##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049515_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8244883 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.186008825110072 31.0 30.0 34.0 26.0 34.0 2 30.45293632426318 31.0 31.0 34.0 27.0 34.0 3 30.528020470393574 31.0 31.0 34.0 27.0 34.0 4 34.1203101366023 37.0 35.0 37.0 30.0 37.0 5 34.07237676993112 37.0 35.0 37.0 30.0 37.0 6 34.11961334078361 37.0 35.0 37.0 30.0 37.0 7 30.268754450487656 36.0 32.0 37.0 2.0 37.0 8 32.0318705553493 37.0 32.0 37.0 17.0 37.0 9 34.69994128479446 38.0 33.0 39.0 27.0 39.0 10-11 35.223549685301776 38.5 35.0 39.0 28.5 39.0 12-13 35.355174051590545 39.0 35.0 39.0 29.0 39.0 14-15 36.4669418595752 40.0 36.0 41.0 29.0 41.0 16-17 36.4717818918716 40.0 36.0 41.0 29.0 41.0 18-19 36.465120730033405 40.0 36.5 41.0 29.0 41.0 20-21 36.51045066376321 40.0 37.0 41.0 29.0 41.0 22-23 36.46561606756579 40.0 36.0 41.0 29.0 41.0 24-25 36.438104276312956 40.0 36.0 41.0 29.0 41.0 26-27 36.4368886738599 40.0 36.0 41.0 29.0 41.0 28-29 36.41279609425628 40.0 36.0 41.0 29.0 41.0 30-31 36.32854420129431 40.0 36.0 41.0 29.0 41.0 32-33 36.271526412200146 40.0 36.0 41.0 29.0 41.0 34-35 36.2066447152737 40.0 36.0 41.0 29.0 41.0 36-37 36.05871011147156 40.0 36.0 41.0 28.5 41.0 38-39 35.94285261537368 40.0 35.5 41.0 28.0 41.0 40-41 35.84931629715061 40.0 35.0 41.0 27.5 41.0 42-43 35.72866722305216 39.5 35.0 41.0 27.0 41.0 44-45 35.60782675751736 39.0 35.0 41.0 27.0 41.0 46-47 35.45693813969222 39.0 35.0 41.0 27.0 41.0 48-49 35.378480567886776 39.0 35.0 41.0 26.5 41.0 50-51 35.25751687440562 39.0 35.0 41.0 26.0 41.0 52-53 35.1357990161898 39.0 35.0 41.0 26.0 41.0 54-55 35.01089506060911 39.0 34.0 40.5 26.0 41.0 56-57 34.814024468267164 39.0 34.0 40.0 25.0 41.0 58-59 34.63283808878792 38.5 34.0 40.0 25.0 41.0 60-61 34.42003834378244 38.0 34.0 40.0 24.0 41.0 62-63 34.190384569435366 38.0 34.0 40.0 23.0 41.0 64-65 33.852456365966624 37.5 33.0 40.0 22.5 41.0 66-67 30.494829338390854 36.0 29.0 40.0 6.5 41.0 68-69 30.832952026123355 36.0 28.5 39.0 7.5 41.0 70-71 30.98992908692579 35.0 28.0 39.0 11.5 41.0 72-73 32.164256363613646 35.0 31.5 39.0 18.0 40.5 74-75 32.22513915600743 35.0 32.0 38.5 18.0 40.0 76-77 31.91580917521813 35.0 32.0 37.5 16.0 39.5 78-79 28.609115193023356 35.0 26.0 37.0 5.0 39.0 80-81 30.320549060550647 35.0 28.5 36.0 8.0 39.0 82-83 30.68548819916547 35.0 31.0 36.0 7.0 38.5 84-85 28.91775783840717 34.5 28.5 36.0 3.5 37.0 86-87 28.54506200997637 34.0 26.0 35.0 2.0 37.0 88-89 29.6799844824966 34.0 30.0 35.0 2.0 36.5 90-91 29.799405158326685 34.0 30.5 35.0 2.0 36.0 92-93 29.634667344582088 34.0 31.0 35.0 2.0 36.0 94-95 29.4128460040003 34.0 31.0 35.0 2.0 36.0 96-97 29.209977630974265 34.0 30.5 35.0 2.0 35.5 98-99 28.993052478731354 34.0 30.0 35.0 2.0 35.0 100 28.772867243840818 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4415781621746788 1101 2 0.5016929272910673 1101 3 0.4969124458936598 1101 4 0.4936940709279156 1101 5 0.4653018585409612 1101 6 0.5001336401724572 1101 7 -11.807545137273848 1101 8 -6.237603758885914 1101 9 -1.9415512083037285 1101 10-11 0.06034049200880531 1101 12-13 0.543411905515292 1101 14-15 0.708753867388964 1101 16-17 0.78089086664734 1101 18-19 0.765568749199872 1101 20-21 0.7451369493043742 1101 22-23 0.7719505717796267 1101 24-25 0.8058830367616139 1101 26-27 0.7610775861217007 1101 28-29 0.7306581117145043 1101 30-31 0.7861547535751541 1101 32-33 0.8368172088326631 1101 34-35 