##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049515_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8244883
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.750044118273117	31.0	31.0	34.0	30.0	34.0
2	31.795442700642326	31.0	31.0	34.0	30.0	34.0
3	31.870937161873613	33.0	31.0	34.0	30.0	34.0
4	35.57671697706323	37.0	35.0	37.0	33.0	37.0
5	35.72932217473553	37.0	35.0	37.0	33.0	37.0
6	35.82797633392736	37.0	35.0	37.0	35.0	37.0
7	35.796138526162224	37.0	35.0	37.0	33.0	37.0
8	35.795262103780004	37.0	35.0	37.0	33.0	37.0
9	37.519182382575956	39.0	37.0	39.0	35.0	39.0
10-11	37.491004905709396	39.0	37.0	39.0	34.5	39.0
12-13	37.48788139261649	39.0	37.0	39.0	34.5	39.0
14-15	38.7776338972912	40.0	38.0	41.0	35.0	41.0
16-17	38.793736308932466	40.0	38.0	41.0	35.0	41.0
18-19	38.859296244713235	40.0	38.0	41.0	35.0	41.0
20-21	38.89192824203812	40.0	38.0	41.0	35.0	41.0
22-23	38.87646871398903	40.0	38.0	41.0	35.0	41.0
24-25	38.854535837561315	40.0	38.0	41.0	35.0	41.0
26-27	38.77219888990541	40.0	38.0	41.0	35.0	41.0
28-29	38.690033199985976	40.0	38.0	41.0	34.5	41.0
30-31	38.58717231038936	40.0	38.0	41.0	34.0	41.0
32-33	38.46324459667893	40.0	38.0	41.0	34.0	41.0
34-35	38.374807441172905	40.0	38.0	41.0	34.0	41.0
36-37	38.24551306549772	40.0	38.0	41.0	34.0	41.0
38-39	38.160627628069435	40.0	38.0	41.0	34.0	41.0
40-41	38.048440105214354	40.0	38.0	41.0	33.0	41.0
42-43	37.92072070640663	40.0	38.0	41.0	33.0	41.0
44-45	37.76134991848883	40.0	37.0	41.0	33.0	41.0
46-47	37.55197265989099	40.0	37.0	41.0	33.0	41.0
48-49	37.349478579623266	40.0	37.0	41.0	32.0	41.0
50-51	37.1587978264822	40.0	37.0	41.0	32.0	41.0
52-53	37.072352633748714	40.0	37.0	41.0	31.5	41.0
54-55	36.963012998486455	40.0	36.5	41.0	31.0	41.0
56-57	36.80761697891893	40.0	36.0	41.0	31.0	41.0
58-59	36.65716317623913	39.0	36.0	41.0	31.0	41.0
60-61	36.49288152421326	39.0	35.0	41.0	31.0	41.0
62-63	36.31755107986372	39.0	35.0	41.0	31.0	41.0
64-65	36.07077280538729	39.0	35.0	41.0	30.0	41.0
66-67	35.83689313723433	38.0	35.0	40.0	30.0	41.0
68-69	35.54631430185243	37.5	35.0	40.0	29.5	41.0
70-71	35.26688213768467	37.0	35.0	40.0	29.0	41.0
72-73	34.96296806152374	37.0	34.0	39.0	29.0	41.0
74-75	34.61915159984684	36.0	34.0	39.0	29.0	41.0
76-77	34.31868420691961	36.0	34.0	39.0	29.0	40.5
78-79	33.97683132677565	35.0	34.0	37.5	29.0	39.5
80-81	33.646153620372786	35.0	34.0	37.0	29.0	39.0
82-83	33.33405179915834	35.0	34.0	37.0	29.0	39.0
84-85	33.03967394079455	35.0	34.0	36.0	28.0	38.0
86-87	32.751158506433626	35.0	34.0	36.0	27.5	37.0
88-89	32.520294587564194	35.0	33.0	36.0	27.0	37.0
90-91	32.35105774090427	35.0	33.0	35.0	27.0	36.5
92-93	32.18281181188381	35.0	33.0	35.0	27.0	36.0
94-95	31.996319899263582	35.0	33.0	35.0	27.0	36.0
96-97	31.825974364948536	35.0	33.0	35.0	26.0	36.0
98-99	31.645751007018532	35.0	33.0	35.0	26.0	36.0
100	31.534948403755397	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.36466722555649156
1101	2	0.4473247534890703
1101	3	0.48699891155789743
1101	4	0.4075952921655599
