##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049517_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2701591 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.645629556805602 31.0 31.0 34.0 30.0 34.0 2 31.907322018765978 33.0 31.0 34.0 30.0 34.0 3 31.883774783081524 33.0 31.0 34.0 30.0 34.0 4 35.563438359100246 37.0 35.0 37.0 33.0 37.0 5 35.70854433554154 37.0 35.0 37.0 33.0 37.0 6 35.79741641129246 37.0 35.0 37.0 35.0 37.0 7 35.78879149360507 37.0 35.0 37.0 33.0 37.0 8 35.77720313696633 37.0 35.0 37.0 33.0 37.0 9 37.49471811240117 39.0 37.0 39.0 35.0 39.0 10-11 37.451305360433906 39.0 37.0 39.0 34.5 39.0 12-13 37.44073973447498 39.0 37.0 39.0 34.0 39.0 14-15 38.679455180299314 40.0 38.0 41.0 34.0 41.0 16-17 38.679322480715996 40.0 38.0 41.0 34.0 41.0 18-19 38.77047321374701 40.0 38.0 41.0 34.5 41.0 20-21 38.78979571667214 40.0 38.0 41.0 35.0 41.0 22-23 38.726237983469744 40.0 38.0 41.0 34.0 41.0 24-25 38.67112212766477 40.0 38.0 41.0 34.0 41.0 26-27 38.55093128456528 40.0 38.0 41.0 34.0 41.0 28-29 38.44382125199559 40.0 38.0 41.0 34.0 41.0 30-31 38.27812037425354 40.0 38.0 41.0 34.0 41.0 32-33 38.05757163093895 40.0 37.0 41.0 33.5 41.0 34-35 37.891232980862014 40.0 37.0 41.0 33.0 41.0 36-37 37.703503602136664 40.0 37.0 41.0 33.0 41.0 38-39 37.57852613515517 40.0 37.0 41.0 33.0 41.0 40-41 37.4399836984947 40.0 37.0 41.0 33.0 41.0 42-43 37.27354233116708 40.0 37.0 41.0 32.5 41.0 44-45 37.04096845155318 40.0 37.0 41.0 31.5 41.0 46-47 36.71863394570089 40.0 36.0 41.0 31.0 41.0 48-49 36.4220823951516 40.0 36.0 41.0 30.0 41.0 50-51 36.1888687073654 40.0 36.0 41.0 30.0 41.0 52-53 36.06555803598694 39.5 35.0 41.0 29.0 41.0 54-55 35.92123197034636 39.0 35.0 41.0 29.0 41.0 56-57 35.74553531604155 39.0 35.0 41.0 28.0 41.0 58-59 35.57175827132974 39.0 35.0 41.0 28.0 41.0 60-61 35.36187250401707 39.0 35.0 41.0 28.0 41.0 62-63 35.12189761514604 38.0 35.0 40.0 28.0 41.0 64-65 34.817477367965765 37.5 34.5 40.0 27.0 41.0 66-67 34.54872647265999 37.0 34.0 40.0 26.5 41.0 68-69 34.21818865253845 36.5 34.0 39.5 26.0 41.0 70-71 33.90076477157349 36.0 34.0 39.0 26.0 41.0 72-73 33.57469357870973 36.0 34.0 39.0 26.0 41.0 74-75 33.24523697332424 35.0 34.0 38.5 26.0 40.0 76-77 32.9645742083091 35.0 33.5 37.0 26.0 39.5 78-79 32.650896083085854 35.0 33.0 37.0 25.0 39.0 80-81 32.35539095296068 35.0 33.0 36.5 25.0 39.0 82-83 32.08541466861564 35.0 33.0 36.0 24.5 38.0 84-85 31.82366353752289 35.0 33.0 36.0 24.0 37.0 86-87 31.565437181275776 35.0 33.0 35.0 23.5 37.0 88-89 31.36338698196729 35.0 33.0 35.0 23.0 36.5 90-91 31.222337689161684 35.0 33.0 35.0 23.0 36.0 92-93 31.062513163539556 35.0 33.0 35.0 20.5 36.0 94-95 30.872693535031765 35.0 32.5 35.0 19.5 36.0 96-97 30.694207043183074 35.0 32.0 35.0 18.0 35.5 98-99 30.512416387232562 35.0 32.0 35.0 7.5 35.0 100 30.39644157831441 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38166547513582927 1101 2 0.38856356084382426 1101 3 0.49308464525002194 1101 4 0.3372999742769096 1101 5 0.2844554285389691 1101 6 0.23317114591021237 1101 7 0.290054025727585 1101 8 0.2923000771383357 1101 9 0.31947213412089326 1101 10-11 0.42609003448948357 1101 12-13 0.32177413246704134 1101 14-15 0.4518755254975346 1101 16-17 0.4928205806748167 1101 18-19 0.4678582098121211 1101 20-21 0.41949870695611935 1101 22-23 0.4326417554231696 1101 24-25 0.4841151040845517 1101 26-27 0.514577529743768 1101 28-29 0.4951711530636089 1101 30-31 0.5275086528219433 1101 32-33 0.5296013910308943 1101 34-35 0.566183236405827 1101 36-37 0.6000227195948042 1101 38-39 0.5473080070704839 1101 40-41 0.5350022665798093 1101 42-43 0.49693957779390274 1101 44-45 