##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049517_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2701591 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.34039016268562 31.0 31.0 34.0 27.0 34.0 2 30.562517420290487 31.0 31.0 34.0 27.0 34.0 3 30.60742503213847 31.0 31.0 34.0 27.0 34.0 4 34.17788221829285 37.0 35.0 37.0 30.0 37.0 5 34.21816403741351 37.0 35.0 37.0 30.0 37.0 6 34.14624863645163 37.0 35.0 37.0 30.0 37.0 7 30.2951664408121 37.0 32.0 37.0 2.0 37.0 8 31.999045747487315 37.0 32.0 37.0 17.0 37.0 9 34.65644614599323 38.0 33.0 39.0 27.0 39.0 10-11 35.20643816921214 38.5 35.0 39.0 28.5 39.0 12-13 35.35803809681036 39.0 35.0 39.0 29.0 39.0 14-15 36.488533238376945 40.0 37.0 41.0 29.0 41.0 16-17 36.48254065844904 40.0 37.0 41.0 29.0 41.0 18-19 36.47059973178767 40.0 37.0 41.0 29.0 41.0 20-21 36.554923746784766 40.0 37.0 41.0 29.0 41.0 22-23 36.50323494563018 40.0 37.0 41.0 29.0 41.0 24-25 36.48080186823246 40.0 36.5 41.0 29.0 41.0 26-27 36.514838108359115 40.0 37.0 41.0 30.0 41.0 28-29 36.49836485241474 40.0 36.5 41.0 30.0 41.0 30-31 36.420035083030704 40.0 36.0 41.0 30.0 41.0 32-33 36.36813307417741 40.0 36.0 41.0 30.0 41.0 34-35 36.307122543715906 40.0 36.0 41.0 29.5 41.0 36-37 36.128553322838286 40.0 36.0 41.0 29.0 41.0 38-39 35.993486060621315 40.0 36.0 41.0 28.0 41.0 40-41 35.91004763489366 40.0 35.5 41.0 28.0 41.0 42-43 35.77095681766781 40.0 35.0 41.0 27.5 41.0 44-45 35.619722230345005 39.0 35.0 41.0 27.0 41.0 46-47 35.44978440481923 39.0 35.0 41.0 27.0 41.0 48-49 35.358433049266154 39.0 35.0 41.0 27.0 41.0 50-51 35.22459043578395 39.0 35.0 41.0 26.0 41.0 52-53 35.083738989358494 39.0 35.0 41.0 26.0 41.0 54-55 34.94095516308724 39.0 34.0 40.0 26.0 41.0 56-57 34.73853148015373 39.0 34.0 40.0 25.0 41.0 58-59 34.55469462253909 38.0 34.0 40.0 25.0 41.0 60-61 34.309922190294536 38.0 34.0 40.0 24.5 41.0 62-63 34.02939082932983 37.5 33.5 40.0 23.5 41.0 64-65 33.6419237775074 37.0 33.0 40.0 22.5 41.0 66-67 30.26267373558766 36.0 29.0 39.0 6.5 41.0 68-69 30.562732663826612 35.0 28.5 39.0 7.0 41.0 70-71 30.684014900849164 35.0 28.0 39.0 11.0 40.5 72-73 31.836128784853074 35.0 30.5 38.0 17.5 40.0 74-75 31.920473528376426 35.0 32.0 37.5 18.0 40.0 76-77 31.631465680778476 35.0 32.0 37.0 15.0 39.0 78-79 28.390503225691823 35.0 27.0 36.0 4.5 39.0 80-81 30.085186654826728 34.0 28.5 36.0 7.0 38.5 82-83 30.484799327507382 35.0 31.0 36.0 4.5 37.0 84-85 28.734936561455825 34.5 28.5 35.0 2.0 37.0 86-87 28.36748937940643 34.0 26.0 35.0 2.0 36.5 88-89 29.54725585775197 34.0 30.0 35.0 2.0 36.0 90-91 29.695096148898926 34.0 30.5 35.0 2.0 36.0 92-93 29.54173059504566 34.0 31.0 35.0 2.0 36.0 94-95 29.32528165810443 34.0 30.5 35.0 2.0 35.5 96-97 29.124403175758285 34.0 30.0 35.0 2.0 35.0 98-99 28.923953144646987 34.0 30.0 35.0 2.0 35.0 100 28.712114083886124 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2843438083804237 1101 2 0.23453989825220845 1101 3 0.2515256646457118 1101 4 0.29582807592215943 1101 5 0.3308127721260803 1101 6 0.2452878584132563 1101 7 -11.936778898880863 1101 8 -6.419633758317239 1101 9 -2.2663926546100086 1101 10-11 -0.2924329849926437 1101 12-13 0.21669773306507523 1101 14-15 0.373255681587608 1101 16-17 0.3321585490302539 1101 18-19 0.38527712513561596 1101 20-21 0.3870098531136321 1101 22-23 0.26313325448956704 1101 24-25 0.37484584534116294 1101 26-27 0.239300662934113 1101 28-29 0.24763088605971006 1101 30-31 0.29082997081974327 1101 32-33 0.2951789571784076 1101 34-35 0.28473960262240894 1101 36-37 0.3150359388460231 1101 38-39 0.355563243388886 1101 40-41 0.3235956758751186 1101 42-43 0.41146491694985343 1101 44-45 0.2203344321283609 1101 46-47 0.1698685138362208 1101 48-49 0.12637081382554527 1101 50-51 0.19280889762184472 1101 52-53 0.2161808013552502 1101 54-55 0.22044377875810284 