0.8254286469952987 1101 36-37 0.8685928692808815 1101 38-39 0.8897839829909628 1101 40-41 0.8941148509050691 1101 42-43 0.8424017753406545 1101 44-45 0.7318260458681678 1101 46-47 0.7394146330447455 1101 48-49 0.6855950159413524 1101 50-51 0.7692417672467116 1101 52-53 0.7955219316939335 1101 54-55 0.7602455974887405 1101 56-57 0.7080665413573541 1101 58-59 0.7230306449230923 1101 60-61 0.7173353483072091 1101 62-63 0.710559285827145 1101 64-65 0.59762229394304 1101 66-67 -9.790228715750953 1101 68-69 -7.709016467960325 1101 70-71 -6.250916331328639 1101 72-73 -1.2593248928066103 1101 74-75 -0.0024800886518789866 1101 76-77 0.2872338731044266 1101 78-79 3.3534321649712595 1101 80-81 1.3002349509378028 1101 82-83 0.6766467462686663 1101 84-85 -5.00242102701128 1101 86-87 -5.445055647847187 1101 88-89 -1.0649524710329459 1101 90-91 0.20099631008006824 1101 92-93 0.5668807597685763 1101 94-95 0.6153936836793221 1101 96-97 0.7488951454700619 1101 98-99 0.7700333948194427 1101 100 0.8444744567132361 1102 1 0.26453591329536863 1102 2 0.34103005477238924 1102 3 0.31577549225135115 1102 4 0.31755793707971947 1102 5 0.307254153334938 1102 6 0.3738218130581217 1102 7 -11.857393084902249 1102 8 -6.39529105053591 1102 9 -2.1225342621995296 1102 10-11 -0.10801153329548185 1102 12-13 0.4080450619249234 1102 14-15 0.6263822343157344 1102 16-17 0.6608683431222175 1102 18-19 0.6016138889972993 1102 20-21 0.6146850277351774 1102 22-23 0.6077761526173475 1102 24-25 0.6498288554916911 1102 26-27 0.6138460614704613 1102 28-29 0.6215685799452686 1102 30-31 0.5981136129143678 1102 32-33 0.6148279603482152 1102 34-35 0.609425560606347 1102 36-37 0.6929468539722308 1102 38-39 0.6611888658539797 1102 40-41 0.5935343314012229 1102 42-43 0.5749304352810398 1102 44-45 0.5936936820408505 1102 46-47 0.5997786126879774 1102 48-49 0.4876353950846095 1102 50-51 0.546620018187923 1102 52-53 0.5429670018759438 1102 54-55 0.5118388981436013 1102 56-57 0.5861819292885357 1102 58-59 0.577712030546472 1102 60-61 0.4715844894842789 1102 62-63 0.49455238084200914 1102 64-65 0.38351795806124045 1102 66-67 -9.98290774444045 1102 68-69 -6.477770457275273 1102 70-71 -5.995106213397317 1102 72-73 -0.9997250142586118 1102 74-75 -0.08385637882996377 1102 76-77 0.09389622945898424 1102 78-79 3.164317169701846 1102 80-81 1.1410507730261727 1102 82-83 0.5140414157973794 1102 84-85 -5.20985805612419 1102 86-87 -5.672620328846136 1102 88-89 -1.279176002158323 1102 90-91 -0.0501855316763411 1102 92-93 0.34548584309757047 1102 94-95 0.3926810313904383 1102 96-97 0.5123352898694158 1102 98-99 0.5576490681820943 1102 100 0.5992686010095234 1103 1 0.37260917578808517 1103 2 0.39953389060287847 1103 3 0.40104013195993105 1103 4 0.43225908049904405 1103 5 0.3812774254408069 1103 6 0.43737823002876297 1103 7 -11.75125602084886 1103 8 -6.274392549890123 1103 9 -2.021264266921726 1103 10-11 -0.07125152692862002 1103 12-13 0.487313260982404 1103 14-15 0.6693842055914345 1103 16-17 0.6932400852805429 1103 18-19 0.7110332577985972 1103 20-21 0.7482697480817393 1103 22-23 0.7186346817352671 1103 24-25 0.8097590947682249 1103 26-27 0.7716458868121947 1103 28-29 0.6966931786664077 1103 30-31 0.6862558121459159 1103 32-33 0.7152556877952776 1103 34-35 0.7351233746644468 1103 36-37 0.806275103309325 1103 38-39 0.7914730313511242 1103 40-41 0.8048401121001945 1103 42-43 0.7784379624965183 1103 44-45 0.7289919264241078 1103 46-47 0.7762843453377286 1103 