0.5764667623479554 1101 46-47 0.5681955569185675 1101 48-49 0.5327763395736227 1101 50-51 0.5552477089064425 1101 52-53 0.5471313597636822 1101 54-55 0.4859054164704659 1101 56-57 0.49079732209614235 1101 58-59 0.4704322682041209 1101 60-61 0.47787043028277054 1101 62-63 0.42475731125330896 1101 64-65 0.4746961683772071 1101 66-67 0.5305533180556878 1101 68-69 0.5624113263983901 1101 70-71 0.5316217681072146 1101 72-73 0.4713376787961252 1101 74-75 0.49793678851106904 1101 76-77 0.479088357085125 1101 78-79 0.4283292699322132 1101 80-81 0.5168771881875642 1101 82-83 0.5286333097695675 1101 84-85 0.48499030389447384 1101 86-87 0.5497114957141918 1101 88-89 0.5862227127614332 1101 90-91 0.5822978571434625 1101 92-93 0.5963014331081133 1101 94-95 0.5025636565706328 1101 96-97 0.5390268202899868 1101 98-99 0.544144975922098 1101 100 0.5711394130363594 1102 1 0.35884107366823415 1102 2 0.3865451579614394 1102 3 0.47840755061027807 1102 4 0.4234155149850167 1102 5 0.3404531190661686 1102 6 0.29813593718617426 1102 7 0.3310698332365263 1102 8 0.3680367086985612 1102 9 0.40031349054383014 1102 10-11 0.40430044888106664 1102 12-13 0.35796011448141485 1102 14-15 0.4862625005693104 1102 16-17 0.4578431816708459 1102 18-19 0.4799718744712109 1102 20-21 0.45517240061086284 1102 22-23 0.39954782798201904 1102 24-25 0.39602606028237375 1102 26-27 0.32624193805602175 1102 28-29 0.3834109739999363 1102 30-31 0.40841145071166096 1102 32-33 0.4321034060538693 1102 34-35 0.32959521737918607 1102 36-37 0.3805618656492058 1102 38-39 0.3872126922755825 1102 40-41 0.3689319331655838 1102 42-43 0.3676119174963546 1102 44-45 0.36295047469292996 1102 46-47 0.16280776125760354 1102 48-49 0.1560445829043644 1102 50-51 0.26662424827331677 1102 52-53 0.2574472037624034 1102 54-55 0.23016825075187342 1102 56-57 0.2349289031956232 1102 58-59 0.18626276716238266 1102 60-61 0.24325190484881176 1102 62-63 0.23499877734439423 1102 64-65 0.2882140266964015 1102 66-67 0.3665748840038958 1102 68-69 0.3027777537364784 1102 70-71 0.3176123449174 1102 72-73 0.43660644992304043 1102 74-75 0.3309252163610523 1102 76-77 0.2769592522006761 1102 78-79 0.2480625512723691 1102 80-81 0.13839662618403992 1102 82-83 0.21734864431640233 1102 84-85 0.20247417542659818 1102 86-87 0.131166405259183 1102 88-89 0.1843949235633886 1102 90-91 0.15506172508095517 1102 92-93 0.16389692120625554 1102 94-95 0.19959976686917003 1102 96-97 0.25603475406601106 1102 98-99 0.31111172781110596 1102 100 0.36082814185741086 1103 1 0.27636462814887963 1103 2 0.3348363732957118 1103 3 0.41079857755956795 1103 4 0.3514929518302665 1103 5 0.19193861380404087 1103 6 0.25902980898393224 1103 7 0.248448236548235 1103 8 0.2720266174179926 1103 9 0.27587780306580356 1103 10-11 0.27309107197567783 1103 12-13 0.26026314154768926 1103 14-15 0.3788857630309792 1103 16-17 0.3391965335311369 1103 18-19 0.30883692966107645 1103 20-21 0.2907336740501023 1103 22-23 0.29341075644977366 1103 24-25 0.3254996631785332 1103 26-27 0.37209581539787706 1103 28-29 0.41457403350428734 1103 30-31 0.4407538078318538 1103 32-33 0.43308726140369913 1103 34-35 0.4823602422418034 1103 36-37 0.5724291721762995 1103 38-39 0.5036809284568164 1103 40-41 0.5911442264459694 1103 42-43 0.6327002638856598 1103 44-45 0.6632262809876508 1103 46-47 0.7009729500272428 1103 48-49 0.6302010626038665 1103 50-51 0.7025156245899637 1103 52-53 0.6425880601606551 1103 54-55 0.5784740574979992 1103 56-57 0.5866528832750078 1103 58-59 0.5607062112950771 1103 60-61 0.593501675487083 1103 62-63 0.6218676193076362 1103 64-65 0.5692456045461967 1103 66-67 0.6307385354300123 1103 68-69 0.5644355288608907 