##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049516_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7782041 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85787147613332 31.0 31.0 34.0 30.0 34.0 2 32.02872781060907 33.0 31.0 34.0 30.0 34.0 3 31.986251421702868 33.0 31.0 34.0 30.0 34.0 4 35.65038734696977 37.0 35.0 37.0 33.0 37.0 5 35.76249238470987 37.0 35.0 37.0 35.0 37.0 6 35.81240050007447 37.0 35.0 37.0 35.0 37.0 7 35.825868175199794 37.0 35.0 37.0 35.0 37.0 8 35.81573458685196 37.0 35.0 37.0 35.0 37.0 9 37.565597508417135 39.0 37.0 39.0 35.0 39.0 10-11 37.519855074009506 39.0 37.0 39.0 35.0 39.0 12-13 37.499620922069155 39.0 37.0 39.0 35.0 39.0 14-15 38.784908997523914 40.0 38.0 41.0 35.0 41.0 16-17 38.790606988577935 40.0 38.0 41.0 35.0 41.0 18-19 38.86297996887963 40.0 38.0 41.0 35.0 41.0 20-21 38.891608448220715 40.0 38.0 41.0 35.0 41.0 22-23 38.86663010385065 40.0 38.0 41.0 35.0 41.0 24-25 38.828672002627584 40.0 38.0 41.0 35.0 41.0 26-27 38.74019874734661 40.0 38.0 41.0 34.5 41.0 28-29 38.65838486330257 40.0 38.0 41.0 34.0 41.0 30-31 38.545598898797884 40.0 38.0 41.0 34.0 41.0 32-33 38.40352408834649 40.0 38.0 41.0 34.0 41.0 34-35 38.305928354785074 40.0 38.0 41.0 34.0 41.0 36-37 38.171892309485386 40.0 38.0 41.0 34.0 41.0 38-39 38.084005339987286 40.0 38.0 41.0 33.5 41.0 40-41 37.96378366549341 40.0 38.0 41.0 33.0 41.0 42-43 37.825988002376235 40.0 37.0 41.0 33.0 41.0 44-45 37.64701034086045 40.0 37.0 41.0 33.0 41.0 46-47 37.40567532347876 40.0 37.0 41.0 32.5 41.0 48-49 37.18072521077697 40.0 37.0 41.0 32.0 41.0 50-51 36.97477339171048 40.0 37.0 41.0 31.0 41.0 52-53 36.87550232644624 40.0 36.0 41.0 31.0 41.0 54-55 36.74835104312609 40.0 36.0 41.0 31.0 41.0 56-57 36.58196243119254 39.0 35.5 41.0 31.0 41.0 58-59 36.412555909176014 39.0 35.0 41.0 31.0 41.0 60-61 36.22944501320411 39.0 35.0 41.0 30.0 41.0 62-63 36.035215890021654 39.0 35.0 41.0 30.0 41.0 64-65 35.76548722886451 38.0 35.0 40.5 30.0 41.0 66-67 35.51664415543429 38.0 35.0 40.0 29.0 41.0 68-69 35.21209943509678 37.0 35.0 40.0 29.0 41.0 70-71 34.92632472637962 37.0 34.5 39.5 29.0 41.0 72-73 34.60856014508276 36.0 34.0 39.0 29.0 41.0 74-75 34.27075583384873 36.0 34.0 39.0 29.0 41.0 76-77 33.97870777087913 35.0 34.0 38.5 29.0 40.0 78-79 33.649645896237246 35.0 34.0 37.0 28.0 39.0 80-81 33.34025488943068 35.0 34.0 37.0 28.0 39.0 82-83 33.04388520954849 35.0 34.0 36.5 28.0 39.0 84-85 32.76736360037168 35.0 34.0 36.0 27.0 37.0 86-87 32.4896817557245 35.0 33.0 36.0 27.0 37.0 88-89 32.27957427877854 35.0 33.0 35.0 26.5 37.0 90-91 32.130391243119895 35.0 33.0 35.0 26.0 36.0 92-93 31.975542662908097 35.0 33.0 35.0 26.0 36.0 94-95 31.795421728053093 35.0 33.0 35.0 26.0 36.0 96-97 31.630706648808456 35.0 33.0 35.0 25.5 36.0 98-99 31.455373532470468 35.0 33.0 35.0 25.0 35.0 100 31.344786027213168 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.374640803098675 1101 2 0.3807243243970646 1101 3 0.46795617203472517 1101 4 0.36146651066446367 1101 5 0.27374999924925447 1101 6 0.2545939097400165 1101 7 0.24495980625523117 1101 8 0.28002144723377853 1101 9 0.31863869282917534 1101 10-11 0.34720014946874755 1101 12-13 0.2734729833662328 1101 14-15 0.43296700344235717 1101 16-17 0.44316579633402853 1101 18-19 0.4442511660118029 1101 20-21 0.41138308842504756 1101 22-23 0.411837885922651 1101 24-25 0.46612511038731697 1101 26-27 0.41767748866645604 1101 28-29 0.4680374783817598 1101 30-31 0.44686219486749224 1101 32-33 0.48802238679297716 1101 34-35 0.4988590069726442 1101 36-37 0.5019190796468322 1101 38-39 0.50368525592922 1101 40-41 0.49238906758393597 1101 42-43 0.3999546582109659 1101 44-45 0.43902787484528005 1101 46-47 0.517770124548484 1101 48-49 0.4980263413077495 1101 50-51 0.48253710907115277 1101 