FastQCFastQC Report
Wed 25 May 2016
SRR2049515_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049515_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8244883
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71600.041.789611
GTATCAA118650.035.193171
ATCAACG120500.033.116493
TCAACGC120000.032.9981164
CAACGCA136850.029.1965665
TATCAAC143300.028.2049082
AACGCAG147150.027.082916
GTACATG191350.023.5893481
ACGCAGA166150.023.5539117
CGCAGAG168500.022.971758
TACATGG192400.022.3915842
ACATGGG202250.020.541223
GCAGAGT198700.018.4107259
CATGGGG146400.017.4952374
GTGGTAT48350.017.1667081
AGAGTAC167150.016.57000210-11
GAGTACA149450.016.0442121
GTATTAG37300.015.6590181
CAGAGTA191600.015.17533510-11
TATACTG47700.014.92725
Produced by FastQC (version 0.11.5)