1103 70-71 0.5795566712106179 1103 72-73 0.535560891021106 1103 74-75 0.4911092906774357 1103 76-77 0.5403580148235179 1103 78-79 0.5483442704267887 1103 80-81 0.4763243786679112 1103 82-83 0.4983880423999949 1103 84-85 0.5450651078972903 1103 86-87 0.588706795495213 1103 88-89 0.622936151136642 1103 90-91 0.5963453433441899 1103 92-93 0.5582813575422385 1103 94-95 0.6278531907646254 1103 96-97 0.675812316098586 1103 98-99 0.7175569674186164 1103 100 0.6988542512656615 1104 1 0.307745745129683 1104 2 0.33766306518031897 1104 3 0.4357942296389581 1104 4 0.3585264380654749 1104 5 0.25653331513812816 1104 6 0.23789311760658194 1104 7 0.2212679355272016 1104 8 0.23373556761256253 1104 9 0.2637168502914662 1104 10-11 0.2713038954428484 1104 12-13 0.25770239747354395 1104 14-15 0.3044937472625122 1104 16-17 0.3377355416255199 1104 18-19 0.37156139106242136 1104 20-21 0.37125816008965273 1104 22-23 0.34345568496270573 1104 24-25 0.383748121478348 1104 26-27 0.384561756686459 1104 28-29 0.40575617876831416 1104 30-31 0.40223675673653503 1104 32-33 0.41308918173530174 1104 34-35 0.32216400609174656 1104 36-37 0.35607093612769347 1104 38-39 0.44074523057933135 1104 40-41 0.45264157783766734 1104 42-43 0.44245092875518566 1104 44-45 0.4725324615567885 1104 46-47 0.5067561373429044 1104 48-49 0.45844424904994696 1104 50-51 0.4974392461179704 1104 52-53 0.479586599983989 1104 54-55 0.38740450526135817 1104 56-57 0.3710325255860738 1104 58-59 0.4297907747276142 1104 60-61 0.4383875244548108 1104 62-63 0.40290733393911893 1104 64-65 0.39893519924752496 1104 66-67 0.3929884394134291 1104 68-69 0.36666247944677366 1104 70-71 0.3896317090737611 1104 72-73 0.4212367571631219 1104 74-75 0.47413355932095413 1104 76-77 0.4279952460676739 1104 78-79 0.4242333296092369 1104 80-81 0.4349524647857308 1104 82-83 0.40974462986204685 1104 84-85 0.38541036902615744 1104 86-87 0.3687193138832896 1104 88-89 0.34661311512861204 1104 90-91 0.34601635068493053 1104 92-93 0.3548434204856399 1104 94-95 0.32045040302286054 1104 96-97 0.366471442746267 1104 98-99 0.41286637631881007 1104 100 0.3904408437278164 1105 1 0.29705927324441106 1105 2 0.32474554556965174 1105 3 0.35644999554037327 1105 4 0.27232364134905396 1105 5 0.162194654546866 1105 6 0.2107817170114501 1105 7 0.22767370941762266 1105 8 0.21967538501205297 1105 9 0.3121248291478551 1105 10-11 0.23613487624856333 1105 12-13 0.23491986388681596 1105 14-15 0.37135656755121715 1105 16-17 0.3510547438150695 1105 18-19 0.33310947736624996 1105 20-21 0.30441895436614175 1105 22-23 0.3841754193141256 1105 24-25 0.369670903494999 1105 26-27 0.42869408942014076 1105 28-29 0.40357764701623466 1105 30-31 0.3977966310553569 1105 32-33 0.3778409773821494 1105 34-35 0.2667933725540763 1105 36-37 0.2822436988767407 1105 38-39 0.2985600538530875 1105 40-41 0.27665573435828605 1105 42-43 0.23719794509599268 1105 44-45 0.23965689833178772 1105 46-47 0.22950622146245792 1105 48-49 0.25381898987716056 1105 50-51 0.22361508698446642 1105 52-53 0.15969512359666993 1105 54-55 0.2374842073559833 1105 56-57 0.21755629711175573 1105 58-59 0.1766192563839155 1105 60-61 0.09496912699324866 1105 62-63 0.01321120287428812 1105 64-65 0.1277833629977394 1105 66-67 0.10714035461340643 1105 68-69 0.1247679146032965 1105 70-71 0.09647391772406166 1105 72-73 0.09560188956447035 1105 74-75 0.1451645735864986 1105 76-77 0.14553544017234543 1105 78-79 0.15015622441187304 1105 80-81 0.1071092241436773 1105 82-83 0.1790371421886121 1105 84-85 0.19337578508424613 1105 86-87 0.21023162823286157 1105 88-89 0.13155961697736274 1105 90-91 0.15261282687486855 1105 92-93 0.16602229717484462 