52-53 0.4351353669635287 1101 54-55 0.37877805744280124 1101 56-57 0.39640070265622285 1101 58-59 0.3969431159058914 1101 60-61 0.45530905729788884 1101 62-63 0.43352312899625645 1101 64-65 0.44062603567169134 1101 66-67 0.4280217038224663 1101 68-69 0.39945008781622704 1101 70-71 0.4005104515212565 1101 72-73 0.41069858244939184 1101 74-75 0.4175013316146803 1101 76-77 0.4111514553756024 1101 78-79 0.3884976453724178 1101 80-81 0.46044953315947623 1101 82-83 0.45087607080934333 1101 84-85 0.40393388436231703 1101 86-87 0.4745147636305376 1101 88-89 0.430858096365931 1101 90-91 0.43887164049755967 1101 92-93 0.47650430506537234 1101 94-95 0.41999893315196957 1101 96-97 0.3935146447422575 1101 98-99 0.4385226534502742 1101 100 0.4710998375578477 1102 1 0.2839114945966408 1102 2 0.30889514469277657 1102 3 0.36282615683855823 1102 4 0.3298263085443409 1102 5 0.26620514347619206 1102 6 0.24070176368589813 1102 7 0.2607673299547173 1102 8 0.2720957108539892 1102 9 0.3573096901231878 1102 10-11 0.274118969860794 1102 12-13 0.2977160061471622 1102 14-15 0.41545489731102947 1102 16-17 0.4209383567425746 1102 18-19 0.40839324176639735 1102 20-21 0.3929938653419356 1102 22-23 0.35955064735048836 1102 24-25 0.3957384046321337 1102 26-27 0.3357480979349674 1102 28-29 0.31195248427376043 1102 30-31 0.4026313082694344 1102 32-33 0.4236614092899629 1102 34-35 0.42590206570575617 1102 36-37 0.4384308958915071 1102 38-39 0.3980300325939794 1102 40-41 0.44452399862166203 1102 42-43 0.4674538221454725 1102 44-45 0.4930044486465661 1102 46-47 0.5281927411565803 1102 48-49 0.5166482877390735 1102 50-51 0.48237747950520315 1102 52-53 0.462979137924485 1102 54-55 0.4438953341599614 1102 56-57 0.49917128177197867 1102 58-59 0.4608224716025475 1102 60-61 0.4499885511852355 1102 62-63 0.418408600149796 1102 64-65 0.41052390725077004 1102 66-67 0.4871862237546907 1102 68-69 0.42180390415310853 1102 70-71 0.43230240443148205 1102 72-73 0.46596709834235384 1102 74-75 0.42272090672849316 1102 76-77 0.4323040662935185 1102 78-79 0.386418614417984 1102 80-81 0.38427798808387337 1102 82-83 0.36517469927389357 1102 84-85 0.37764396574104353 1102 86-87 0.37139200998320376 1102 88-89 0.38104278997118257 1102 90-91 0.3744816785472409 1102 92-93 0.42357767093534804 1102 94-95 0.3836152018455614 1102 96-97 0.3901809609130602 1102 98-99 0.43595866022349483 1102 100 0.5001110423327582 1103 1 0.29227469259405936 1103 2 0.2928214334235548 1103 3 0.3828433182768123 1103 4 0.29823773491548167 1103 5 0.22984983173197548 1103 6 0.2432615804795617 1103 7 0.23785962091774593 1103 8 0.251209192937317 1103 9 0.2955256407153968 1103 10-11 0.29165213141795476 1103 12-13 0.28834892670401757 1103 14-15 0.3706403878670841 1103 16-17 0.3478234303157137 1103 18-19 0.3464942232607271 1103 20-21 0.35361447197026763 1103 22-23 0.35026648317582953 1103 24-25 0.3294986100355004 1103 26-27 0.38097030782101626 1103 28-29 0.3476367081474052 1103 30-31 0.3649238305053899 1103 32-33 0.3299043425328563 1103 34-35 0.3665264419453109 1103 36-37 0.3685579362390925 1103 38-39 0.37452691103634805 1103 40-41 0.3773319929478305 1103 42-43 0.3418340355693772 1103 44-45 0.364479331286077 1103 46-47 0.35062386940891344 1103 48-49 0.335110080080014 1103 50-51 0.4104922269564213 1103 52-53 0.40667388677918836 1103 54-55 0.39183257417761297 1103 56-57 0.41722323000777095 1103 58-59 0.3443572991499124 1103 60-61 0.3291905099270096 1103 62-63 0.34771502201823523 1103 64-65 0.37285863538070174 1103 66-67 0.3772922278919566 1103 68-69 0.38170298615974474 1103 70-71 0.38044804489834405 1103 72-73 0.4233841825682845 1103 74-75 0.38194117558833085 1103 76-77 0.3698714374770731 1103 78-79 0.3253871120762355 1103 80-81 0.2836371339514443 1103 82-83 0.34621042018271453 1103 84-85 