FastQCFastQC Report
Wed 25 May 2016
SRR2049516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049516_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7782041
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243890.3134010730603964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235840.3030567430832091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137810.17708721914983486No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140800.058.1026571
GTATCAA256800.041.99321
ATCAACG321100.033.1109123
TCAACGC322850.032.960584
TATCAAC330400.032.2645572
CAACGCA334750.031.8145945
GTGGTAT55250.030.6703781
AACGCAG351100.030.3590436
TGGTATC61200.026.6737382
ACGCAGA397800.026.6414387
CGCAGAG399300.026.4236588
GCAGAGT428000.024.2345229
GTACATG207100.022.9784091
TACATGG209450.021.9891382
GAGTACT316650.021.74372912-13
CAGAGTA418800.021.49007810-11
AGAGTAC399950.021.08694510-11
ACATGGG211350.020.7228973
GTACTTT348200.019.61158814-15
AGTACTT332800.019.47407512-13
Produced by FastQC (version 0.11.5)