1105 94-95 0.20082477259621 1105 96-97 0.18352705657457946 1105 98-99 0.22037700639770463 1105 100 0.19659252019746276 1106 1 0.21501539156615124 1106 2 0.23285503509331917 1106 3 0.36129533517075174 1106 4 0.28361339967838006 1106 5 0.21422330473717466 1106 6 0.18520230144170569 1106 7 0.19244007474843983 1106 8 0.2254833410508681 1106 9 0.24006484139211182 1106 10-11 0.26893354573662265 1106 12-13 0.2408013649550682 1106 14-15 0.37333101022754533 1106 16-17 0.2987938730646391 1106 18-19 0.3322863409518604 1106 20-21 0.3447263441934183 1106 22-23 0.3910848186528426 1106 24-25 0.3747813459805158 1106 26-27 0.4477676094613017 1106 28-29 0.4946406765262523 1106 30-31 0.46634218865162325 1106 32-33 0.5523677619707428 1106 34-35 0.5903832389008912 1106 36-37 0.6509676670598594 1106 38-39 0.6581985984906638 1106 40-41 0.7278263497399351 1106 42-43 0.7665064911987898 1106 44-45 0.7703188149550115 1106 46-47 0.761657069984679 1106 48-49 0.7641714892155349 1106 50-51 0.8271293183736006 1106 52-53 0.7977213680978323 1106 54-55 0.7287842847060517 1106 56-57 0.764223259094365 1106 58-59 0.7486369060551539 1106 60-61 0.7374081300520388 1106 62-63 0.6590734288977558 1106 64-65 0.6460492791075652 1106 66-67 0.6669771016855179 1106 68-69 0.6350429786925318 1106 70-71 0.7202527010905087 1106 72-73 0.7081523062439103 1106 74-75 0.6931803911630254 1106 76-77 0.7057487434743237 1106 78-79 0.7200389385471695 1106 80-81 0.7054550856882003 1106 82-83 0.6512483941805769 1106 84-85 0.6783358265647799 1106 86-87 0.7233510960280896 1106 88-89 0.724568948961327 1106 90-91 0.7127122915579207 1106 92-93 0.7113755291239414 1106 94-95 0.7044786543539772 1106 96-97 0.7353663070172693 1106 98-99 0.7994127259974846 1106 100 0.8530509664039592 1107 1 0.17404504746941996 1107 2 0.15369389833383806 1107 3 0.2851810797664882 1107 4 0.18200529709679358 1107 5 0.1916666322425158 1107 6 0.19056360914078851 1107 7 0.1417484045437476 1107 8 0.16611342174015675 1107 9 0.16022250921434988 1107 10-11 0.15838108990066502 1107 12-13 0.1335396635600219 1107 14-15 0.16243767809770304 1107 16-17 0.2022790517990387 1107 18-19 0.16746844622792167 1107 20-21 0.2466082120038351 1107 22-23 0.20135258873797568 1107 24-25 0.23229759025699082 1107 26-27 0.26467675628995124 1107 28-29 0.21503344201472885 1107 30-31 0.2465335039393537 1107 32-33 0.26681335604156686 1107 34-35 0.25963714995213394 1107 36-37 0.24885719260774408 1107 38-39 0.2856362707256821 1107 40-41 0.2738407770867042 1107 42-43 0.21926200076929803 1107 44-45 0.20292286914308733 1107 46-47 0.1646426905544871 1107 48-49 0.15950959637705608 1107 50-51 0.276498741803465 1107 52-53 0.27311233392885725 1107 54-55 0.14138336337057922 1107 56-57 0.22724648303304207 1107 58-59 0.18004193150107994 1107 60-61 0.14176146242704135 1107 62-63 0.18029517901169356 1107 64-65 0.24422262679357942 1107 66-67 0.20159914019039604 1107 68-69 0.2528704801328132 1107 70-71 0.32651194313942256 1107 72-73 0.3091649339332321 1107 74-75 0.2276928622877108 1107 76-77 0.2291274916927719 1107 78-79 0.18842386020356372 1107 80-81 0.19360244698921747 1107 82-83 0.19344389826852648 1107 84-85 0.1811693120053839 1107 86-87 0.2715615233436566 1107 88-89 0.25924536720840763 1107 90-91 0.20336621910137254 1107 92-93 0.21061811291944466 1107 94-95 0.19009819339651557 1107 96-97 0.2786918622645267 1107 98-99 0.36308304658166435 1107 100 0.38782692298039834 1108 1 0.07174351992242833 1108 2 0.14774603556063326 1108 3 0.19029382937301875 1108 4 0.11167316153824913 1108 5 0.15604157599392465 1108 6 0.11589160699840306 1108 7 0.11314547676364128 1108 8 0.12897783026842546 1108 9 0.11465566613748024 