FastQCFastQC Report
Wed 25 May 2016
SRR2049515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049515_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8244883
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192130.2330293831944007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189920.23034893278655377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107560.1304566723384674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140900.055.4706881
GTATCAA254050.038.975041
ATCAACG307900.031.7263723
TCAACGC310150.031.4808464
TATCAAC324550.030.1277482
CAACGCA324900.030.0886335
GTGGTAT63600.029.686141
AACGCAG340400.028.731846
TGGTATC63150.028.5383032
ACGCAGA382950.025.2688037
CGCAGAG382100.025.1405188
GCAGAGT419700.022.4515239
GTACATG197600.021.4209751
GAGTACT299050.021.13726812-13
CAGAGTA411950.020.164410-11
AGAGTAC386900.020.02444810-11
TACATGG204550.020.0134742
GTACTTT328450.019.10215414-15
AGTACTT317750.018.82839212-13
ACATGGG208300.018.772173
Produced by FastQC (version 0.11.5)