0.34405937892097427 1103 86-87 0.38637512256980244 1103 88-89 0.3770860935824274 1103 90-91 0.3397887977128633 1103 92-93 0.35717067062168795 1103 94-95 0.38634010091644555 1103 96-97 0.40403100602098974 1103 98-99 0.39068344523532517 1103 100 0.408657321287631 1104 1 0.33693460501232764 1104 2 0.2688207878640583 1104 3 0.3606202328104047 1104 4 0.26809369445220455 1104 5 0.20845481646153274 1104 6 0.2343496870313686 1104 7 0.20881974591481622 1104 8 0.21930207589023354 1104 9 0.2401147371661736 1104 10-11 0.22604011729696083 1104 12-13 0.2897745013320545 1104 14-15 0.3513210419642263 1104 16-17 0.349697855133293 1104 18-19 0.36705929340988064 1104 20-21 0.3694089082391301 1104 22-23 0.35467941056640484 1104 24-25 0.33739126790498375 1104 26-27 0.3244127931203735 1104 28-29 0.36005269070375334 1104 30-31 0.3785183633925868 1104 32-33 0.388345410280877 1104 34-35 0.31923647130964383 1104 36-37 0.34564786581889706 1104 38-39 0.3796563024425268 1104 40-41 0.3890469767349245 1104 42-43 0.3559821988663643 1104 44-45 0.3720155723988512 1104 46-47 0.3928992502766917 1104 48-49 0.40069790496953317 1104 50-51 0.48422076194907504 1104 52-53 0.4428178961348763 1104 54-55 0.3856859607162164 1104 56-57 0.35186921638942437 1104 58-59 0.35373746578188303 1104 60-61 0.3403900779010911 1104 62-63 0.370478193772783 1104 64-65 0.4048126217106116 1104 66-67 0.41994086134475594 1104 68-69 0.3492071156153713 1104 70-71 0.34885879121563335 1104 72-73 0.3549639307261643 1104 74-75 0.3686304532639255 1104 76-77 0.35207784188313695 1104 78-79 0.31757486039796134 1104 80-81 0.324026890499141 1104 82-83 0.3539877451209037 1104 84-85 0.3877873148615123 1104 86-87 0.36193076265313096 1104 88-89 0.3346443294555712 1104 90-91 0.35421675607550895 1104 92-93 0.3505185164672504 1104 94-95 0.3061697960990237 1104 96-97 0.3164438162191061 1104 98-99 0.39348202728359283 1104 100 0.434770233832797 1105 1 0.2595471318132603 1105 2 0.3072900016835405 1105 3 0.3860802957072167 1105 4 0.2878056846405386 1105 5 0.220449949233803 1105 6 0.23506391335300947 1105 7 0.19445312399061265 1105 8 0.23167009057209498 1105 9 0.26011375718744034 1105 10-11 0.25261670263528657 1105 12-13 0.2540020850608471 1105 14-15 0.32805072670804236 1105 16-17 0.28072381120648515 1105 18-19 0.2907393400034124 1105 20-21 0.28839514621954265 1105 22-23 0.3072008735855718 1105 24-25 0.29617192193685327 1105 26-27 0.34577916930748387 1105 28-29 0.32455987647208673 1105 30-31 0.33943794722827647 1105 32-33 0.3430875199672272 1105 34-35 0.3171449108568396 1105 36-37 0.37480111222118495 1105 38-39 0.34896181304114293 1105 40-41 0.40702536597653705 1105 42-43 0.4308609487698476 1105 44-45 0.46107996368358073 1105 46-47 0.47606948520432724 1105 48-49 0.49447491006542066 1105 50-51 0.5529684790440612 1105 52-53 0.5527309081737641 1105 54-55 0.5314421776419351 1105 56-57 0.5385491523641477 1105 58-59 0.5531535654962028 1105 60-61 0.5425785894321749 1105 62-63 0.5339158181261467 1105 64-65 0.5729288724307438 1105 66-67 0.5451375215475167 1105 68-69 0.5786553319055869 1105 70-71 0.5410078289062099 1105 72-73 0.5417935510289524 1105 74-75 0.5419056609897197 1105 76-77 0.49440106708225073 1105 78-79 0.4380997540851226 1105 80-81 0.452055678116956 1105 82-83 0.47981792445701643 1105 84-85 0.5104343153841668 1105 86-87 0.5678049482314051 1105 88-89 0.5327302082589327 1105 90-91 0.46612914374914993 1105 92-93 0.5136168343188992 1105 94-95 0.5407041057485422 1105 96-97 0.5809708094364687 1105 98-99 0.5831445805142614 1105 100 0.6204479039778938 1106 1 0.13936674107874936 1106 2 0.14941255449572566 1106 3 0.2708112101192697 1106 4 0.20211115777811983 1106 5 0.15302491322326262 1106 6 0.20481433444475527 1106 7 0.19604482756204789 1106 8 0.20267023400619166 1106 9 