1101	5	0.30848031053578495
1101	6	0.30629180174024384
1101	7	0.29711413886970917
1101	8	0.32492069827605974
1101	9	0.3345379459051401
1101	10-11	0.3619681749403938
1101	12-13	0.33461031376454997
1101	14-15	0.5007186751178025
1101	16-17	0.5025499902509054
1101	18-19	0.4773756691990272
1101	20-21	0.44784717810138375
1101	22-23	0.4748201568163708
1101	24-25	0.5169700642474666
1101	26-27	0.5297068993565404
1101	28-29	0.48412241341067386
1101	30-31	0.5018699965584545
1101	32-33	0.5715801872406558
1101	34-35	0.5767842222356876
1101	36-37	0.5677882159200109
1101	38-39	0.5395756504131768
1101	40-41	0.5841485579738546
1101	42-43	0.4816137587311502
1101	44-45	0.5028880921560273
1101	46-47	0.5352556319989361
1101	48-49	0.4942553628655233
1101	50-51	0.5566836563110371
1101	52-53	0.5374540984636909
1101	54-55	0.4680708201281547
1101	56-57	0.42244624096481687
1101	58-59	0.42749620954661793
1101	60-61	0.49327842794934185
1101	62-63	0.462335065477653
1101	64-65	0.4927184485710825
1101	66-67	0.46438024738895933
1101	68-69	0.47231945082361904
1101	70-71	0.4534785344452672
1101	72-73	0.4340095511378834
1101	74-75	0.44457866578012784
1101	76-77	0.41814893378509765
1101	78-79	0.4178452955864387
1101	80-81	0.44477215185728625
1101	82-83	0.44664417102229237
1101	84-85	0.42055137633504813
1101	86-87	0.4769028473103063
1101	88-89	0.46411683842636364
1101	90-91	0.4301339802568265
1101	92-93	0.4363609641810342
1101	94-95	0.37776188222643015
1101	96-97	0.40620543823508726
1101	98-99	0.4571678539288797
1101	100	0.5345969791205114
1102	1	0.25239881223411587
1102	2	0.3321905972608761
1102	3	0.4033291965964949
1102	4	0.37166989803998973
1102	5	0.26505640445866874
1102	6	0.29397309303397634
1102	7	0.3077212358029513
1102	8	0.31626116795789017
1102	9	0.43859828330253947
1102	10-11	0.3590702801427881
1102	12-13	0.33185706807820026
1102	14-15	0.396421433389591
1102	16-17	0.41788521104867726
1102	18-19	0.40167780010642673
1102	20-21	0.4452915774489341
1102	22-23	0.4232880878672489
1102	24-25	0.3974224708815868
1102	26-27	0.4040331287019825
1102	28-29	0.421092162629364
1102	30-31	0.49844897816569045
1102	32-33	0.5129505761395592
1102	34-35	0.538149437796811
1102	36-37	0.5516900843281647
1102	38-39	0.5397622937618678
1102	40-41	0.5410167882650683
1102	42-43	0.5141995393660821
1102	44-45	0.5154913069186193
1102	46-47	0.5599118706184072
1102	48-49	0.5265249706172597
1102	50-51	0.6037948642210793
1102	52-53	0.6034289100400443
1102	54-55	0.5657465094380925
1102	56-57	0.5680274612326599
1102	58-59	0.5472064682293336
1102	60-61	0.58480952126434
1102	62-63	0.5578765723452435
1102	64-65	0.5452546466267378
1102	66-67	0.6080370224695812
1102	68-69	0.5811764782480822
1102	70-71	0.5856025539174965
1102	72-73	0.6048412799154548
1102	74-75	0.603638725555264
1102	76-77	0.5752955225367984
1102	78-79	0.5402637749223729
1102	80-81	0.5421460757441992
1102	82-83	0.564965251936826
1102	84-85	0.5678467953888244
1102	86-87	0.5560656761731408
1102	88-89	0.5509435554168505
1102	90-91	0.5611615328387245
