FastQCFastQC Report
Wed 25 May 2016
SRR2049517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049517_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701591
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127890.4733877185702795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122820.4546209992556238No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78980.2923462507833347No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75810.2806124243084908No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50840.18818540630317468No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36130.13373600963284227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68000.059.4215241
ATAACGC4150.039.690583
TATAACG4050.038.346562
GTATCAA118850.037.9208561
GTGGTAT25850.036.0719871
TGGTATC26450.034.5178342
TACCTGG22950.030.9643362
ATCAACG150700.029.9171183
TATCAAC150850.029.7937772
TCAACGC152250.029.5816354
GTACCTG24900.029.5046631
CAACGCA156800.028.8931715
TAACGCA6000.028.2370134
AACGCAG165450.027.6382356
CGCAGAG184300.024.5054288
ACGCAGA185450.024.4801757
TCTGTCG9900.023.2608268
GCAGAGT192900.023.169289
GTATAAC8500.022.1618751
GTACATG71350.022.0453971
Produced by FastQC (version 0.11.5)