FastQCFastQC Report
Wed 25 May 2016
SRR2049517_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049517_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701591
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29700.039.1484531
GTATCAA45900.035.9325831
TACCTGG22300.033.9875682
ATCAACG48100.032.6849253
TCAACGC48250.032.5833134
GTACCTG23650.031.403791
CAACGCA53200.029.4553365
TATCAAC54600.029.4510172
AACGCAG57700.026.9806236
GTCTCGC9800.026.1329921
TCTGTCG10950.026.0207528
GTACATG71100.024.3496271
GTGGTAT18500.023.8106881
ACGCAGA66100.023.6288997
TACATGG73200.022.9470062
CTGTCGC13550.022.6967189
CGCAGAG65350.022.284548
ATAACGC1853.417792E-622.1448353
TCGCTCT11300.021.7528934
TGGTATC19250.021.5485252
Produced by FastQC (version 0.11.5)