1101 56-57 0.20903633209128003 1101 58-59 0.11825133080028394 1101 60-61 0.20300363095490326 1101 62-63 0.1858815821826525 1101 64-65 0.05850730389797576 1101 66-67 -10.036775239833908 1101 68-69 -8.058875918958822 1101 70-71 -6.727049831501201 1101 72-73 -1.8459578281934235 1101 74-75 -0.6133126712665025 1101 76-77 -0.3448509158301931 1101 78-79 2.6664791903517404 1101 80-81 0.6339887120360714 1101 82-83 -0.02528942333001183 1101 84-85 -5.4460370278348655 1101 86-87 -5.92896120547114 1101 88-89 -1.6573649317404993 1101 90-91 -0.33770693549786657 1101 92-93 -0.025165468664436474 1101 94-95 0.015193637275107363 1101 96-97 0.15491465287108852 1101 98-99 0.14147222501125256 1101 100 0.2138882028283433 1102 1 0.09591314843239829 1102 2 0.21854443979123772 1102 3 0.16505589608738802 1102 4 0.13583869811857596 1102 5 0.07817733680225558 1102 6 0.08101288694036413 1102 7 -12.068565642713754 1102 8 -6.552578684733945 1102 9 -2.6151856091274155 1102 10-11 -0.5938032801409889 1102 12-13 -0.02106152427718655 1102 14-15 0.12220628572337233 1102 16-17 0.19601920977752485 1102 18-19 0.16079021208188493 1102 20-21 0.15938704606237764 1102 22-23 0.1700346866304443 1102 24-25 0.1965409326349672 1102 26-27 0.2082974088095284 1102 28-29 0.21459408572930272 1102 30-31 0.17922540017372057 1102 32-33 0.19778372810068845 1102 34-35 0.24577016509646654 1102 36-37 0.35095941449655044 1102 38-39 0.30490309389611525 1102 40-41 0.22844232139370746 1102 42-43 0.15272400509778095 1102 44-45 0.1557070685604245 1102 46-47 0.22964295585610728 1102 48-49 0.08421587714849466 1102 50-51 0.17483914079379304 1102 52-53 0.1657096617462699 1102 54-55 0.14953498450994118 1102 56-57 0.1741193738355591 1102 58-59 0.11987050663501009 1102 60-61 0.05847452601744152 1102 62-63 0.05778374531195851 1102 64-65 -0.15622204017808627 1102 66-67 -10.151730726415728 1102 68-69 -6.8040309170044395 1102 70-71 -6.2982191439306945 1102 72-73 -1.5332680147525402 1102 74-75 -0.6644364649201968 1102 76-77 -0.37570286926589347 1102 78-79 2.669985449614188 1102 80-81 0.6832868571015567 1102 82-83 0.00535290939657429 1102 84-85 -5.604947518400348 1102 86-87 -6.1140998328219816 1102 88-89 -1.7978739979190088 1102 90-91 -0.5477833360498394 1102 92-93 -0.1813958560724025 1102 94-95 -0.1857695973864928 1102 96-97 -0.008353138479495925 1102 98-99 -0.04343706548726445 1102 100 0.027527704780670348 1103 1 0.08007151643225896 1103 2 0.13028133915118545 1103 3 0.14147286841075157 1103 4 0.14223011798692653 1103 5 0.17722371333189102 1103 6 0.17395471266613782 1103 7 -12.161530611245261 1103 8 -6.685171130388085 1103 9 -2.4758980896483536 1103 10-11 -0.441192371105565 1103 12-13 0.1974050995371499 1103 14-15 0.320776454519617 1103 16-17 0.12679742476782963 1103 18-19 0.2650862225546433 1103 20-21 0.20646107061536156 1103 22-23 0.270097248500889 1103 24-25 0.3436526914055449 1103 26-27 0.3018596397505675 1103 28-29 0.21773505402683213 1103 30-31 0.19637701670505692 1103 32-33 0.21508310254556307 1103 34-35 0.20900349480578484 1103 36-37 0.16468367050939037 1103 38-39 0.2244593369540695 1103 40-41 0.1709601696468397 1103 42-43 0.08611891482494372 1103 44-45 0.13206545270939074 1103 46-47 0.18220618053905469 1103 48-49 0.1176051919116361 1103 50-51 0.12724313152904188 1103 52-53 0.09252403546316401 1103 54-55 0.0880512254825021 1103 56-57 0.08673210759220495 1103 58-59 0.03251626975313826 1103 60-61 0.08125954016061598 1103 62-63 -0.01358475745659149 1103 64-65 -0.13652649523177018 1103 66-67 -10.317548325502223 1103 68-69 -8.344159556671588 1103 70-71 -6.896034628375105 1103 72-73 -1.8506038200427035 1103 74-75 -0.6379123299620346 1103 76-77 -0.33248495113929266 1103 78-79 2.696099213034948 1103 80-81 0.6524744047461724 1103 82-83 -0.055457991627871905 1103 84-85 -5.631566672378206 1103 86-87 -6.240943770837415 1103 88-89 -1.8050680090186582 1103 90-91 -0.5008944221531557 1103 92-93 -0.13158808759201435 1103 94-95 -0.06492632186566993 1103 96-97 -0.03704484515333206 1103 98-99 -0.023026668518209448 1103 100 0.06830971544543374 1104 1 0.10271313182969521 1104 2 0.1455425404816495 1104 3 0.08559194346310761 1104 4 0.1055097545007655 1104 5 0.13990514843555246 1104 6 0.186500456414187 1104 7 -11.979449116720016 1104 8 -6.533507326102487 1104 9 -2.527218770532727 1104 10-11 -0.5707454592161838 1104 12-13 0.012772553177441637 1104 14-15 0.19239241356221015 1104 16-17 0.29377795695331343 1104 18-19 0.3000924183415066 1104 20-21 0.3733396078299407 1104 22-23 0.22596730364701756 1104 24-25 0.30980014388639887 1104 26-27 0.278700259474185 1104 28-29 0.33262795024921843 1104 30-31 0.270785699502504 1104 32-33 0.2255435654541742 1104 34-35 0.1707688773865641 1104 36-37 0.28665280751961575 1104 38-39 0.2336246630118879 1104 40-41 0.2180932594966194 1104 42-43 0.19631986122232803 1104 44-45 0.1567490509035494 1104 46-47 0.12701222704731663 1104 48-49 0.06213315266440844 1104 50-51 0.12007277637639646 1104 52-53 0.04051112938549295 1104 54-55 -0.002674388117789306 1104 56-57 0.023203462041941236 1104 58-59 0.0033281352284717514 1104 60-61 -0.03649845457839973 1104 62-63 -0.11576460556923251 1104 64-65 -0.23493262000259563 1104 66-67 -10.240641218825992 1104 68-69 -8.347827118285249 1104 70-71 -6.895308344470383 1104 72-73 -2.069041720866636 1104 74-75 -0.8722413371143638 1104 76-77 -0.5856303999533985 1104 78-79 2.4332785538901867 1104 80-81 0.4200398228349691 1104 82-83 -0.15980219838599297 1104 84-85 -5.6158299172313555 1104 86-87 -6.173209780243415 1104 88-89 -1.9100597842021756 1104 90-91 -0.6754084514436478 1104 92-93 -0.4130503657574778 1104 94-95 -0.3105648140068027 1104 96-97 -0.22861685396512144 1104 98-99 -0.22999828746644724 1104 100 -0.20810245293503726 1105 1 -0.02599987671760573 1105 2 0.015147845934290416 1105 3 0.006209879066073398 1105 4 0.05073101240283506 1105 5 0.019925769472763477 1105 6 0.058662949059602454 1105 7 -12.080294823615564 1105 8 -6.640978739555223 1105 9 -2.6645836388268904 1105 10-11 -0.5903353270874021 1105 12-13 -0.01095867500552572 1105 14-15 0.12075040473106924 1105 16-17 0.15934517175985974 1105 18-19 0.08083012325953831 1105 20-21 0.04074913467394481 1105 22-23 0.07321748739418865 1105 24-25 0.11709095878392617 1105 26-27 0.09883457818106223 1105 28-29 0.09830543685118442 1105 30-31 0.1670881520337062 1105 32-33 0.15100319183180488 1105 34-35 0.13704152152235594 1105 36-37 0.2675005727593529 1105 38-39 0.21138314968716543 1105 40-41 0.24919610903447875 1105 42-43 0.15063895344554368 1105 44-45 0.1475314989609089 1105 46-47 0.14340519972867583 1105 48-49 0.08007787249462694 1105 50-51 0.2039720368763014 1105 52-53 0.12282468945624458 1105 54-55 0.14907527493408423 1105 56-57 0.07654547519654642 1105 58-59 0.17889009138856693 1105 60-61 0.012740381019106906 1105 62-63 -0.06499458276766035 1105 64-65 -0.2333080319063967 1105 66-67 -10.082979807986128 1105 68-69 -8.343208649768517 1105 70-71 -6.830989083068189 1105 72-73 -2.0887560057753056 1105 74-75 -0.8861725207468645 1105 76-77 -0.5557939255304376 1105 78-79 2.497052330585216 1105 80-81 0.4707192643939706 1105 82-83 -0.13261525467935442 1105 84-85 -5.504529889598789 1105 86-87 -6.043377723271455 1105 88-89 -1.8217065347440204 1105 90-91 -0.5424343649631176 1105 92-93 -0.1566401274219018 1105 94-95 -0.2046333991630398 1105 96-97 -0.040264144939797575 1105 98-99 -0.061540085951730106 1105 100 -0.04736221973121957 1106 1 0.02389784173952947 1106 2 0.14791065157209715 1106 3 0.11597512560377865 1106 4 0.09746106977363667 1106 5 0.05080514085869936 1106 6 0.0468705857028624 1106 7 -11.965418850634702 1106 8 -6.575774113998808 1106 9 -2.581059202000084 