1102	92-93	0.5589398658385605
1102	94-95	0.6116218836836893
1102	96-97	0.5786625060334778
1102	98-99	0.6217585929593525
1102	100	0.6688357577972219
1103	1	0.3512364975189719
1103	2	0.38141709409184443
1103	3	0.44752432410066945
1103	4	0.39162376151864464
1103	5	0.2926510874082382
1103	6	0.3034980245257799
1103	7	0.3169317956459068
1103	8	0.31183031466388655
1103	9	0.36683769286625534
1103	10-11	0.35383360958343246
1103	12-13	0.3249112250476074
1103	14-15	0.4703900710120621
1103	16-17	0.4255675937607535
1103	18-19	0.4301992175850202
1103	20-21	0.4464889197841089
1103	22-23	0.41542686077873014
1103	24-25	0.4314562132179489
1103	26-27	0.47007228749639296
1103	28-29	0.45745212280012737
1103	30-31	0.4874049863208185
1103	32-33	0.4691169429397988
1103	34-35	0.49935609215114596
1103	36-37	0.5132751759641891
1103	38-39	0.548228222989863
1103	40-41	0.5634932856751362
1103	42-43	0.5519304577515598
1103	44-45	0.616490098738737
1103	46-47	0.6228074287455598
1103	48-49	0.6728449526418174
1103	50-51	0.7079917422789634
1103	52-53	0.6898840089992788
1103	54-55	0.6479905836152398
1103	56-57	0.693552221971018
1103	58-59	0.652468983447406
1103	60-61	0.6401047642256401
1103	62-63	0.6069450726038568
1103	64-65	0.6314325525210691
1103	66-67	0.6805895481810538
1103	68-69	0.6737107637855644
1103	70-71	0.6845708192577575
1103	72-73	0.6729729791339238
1103	74-75	0.6330933304546278
1103	76-77	0.6097876683747998
1103	78-79	0.5797952651995146
1103	80-81	0.5638155314940789
1103	82-83	0.6110772853886175
1103	84-85	0.6439407177244689
1103	86-87	0.6401623424769056
1103	88-89	0.6477476201429937
1103	90-91	0.6326625184765717
1103	92-93	0.6425940788653932
1103	94-95	0.6597677693364616
1103	96-97	0.6582628801026438
1103	98-99	0.7309826376914543
1103	100	0.7453252920341136
1104	1	0.285960375381098
1104	2	0.2901716793129623
1104	3	0.3856215479813301
1104	4	0.3224608823031758
1104	5	0.22249742906256387
1104	6	0.2852420016634909
1104	7	0.23614238511925123
1104	8	0.2619464099979041
1104	9	0.2738706528480108
1104	10-11	0.289879236397951
1104	12-13	0.3150384339560972
1104	14-15	0.4011736423059915
1104	16-17	0.4029208997116953
1104	18-19	0.4157356668242045
1104	20-21	0.38471576791405937
1104	22-23	0.4090886367454587
1104	24-25	0.40250421618915766
1104	26-27	0.4294349989782589
1104	28-29	0.4364243052455876
1104	30-31	0.45610719469593164
1104	32-33	0.4600092496715149
1104	34-35	0.4592105626699592
1104	36-37	0.4309722734193002
1104	38-39	0.5088275179508841
1104	40-41	0.5081026246922278
1104	42-43	0.4753285431805381
1104	44-45	0.5112878493722519
1104	46-47	0.5130300645672179
1104	48-49	0.4584010643097045
1104	50-51	0.5338886258751785
1104	52-53	0.525563365102883
1104	54-55	0.49091282419552584
1104	56-57	0.47622944956154356
1104	58-59	0.5112006803476277
1104	60-61	0.5114788014199689
1104	62-63	0.5030103829585144
1104	64-65	0.5184527394411376
1104	66-67	0.510388814520077
1104	68-69	0.4709829768522056
1104	70-71	0.4959104748706551
1104	72-73	0.5512522936735778