1108 10-11 0.09707638609200586 1108 12-13 0.0990470422770926 1108 14-15 0.09520497687043417 1108 16-17 0.1624038523223419 1108 18-19 0.16643747822541144 1108 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-0.9269836562478346 2215 36-37 -0.9450675310463339 2215 38-39 -0.831093070342142 2215 40-41 -0.8493327690366073 2215 42-43 -0.9671199671401212 2215 44-45 -1.0533749749643633 2215 46-47 -1.005374900175596 2215 48-49 -1.0574693491576852 2215 50-51 -1.088845548073877 2215 52-53 -1.2529680165444574 2215 54-55 -1.082183248365041 2215 56-57 -1.134862628842825 2215 58-59 -1.1137557386182095 2215 60-61 -1.1985628557186416 2215 62-63 -1.1586911174700276 2215 64-65 -1.1385949247944325 2215 66-67 -0.9779057612142097 2215 68-69 -1.0010352376874891 2215 70-71 -1.1650590159803187 2215 72-73 -0.976075945818387 2215 74-75 -1.0841649895975323 2215 76-77 -0.9721016766522652 2215 78-79 -1.0188726970241966 2215 80-81 -1.1341737924286122 2215 82-83 -1.0672814699642288 2215 84-85 -1.0294698213690872 2215 86-87 -1.0445991084294874 2215 88-89 -0.9964187909356497 2215 90-91 -0.9741152000177529 2215 92-93 -1.1170938563270418 2215 94-95 -1.1881881793025713 2215 96-97 -1.2973562529481164 2215 98-99 -1.26527539939406 2215 100 -1.3598714772986575 2216 1 -0.8752201557052288 2216 2 -1.3427525444479649 2216 3 -0.9912730992399261 2216 4 -1.394458082525432 2216 5 -0.7803131572319799 2216 6 -0.9271190110404461 2216 7 -0.5990692845313177 2216 8 -0.6310081824618763 2216 9 -0.7350573821894812 2216 10-11 -0.9497947892639331 2216 12-13 -0.7598618089238727 2216 14-15 -0.8983799579895191 2216 16-17 -0.9848184814725087 2216 18-19 -1.0347537433452274 2216 20-21 -0.8991094312126577 2216 22-23 -0.9280875513811466 2216 24-25 -0.9916161349422907 2216 26-27 -1.0692935551051264 2216 28-29 -1.348789086295227 2216 30-31 -1.3601943620765624 2216 32-33 -1.220098647534627 2216 34-35 -1.0776044379874534 2216 36-37 -1.0347280657898636 2216 38-39 -1.2670866298479382 2216 40-41 -1.0533520283207523 2216 42-43 -1.7231403378249297 2216 44-45 -1.5677243243318983 2216 46-47 -1.3044377006691832 2216 48-49 -1.2263464077232413 2216 50-51 -1.3927617599046656 2216 52-53 -1.4596599946651168 2216 54-55 -1.5432274746658265 2216 56-57 -1.6567226722637187 2216 58-59 -1.4939307547126361 2216 60-61 -1.696159814984533 2216 62-63 -1.669053407754447 2216 64-65 -1.690817533171888 2216 66-67 -1.5928080414923897 2216 68-69 -1.836108747670579 2216 70-71 -1.5847638932495443 2216 72-73 -1.4276854110657737 2216 74-75 -1.539931254501333 2216 76-77 -1.7150615005170522 2216 78-79 -1.6835855783758191 2216 80-81 -1.4603950278993665 2216 82-83 -1.6013555138554771 2216 84-85 -1.610837640962611 2216 86-87 -1.6906931911289576 2216 88-89 -1.5232525566316895 2216 90-91 -1.3868117381499658 2216 92-93 -1.3488429140426952 2216 94-95 -1.5781421478837103 2216 96-97 -1.7809538995753655 2216 98-99 -1.6704371812853154 2216 100 -1.6489350768384803 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 133.0 3 19.0 4 35.0 5 98.0 6 281.0 7 803.0 8 1631.0 9 2657.0 10 3839.0 11 5425.0 12 8360.0 13 12122.0 14 12042.0 15 13368.0 16 16715.0 17 23943.0 18 24091.0 19 12937.0 20 9256.0 21 8889.0 22 9103.0 23 9793.0 24 11149.0 25 13581.0 26 16755.0 27 21222.0 28 26804.0 29 34528.0 30 44388.0 31 56616.0 32 73171.0 33 94935.0 34 129207.0 35 188670.0 36 297233.0 37 551299.0 38 711652.0 39 254051.0 40 790.