48-49 0.7479255402614342 1103 50-51 0.6792109601148795 1103 52-53 0.6484437034621422 1103 54-55 0.6980416890358256 1103 56-57 0.6554213087387595 1103 58-59 0.6707390191870601 1103 60-61 0.6367707249036414 1103 62-63 0.5464105158371595 1103 64-65 0.430445322657242 1103 66-67 -9.857693086514857 1103 68-69 -7.856134341466053 1103 70-71 -6.413253730473489 1103 72-73 -1.4065543908662512 1103 74-75 -0.2045540290838943 1103 76-77 0.11744240122305172 1103 78-79 3.1756970428346847 1103 80-81 1.118826831871253 1103 82-83 0.4893125282527464 1103 84-85 -5.150830146209209 1103 86-87 -5.611649751054209 1103 88-89 -1.2275461671637302 1103 90-91 0.024286497685309882 1103 92-93 0.39616177523432583 1103 94-95 0.49141312089303923 1103 96-97 0.6080486007952253 1103 98-99 0.628402511130755 1103 100 0.687074687310826 1104 1 0.20153097385977148 1104 2 0.24150110076014286 1104 3 0.24771916630597346 1104 4 0.21271814495064234 1104 5 0.22234000394298192 1104 6 0.27513450093449165 1104 7 -11.952528183503944 1104 8 -6.437920871800195 1104 9 -2.2468358455991577 1104 10-11 -0.30008018515994905 1104 12-13 0.2938181662417776 1104 14-15 0.5128206984897261 1104 16-17 0.48331556795925934 1104 18-19 0.49133116838326174 1104 20-21 0.5123189084397382 1104 22-23 0.482308842121256 1104 24-25 0.6367634781751548 1104 26-27 0.5687569076274599 1104 28-29 0.5141587955655851 1104 30-31 0.4785180594927425 1104 32-33 0.46693036726682635 1104 34-35 0.5114987698682327 1104 36-37 0.5613061596788214 1104 38-39 0.5742590308446438 1104 40-41 0.6020135332178853 1104 42-43 0.5568197698847541 1104 44-45 0.5750412288684998 1104 46-47 0.5122326963886223 1104 48-49 0.45929670629975305 1104 50-51 0.5384301681741803 1104 52-53 0.5093788430690651 1104 54-55 0.48609698591402406 1104 56-57 0.5028996254493379 1104 58-59 0.47732397199451526 1104 60-61 0.4611425819578159 1104 62-63 0.4316789105478023 1104 64-65 0.31758880679161905 1104 66-67 -9.985248123482432 1104 68-69 -8.022981999290788 1104 70-71 -6.551042125955654 1104 72-73 -1.5903840408555112 1104 74-75 -0.35068129352187327 1104 76-77 -0.04473366570201165 1104 78-79 3.0572422525543033 1104 80-81 1.018596090215766 1104 82-83 0.3694322262862073 1104 84-85 -5.232234747332676 1104 86-87 -5.656119457079626 1104 88-89 -1.325598614317233 1104 90-91 -0.04455184430014114 1104 92-93 0.3093804930603703 1104 94-95 0.3872789217304522 1104 96-97 0.49147904622520144 1104 98-99 0.5534948696878565 1104 100 0.642829898102331 1105 1 0.06436979849549118 1105 2 0.07125011503445933 1105 3 0.055747378246611135 1105 4 0.1253122934324935 1105 5 0.08346440524952925 1105 6 0.1413027036992247 1105 7 -12.000880440045634 1105 8 -6.5216212307814665 1105 9 -2.4403807676606775 1105 10-11 -0.42040770833882846 1105 12-13 0.10762789578402021 1105 14-15 0.2510673386712412 1105 16-17 0.33479223174647643 1105 18-19 0.3408928785503633 1105 20-21 0.3390713999466115 1105 22-23 0.327645486245018 1105 24-25 0.3257778458509222 1105 26-27 0.31041061615984944 1105 28-29 0.3148967051286533 1105 30-31 0.3195189296269447 1105 32-33 0.3323375319431392 1105 34-35 0.340087427488875 1105 36-37 0.38399463488925534 1105 38-39 0.3737425520523985 1105 40-41 0.3507798894288783 1105 42-43 0.2636215903429573 1105 44-45 0.23436426223820916 1105 46-47 0.2982788623957049 1105 48-49 0.303936531374994 1105 50-51 0.35049347335588266 1105 52-53 0.30865118660668855 1105 54-55 0.2607592096654514 1105 56-57 0.21634356621678563 1105 58-59 0.22709728200730694 1105 