0.20829433678281362 1106 10-11 0.20589491220574274 1106 12-13 0.20317191997570205 1106 14-15 0.2529476169065532 1106 16-17 0.25922802605860795 1106 18-19 0.24957127852061944 1106 20-21 0.22412501295460174 1106 22-23 0.23669940508509058 1106 24-25 0.22155169858742596 1106 26-27 0.22208734372763672 1106 28-29 0.22532835058723322 1106 30-31 0.252566391569232 1106 32-33 0.26951426538315104 1106 34-35 0.25414186583319065 1106 36-37 0.2775399630815585 1106 38-39 0.25774007286717904 1106 40-41 0.2609344387734609 1106 42-43 0.2881813494267149 1106 44-45 0.271747980276146 1106 46-47 0.3224732024724659 1106 48-49 0.37506092599197416 1106 50-51 0.4197268576879054 1106 52-53 0.38064986182738636 1106 54-55 0.32223639835660833 1106 56-57 0.34006848073487106 1106 58-59 0.3330476853777853 1106 60-61 0.36830042242695527 1106 62-63 0.3876256462949641 1106 64-65 0.35281208631316474 1106 66-67 0.3782889654209711 1106 68-69 0.35575341686364226 1106 70-71 0.387145878324084 1106 72-73 0.4055110337855581 1106 74-75 0.40027474375190053 1106 76-77 0.3262868105555441 1106 78-79 0.3696506951880778 1106 80-81 0.2946625660298281 1106 82-83 0.31915222212533934 1106 84-85 0.3072409644309744 1106 86-87 0.35576840580156244 1106 88-89 0.32597642236776636 1106 90-91 0.3097658956428617 1106 92-93 0.3118166219219738 1106 94-95 0.3137378048924795 1106 96-97 0.3613288474153826 1106 98-99 0.3847205599885193 1106 100 0.3699617339222847 1107 1 0.1986892232982278 1107 2 0.15243829524519725 1107 3 0.24214587963286505 1107 4 0.19034714848426404 1107 5 0.1688323668543461 1107 6 0.15127675633490867 1107 7 0.14828301165695734 1107 8 0.17750318954143296 1107 9 0.18770120386705003 1107 10-11 0.1849711006460666 1107 12-13 0.15537395072628613 1107 14-15 0.19989862591872054 1107 16-17 0.16667354291649872 1107 18-19 0.20569851111060444 1107 20-21 0.1993660574143803 1107 22-23 0.20242871875418444 1107 24-25 0.2369028402679092 1107 26-27 0.23601678342829757 1107 28-29 0.25532182996518316 1107 30-31 0.24609738681130722 1107 32-33 0.23229043045834885 1107 34-35 0.20732382453944354 1107 36-37 0.23340303082605374 1107 38-39 0.23731905563204236 1107 40-41 0.23064043580875193 1107 42-43 0.24084084095827762 1107 44-45 0.2485681721803914 1107 46-47 0.302566377351873 1107 48-49 0.36094442189038034 1107 50-51 0.39775822666191374 1107 52-53 0.295528607235525 1107 54-55 0.2549600411343036 1107 56-57 0.23960905580204894 1107 58-59 0.2543244273816825 1107 60-61 0.18484112088547278 1107 62-63 0.1860698813784083 1107 64-65 0.228838400906902 1107 66-67 0.20262844329803187 1107 68-69 0.21803599148640984 1107 70-71 0.1952009952237006 1107 72-73 0.24310290035602833 1107 74-75 0.25098306801121595 1107 76-77 0.19720694307292774 1107 78-79 0.18462994863408255 1107 80-81 0.1889856129864711 1107 82-83 0.21111189867986724 1107 84-85 0.2204837858041273 1107 86-87 0.2618974639874807 1107 88-89 0.2594762985099237 1107 90-91 0.22651038686535685 1107 92-93 0.2116588067428289 1107 94-95 0.20569250318197518 1107 96-97 0.19300977621955084 1107 98-99 0.29789177093386954 1107 100 0.28492516901817666 1108 1 0.085537453186312 1108 2 0.13224353982881354 1108 3 0.19048652641738784 1108 4 0.12693086641450435 1108 5 0.10630266406048605 1108 6 0.1571088825129081 1108 7 0.1268442026844525 1108 8 0.15295708788580242 1108 9 0.12294569802148914 1108 10-11 0.13266201377263798 1108 12-13 0.09967026857427186 1108 14-15 0.12664887817464887 1108 16-17 0.17955766109378857 1108 18-19 0.16938630288836265 1108 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-0.5946831779863686 2215 18-19 -0.6192448287926737 2215 20-21 -0.671157435558456 2215 22-23 -0.6705655259261931 2215 24-25 -0.6742417760451929 2215 26-27 -0.6117541470588819 2215 28-29 -0.5824067315153769 2215 30-31 -0.5416988896337074 2215 32-33 -0.6066564594313775 2215 34-35 -0.6850637360127578 2215 36-37 -0.7143474421036586 2215 38-39 -0.6799905805189326 2215 40-41 -0.6932988515664462 2215 42-43 -0.6564824796938851 2215 44-45 -0.8134670279709866 2215 46-47 -0.7638998075809482 2215 48-49 -0.7284847910424759 2215 50-51 -0.7056112225254054 2215 52-53 -0.7990953889977064 2215 54-55 -0.7558447662145085 2215 56-57 -0.7098857252621542 2215 58-59 -0.6743903188959379 2215 60-61 -0.826429266229816 2215 62-63 -0.818593631233874 2215 64-65 -0.708780929645549 2215 66-67 -0.6299256782596387 2215 68-69 -0.5487775994346222 2215 70-71 -0.6871298458332262 2215 72-73 -0.555059046006491 2215 74-75 -0.691642118538887 2215 76-77 -0.6597232772642272 2215 78-79 -0.6138883297205737 2215 80-81 -0.6491851369640713 2215 82-83 -0.5734914189983726 2215 84-85 -0.5471873435234258 2215 86-87 -0.5645151132599509 2215 88-89 -0.5527541868345835 2215 90-91 -0.5832227609315481 2215 92-93 -0.7035251445591513 2215 94-95 -0.7313293978100326 2215 96-97 -0.7920908601385186 2215 98-99 -0.7684793888067354 2215 100 -0.9324885711928559 2216 1 -0.6718982199034897 2216 2 -1.1267429087164125 2216 3 -0.7227765411845546 2216 4 -1.145803702759423 2216 5 -0.6213794846346516 2216 6 -0.8449482774546482 2216 7 -0.5391677371106738 2216 8 -0.5817173763214925 2216 9 -0.6195508760843822 2216 10-11 -0.8807582866081134 2216 12-13 -0.6994295077757471 2216 14-15 -0.9797586329638079 2216 16-17 -0.940380675966864 2216 18-19 -0.9333306686974723 2216 20-21 -0.7703615109175814 2216 22-23 -0.7664650217933939 2216 24-25 -0.8389666708136829 2216 26-27 -0.9624915765787136 2216 28-29 -1.2516203371299923 2216 30-31 -1.333758123985163 2216 32-33 -1.0211929833458058 2216 34-35 -1.0618678010480878 2216 36-37 -1.049078982819303 2216 38-39 -1.2244446093631751 2216 40-41 -1.0495122295181147 2216 42-43 -1.598917680505302 2216 44-45 -1.2773473982256505 2216 46-47 -1.1155061169259568 2216 48-49 -1.134946648063213 2216 50-51 -1.2498233354261856 2216 52-53 -1.169034607616915 2216 54-55 -1.298232662443354 2216 56-57 -1.4011879803578964 2216 58-59 -1.2121435794644455 2216 60-61 -1.45362524087178 2216 62-63 -1.4097501011563622 2216 64-65 -1.3976848510721709 2216 66-67 -1.2221643897798913 2216 68-69 -1.5438989291402407 2216 70-71 -1.311462554602258 2216 72-73 -1.1531798204225723 2216 74-75 -1.2555279588674395 2216 76-77 -1.3358219290836217 2216 78-79 -1.3977905812337994 2216 80-81 -1.1720162189639467 2216 82-83 -1.281608806398637 2216 84-85 -1.3407005292906007 2216 86-87 -1.447131396629807 2216 88-89 -1.3481858950808743 2216 90-91 -1.159098728739778 2216 92-93 -1.2761640179858809 2216 94-95 -1.3701519518876815 2216 96-97 -1.4405656147845178 2216 98-99 -1.4910449459087225 2216 100 -1.4756471651989642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 530.0 3 53.0 4 66.0 5 173.0 6 399.0 7 1061.0 8 2370.0 9 4008.0 10 5954.0 11 8423.0 12 12424.0 13 17167.0 14 20734.0 15 26771.0 16 35162.0 17 49274.0 18 50150.0 19 27544.0 20 20208.0 21 19401.0 22 20597.0 23 22509.0 24 25637.0 25 32124.0 26 41016.0 27 53188.0 28 70419.0 29 92634.0 30 120600.0 31 154684.0 32 200292.0 33 260429.0 34 353551.0 35 513837.0 36 839591.0 37 1547732.0 38 2208953.0 39 919163.0 40 3213.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.38091847875213 20.625815758299858 14.805086564403744 23.18817919854427 2 15.502625221167124 20.59341730562961 39.970298165859695 23.933659307343564 3 17.969123255373464 25.19891318957836 30.973493061843183 25.858470493204994 4 12.59076996707695 17.430239544455123 38.222386901798224 31.7566035866697 5 12.557775182577801 37.65068322751497 35.62623877393249 14.165302815974734 6 30.292563136813456 38.313010559104384 17.307010746718994 14.087415557363167 7 26.20195653042692 33.1575868078824 22.428563406437977 