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.448843701237536 19.545539900538937 13.557171963491188 22.448444434732334 2 17.247239214298563 21.067883828240152 38.36946149410013 23.315415463361152 3 18.276785790299126 25.49734582325748 30.76598197136428 25.459886415079115 4 12.99768151347954 18.13389259655666 37.40716418264456 31.461261707319245 5 11.8356304131042 35.98532099768059 34.32771457528393 17.851334013931282 6 28.654322875004578 37.69891843451178 18.349449408061382 15.297309282422255 7 25.29246655026612 32.60867392584591 23.035500192294094 19.063359331593865 8 24.697076648537845 33.436408397866295 22.103049647411467 19.763465306184393 9 24.08359521615049 16.661706624617356 23.377973711777138 35.87672444745502 10-11 23.08724747750492 26.232394170694235 28.968818744214058 21.71153960758679 12-13 24.826491066372025 24.047042667466194 29.189736414481843 21.936729851679935 14-15 22.26046513349546 26.106718636062464 27.8905940575735 23.742222172868573 16-17 20.200744672306058 29.034946444521026 28.15402109349639 22.610287789676526 18-19 19.7280787506325 26.369498565845088 32.28536443895468 21.617058244567737 20-21 21.479472651485736 26.286714014075407 31.728470371717997 20.505342962720857 22-23 20.890134739122242 26.66630515129788 31.82102694301247 20.622533166567404 24-25 20.581039098812514 26.194324011295567 32.195324902992354 21.02931198689957 26-27 20.413175051293848 25.83101957328108 32.40266568847765 21.35313968694743 28-29 20.625117754860383 26.050799030347715 32.228094079516964 21.095989135274934 30-31 21.221883161878367 26.997772417959244 31.36511764949748 20.415226770664912 32-33 20.220603340772158 26.359504454967464 32.34605090111716 21.073841303143222 34-35 20.233891806716855 25.610057184821834 32.381381933830845 21.774669074630467 36-37 21.589926084296255 26.63785895052212 31.079593469181678 20.692621495999948 38-39 21.25436455777355 26.56843689514808 31.398886804109132 20.778311742969237 40-41 20.569268257112196 26.364094342926077 31.664230447910143 21.402406952051585 42-43 20.514781845216394 26.55459320082129 31.311345795866213 21.6192791580961 44-45 21.256715024591067 26.800448328410926 30.84800771101177 21.09482893598624 46-47 21.04198970162397 27.75166559260821 29.943244554782716 21.263100150985103 48-49 21.019428921698363 26.975770943862337 30.39753241700909 21.60726771743021 50-51 21.169618199053815 26.663103334294497 30.129246062783004 22.038032403868684 52-53 21.118796294479807 27.044619263241547 29.810211834433858 22.026372607844785 54-55 21.50260716740617 27.900577844684854 29.404617501316814 21.19219748659216 56-57 20.713572113617495 27.474310508141315 29.482960226029775 22.32915715221142 58-59 20.503086430606345 26.709853683959793 30.238228801737943 22.548831083695912 60-61 21.51071350178469 27.41216194457266 29.312838249757274 21.764286303885378 62-63 21.164917265418783 27.27796324462141 29.557120230264317 21.999999259695493 64-65 21.04989245226239 26.81917803250011 30.065579874969973 22.06534964026753 66-67 21.022908352892795 27.036568451701235 29.46748786178219 22.473035333623777 68-69 21.839186612629373 27.35425162432063 29.124467767326735 21.68209399572326 70-71 21.95004721292009 26.748867611714726 29.42379138811167 21.87729378725351 72-73 21.304890340543775 27.077988489005183 29.933639103772553 21.683482066678486 74-75 21.27959043393319 27.013174829202498 29.63572206155558 22.071512675308735 76-77 21.425671021261174 27.312683526114796 29.058358574632503 22.203286877991523 78-79 21.649564672300766 27.598478745166492 29.101577573613717 21.65037900891902 80-81 21.246243175530203 27.07893352176603 29.43777232871018 22.237050973993586 82-83 20.99988081057691 27.23002670287261 29.52501534286207 22.245077143688402 84-85 21.278405946718063 27.181612612715988 29.247247270219663 22.292734170346286 86-87 21.53856745895289 27.334744600496524 29.109421078172083 22.017266862378502 88-89 21.014969526262046 