60-61 0.16946543710248108 1105 62-63 0.1586289634781437 1105 64-65 -0.014907773756831944 1105 66-67 -10.101237535517786 1105 68-69 -8.200592345024614 1105 70-71 -6.644433779071218 1105 72-73 -1.8243811554684441 1105 74-75 -0.6785776948137112 1105 76-77 -0.3413010665786764 1105 78-79 2.7169413166406855 1105 80-81 0.6817146864409906 1105 82-83 0.02092439898085985 1105 84-85 -5.410205588218545 1105 86-87 -5.883278006462341 1105 88-89 -1.6254943797296129 1105 90-91 -0.3819473599563139 1105 92-93 -0.020340534260022025 1105 94-95 0.050626627238216315 1105 96-97 0.15312397697759295 1105 98-99 0.20726964520158475 1105 100 0.27813244401747284 1106 1 0.15313037643812066 1106 2 0.21288803676504742 1106 3 0.1951541417769853 1106 4 0.168672431763639 1106 5 0.14637362881240534 1106 6 0.21201767627568557 1106 7 -11.87377978627904 1106 8 -6.462303881599823 1106 9 -2.3836966100008468 1106 10-11 -0.3540958703986803 1106 12-13 0.19542600680565414 1106 14-15 0.37899766965762893 1106 16-17 0.37112705754604747 1106 18-19 0.42728763004319603 1106 20-21 0.4100596099313165 1106 22-23 0.3694338698135766 1106 24-25 0.439655454511211 1106 26-27 0.4002178511501455 1106 28-29 0.4121770842025825 1106 30-31 0.3944840834533636 1106 32-33 0.4606260005377436 1106 34-35 0.4759078713887206 1106 36-37 0.49071590601371895 1106 38-39 0.435715671289465 1106 40-41 0.40160863875962605 1106 42-43 0.3889745050174227 1106 44-45 0.2895344716943171 1106 46-47 0.3988324247624959 1106 48-49 0.35273903408320706 1106 50-51 0.4182945717188531 1106 52-53 0.4082235115491528 1106 54-55 0.39114334128986883 1106 56-57 0.38283132137406284 1106 58-59 0.3511684394544261 1106 60-61 0.33712119394514417 1106 62-63 0.2798450719412031 1106 64-65 0.12339933788742741 1106 66-67 -10.00615312856409 1106 68-69 -8.055485373618929 1106 70-71 -6.584053405202699 1106 72-73 -1.66018079811521 1106 74-75 -0.497820938587509 1106 76-77 -0.2097982698815386 1106 78-79 2.8250611116048248 1106 80-81 0.7913936615783399 1106 82-83 0.1653355255072526 1106 84-85 -5.364447052944108 1106 86-87 -5.763783654999649 1106 88-89 -1.4793067876009722 1106 90-91 -0.24929578448670142 1106 92-93 0.11701218192948204 1106 94-95 0.19494006924895757 1106 96-97 0.308309537199996 1106 98-99 0.33785945540270035 1106 100 0.41456095635031076 1107 1 0.061488426433317755 1107 2 0.08568212206439085 1107 3 0.08545714282007211 1107 4 0.04768341867121961 1107 5 0.09401781973382128 1107 6 0.10696577008872055 1107 7 -11.961896342718802 1107 8 -6.580364967166755 1107 9 -2.503978601078451 1107 10-11 -0.5208596875577527 1107 12-13 0.11050376439559528 1107 14-15 0.22848895610369624 1107 16-17 0.30460963797663965 1107 18-19 0.30282090668676886 1107 20-21 0.24820164593072747 1107 22-23 0.26194405413399835 1107 24-25 0.35603952492058966 1107 26-27 0.19936015550972286 1107 28-29 0.24143793604542907 1107 30-31 0.2758233216914334 1107 32-33 0.330070050735614 1107 34-35 0.250818492643738 1107 36-37 0.3559003355742263 1107 38-39 0.3987357969241643 1107 40-41 0.3819148690237597 1107 42-43 0.3379503394170982 1107 44-45 0.26228472934821667 1107 46-47 0.33198209213380636 1107 48-49 0.28697050101879995 1107 50-51 0.3431508219818369 1107 52-53 0.2883443234928862 1107 54-55 0.20334194484299672 1107 56-57 0.19993991227342178 1107 58-59 0.22311496371257533 1107 60-61 0.20024867532638524 1107 62-63 0.14911010538648384 1107 64-65 -0.01278870405392496 1107 66-67 -10.056061603603428 1107 68-69 -8.130341724316985 1107 70-71 -6.6785614713708625 1107 72-73 -1.7981520851536068 1107 74-75 -0.5553623241717425 1107 76-77 -0.28458955675620246 1107 78-79 2.7775941517938847 1107 80-81 0.7716558493127152 1107 82-83 0.11454232845099455 1107 84-85 -5.365400850347957 1107 86-87 -5.763858879114956 1107 88-89 -1.4938780125961841 1107 90-91 -0.32319195256296496 1107 92-93 0.09489196514528686 1107 94-95 0.18235208360262334 1107 96-97 0.23254709575612154 1107 98-99 0.2931636247041851 1107 100 0.40933858966119274 1108 1 0.08162659379518189 1108 2 0.09308242612260997 1108 3 0.0452449466702447 1108 4 -0.006027797836608784 1108 5 0.005662118053685106 1108 6 0.10042601488565595 1108 7 -11.972181986041573 1108 8 -6.574772646747174 1108 9 -2.564775038026646 1108 10-11 -0.5155907841728862 1108 12-13 0.05179525067347868 1108 14-15 0.2304218432930938 1108 16-17 0.13696130246688654 1108 18-19 0.16575209031886118 1108 20-21 0.23562419228084508 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-2.015836617712452 2216 28-29 -2.2246423514159446 2216 30-31 -2.1488209629872586 2216 32-33 -2.9643904075273753 2216 34-35 -2.071699135778182 2216 36-37 -1.742695812033297 2216 38-39 -2.001507887934288 2216 40-41 -2.0403901419434476 2216 42-43 -1.7437543088112974 2216 44-45 -1.837076146631567 2216 46-47 -2.023824123193492 2216 48-49 -2.112335412752536 2216 50-51 -2.5723060127925095 2216 52-53 -2.32644645023084 2216 54-55 -1.9735789912954402 2216 56-57 -1.9863280687570821 2216 58-59 -2.328397737852079 2216 60-61 -2.421504200351073 2216 62-63 -2.2765813171026252 2216 64-65 -1.9173204791407201 2216 66-67 1.2805187130759244 2216 68-69 0.47420861236076917 2216 70-71 0.134116592736774 2216 72-73 -1.6036387477895886 2216 74-75 -2.409791323068454 2216 76-77 -2.0821094876354564 2216 78-79 0.5502218164581478 2216 80-81 -0.9265141945876607 2216 82-83 -1.7857321944256555 2216 84-85 -0.03299540779759269 2216 86-87 -0.17762838254860114 2216 88-89 -1.325594674892102 2216 90-91 -1.6071242741378633 2216 92-93 -1.6140557797396866 2216 94-95 -1.666867376193288 2216 96-97 -1.7345925012857428 2216 98-99 -1.7569881539713457 2216 100 -2.7972017253344674 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 182577.0 3 33029.0 4 19340.0 5 16345.0 6 14753.0 7 15802.0 8 17864.0 9 20636.0 10 22960.0 11 26165.0 12 28707.0 13 32400.0 14 36202.0 15 38917.0 16 40817.0 17 41811.0 18 39734.0 19 37519.0 20 36047.0 21 36266.0 22 38190.0 23 43472.0 24 52336.0 25 66320.0 26 82953.0 27 100596.0 28 122456.0 29 151727.0 30 183638.0 31 222176.0 32 290174.0 33 398582.0 34 584515.0 35 776349.0 36 868694.0 37 1411766.0 38 1590452.0 39 521479.0 40 1117.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.66917097496493 21.412539543785 15.263227743329994 22.655061737920086 2 14.757951804437782 20.53646335992051 41.45385017601002 23.251734659631683 3 18.058500515841413 25.597305059127095 30.791620688090966 25.552573736940527 4 12.419348343836221 17.378706555811018 39.50178514335727 30.70015995699549 5 12.76659847376797 38.1397177767328 36.01263058463029 13.081053164868935 6 30.051725990763888 38.58484064353411 18.130222965388974 13.233210400313029 7 26.265285072904092 33.0566188819005 23.049805441162388 17.628290604033023 8 23.86764008658461 37.41913620848228 20.98479747984295 17.728426225090153 9 24.05648448862161 17.90238866943291 22.522163140459362 35.51896370148612 10-11 23.17960121447448 27.832493196082954 29.377348350485992 19.610557238956574 12-13 23.467149260941607 25.266119604122945 