18.211893255252704 8 24.708376632813938 36.29912769670579 20.33947135462278 18.653024315857497 9 24.485272943318055 16.767512156521487 21.958402408007053 36.788812492153404 10-11 23.571733944860995 27.213889004182835 28.701307279157227 20.513069771798943 12-13 24.400541066457073 24.68526723428572 29.13985840599478 21.774333293262426 14-15 21.58470935445068 26.719469023478293 27.027177581608107 24.668644040462915 16-17 20.44786194264461 30.027469657381655 27.814104294747356 21.71056410522638 18-19 20.304159281607486 28.19109408444391 30.88518808883171 20.61955854511689 20-21 21.302682676691116 27.440757508216674 30.82294220757768 20.43361760751453 22-23 21.010330066366908 27.28982666629487 30.94341188898902 20.756431378349202 24-25 20.899112456487956 27.338310091144468 30.88872829120278 20.873849161164788 26-27 20.674404097485745 27.35487562677167 30.94426198373036 21.02645829201223 28-29 20.735435497312814 27.398942319473647 31.04684080763775 20.81878137557579 30-31 21.03145185550129 27.699893125449034 30.604665548962153 20.663989470087525 32-33 20.710163493141064 27.491342564590077 30.957574229581734 20.840919712687125 34-35 20.855216517106502 27.2880790527832 30.88056847811519 20.97613595199511 36-37 21.202618953048436 27.480239438471223 30.42855980841016 20.888581800070188 38-39 21.01723056971815 27.63758247996894 30.532350060864495 20.81283688944841 40-41 20.933357971257156 27.459223100983404 30.583686207769915 21.023732719989525 42-43 20.951662937782004 27.52408397745527 30.3733622066499 21.150890878112826 44-45 21.14705512345669 27.645832243751993 30.138263213982043 21.068849418809283 46-47 21.227785872626473 27.84305556858413 29.734416716642848 21.194741842146552 48-49 21.274881486746214 27.83909131293449 29.46288769231619 21.423139508003107 50-51 21.333124562052554 27.888435694440574 29.242823315888465 21.535616427618407 52-53 21.3165607839897 27.999203550842257 29.185691774175954 21.498543890992096 54-55 21.385880644936208 28.238999254822737 29.040427826067738 21.334692274173317 56-57 21.251256579090242 28.013550686767136 29.096634160627012 21.638558573515613 58-59 21.15582346631389 27.696349055564244 29.42382303326479 21.724004444857076 60-61 21.341746978716767 27.9262856620776 29.143344271766235 21.5886230874394 62-63 21.10809362222584 27.973882430072006 29.252615091593583 21.66540885610857 64-65 21.127426596698733 27.76009532717702 29.373708002823424 21.738770073300824 66-67 21.07272372376347 27.7542292568235 29.25612316871628 21.91692385069675 68-69 21.431995980488406 27.921791715282886 29.0136262471023 21.63258605712641 70-71 21.34059689482489 27.71831322913873 29.078316344002815 21.862773532033565 72-73 21.21280265678374 27.811849102311335 29.16818993886051 21.807158302044414 74-75 21.291284638567184 27.887696813727914 28.952533146510024 21.868485401194878 76-77 21.28536074276658 27.90241017748429 28.83619477203988 21.97603430770925 78-79 21.335368620351165 27.897781440346357 28.957665421790274 21.809184517512204 80-81 21.221333592392103 27.83775365207328 28.952265337859668 21.988647417674954 82-83 21.108629462758614 27.792953083867495 29.121105410501702 21.977312042872192 84-85 21.158890064958538 27.71488225261214 28.911759781270753 22.214467901158578 86-87 21.2189720678015 27.82825404211145 28.85858792433681 22.094185965750242 88-89 21.09155860531384 27.678573895332683 28.902822686218098 22.327044813135377 90-91 20.9456585116687 27.61537707830372 28.91195268591291 22.52701172411467 92-93 21.023831292618407 27.740444449013395 28.793381388705598 22.4423428696626 94-95 20.873572352362125 27.535558552932837 28.92904557748961 22.661823517215428 96-97 20.84857435527353 27.550621202755245 28.905227362926215 22.695577079045016 98-99 20.838172468445926 27.52812309577517 28.868331149243936 