27.22237305940561 29.282827492047737 22.479829922284612 90-91 20.71072259894503 27.00257644715322 29.26561539627519 23.02108555762656 92-93 21.11581022964235 27.42079098065366 28.853402122950335 22.609996666753656 94-95 21.050425261583765 27.047210467237136 29.2058791685213 22.696485102657803 96-97 20.76770688087131 27.056945333323956 29.373025006375876 22.802322779428863 98-99 20.830315047921797 27.010899881514177 29.26733118916312 22.89145388140091 100 21.167625992485796 27.23377320427162 28.855879032407323 22.74272177083526 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1323.0 1 1039.5 2 3371.5 3 5447.0 4 3074.0 5 1171.0 6 1738.0 7 3890.5 8 6026.5 9 6620.5 10 5231.0 11 3332.0 12 2813.0 13 2967.0 14 3302.0 15 3725.0 16 4135.5 17 4581.0 18 4994.5 19 5422.0 20 6103.5 21 6973.5 22 8288.5 23 10570.0 24 13166.0 25 16008.0 26 20262.0 27 25904.0 28 33161.0 29 40469.0 30 46010.5 31 51732.0 32 57827.5 33 63469.5 34 68938.5 35 74009.0 36 77978.0 37 80872.5 38 83588.0 39 85229.5 40 86531.0 41 86439.0 42 84648.0 43 84112.0 44 83551.5 45 82939.0 46 83348.0 47 84018.0 48 85208.0 49 86844.5 50 86947.5 51 84090.0 52 79113.0 53 75180.0 54 72849.0 55 70037.0 56 65951.0 57 60909.5 58 56705.5 59 52292.5 60 45914.0 61 38214.5 62 30681.0 63 23960.5 64 18266.5 65 13774.5 66 10160.5 67 7266.5 68 5118.5 69 3740.0 70 2675.0 71 1879.5 72 1333.0 73 933.0 74 681.5 75 464.0 76 270.5 77 171.0 78 116.5 79 68.0 80 39.5 81 19.5 82 7.5 83 5.5 84 4.0 85 2.0 86 2.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06070496977521764 2 1.850761273634684E-4 3 0.0 4 0.12137292432496259 5 0.0016656851462712157 6 0.0016286699207985222 7 0.0 8 0.0 9 3.701522547269368E-5 10-11 0.0 12-13 3.701522547269368E-5 14-15 3.701522547269368E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.4806090189077472E-4 30-31 2.5910657830885577E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.776141910452026E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.0358374009981527E-4 80-81 2.776141910452026E-4 82-83 3.331370292542432E-4 84-85 0.0 86-87 0.0 88-89 9.25380636817342E-5 90-91 9.25380636817342E-5 92-93 7.588121221902205E-4 94-95 6.847816712448331E-4 96-97 0.0 98-99 7.403045094538736E-5 100 0.001517624244380441 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2701591.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.90026050724803 #Duplication Level Percentage of deduplicated Percentage of total 1 84.11359947026826 59.6367611464434 2 10.597877544823312 15.027845575037743 3 2.494158037397291 5.305093637931433 4 0.9597362992027434 2.721822145269466 5 0.511613664047019 1.8136771030000665 6 0.308410661720468 1.311983775551636 7 0.20448860969030092 1.014880698846511 8 0.1464016329347695 0.8303931131009329 9 0.10813466399151532 0.6900098262175953 >10 0.5152135199793594 6.324609416325365 >50 0.026213434905528715 1.2707953730046553 >100 0.012899237958726929 1.647632133355087 >500 7.310467970845743E-4 0.34930666988115217 >1k 2.6108814179549313E-4 0.36570760835952404 >5k 1.566528850772959E-4 0.7612923213906553 >10k+ 1.0443525671819726E-4 0.9281894562848378 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12789 0.4733877185702795 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12282 0.4546209992556238 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7898 0.2923462507833347 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7581 0.2806124243084908 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5084 0.18818540630317468 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3613 0.13373600963284227 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 3.701522547269368E-5 0.0 0.0 7.403045094538736E-5 