1104	74-75	0.5833880413764589
1104	76-77	0.4599669348987234
1104	78-79	0.4641074491656383
1104	80-81	0.4732295133905069
1104	82-83	0.4589521869205342
1104	84-85	0.5245967231346285
1104	86-87	0.5598976824122133
1104	88-89	0.5040314198403237
1104	90-91	0.4863330288223153
1104	92-93	0.4650537159488408
1104	94-95	0.4810014746224667
1104	96-97	0.48090840982373706
1104	98-99	0.5232815709655085
1104	100	0.553325664619063
1105	1	0.1906526372645203
1105	2	0.211484391624726
1105	3	0.29607389012299024
1105	4	0.251820465351436
1105	5	0.18780151037007897
1105	6	0.22226260186558733
1105	7	0.23452765969526013
1105	8	0.24180783610905365
1105	9	0.2539265214909321
1105	10-11	0.23527610036259006
1105	12-13	0.2165855344187051
1105	14-15	0.29895087930781017
1105	16-17	0.2838393988687713
1105	18-19	0.28543078372409525
1105	20-21	0.26654164695471394
1105	22-23	0.2709340562531608
1105	24-25	0.3083260177702343
1105	26-27	0.3353845474119055
1105	28-29	0.3602548763119273
1105	30-31	0.35336333005213305
1105	32-33	0.33528028281588007
1105	34-35	0.3100168693231211
1105	36-37	0.333172747903717
1105	38-39	0.328507257679469
1105	40-41	0.34476725588582724
1105	42-43	0.2803489526411198
1105	44-45	0.30676371255093926
1105	46-47	0.3365459201824521
1105	48-49	0.3574100031549463
1105	50-51	0.4102920783140718
1105	52-53	0.37334342001806675
1105	54-55	0.3683280101330837
1105	56-57	0.38087491877064394
1105	58-59	0.3777721878579996
1105	60-61	0.37152239919859653
1105	62-63	0.35300448830902553
1105	64-65	0.3618136453433394
1105	66-67	0.3970774957720238
1105	68-69	0.3683515094767813
1105	70-71	0.3695231081884742
1105	72-73	0.37853789186234366
1105	74-75	0.3974306587034988
1105	76-77	0.3286097311723992
1105	78-79	0.32760193228268264
1105	80-81	0.31343527692256146
1105	82-83	0.3214212018847107
1105	84-85	0.32897292430855885
1105	86-87	0.37151128693219704
1105	88-89	0.3438561466826613
1105	90-91	0.3413252092444523
1105	92-93	0.37440320658349435
1105	94-95	0.39536129643859397
1105	96-97	0.4269245761967575
1105	98-99	0.4654574027959626
1105	100	0.47460915940644455
1106	1	0.19127899383036961
1106	2	0.19756407849641633
1106	3	0.3115388793532219
1106	4	0.26497093317559717
1106	5	0.18548813946254228
1106	6	0.20599164047899876
1106	7	0.20695171401408174
1106	8	0.21796595923657236
1106	9	0.2327279689163504
1106	10-11	0.2380653162629045
1106	12-13	0.20141690641870724
1106	14-15	0.307624817280967
1106	16-17	0.31994108992364545
1106	18-19	0.2731655675915903
1106	20-21	0.27059569816653095
1106	22-23	0.27781496623901347
1106	24-25	0.2930775559582983
1106	26-27	0.3086683237401999
1106	28-29	0.3412907027342271
1106	30-31	0.33056458682556666
1106	32-33	0.3386105536060242
1106	34-35	0.33500886122418194
1106	36-37	0.3501551308410811
1106	38-39	0.3560074861762743
1106	40-41	0.3452848214306954
1106	42-43	0.3643864794582896
1106	44-45	0.35960898319633827
1106	46-47	0.3673626104909644
1106	48-49	0.35157930657548064
1106	50-51	0.36935330495150964
1106	52-53	0.3917540779679811
1106	54-55	0.3205533939691989