22.765373286534967 100 20.891909552291168 27.488889360064405 28.764391467182733 22.854809620461698 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 842.0 1 714.5 2 1294.0 3 2037.5 4 1359.5 5 812.5 6 1995.0 7 5193.5 8 8679.0 9 9958.5 10 7870.0 11 5219.0 12 4763.5 13 5317.0 14 6088.5 15 6844.0 16 7708.0 17 8661.0 18 9828.0 19 11264.5 20 13241.5 21 16037.5 22 20394.5 23 26937.5 24 35330.0 25 45662.5 26 59982.5 27 78209.0 28 101320.5 29 125305.5 30 146047.5 31 166747.0 32 185737.5 33 203423.0 34 221416.0 35 237224.5 36 248951.0 37 258999.0 38 268405.5 39 275096.0 40 278581.5 41 278461.0 42 273811.0 43 269510.5 44 265762.0 45 260140.0 46 254447.0 47 246654.0 48 237683.0 49 234119.0 50 230991.5 51 221647.5 52 209447.0 53 199830.0 54 191966.5 55 180523.5 56 166351.0 57 149130.0 58 133012.5 59 121112.5 60 106246.5 61 88752.0 62 74955.0 63 61975.5 64 49167.5 65 38922.5 66 30410.5 67 23305.5 68 17740.5 69 13375.0 70 9700.5 71 7046.0 72 5425.5 73 3978.0 74 2796.5 75 1881.5 76 1107.0 77 662.5 78 401.0 79 241.0 80 134.5 81 64.0 82 31.5 83 10.0 84 6.5 85 6.0 86 4.0 87 3.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 1.0 98 1.0 99 1.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06126927370338964 2 3.8550298051629383E-4 3 3.8550298051629386E-5 4 0.12041828101393966 5 0.0019660652006330986 6 0.0016833630149211498 7 0.0 8 0.0 9 5.140039740217251E-5 10-11 0.0 12-13 3.2125248376357826E-5 14-15 2.5700198701086256E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.425049675271564E-6 28-29 1.606262418817891E-4 30-31 2.570019870108626E-4 32-33 2.5700198701086256E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.7347634123233223E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.2850099350543128E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.285009935054313E-4 80-81 2.95552285062492E-4 82-83 3.4695268246466446E-4 84-85 0.0 86-87 6.425049675271564E-6 88-89 4.4975347726900946E-5 90-91 4.4975347726900946E-5 92-93 5.91104570124984E-4 94-95 6.746302159035142E-4 96-97 6.425049675271564E-6 98-99 3.8550298051629386E-5 100 0.001105108544146709 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7782041.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.87682412326953 #Duplication Level Percentage of deduplicated Percentage of total 1 73.93226760549234 42.05032581632086 2 15.25604859446995 17.354311854474414 3 4.819809971033792 8.224064520902093 4 2.0874060880696446 4.74900115779917 5 1.1204746046993863 3.1864518513038473 6 0.6449157747633694 2.200845665732296 7 0.42673016898610105 1.698973973866089 8 0.2883169049206565 1.311883991435008 9 0.22441957721430034 1.1487845540732642 >10 1.1049951570410514 11.098101110439304 >50 0.06559939547860084 2.5254355623614435 >100 0.027366244907493297 2.7154990397507692 >500 0.0010623825073451253 0.3963176489181793 >1k 4.971411345003011E-4 0.4581451912803184 >5k 2.259732034530787E-5 0.0881562450665464 >10k+ 6.779196103592361E-5 0.7937018162763533 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24389 0.3134010730603964 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23584 0.3030567430832091 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13781 0.17708721914983486 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.2850099350543128E-5 0.0 5 0.0 6.425049675271564E-5 0.0 1.2850099350543128E-5 0.0 6 0.0 6.425049675271564E-5 0.0 1.2850099350543128E-5 0.0 7 0.0 6.425049675271564E-5 0.0 1.2850099350543128E-5 0.0 8 0.0 6.425049675271564E-5 0.0 1.2850099350543128E-5 0.0 9 1.2850099350543128E-5 6.425049675271564E-5 0.0 3.8550298051629386E-5 0.0 10-11 1.2850099350543128E-5 6.425049675271564E-5 0.0 3.8550298051629386E-5 0.0 12-13 1.2850099350543128E-5 7.06755464279872E-5 0.0 5.140039740217251E-5 0.0 14-15 1.2850099350543128E-5 