0.0 9 3.701522547269368E-5 0.0 0.0 7.403045094538736E-5 0.0 10-11 3.701522547269368E-5 0.0 0.0 9.25380636817342E-5 0.0 12-13 3.701522547269368E-5 0.0 0.0 1.1104567641808104E-4 0.0 14-15 3.701522547269368E-5 0.0 0.0 1.4806090189077472E-4 0.0 16-17 3.701522547269368E-5 0.0 0.0 1.850761273634684E-4 0.0 18-19 3.701522547269368E-5 0.0 0.0 1.850761273634684E-4 0.0 20-21 3.701522547269368E-5 0.0 0.0 2.5910657830885577E-4 0.0 22-23 7.403045094538736E-5 0.0 0.0 5.922436075630989E-4 0.0 24-25 1.1104567641808104E-4 1.850761273634684E-5 0.0 0.0010734415387081168 0.0 26-27 1.1104567641808104E-4 3.701522547269368E-5 0.0 0.0020358374009981526 0.0 28-29 1.1104567641808104E-4 3.701522547269368E-5 0.0 0.004386304218514202 0.0 30-31 1.1104567641808104E-4 3.701522547269368E-5 0.0 0.011493227509271389 0.0 32-33 1.1104567641808104E-4 3.701522547269368E-5 0.0 0.027002606982330042 0.0 34-35 1.1104567641808104E-4 3.701522547269368E-5 0.0 0.050118615290027244 0.0 36-37 1.1104567641808104E-4 3.701522547269368E-5 0.0 0.08348784105366061 0.0 38-39 1.4806090189077472E-4 5.552283820904052E-5 0.0 0.12988642618368212 0.0 40-41 1.4806090189077472E-4 7.403045094538736E-5 0.0 0.19397828908965126 0.0 42-43 1.4806090189077472E-4 7.403045094538736E-5 0.0 0.2613274918372174 0.0 44-45 1.4806090189077472E-4 7.403045094538736E-5 0.0 0.33589466355195885 0.0 46-47 1.4806090189077472E-4 7.403045094538736E-5 0.0 0.4222881998052259 0.0 48-49 1.4806090189077472E-4 7.403045094538736E-5 0.0 0.5187868926125383 0.0 50-51 1.4806090189077472E-4 7.403045094538736E-5 0.0 0.6187465090015476 0.0 52-53 1.4806090189077472E-4 7.403045094538736E-5 0.0 0.7148565419413968 0.0 54-55 1.4806090189077472E-4 7.403045094538736E-5 0.0 0.8225708480669354 0.0 56-57 1.4806090189077472E-4 7.403045094538736E-5 0.0 0.9482005233212577 0.0 58-59 1.4806090189077472E-4 7.403045094538736E-5 0.0 1.0876738929023677 0.0 60-61 1.4806090189077472E-4 7.403045094538736E-5 0.0 1.228627871502385 0.0 62-63 1.4806090189077472E-4 7.403045094538736E-5 0.0 1.370988428670365 0.0 64-65 1.4806090189077472E-4 7.403045094538736E-5 0.0 1.5257490863716971 0.0 66-67 1.4806090189077472E-4 7.403045094538736E-5 0.0 1.7083451936284952 0.0 68-69 1.6656851462712157E-4 1.1104567641808104E-4 0.0 1.8995103255822219 0.0 70-71 2.220913528361621E-4 1.4806090189077472E-4 0.0 2.1046672127646264 0.0 72-73 2.220913528361621E-4 1.850761273634684E-4 0.0 2.3378076104043877 0.0 74-75 2.220913528361621E-4 1.850761273634684E-4 0.0 2.5691527696087233 0.0 76-77 2.220913528361621E-4 1.850761273634684E-4 0.0 2.805569014702818 0.0 78-79 2.220913528361621E-4 1.850761273634684E-4 0.0 3.0694505570976514 0.0 80-81 2.220913528361621E-4 1.850761273634684E-4 0.0 3.355282128197792 0.0 82-83 2.220913528361621E-4 1.850761273634684E-4 0.0 3.6576780126969624 0.0 84-85 2.220913528361621E-4 1.850761273634684E-4 0.0 3.958667318628171 0.0 86-87 2.220913528361621E-4 1.850761273634684E-4 0.0 4.275536156287165 0.0 88 2.220913528361621E-4 1.850761273634684E-4 0.0 4.534846318336121 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6800 0.0 59.421524 1 ATAACGC 415 0.0 39.69058 3 TATAACG 405 0.0 38.34656 2 GTATCAA 11885 0.0 37.920856 1 GTGGTAT 2585 0.0 36.071987 1 TGGTATC 2645 0.0 34.517834 2 TACCTGG 2295 0.0 30.964336 2 ATCAACG 15070 0.0 29.917118 3 TATCAAC 15085 0.0 29.793777 2 TCAACGC 15225 0.0 29.581635 4 GTACCTG 2490 0.0 29.504663 1 CAACGCA 15680 0.0 28.893171 5 TAACGCA 600 0.0 28.237013 4 AACGCAG 16545 0.0 27.638235 6 CGCAGAG 18430 0.0 24.505428 8 ACGCAGA 18545 0.0 24.480175 7 TCTGTCG 990 0.0 23.260826 8 GCAGAGT 19290 0.0 23.16928 9 GTATAAC 850 0.0 22.161875 1 GTACATG 7135 0.0 22.045397 1 >>END_MODULE