1106 10-11 -0.45963873923601284 1106 12-13 0.15032937957795411 1106 14-15 0.31074759922955764 1106 16-17 0.36120790549959736 1106 18-19 0.32971550432502283 1106 20-21 0.35330253290863567 1106 22-23 0.4215424212604475 1106 24-25 0.46551309135124797 1106 26-27 0.3516755176038018 1106 28-29 0.2987606209522795 1106 30-31 0.25769341342807905 1106 32-33 0.2692806934134495 1106 34-35 0.26535581833532973 1106 36-37 0.3486319849488595 1106 38-39 0.272101216094633 1106 40-41 0.18523675132394857 1106 42-43 0.2764044464584856 1106 44-45 0.15472232871920966 1106 46-47 0.17857409205534225 1106 48-49 0.11398448791261728 1106 50-51 0.16581639287740302 1106 52-53 0.11094194238186361 1106 54-55 0.07520076944631171 1106 56-57 0.14430870109166705 1106 58-59 -0.004838851732444027 1106 60-61 -0.010485394838354978 1106 62-63 -0.0943931434431633 1106 64-65 -0.19484176664226283 1106 66-67 -10.06331701904503 1106 68-69 -8.108988942653788 1106 70-71 -6.674201813911555 1106 72-73 -1.795785774758727 1106 74-75 -0.5669699135613726 1106 76-77 -0.30658875136785824 1106 78-79 2.6718546392744322 1106 80-81 0.5861886804521177 1106 82-83 -0.034594609822907074 1106 84-85 -5.563124463963323 1106 86-87 -6.057284009298581 1106 88-89 -1.7983187610514442 1106 90-91 -0.5507575894043732 1106 92-93 -0.18566176272261004 1106 94-95 -0.11428900965493227 1106 96-97 0.030473906406555784 1106 98-99 0.037424945232498175 1106 100 0.09034394771519416 1107 1 -0.058655844326665374 1107 2 -0.07560635825525708 1107 3 -0.02517568569071571 1107 4 -0.05022450388250377 1107 5 -0.0635417506370004 1107 6 -0.1633612748086435 1107 7 -12.134294471460805 1107 8 -6.799537559891522 1107 9 -2.8219954473240385 1107 10-11 -0.735083249055009 1107 12-13 -0.11622785900090804 1107 14-15 -0.11073398687597091 1107 16-17 0.07256992860429534 1107 18-19 0.06227311843611005 1107 20-21 0.014097246038787148 1107 22-23 0.08226528738487104 1107 24-25 0.1408273070850541 1107 26-27 0.071497880827053 1107 28-29 0.020217970185001377 1107 30-31 0.07709299644243117 1107 32-33 0.006903813941470105 1107 34-35 0.003455012333944296 1107 36-37 0.1068612111630145 1107 38-39 0.12823437068799137 1107 40-41 0.08468509781205569 1107 42-43 -0.022298797270856596 1107 44-45 0.03161430915587715 1107 46-47 0.008880713568991894 1107 48-49 -0.029969805245457337 1107 50-51 0.02028216205545874 1107 52-53 0.028460214755284596 1107 54-55 -0.053704626517323106 1107 56-57 -0.10162145436976999 1107 58-59 -0.06892479289757603 1107 60-61 -0.09757346550527046 1107 62-63 -0.1653277841189933 1107 64-65 -0.272573401500118 1107 66-67 -10.303869792467601 1107 68-69 -8.417387631968278 1107 70-71 -6.93813570352291 1107 72-73 -2.149307921688777 1107 74-75 -0.9049106206986011 1107 76-77 -0.5746298715905453 1107 78-79 2.4777879371717866 1107 80-81 0.4392035802786154 1107 82-83 -0.24203719629283071 1107 84-85 -5.780514531637127 1107 86-87 -6.302470454924968 1107 88-89 -1.9800763912090211 1107 90-91 -0.6999338700305877 1107 92-93 -0.30090235408001575 1107 94-95 -0.3038883546464817 1107 96-97 -0.29720939399805957 1107 98-99 -0.1977844056316158 1107 100 -0.13499105044057558 1108 1 -0.1099304098680669 1108 2 -0.1429867874786197 1108 3 -0.11829448051123848 1108 4 -0.14813809288258284 1108 5 -0.17804960568356876 1108 6 -0.09837412692479575 1108 7 -12.061728416412919 1108 8 -6.6731035776232694 1108 9 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-0.21452680786622835 2214 64-65 -0.13190469672489513 2214 66-67 3.000068388743557 2214 68-69 2.2547882904742274 2214 70-71 1.8339820606674806 2214 72-73 0.36784403437326674 2214 74-75 0.15827552435700554 2214 76-77 0.08027239212022153 2214 78-79 2.9708103859692976 2214 80-81 1.0015544071372418 2214 82-83 0.289919159731884 2214 84-85 1.6980944253504369 2214 86-87 1.894826841261093 2214 88-89 0.5866000712890802 