29.53552524638615 21.731205888549297 14-15 20.90331057457092 27.746172989962382 26.669972151211844 24.68054428425485 16-17 20.439616738348672 31.097603980951295 27.51471264503045 20.94806663566959 18-19 20.340258315369667 30.120560837552212 29.435645114672944 20.103535732405177 20-21 21.46434946378257 29.230554272268023 28.95067158624325 20.35442467770616 22-23 21.17645703258961 29.037296771664124 29.06846762218867 20.717778573557595 24-25 20.953911656478326 29.19242152981431 28.996154342032504 20.857512471674855 26-27 20.775632323263196 29.211832614037842 29.03177526203254 20.980759800666426 28-29 20.7563042434926 29.233822966317412 29.202912885483034 20.806959904706957 30-31 21.089183436563015 29.525743421707745 28.75354325828517 20.63152988344407 32-33 20.747367882281395 29.328236595745093 29.099810039731317 20.8245854822422 34-35 20.96954970473187 29.07323442830392 29.099996494793924 20.857219372170288 36-37 21.10047178358647 29.301547311410886 28.76504478778471 20.83293611721793 38-39 20.974341533855977 29.41963019235814 28.843454502254183 20.76257377153169 40-41 20.931432315742523 29.18729890935377 29.011238183200305 20.870030591703397 42-43 20.8768906577438 29.159350006464617 28.961227098216806 21.002532237574776 44-45 21.00303679669011 29.22705167527161 28.846609140808976 20.9233023872293 46-47 20.98139890992063 29.31369270071333 28.783953546548208 20.92095484281783 48-49 20.861779682997845 29.218964822030667 28.851565043733146 21.06769045123834 50-51 20.90591140634701 29.186176303603567 28.8061939703745 21.10171831967492 52-53 20.959367121040525 29.1821725347148 28.74527622504317 21.113184119201502 54-55 21.01167492151466 29.384481370920913 28.650477898208482 20.953365809355947 56-57 20.957246029951243 29.182827676199196 28.668058701237932 21.19186759261163 58-59 20.91322842378486 28.925322278511246 28.92820286509813 21.233246432605764 60-61 20.997878231585137 29.20766107158674 28.692098090990616 21.102362605837506 62-63 20.857507230261035 29.249364606073353 28.724353867341378 21.168774296324234 64-65 20.95411584192719 29.044765270406042 28.751264964667712 21.24985392299906 66-67 20.829806597102753 29.057731880263248 28.723668172445336 21.38879335018866 68-69 21.290829906957313 29.107991855628313 28.584693707235004 21.016484530179373 70-71 21.017868840866853 28.97806793635708 28.6880508623311 21.316012360444976 72-73 20.874210948049758 29.069532268494374 28.863098709626517 21.19315807382935 74-75 20.87672738543949 29.106482937813183 28.728765243446674 21.288024433300652 76-77 20.935451872793507 29.01931148114446 28.59331436454854 21.45192228151349 78-79 21.052659944792165 29.041865011881622 28.732475028120092 21.17300001520612 80-81 20.900516041309988 28.9275631800371 28.80345005970651 21.3684707189464 82-83 20.838091800311588 28.994697019454573 28.90964046457799 21.257570715655845 84-85 20.889339200555355 28.87926336249336 28.731064642085112 21.50033279486617 86-87 20.99809530648027 28.958649283803823 28.718826616125508 21.3244287935904 88-89 20.846402484097336 28.79479713522477 28.817350839147576 21.54144954153032 90-91 20.79568407505056 28.759529385737064 28.817949237012364 21.626837302200013 92-93 20.889174220657452 28.916647012372408 28.699195418185262 21.494983348784874 94-95 20.807765386404448 28.714142155985755 28.83617450832582 21.641917949283982 96-97 20.830473896342223 28.761824363252202 28.773365208626707 21.634336531778867 98-99 20.83527908579513 28.69256714877662 