1106	56-57	0.39709316695123675
1106	58-59	0.38560596828884286
1106	60-61	0.4130621792102147
1106	62-63	0.4102907821438251
1106	64-65	0.40977096307035055
1106	66-67	0.4399489628494564
1106	68-69	0.42088931238663463
1106	70-71	0.4671824267229141
1106	72-73	0.4257944181656157
1106	74-75	0.4427148919714625
1106	76-77	0.4550970044385778
1106	78-79	0.43298478113119643
1106	80-81	0.3756589172141105
1106	82-83	0.39271250231758614
1106	84-85	0.42299070527528926
1106	86-87	0.46835617534842555
1106	88-89	0.4541597436497966
1106	90-91	0.3814640385835588
1106	92-93	0.39410396587117447
1106	94-95	0.4026017342183863
1106	96-97	0.4283517786091231
1106	98-99	0.4617773445002058
1106	100	0.4832030358648467
1107	1	0.21857213054967417
1107	2	0.18711418322275009
1107	3	0.25854208431803727
1107	4	0.23746451791473078
1107	5	0.1699343733640717
1107	6	0.1581561102394673
1107	7	0.15672334159977197
1107	8	0.20819171753623067
1107	9	0.18673685865793033
1107	10-11	0.21232396559971534
1107	12-13	0.17972697215800792
1107	14-15	0.22145457278428893
1107	16-17	0.2320785466210893
1107	18-19	0.2345639354586453
1107	20-21	0.22663106397049404
1107	22-23	0.25553752185700773
1107	24-25	0.2824493767577039
1107	26-27	0.29037166713374063
1107	28-29	0.27988984851527476
1107	30-31	0.2894854609362625
1107	32-33	0.30332267732531193
1107	34-35	0.2801624843124628
1107	36-37	0.35315654238591065
1107	38-39	0.35998684917525026
1107	40-41	0.3499253887832765
1107	42-43	0.35289375284340707
1107	44-45	0.33743331107172025
1107	46-47	0.3545022589502338
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2216	100	-1.6058147775879732
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	453.0
3	42.0
4	72.0
5	128.0
6	340.0
7	957.0
8	2152.0
9	3795.0
10	5627.0
11	8296.0
12	11825.0
13	16923.0
14	20872.0
15	27174.0
16	34302.0
17	47879.0
18	47150.0
19	25435.0
20	18924.0
21	18291.0
22	19299.0
23	21535.0
24	25714.0
25	31854.0
26	41291.0
27	53985.0
28	71609.0
29	94581.0
30	124753.0
31	162353.0
32	210026.0
33	272841.0
34	370856.0
35	528733.0
36	856911.0
37	1580408.0
38	2400819.0
39	1082340.0
40	4338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.82585635552216	21.8626146740559	15.583936299755804	22.727592670666137
2	15.023333928855806	21.037880444558134	40.609235255755074	23.329550370830994
3	17.680776714666955	25.948621938873345	31.248443731328567	25.122157615131137
4	12.531441198735092	17.967074079677833	38.879196096179164	30.62228862540791
5	12.342332923294423	38.148124878559024	36.08675148997919	13.422790708167359
6	29.646556630068122	39.1681055582859	17.55621623011528	13.629121581530695
7	25.581466710928463	33.63985880697155	22.917960145704917	17.86071433639507
8	24.057685233374446	37.31471992992502	20.475402743738147	18.152192092962384
9	24.006762499058507	17.484533736751327	22.489602942722883	36.01910082146728
10-11	23.24116060834338	27.81963673711319	29.01552999599873	19.9236726585447
12-13	23.87056139011822	25.21107792756027	29.585980101715226	21.332380580606284
