99	20.765354748183046	27.520745710331422	29.103017320378864	22.610882221106664
100	20.79514632503824	27.435064107026786	29.073110478040388	22.696679089894587
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1418.0
1	1259.5
2	3107.0
3	4883.5
4	3001.5
5	1481.0
6	3089.0
7	8068.5
8	13346.5
9	15020.0
10	11371.5
11	6907.5
12	6066.0
13	6528.5
14	7324.5
15	8109.5
16	8970.5
17	9944.0
18	11183.0
19	12564.5
20	14367.0
21	16937.5
22	20733.5
23	27228.0
24	35423.0
25	45033.0
26	58363.0
27	75015.0
28	95342.5
29	117279.0
30	135590.0
31	152219.0
32	168559.0
33	184248.5
34	200002.5
35	215028.0
36	227514.5
37	238196.5
38	248049.5
39	254263.0
40	257852.5
41	258390.5
42	255299.5
43	252978.5
44	249881.5
45	246536.5
46	242948.5
47	234629.5
48	224626.5
49	219261.0
50	214084.0
51	204213.0
52	191445.5
53	181414.0
54	174270.5
55	163135.5
56	148929.0
57	133792.0
58	120768.5
59	110431.5
60	96911.0
61	80895.5
62	68370.5
63	56762.5
64	45358.5
65	36361.0
66	28718.0
67	22079.5
68	16769.0
69	12799.0
70	9360.5
71	6691.5
72	5057.5
73	3628.0
74	2534.5
75	1751.0
76	1052.5
77	634.5
78	417.5
79	277.5
80	161.0
81	72.0
82	34.0
83	25.0
84	11.5
85	2.5
86	3.0
87	3.5
88	1.0
89	2.5
90	2.5
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.5
98	0.5
99	1.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06135947845408303
2	2.757109793488341E-4
3	0.0
4	0.12157475634386838
5	0.002054046796148814
6	0.0020954034430511388
7	0.0
8	0.0
9	1.2406994070697532E-4
10-11	0.0
12-13	0.0
14-15	2.0678323451162556E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.447482641581379E-4
30-31	2.894965283162758E-4
32-33	1.3785548967441705E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.0678323451162555E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	6.892774483720852E-6
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.3096271519069617E-4
80-81	3.2396040073488005E-4
82-83	3.032820772837175E-4
84-85	0.0
86-87	1.3785548967441705E-5
88-89	7.582051932092937E-5
90-91	6.892774483720852E-5
92-93	7.44419644241852E-4
94-95	7.995618401116189E-4
96-97	6.892774483720852E-6
98-99	4.824942138604596E-5
100	0.00121312830913487
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7253973.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.73280216489075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.27523194429723	38.83550739484515
2	15.828524950281306	17.010219994310052
3	5.12696727228019	8.264589544419032
4	2.2789267596374003	4.898124828954879
5	1.2151839473331296	3.2647619318001038
6	0.7445773053928251	2.400493502828407
7	0.5049723818933851	1.8993506766507648
8	0.3307343808498038	1.421702804426411
9	0.25648146222935536	1.2403320902038666
>10	1.32851995210146	12.747025097244805
>50	0.07629633151510733	2.780439712450131
>100	0.03186586058866833	3.0154425352259784
>500	0.00105101992578608	0.3974877769377788
>1k	4.8700776860700223E-4	0.4031156465382785
>5k	1.0252794611671178E-4	0.34291479128587504
>10k+	7.689595958753382E-5	1.0784916718785338
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30705	0.4232852810452975	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29207	0.4026345286920699	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18394	0.25357138770712273	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8027	0.11065660156165456	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3785548967441705E-5	0.0
5	0.0	1.3785548967441705E-5	0.0	1.3785548967441705E-5	0.0
6	0.0	2.757109793488341E-5	0.0	1.3785548967441705E-5	0.0
7	0.0	2.757109793488341E-5	0.0	1.3785548967441705E-5	0.0
8	0.0	4.135664690232511E-5	0.0	1.3785548967441705E-5	0.0
9	0.0	4.135664690232511E-5	0.0	1.3785548967441705E-5	0.0
10-11	0.0	6.203497035348768E-5	0.0	1.3785548967441705E-5	0.0
12-13	0.0	7.582051932092937E-5	0.0	1.3785548967441705E-5	0.0