28.808857445259566 21.663296320168683 100 20.909783498493468 28.587397808191064 28.69207463032889 21.81074406298658 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1835.0 1 1488.5 2 1268.0 3 1404.0 4 1327.5 5 1168.5 6 1063.5 7 1024.5 8 1078.5 9 1141.5 10 1085.5 11 1038.5 12 1090.5 13 1192.0 14 1350.0 15 1626.5 16 2020.0 17 2677.5 18 3859.5 19 5591.5 20 8364.5 21 12629.0 22 19155.5 23 29291.5 24 42185.0 25 57695.5 26 77489.0 27 100960.5 28 128098.0 29 157160.0 30 184865.0 31 209781.0 32 232538.0 33 254492.5 34 273779.5 35 288695.0 36 299693.5 37 310029.5 38 318473.0 39 321157.0 40 319440.5 41 313267.5 42 303873.0 43 294329.0 44 283616.5 45 273184.0 46 263801.0 47 252980.5 48 242927.5 49 235083.5 50 225380.0 51 212348.0 52 198748.5 53 186148.5 54 175526.5 55 164980.5 56 149883.5 57 133256.5 58 118386.5 59 103556.0 60 87530.5 61 71651.0 62 58486.5 63 47788.0 64 38820.0 65 31057.5 66 24446.5 67 18766.0 68 14272.5 69 11001.5 70 8333.0 71 6114.5 72 4513.5 73 3286.5 74 2424.5 75 1735.0 76 1141.0 77 800.5 78 543.0 79 367.5 80 244.0 81 165.5 82 115.5 83 80.5 84 69.0 85 59.0 86 48.0 87 40.0 88 35.0 89 27.5 90 22.5 91 18.5 92 15.0 93 19.0 94 21.0 95 17.0 96 15.5 97 16.5 98 18.0 99 26.0 100 42.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.018059686232054478 2 0.0022074297476386263 3 0.0012250022225906662 4 0.002025498724481597 5 0.0016252504735361315 6 0.0034566894399835632 7 11.723901964406288 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.3651033010413852E-4 18-19 0.0 20-21 0.0 22-23 6.064367438567655E-6 24-25 0.0 26-27 1.212873487713531E-4 28-29 0.0 30-31 0.0 32-33 1.212873487713531E-5 34-35 4.851493950854124E-5 36-37 1.212873487713531E-5 38-39 2.425746975427062E-5 40-41 6.064367438567655E-6 42-43 1.212873487713531E-5 44-45 1.3341608364848842E-4 46-47 8.550758088380394E-4 48-49 6.003723764181978E-4 50-51 3.1534710680551804E-4 52-53 2.486390649812739E-4 54-55 3.759907811911946E-4 56-57 0.0014129976131862635 58-59 4.184413532611682E-4 60-61 0.0013280964690463165 62-63 0.0023590389336028174 64-65 0.0010612643017493396 66-67 5.862296651147142 68-69 5.497282375019755 70-71 0.0023772320359185207 72-73 0.0012068091202749633 74-75 0.002832059593811095 76-77 0.0055913467783593775 78-79 5.481793980581653 80-81 0.0047059491323285 82-83 0.004469438802224362 84-85 5.862363359188967 86-87 0.0030261193518452594 88-89 0.0014251263480633987 90-91 0.0030806986587923685 92-93 0.003511268746930672 94-95 0.004220799737243088 96-97 0.0030685699239152335 98-99 0.005203227262291048 100 0.0055792180434822425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8244883.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.12546679027324 #Duplication Level Percentage of deduplicated Percentage of total 1 91.56879086774187 80.6954243864066 2 6.291494841886303 11.088818394996535 3 1.2337196937958919 3.261663717123478 4 0.4055192510600328 1.4294629316842955 5 0.18734456585546314 0.8254913658316888 6 0.0964395705931574 0.5099269305345302 7 0.060876922834326226 0.3755365069071309 8 0.03972096881349787 0.2800343134441108 9 0.023641534522766464 0.18750791389114388 >10 0.09075219290444408 1.2258991170905025 >50 0.0013769499180167962 0.08193148337890838 >100 3.226400742440906E-4 0.038302938711078635 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.212873487713531E-5 0.0 0.0 0.0 6 0.0 1.212873487713531E-5 0.0 0.0 0.0 7 0.0 1.212873487713531E-5 0.0 0.0 0.0 8 0.0 1.212873487713531E-5 0.0 3.638620463140593E-5 