2214 90-91 0.21587943889642602 2214 92-93 0.1604880906980135 2214 94-95 0.27177066942145345 2214 96-97 0.23154841234421397 2214 98-99 0.1944801048420075 2214 100 0.13163986606687317 2215 1 -0.4002070426094768 2215 2 -0.5840763127146467 2215 3 -0.4808052254635662 2215 4 -0.49973124218026754 2215 5 -0.41093810747258885 2215 6 -0.4360785396219953 2215 7 3.297667300915446 2215 8 1.3474945606424171 2215 9 0.12698025802395563 2215 10-11 -0.37361119745834515 2215 12-13 -0.805409190936146 2215 14-15 -0.9429394375770102 2215 16-17 -0.9111808427432067 2215 18-19 -0.8988598776905974 2215 20-21 -0.9422765412637517 2215 22-23 -0.8462350169362338 2215 24-25 -0.7609636532072628 2215 26-27 -0.8248220481773458 2215 28-29 -0.6646487190333374 2215 30-31 -0.7468575597080829 2215 32-33 -0.7210828351562597 2215 34-35 -0.6294895506099039 2215 36-37 -0.660948849443912 2215 38-39 -0.7893076848861398 2215 40-41 -0.741956264298004 2215 42-43 -0.6937828972838815 2215 44-45 -0.676816703590184 2215 46-47 -0.591013269646318 2215 48-49 -0.558428521124263 2215 50-51 -0.5930174506152639 2215 52-53 -0.5089265189534089 2215 54-55 -0.5144848484713833 2215 56-57 -0.33752314114806126 2215 58-59 -0.3792751164733801 2215 60-61 -0.47647480725977687 2215 62-63 -0.5045553441807513 2215 64-65 -0.4442189941586179 2215 66-67 2.620113357464639 2215 68-69 2.0742442230070033 2215 70-71 1.669500278240605 2215 72-73 0.07372570632074016 2215 74-75 -0.212448557498508 2215 76-77 -0.272155306550399 2215 78-79 2.607141911757285 2215 80-81 0.5269806063338152 2215 82-83 -0.20643368303956677 2215 84-85 1.4161073248186362 2215 86-87 1.6628943022866842 2215 88-89 0.314451343768841 2215 90-91 -0.11971889658856227 2215 92-93 -0.4233336034499686 2215 94-95 -0.3461951571406061 2215 96-97 -0.33794360715612015 2215 98-99 -0.4659896302534925 2215 100 -0.37695764808842114 2216 1 -0.38491257137377843 2216 2 -0.23391041982134553 2216 3 -0.30745611853392774 2216 4 -0.44747168926479475 2216 5 -0.12646505122064156 2216 6 -0.35650363675605234 2216 7 3.5430131971320513 2216 8 1.816331190192777 2216 9 0.6899802861721724 2216 10-11 0.00207406413395006 2216 12-13 -0.3465073505874017 2216 14-15 -0.4637470440431031 2216 16-17 -0.5488878343069103 2216 18-19 -1.0606509625138898 2216 20-21 -0.6942924872300722 2216 22-23 -0.5384357128328503 2216 24-25 -0.5392500599780945 2216 26-27 -0.8499068219882346 2216 28-29 -0.9549537345012382 2216 30-31 -0.7617882038573853 2216 32-33 -1.6938113532977894 2216 34-35 -0.840915366885568 2216 36-37 -0.6038069992160473 2216 38-39 -0.6894834864066937 2216 40-41 -0.7977057424209377 2216 42-43 -0.4239862431991739 2216 44-45 -0.47221285799859913 2216 46-47 -0.6493350750731111 2216 48-49 -0.6964561526876807 2216 50-51 -1.2867644258166848 2216 52-53 -0.9246738473695828 2216 54-55 -0.49333895261798233 2216 56-57 -0.6066465079838324 2216 58-59 -0.8906189056595579 2216 60-61 -0.9481508884465057 2216 62-63 -0.7031821764255426 2216 64-65 -0.40027252980198824 2216 66-67 2.7497054897649065 2216 68-69 1.9401999617479397 2216 70-71 1.569639418975342 2216 72-73 0.06260273310604347 2216 74-75 -0.7503863985119175 2216 76-77 -0.49475636319823835 2216 78-79 2.014328833186525 2216 80-81 0.6498327723393018 2216 82-83 -0.3379044819859196 2216 84-85 1.4253640597341395 2216 86-87 1.392046459756905 2216 88-89 0.23162628631468962 2216 90-91 -0.10891304484614395 2216 92-93 -0.11375267595705907 2216 94-95 -0.06377612640184438 2216 96-97 -0.023970252169839057 2216 98-99 -0.03894902054490146 2216 100 -1.0958320734334421 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63520.0 3 10579.0 4 6279.0 5 5512.0 6 5181.0 7 5468.0 8 5677.0 9 6408.0 10 7195.0 11 8077.0 12 9186.0 13 10170.0 14 11543.0 15 12450.0 16 13288.0 17 13766.0 18 12445.0 19 12058.0 20 11550.0 21 11716.0 22 12521.0 23 14756.0 24 17915.0 25 21892.0 26 26929.0 27 32142.0 28 39202.0 29 48918.0 30 59594.0 31 71944.0 32 95286.0 33 131686.0 34 198535.0 35 256666.0 36 299128.0 37 491631.0 38 497105.0 39 143394.0 40 279.