14-15	20.992909358369797	27.403656438239892	27.333261248205933	24.27017295518438
16-17	19.915685886628108	30.80691987988186	28.213450694206333	21.0639435392837
18-19	19.76340962024567	29.10143782513348	31.186221805694515	19.948930748926333
20-21	20.774327543520023	28.2429599061624	31.042035405475126	19.940677144842446
22-23	20.47893826995483	28.041004341723223	31.209794001928227	20.27026338639372
24-25	20.351556231907715	28.16982363485328	31.102733659167754	20.37588647407125
26-27	20.20861909138068	28.179174889443548	31.14577247487927	20.466433544296507
28-29	20.21086322702097	28.21185608676328	31.281679924505788	20.295600761709963
30-31	20.491284028456988	28.465236054833653	30.89209840210827	20.15138151460109
32-33	20.191050618911145	28.292711976628414	31.203869114940748	20.312368289519693
34-35	20.450472129198193	28.089404058250434	31.09977424785773	20.360349564693642
36-37	20.564736940475687	28.35821927369982	30.683837478348693	20.3932063074758
38-39	20.48402020986835	28.430476211730355	30.734232371763188	20.351271206638106
40-41	20.44989601429153	28.30456781497081	30.77640398293099	20.469132187806668
42-43	20.478792725136305	28.274591646721976	30.640246805200267	20.606368822941455
44-45	20.630286688119163	28.395963896637465	30.410110125274066	20.563639289969306
46-47	20.680627002226714	28.554692649974534	30.073131419815173	20.691548927983575
48-49	20.708941533797386	28.556154162527232	29.84529919951563	20.889605104159756
50-51	20.846632996490065	28.601085060879576	29.603622028353826	20.948659914276526
52-53	20.801599003891262	28.710170902364535	29.51919390487409	20.969036188870117
54-55	20.881951872452284	28.9204103927248	29.367232985598463	20.830404749224456
56-57	20.77489152969181	28.67821775033072	29.42321922579132	21.12367149418615
58-59	20.723630309405618	28.454361880407376	29.691276020867758	21.130731789319253
60-61	20.817960667240516	28.68312988795596	29.47000582057987	21.028903624223656
62-63	20.663913605566023	28.678381488251564	29.560716628725963	21.096988277456454
64-65	20.66641818931815	28.53634187410543	29.623155355873454	21.174084580702964
66-67	20.61713307514491	28.551866654748164	29.556253254291175	21.27474701581575
68-69	20.94792612581646	28.639981913630553	29.394789471239314	21.017302489313675
70-71	20.869592691612482	28.528706835500273	29.380307761796015	21.22139271109123
72-73	20.732362120845135	28.633104920955216	29.465979080600658	21.168553877598992
74-75	20.823000156581966	28.657332068872293	29.279408816353126	21.240258958192616
76-77	20.853782885700138	28.625409238675676	29.148242613024344	21.372565262599846
78-79	20.89830349156294	28.643460331418	29.30408890371291	21.154147273306144
80-81	20.81484657420103	28.539806291552377	29.34076543400044	21.304581700246153
82-83	20.716638132722352	28.640012439267455	29.420680779421502	21.222668648588694
84-85	20.753447926429036	28.55420143621201	29.2174370454984	21.47491359186055
86-87	20.80046622859695	28.616714671191495	29.191216490956663	21.391602609254893