0.0 9 0.0 1.212873487713531E-5 0.0 4.851493950854124E-5 0.0 10-11 0.0 1.212873487713531E-5 0.0 4.851493950854124E-5 0.0 12-13 0.0 2.425746975427062E-5 0.0 4.851493950854124E-5 0.0 14-15 0.0 6.670804182424421E-5 0.0 6.064367438567655E-5 0.0 16-17 0.0 9.702987901708248E-5 0.0 7.277240926281186E-5 0.0 18-19 0.0 9.702987901708248E-5 0.0 8.490114413994716E-5 0.0 20-21 0.0 9.702987901708248E-5 0.0 9.096551157851483E-5 0.0 22-23 0.0 1.0309424645565013E-4 0.0 1.8193102315702966E-4 0.0 24-25 0.0 1.0915861389421778E-4 0.0 3.2747584168265335E-4 0.0 26-27 0.0 1.212873487713531E-4 0.0 8.42947073960904E-4 0.0 28-29 0.0 1.334160836484884E-4 0.0 0.0024621331800584677 0.0 30-31 0.0 1.334160836484884E-4 0.0 0.008168702939750631 0.0 32-33 0.0 1.334160836484884E-4 0.0 0.021091869951338306 0.0 34-35 0.0 1.5160918596419137E-4 0.0 0.04190477900050249 0.0 36-37 0.0 1.6980228827989433E-4 0.0 0.0750889976243447 0.0 38-39 0.0 1.9405975803416496E-4 0.0 0.11772756508491387 0.0 40-41 0.0 2.425746975427062E-4 0.0 0.16311935536259278 0.0 42-43 0.0 2.425746975427062E-4 0.0 0.20922067663058408 0.0 44-45 0.0 2.425746975427062E-4 0.0 0.26791162470104185 0.0 46-47 0.0 2.425746975427062E-4 0.0 0.3385796984626708 1.212873487713531E-5 48-49 0.0 2.486390649812738E-4 0.0 0.4134746363289813 1.212873487713531E-5 50-51 0.0 3.214114742440857E-4 0.0 0.49023133499893207 1.212873487713531E-5 52-53 0.0 3.3960457655978865E-4 0.0 0.5653324613581538 1.212873487713531E-5 54-55 0.0 3.3960457655978865E-4 0.0 0.6484991964106708 1.212873487713531E-5 56-57 0.0 3.3960457655978865E-4 0.0 0.7408595125000561 1.212873487713531E-5 58-59 0.0 3.5173331143692395E-4 0.0 0.8407517729481425 1.212873487713531E-5 60-61 0.0 3.5173331143692395E-4 0.0 0.9416082678189612 1.212873487713531E-5 62-63 0.0 3.5173331143692395E-4 0.0 1.0430408775964437 1.212873487713531E-5 64-65 0.0 3.5173331143692395E-4 0.0 1.1546373672009658 1.212873487713531E-5 66-67 0.0 3.5173331143692395E-4 0.0 1.2760641964234058 1.212873487713531E-5 68-69 0.0 3.5173331143692395E-4 0.0 1.4002988277699029 1.212873487713531E-5 70-71 0.0 3.5173331143692395E-4 0.0 1.5479540461641481 1.212873487713531E-5 72-73 0.0 3.881195160683299E-4 0.0 1.7041600226467737 1.212873487713531E-5 74-75 0.0 4.002482509454652E-4 0.0 1.848740606749665 1.212873487713531E-5 76-77 0.0 4.002482509454652E-4 0.0 1.999585682416597 1.212873487713531E-5 78-79 0.0 4.0631261838403287E-4 0.0 2.177714347189645 1.212873487713531E-5 80-81 0.0 4.305700881383035E-4 0.0 2.3902401040742483 2.425746975427062E-5 82-83 0.0 4.426988230154388E-4 0.0 2.6053371527528046 3.638620463140593E-5 84-85 0.0 4.6089192533114174E-4 0.0 2.827329387209012 3.638620463140593E-5 86-87 0.0 4.669562927697094E-4 0.0 3.092372566111611 3.638620463140593E-5 88 0.0 4.7302066020827704E-4 0.0 3.3133399224707007 3.638620463140593E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7160 0.0 41.78961 1 GTATCAA 11865 0.0 35.19317 1 ATCAACG 12050 0.0 33.11649 3 TCAACGC 12000 0.0 32.998116 4 CAACGCA 13685 0.0 29.196566 5 TATCAAC 14330 0.0 28.204908 2 AACGCAG 14715 0.0 27.08291 6 GTACATG 19135 0.0 23.589348 1 ACGCAGA 16615 0.0 23.553911 7 CGCAGAG 16850 0.0 22.97175 8 TACATGG 19240 0.0 22.391584 2 ACATGGG 20225 0.0 20.54122 3 GCAGAGT 19870 0.0 18.410725 9 CATGGGG 14640 0.0 17.495237 4 GTGGTAT 4835 0.0 17.166708 1 AGAGTAC 16715 0.0 16.570002 10-11 GAGTACA 14945 0.0 16.044212 1 GTATTAG 3730 0.0 15.659018 1 CAGAGTA 19160 0.0 15.175335 10-11 TATACTG 4770 0.0 14.9272 5 >>END_MODULE