14-15	0.0	1.309627151906962E-4	0.0	1.3785548967441705E-5	0.0
16-17	0.0	1.9299768554418385E-4	0.0	1.3785548967441705E-5	0.0
18-19	0.0	2.0678323451162555E-4	0.0	3.446387241860426E-5	0.0
20-21	0.0	2.0678323451162555E-4	0.0	1.1717716622325449E-4	0.0
22-23	6.892774483720852E-6	2.0678323451162555E-4	0.0	2.9638930279999665E-4	0.0
24-25	1.3785548967441705E-5	2.4124710693022985E-4	0.0	6.754918994046435E-4	0.0
26-27	1.3785548967441705E-5	2.619254303813924E-4	0.0	0.0011717716622325447	0.0
28-29	1.3785548967441705E-5	2.894965283162758E-4	0.0	0.002922536381097641	0.0
30-31	1.3785548967441705E-5	2.894965283162758E-4	0.0	0.008312686027367348	0.0
32-33	1.3785548967441705E-5	2.894965283162758E-4	0.0	0.020774822293934646	0.0
34-35	1.3785548967441705E-5	2.9638930279999665E-4	0.0	0.04015730414215768	0.0
36-37	2.0678323451162556E-5	3.2396040073488005E-4	0.0	0.07045104799811083	0.0
38-39	2.757109793488341E-5	3.446387241860426E-4	0.0	0.11102191860929177	0.0
40-41	2.757109793488341E-5	3.72209822120926E-4	0.0	0.15613512760524473	0.0
42-43	2.757109793488341E-5	3.859953710883677E-4	0.0	0.20330238339734652	0.0
44-45	2.757109793488341E-5	3.859953710883677E-4	0.0	0.2631661297884621	0.0
46-47	2.757109793488341E-5	3.859953710883677E-4	0.0	0.33813194507341016	0.0
48-49	2.757109793488341E-5	3.859953710883677E-4	0.0	0.4183500545149534	0.0
50-51	2.757109793488341E-5	3.997809200558094E-4	0.0	0.5018008751893617	0.0
52-53	2.757109793488341E-5	4.135664690232511E-4	0.0	0.5863131831342632	0.0
54-55	2.757109793488341E-5	4.135664690232511E-4	0.0	0.6870372966648759	0.0
56-57	2.757109793488341E-5	4.135664690232511E-4	0.0	0.8074609596699629	0.0
58-59	2.757109793488341E-5	4.2735201799069285E-4	0.0	0.944551351376687	0.0
60-61	2.757109793488341E-5	4.2735201799069285E-4	0.0	1.0890859395258294	1.3785548967441705E-5
62-63	2.757109793488341E-5	4.2735201799069285E-4	0.0	1.2380801527659395	1.3785548967441705E-5
64-65	2.757109793488341E-5	4.2735201799069285E-4	0.0	1.4031758872000213	1.3785548967441705E-5
66-67	2.757109793488341E-5	4.342447924744137E-4	0.0	1.600081775876475	1.3785548967441705E-5
68-69	2.757109793488341E-5	4.4113756695813455E-4	0.0	1.8208435570410864	1.3785548967441705E-5
70-71	2.757109793488341E-5	4.5492311592557625E-4	0.0	2.0622491977844417	1.3785548967441705E-5
72-73	2.757109793488341E-5	5.307436352465057E-4	0.0	2.3206455276301687	1.3785548967441705E-5
74-75	2.757109793488341E-5	5.58314733181389E-4	0.0	2.585024785727766	1.3785548967441705E-5
76-77	2.757109793488341E-5	5.652075076651099E-4	0.0	2.8781262350990278	1.3785548967441705E-5
78-79	2.757109793488341E-5	5.721002821488307E-4	0.0	3.2108128883302984	1.3785548967441705E-5
80-81	3.446387241860426E-5	5.789930566325516E-4	0.0	3.575599192332257	1.3785548967441705E-5
82-83	4.824942138604596E-5	5.927786055999933E-4	0.0	3.9604158989839084	1.3785548967441705E-5
84-85	5.514219586976682E-5	6.203497035348767E-4	0.0	4.347328009078611	1.3785548967441705E-5
86-87	5.514219586976682E-5	6.479208014697601E-4	0.0	4.7631897720049405	1.3785548967441705E-5
88	5.514219586976682E-5	6.754918994046435E-4	0.0	5.097703010474398	1.3785548967441705E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15515	0.0	59.78634	1
GTATCAA	27950	0.0	40.36384	1
GTGGTAT	5985	0.0	33.199825	1
ATCAACG	35230	0.0	31.949215	3
TCAACGC	35610	0.0	31.674349	4
TATCAAC	36300	0.0	30.955824	2
CAACGCA	37000	0.0	30.460562	5
TGGTATC	6510	0.0	29.419012	2
AACGCAG	38650	0.0	29.257254	6
ACGCAGA	43920	0.0	25.61718	7
CGCAGAG	44065	0.0	25.468891	8
GCAGAGT	47060	0.0	23.388613	9
GTACATG	22420	0.0	23.038729	1
TACATGG	22775	0.0	21.7976	2
GAGTACT	34275	0.0	21.643953	12-13
CAGAGTA	45635	0.0	21.37956	10-11
ACATGGG	22700	0.0	20.97811	3
AGAGTAC	44270	0.0	20.541916	10-11
AGTACTT	35505	0.0	19.550615	12-13
GTACTTT	37750	0.0	19.520844	14-15
>>END_MODULE