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.53903529182664 19.72724516281658 12.760138713497838 22.97358083185894 2 17.314636350142383 20.721210597951316 38.557882334563864 23.406270717342434 3 18.289139813404454 25.479305513655977 30.276302809667733 25.955251863271844 4 13.263168648801113 17.993084708069336 38.00460402532029 30.73914261780926 5 12.551248243604796 36.28279406386542 33.90853271225916 17.25742498027063 6 29.022962126114017 37.886397590664714 18.646029675453416 14.444610607767855 7 26.346942000031014 32.346347713148035 22.464253727809062 18.842456559011893 8 24.56242435010494 34.584189310739234 21.753893077780123 19.09949326137571 9 24.07085306399081 18.279561932209575 22.492597880286098 35.15698712351352 10-11 23.136015037065196 27.06473703828596 28.573736735131263 21.225511189517583 12-13 24.233442441879617 24.844619337272 28.310928634275136 22.611009586573243 14-15 22.06348037138116 27.447752083864657 25.9077151204605 24.581052424293684 16-17 21.123739906854713 30.365052232281336 26.02297317205523 22.488234688808724 18-19 20.743461908186696 28.846649992541433 28.37727842593494 22.032609673336935 20-21 22.57456809709538 28.857995159148814 27.307038704230212 21.2603980395256 22-23 22.025706333786278 29.21654314069006 27.3387052296221 21.41904529590156 24-25 21.6888862895975 28.71382085593267 27.780759559829743 21.816533294640085 26-27 21.447952897306823 28.388023111604976 27.94794836293415 22.21607562815405 28-29 21.65033493226769 28.589116561315166 27.78858828001722 21.971960226399926 30-31 22.183224625785325 29.549421063365994 26.96983000017397 21.29752431067471 32-33 21.147242495255572 28.86541671185609 28.0310565144761 21.956284278412237 34-35 21.223846636895296 27.960753460417827 28.190673746205242 22.62472615648164 36-37 22.41179779096795 28.956109373348777 27.091522567909532 21.54057026777374 38-39 22.025584933317045 28.88994999241188 27.47715234361987 21.60731273065121 40-41 21.35184415405589 28.557653619663377 27.94573641976154 22.144765806519196 42-43 21.202869643963304 28.60503048298048 27.84168357183799 22.350416301218225 44-45 21.889625657206057 28.860766334466987 27.528087184278284 21.72152082404867 46-47 21.542094188686452 29.674024311450413 27.02959560684514 21.754285893017997 48-49 21.302970046350758 28.687516980841536 27.970379489882564 22.039133482925138 50-51 21.382771535857792 28.2290761911125 27.999506955462937 22.388645317566766 52-53 21.426230209060723 28.51938501993368 27.71924317719589 22.335141593809706 54-55 21.786341168013656 29.34812542891599 27.32682528507413 21.538708117996222 56-57 21.131907852728716 29.00593919405377 27.333488799927448 22.528664153290062 58-59 20.923645536728078 28.20241136891316 28.124271837472737 22.74967125688602 60-61 21.863324976393155 29.009323176683584 27.1512026257548 21.976149221168466 62-63 21.55792068716934 28.88200798255055 27.288144533581587 22.271926796698523 64-65 21.460252865058127 28.42780253405487 27.813416658045103 22.298527942841904 66-67 21.412734672505152 28.53050010397785 27.28284451950243 22.773920704014568 68-69 22.38001443921168 28.854592987132964 27.005163770698115 21.760228802957236 70-71 22.260095801214977 28.31730816390629 27.340232753162418 22.082363281716315 72-73 21.572646915115712 28.587186662520914 28.0007847317846 21.839381690578776 74-75 21.499260691653543 28.487940917519694 27.800066740415502 22.21273165041126 76-77 21.68787111015113 28.75027508492235 27.214038002345788 22.347815802580737 78-79 22.00842476999024 28.983647381708078 27.246730914850637 21.76119693345105 80-81 21.552051545155315 28.521038128785893 27.629618031031928 22.297292295026867 