8.99506954538019E-5 0.0 7.710059610325877E-5 0.0 16-17 1.2850099350543128E-5 1.2207594383015972E-4 0.0 1.0280079480434504E-4 0.0 18-19 1.2850099350543128E-5 1.47776142531246E-4 0.0 1.1565089415488816E-4 0.0 20-21 1.2850099350543128E-5 1.799013909076038E-4 0.0 1.6705129155706065E-4 0.0 22-23 1.2850099350543128E-5 1.8632644058287536E-4 0.0 2.6342703668613416E-4 0.0 24-25 1.2850099350543128E-5 1.9275149025814694E-4 0.0 3.8550298051629383E-4 0.0 26-27 1.2850099350543128E-5 1.9275149025814694E-4 0.0 7.196055636304152E-4 0.0 28-29 1.2850099350543128E-5 2.0560158960869005E-4 0.0 0.0024993443236806384 0.0 30-31 1.2850099350543128E-5 2.0560158960869005E-4 0.0 0.008185513286295973 0.0 32-33 1.2850099350543128E-5 2.0560158960869005E-4 0.0 0.021241214226447792 0.0 34-35 1.2850099350543128E-5 2.1845168895923318E-4 0.0 0.042623779545751556 0.0 36-37 1.2850099350543128E-5 2.3130178830977632E-4 0.0 0.07707489590455768 0.0 38-39 1.2850099350543128E-5 2.4415188766031945E-4 0.0 0.12350872990774528 0.0 40-41 1.2850099350543128E-5 2.4415188766031945E-4 0.0 0.17285311141383092 0.0 42-43 1.2850099350543128E-5 2.4415188766031945E-4 0.0 0.22354675335172353 0.0 44-45 1.2850099350543128E-5 2.50576937335591E-4 0.0 0.2882277284326824 0.0 46-47 1.2850099350543128E-5 2.5700198701086256E-4 0.0 0.36890707720506744 0.0 48-49 1.2850099350543128E-5 2.5700198701086256E-4 0.0 0.45630060288811125 0.0 50-51 1.2850099350543128E-5 2.698520863614057E-4 0.0 0.5461356474477583 0.0 52-53 1.2850099350543128E-5 2.95552285062492E-4 0.0 0.6370950757005778 0.0 54-55 1.2850099350543128E-5 2.95552285062492E-4 0.0 0.7443805551782623 0.0 56-57 1.2850099350543128E-5 2.95552285062492E-4 0.0 0.8742243840658254 0.0 58-59 1.2850099350543128E-5 2.95552285062492E-4 0.0 1.0200858617938404 0.0 60-61 1.2850099350543128E-5 2.95552285062492E-4 0.0 1.1716399335341463 0.0 62-63 1.2850099350543128E-5 2.95552285062492E-4 0.0 1.3265992816023457 0.0 64-65 1.2850099350543128E-5 2.95552285062492E-4 0.0 1.4974606276168423 0.0 66-67 1.2850099350543128E-5 3.212524837635782E-4 0.0 1.7005307476534754 0.0 68-69 1.2850099350543128E-5 3.212524837635782E-4 0.0 1.9301106226502789 1.2850099350543128E-5 70-71 1.2850099350543128E-5 3.212524837635782E-4 0.0 2.179004196970949 1.2850099350543128E-5 72-73 1.2850099350543128E-5 3.212524837635782E-4 0.0 2.443041613376234 1.2850099350543128E-5 74-75 1.2850099350543128E-5 3.212524837635782E-4 0.0 2.7139859581824357 1.2850099350543128E-5 76-77 1.2850099350543128E-5 3.212524837635782E-4 0.0 3.013412548199116 1.2850099350543128E-5 78-79 1.2850099350543128E-5 3.3410258311412135E-4 0.0 3.3577052600982187 1.2850099350543128E-5 80-81 1.2850099350543128E-5 3.3410258311412135E-4 0.0 3.7335835162009556 1.2850099350543128E-5 82-83 1.2850099350543128E-5 3.3410258311412135E-4 0.0 4.121238888358466 1.2850099350543128E-5 84-85 1.2850099350543128E-5 3.4695268246466446E-4 0.0 4.51172770742277 1.2850099350543128E-5 86-87 1.2850099350543128E-5 3.4695268246466446E-4 0.0 4.936808993938737 1.2850099350543128E-5 88 1.2850099350543128E-5 3.4695268246466446E-4 0.0 5.286171070031628 1.2850099350543128E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14080 0.0 58.102657 1 GTATCAA 25680 0.0 41.9932 1 ATCAACG 32110 0.0 33.110912 3 TCAACGC 32285 0.0 32.96058 4 TATCAAC 33040 0.0 32.264557 2 CAACGCA 33475 0.0 31.814594 5 GTGGTAT 5525 0.0 30.670378 1 AACGCAG 35110 0.0 30.359043 6 TGGTATC 6120 0.0 26.673738 2 ACGCAGA 39780 0.0 26.641438 7 CGCAGAG 39930 0.0 26.423658 8 GCAGAGT 42800 0.0 24.234522 9 GTACATG 20710 0.0 22.978409 1 TACATGG 20945 0.0 21.989138 2 GAGTACT 31665 0.0 21.743729 12-13 CAGAGTA 41880 0.0 21.490078 10-11 AGAGTAC 39995 0.0 21.086945 10-11 ACATGGG 21135 0.0 20.722897 3 GTACTTT 34820 0.0 19.611588 14-15 AGTACTT 33280 0.0 19.474075 12-13 >>END_MODULE