88-89	20.723333753796293	28.461893926897663	29.239218763644832	21.575553555661212
90-91	20.600605539511253	28.47667018521436	29.204661463194302	21.71806281208008
92-93	20.637599755725635	28.62988340265457	29.13367199010531	21.598844851514478
94-95	20.54251697988418	28.40549947091083	29.2618422248756	21.790141324329387
96-97	20.532171287330577	28.5074209057909	29.179747001867707	21.780660805010818
98-99	20.520635773882002	28.45369144806335	29.17151866715845	21.854154110896204
100	20.592062301511447	28.3832564687983	29.06549188289163	21.959189346798617
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	664.0
1	513.0
2	572.5
3	840.0
4	618.5
5	466.5
6	1229.0
7	3385.0
8	5735.5
9	6807.0
10	5816.5
11	4302.5
12	4275.0
13	4798.5
14	5486.5
15	6375.5
16	7186.0
17	8151.5
18	9442.5
19	11047.5
20	13709.5
21	17554.0
22	23170.5
23	32249.5
24	43882.5
25	57820.5
26	76102.0
27	97988.0
28	124004.0
29	152352.0
30	179163.0
31	204152.5
32	226506.0
33	247452.5
34	266945.5
35	282066.0
36	293203.0
37	304050.5
38	314096.5
39	318183.5
40	317221.0
41	312227.5
42	303253.0
43	294924.0
44	285606.5
45	275303.5
46	264486.5
47	252875.0
48	241845.5
49	234188.5
50	226919.5
51	213288.0
52	198294.0
53	185824.0
54	174291.5
55	162676.0
56	149047.0
57	133064.5
58	118663.5
59	105724.0
60	90057.5
61	73072.0
62	59110.5
63	48281.0
64	38916.0
65	30744.5
66	24127.0
67	18555.0
68	13870.0
69	10429.0
70	7782.0
71	5518.5
72	3978.0
73	2941.5
74	2098.5
75	1386.5
76	886.5
77	570.0
78	345.5
79	196.5
80	105.5
81	56.5
82	32.0
83	19.5
84	12.0
85	7.0
86	5.5
87	3.0
88	1.0
89	1.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	4.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.058084511326600995
2	2.6683216729697683E-4
3	2.425746975427062E-5
4	0.1172727375270213
5	0.0016616366781675373
6	0.0013462895713620194
7	0.0
8	0.0
9	2.425746975427062E-5
10-11	0.0
12-13	1.819310231570296E-5
14-15	1.819310231570296E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.5160918596419137E-4
30-31	2.304459626655709E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.212873487713531E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	9.702987901708248E-5
80-81	2.1831722778843557E-4
82-83	2.971540044898151E-4
84-85	0.0
86-87	6.064367438567655E-6
88-89	3.638620463140592E-5
90-91	4.851493950854124E-5
92-93	6.731447856810097E-4
94-95	6.48887315926739E-4
96-97	0.0
98-99	2.425746975427062E-5
100	9.096551157851483E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8244883.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.4164768067264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.89140281933324	39.994431829532125
2	16.60433701938933	18.735163886908936
3	5.681176767578225	9.615359320289695
4	2.5736961362508675	5.807954735134337
5	1.3559297249509972	3.824838893962442
6	0.7916183800510741	2.679619198675785
7	0.4924520085221713	1.9447685122051837
8	0.33290710089736186	1.5025156589256432
9	0.23124731282711963	1.1741542794656101
>10	0.9776869338669255	9.196600696170277
>50	0.04439912215016366	1.711387533768839