82-83 21.360994538905256 28.57690970814811 27.7636681913904 22.298427561556235 84-85 21.672022146290683 28.579332906677067 27.455664962662336 22.292979984369914 86-87 21.997110867008267 28.65403604105493 27.395199523228275 21.953653568708525 88-89 21.411279799967055 28.565122679345784 27.601307395185366 22.422290125501796 90-91 21.297317846407438 28.353293507377092 27.580242786935948 22.769145859279526 92-93 21.72854774330844 28.826273857587953 27.120720755402832 22.324457643700775 94-95 21.685066742032667 28.427991509109717 27.464465357645164 22.42247639121246 96-97 21.44102345706226 28.442643476677215 27.640573671655172 22.47575939460535 98-99 21.514460472253703 28.349936450806705 27.615923371536883 22.51967970540271 100 21.920473466353847 28.618017515116023 27.192110276082595 22.269398742447542 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3352.0 1 2514.5 2 2025.0 3 2297.5 4 2039.0 5 1735.0 6 1518.5 7 1430.0 8 1409.0 9 1380.5 10 1249.0 11 1090.0 12 1077.5 13 1095.0 14 1130.0 15 1231.5 16 1361.5 17 1574.5 18 1883.0 19 2333.0 20 3095.5 21 4056.0 22 5629.0 23 8318.5 24 11323.5 25 14827.5 26 19977.5 27 26393.5 28 34283.0 29 41959.0 30 47676.0 31 53636.0 32 60067.5 33 66035.0 34 71800.0 35 77115.5 36 81062.5 37 84377.5 38 87538.5 39 88921.0 40 89453.0 41 88825.5 42 86816.0 43 85934.5 44 85268.0 45 84680.0 46 85311.5 47 86160.0 48 87096.5 49 88256.0 50 87879.5 51 84461.0 52 79506.5 53 75903.5 54 73874.0 55 71098.5 56 66335.5 57 61312.0 58 56782.0 59 51529.0 60 44638.5 61 36865.5 62 29808.5 63 23268.5 64 17760.0 65 13727.0 66 10239.5 67 7402.5 68 5454.5 69 4044.0 70 2958.0 71 2188.5 72 1707.0 73 1253.0 74 907.5 75 666.0 76 481.0 77 336.0 78 239.5 79 193.5 80 160.5 81 119.0 82 87.5 83 71.5 84 61.5 85 63.5 86 53.0 87 38.0 88 27.0 89 19.5 90 15.0 91 13.5 92 14.5 93 10.0 94 8.0 95 6.5 96 4.5 97 7.5 98 9.0 99 9.0 100 17.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.01791536912878374 2 0.0018507612736346842 3 9.623958622900357E-4 4 0.0015546394698531345 5 0.0012955328915442788 6 0.0036645073217966744 7 11.67971021520282 8 3.701522547269368E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.850761273634684E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.665685146271216E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 9.25380636817342E-5 36-37 1.850761273634684E-5 38-39 3.701522547269368E-5 40-41 0.0 42-43 5.552283820904052E-5 44-45 1.1104567641808104E-4 46-47 9.068730240809953E-4 48-49 6.292588330357926E-4 50-51 3.5164464199059E-4 52-53 2.776141910452026E-4 54-55 4.8119793114501786E-4 56-57 0.0017027003717439094 58-59 4.997055438813648E-4 60-61 0.0016286699207985222 62-63 0.0027206190722429855 64-65 0.001165979602389851 66-67 5.840262275081609 68-69 5.482972811206434 70-71 0.002202405915625274 72-73 0.0011474719896535043 74-75 0.002942710425079148 76-77 0.005644821884585786 78-79 5.436148550983476 80-81 0.004256750929359773 82-83 0.004367796605777855 84-85 5.840225259856137 86-87 0.003275847454333391 88-89 0.0013325481170169727 90-91 0.0032018170033880035 92-93 0.003405400743487819 94-95 0.00397913673831457 96-97 0.0029797256505518415 98-99 0.005200639178913463 100 0.005663329497322134 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2701591.