FastQCFastQC Report
Wed 25 May 2016
SRR2049516_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049516_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7782041
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73950.040.9114721
GTATCAA114500.034.8279381
TCAACGC115050.033.454734
ATCAACG116500.033.038343
CAACGCA126300.030.51515
AACGCAG136800.028.172326
TATCAAC137800.028.117262
ACGCAGA158950.023.8920787
GTACATG200950.023.1564351
CGCAGAG164150.023.0656728
TACATGG204800.021.7178172
ACATGGG209050.020.7376523
GTGGTAT46400.020.1896571
GCAGAGT194200.018.471649
GAGTACA156900.018.2383141
CATGGGG145100.017.46084
AGAGTAC163400.016.70742610-11
CAGAGTA183850.016.22689610-11
AGTACAT164400.015.971362
TGGTATC52000.015.551782
Produced by FastQC (version 0.11.5)