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.08047417303078 #Duplication Level Percentage of deduplicated Percentage of total 1 95.77599325294452 90.10650859630017 2 3.2481008604048762 6.111657382174401 3 0.5887030063343976 1.6615637394908664 4 0.18371568055002008 0.6913623335666774 5 0.08440860853390646 0.39706009575778256 6 0.040610088277855914 0.2292369816833594 7 0.02546148569056539 0.1676800052842761 8 0.014681386028814952 0.1104985407286576 9 0.008773255850401935 0.07428528634024174 >10 0.029013061235384774 0.40163045585043733 >50 4.981238774930608E-4 0.028908069313933308 >100 0.0 0.0 >500 4.11902717574572E-5 0.01960851350918331 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.701522547269368E-5 0.0 9 0.0 0.0 0.0 7.403045094538736E-5 0.0 10-11 0.0 0.0 0.0 7.403045094538736E-5 0.0 12-13 0.0 0.0 0.0 7.403045094538736E-5 0.0 14-15 0.0 0.0 0.0 7.403045094538736E-5 0.0 16-17 1.850761273634684E-5 1.850761273634684E-5 0.0 1.1104567641808104E-4 0.0 18-19 5.552283820904052E-5 3.701522547269368E-5 0.0 1.4806090189077472E-4 0.0 20-21 7.403045094538736E-5 3.701522547269368E-5 0.0 2.5910657830885577E-4 0.0 22-23 7.403045094538736E-5 3.701522547269368E-5 0.0 6.662740585084863E-4 0.0 24-25 7.403045094538736E-5 3.701522547269368E-5 0.0 0.0011474719896535043 0.0 26-27 7.403045094538736E-5 3.701522547269368E-5 0.0 0.0021283754646798866 0.0 28-29 7.403045094538736E-5 3.701522547269368E-5 0.0 0.004497349894932282 0.0 30-31 1.1104567641808104E-4 3.701522547269368E-5 0.0 0.011604273185689469 0.0 32-33 1.1104567641808104E-4 3.701522547269368E-5 0.0 0.026873053693175615 0.0 34-35 1.1104567641808104E-4 3.701522547269368E-5 0.0 0.050026077226345514 0.0 36-37 1.1104567641808104E-4 3.701522547269368E-5 0.0 0.08306216596072463 0.0 38-39 1.1104567641808104E-4 3.701522547269368E-5 0.0 0.1292756749633827 0.0 40-41 1.1104567641808104E-4 5.552283820904052E-5 0.0 0.1923866343943254 0.0 42-43 1.1104567641808104E-4 7.403045094538736E-5 0.0 0.2586068727649744 0.0 44-45 1.1104567641808104E-4 7.403045094538736E-5 0.0 0.33237821713205296 0.0 46-47 1.1104567641808104E-4 7.403045094538736E-5 0.0 0.41804995648860244 0.0 48-49 1.1104567641808104E-4 7.403045094538736E-5 0.0 0.5140304361392971 0.0 50-51 1.1104567641808104E-4 7.403045094538736E-5 0.0 0.6130276566660164 0.0 52-53 1.1104567641808104E-4 7.403045094538736E-5 0.0 0.7081197709053665 0.0 54-55 1.1104567641808104E-4 7.403045094538736E-5 0.0 0.8112812042977637 0.0 56-57 1.1104567641808104E-4 7.403045094538736E-5 0.0 0.9201429824129559 0.0 58-59 1.1104567641808104E-4 7.403045094538736E-5 0.0 1.0414973991251824 0.0 60-61 1.1104567641808104E-4 7.403045094538736E-5 0.0 1.1637586888614895 0.0 62-63 1.1104567641808104E-4 7.403045094538736E-5 0.0 1.286741775494514 0.0 64-65 1.1104567641808104E-4 7.403045094538736E-5 0.0 1.4204777851273565 0.0 66-67 1.1104567641808104E-4 7.403045094538736E-5 0.0 1.5731285749767452 0.0 68-69 1.4806090189077472E-4 7.403045094538736E-5 0.0 1.7175434771584595 0.0 70-71 1.4806090189077472E-4 7.403045094538736E-5 0.0 1.8911263770126565 0.0 72-73 1.4806090189077472E-4 7.403045094538736E-5 0.0 2.0941919039558545 0.0 74-75 1.4806090189077472E-4 7.403045094538736E-5 0.0 2.2765474122470795 0.0 76-77 1.4806090189077472E-4 7.403045094538736E-5 0.0 2.453665266133919 0.0 78-79 1.4806090189077472E-4 7.403045094538736E-5 0.0 2.661265158197521 0.0 80-81 1.4806090189077472E-4 7.403045094538736E-5 0.0 2.9081752197131245 0.0 82-83 1.4806090189077472E-4 7.403045094538736E-5 0.0 3.170243016059796 0.0 84-85 1.4806090189077472E-4 7.403045094538736E-5 0.0 3.4303860206818872 0.0 86-87 1.4806090189077472E-4 7.403045094538736E-5 0.0 3.7337072858178755 0.0 88 1.4806090189077472E-4 1.1104567641808104E-4 0.0 3.990241305956379 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2970 0.0 39.148453 1 GTATCAA 4590 0.0 35.932583 1 TACCTGG 2230 0.0 33.987568 2 ATCAACG 4810 0.0 32.684925 3 TCAACGC 4825 0.0 32.583313 4 GTACCTG 2365 0.0 31.40379 1 CAACGCA 5320 0.0 29.455336 5 TATCAAC 5460 0.0 29.451017 2 AACGCAG 5770 0.0 26.980623 6 GTCTCGC 980 0.0 26.132992 1 TCTGTCG 1095 0.0 26.020752 8 GTACATG 7110 0.0 24.349627 1 GTGGTAT 1850 0.0 23.810688 1 ACGCAGA 6610 0.0 23.628899 7 TACATGG 7320 0.0 22.947006 2 CTGTCGC 1355 0.0 22.696718 9 CGCAGAG 6535 0.0 22.28454 8 ATAACGC 185 3.417792E-6 22.144835 3 TCGCTCT 1130 0.0 21.752893 4 TGGTATC 1925 0.0 21.548525 2 >>END_MODULE