>100	0.021168944652225553	2.2175282613746363
>500	0.0013759786295948937	0.5282222780404128
>1k	5.157865175951886E-4	0.40288577572405004
>5k	2.1491095804688426E-5	0.07094056113803604
>10k+	6.447328741406529E-5	0.5936285786838801
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19213	0.2330293831944007	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18992	0.23034893278655377	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10756	0.1304566723384674	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.212873487713531E-5	0.0	0.0	0.0
6	0.0	1.212873487713531E-5	0.0	0.0	0.0
7	0.0	1.212873487713531E-5	0.0	0.0	0.0
8	0.0	1.212873487713531E-5	0.0	1.212873487713531E-5	0.0
9	0.0	1.212873487713531E-5	0.0	1.212873487713531E-5	0.0
10-11	0.0	1.212873487713531E-5	0.0	1.212873487713531E-5	0.0
12-13	0.0	1.8193102315702965E-5	0.0	2.425746975427062E-5	0.0
14-15	0.0	7.88367767013795E-5	0.0	3.638620463140593E-5	0.0
16-17	0.0	1.3948045108705604E-4	0.0	6.064367438567655E-5	0.0
18-19	0.0	1.4554481852562372E-4	0.0	7.277240926281186E-5	0.0
20-21	0.0	1.4554481852562372E-4	0.0	7.883677670137951E-5	0.0
22-23	0.0	1.4554481852562372E-4	0.0	1.8193102315702966E-4	0.0
24-25	0.0	1.4554481852562372E-4	0.0	3.2747584168265335E-4	0.0
26-27	0.0	1.4554481852562372E-4	0.0	8.368827065223362E-4	0.0
28-29	0.0	1.4554481852562372E-4	0.0	0.0024378757103041973	0.0
30-31	0.0	1.5767355340275902E-4	0.0	0.008289990288521985	0.0
32-33	0.0	1.9405975803416496E-4	0.0	0.021370830853512414	0.0
34-35	0.0	2.2438159522700322E-4	0.0	0.04234141345607936	0.0
36-37	0.0	2.3651033010413852E-4	0.0	0.07604110331219982	0.0
38-39	0.0	2.425746975427062E-4	0.0	0.11944378107002851	0.0
40-41	0.0	2.425746975427062E-4	0.0	0.16552690923570412	0.0
42-43	0.0	2.425746975427062E-4	0.0	0.21233776149400785	0.0
44-45	0.0	2.486390649812738E-4	0.0	0.27175643365709373	0.0
46-47	0.0	2.668321672969768E-4	0.0	0.3431582958787893	0.0
48-49	0.0	2.668321672969768E-4	0.0	0.41876882910285085	0.0
50-51	0.0	2.971540044898151E-4	0.0	0.49633815300956974	0.0
52-53	0.0	3.0321837192838274E-4	0.0	0.5724338356287166	0.0
54-55	0.0	3.0321837192838274E-4	0.0	0.6597061474371437	0.0
56-57	0.0	3.0321837192838274E-4	0.0	0.765953864960849	0.0
58-59	0.0	3.0321837192838274E-4	0.0	0.8809767221681617	0.0
60-61	0.0	3.0321837192838274E-4	0.0	0.9976248298490107	0.0
62-63	0.0	3.0321837192838274E-4	0.0	1.1150734340317503	0.0
64-65	0.0	3.0321837192838274E-4	0.0	1.243492478910859	0.0
66-67	0.0	3.0321837192838274E-4	0.0	1.3887401434319928	0.0
68-69	0.0	3.0321837192838274E-4	0.0	1.5536970021284717	0.0
70-71	0.0	3.0321837192838274E-4	0.0	1.7300973221815275	0.0
72-73	0.0	3.33540209121221E-4	0.0	1.9100756190233386	0.0
74-75	0.0	3.5173331143692395E-4	0.0	2.096949101642801	0.0
76-77	0.0	3.5173331143692395E-4	0.0	2.3001296683045713	0.0
78-79	0.0	3.699264137526269E-4	0.0	2.528168077097031	0.0
80-81	0.0	3.881195160683299E-4	0.0	2.7741206273030192	0.0
82-83	0.0	4.002482509454652E-4	0.0	3.023529866948991	0.0
84-85	0.0	4.123769858226005E-4	0.0	3.280804591162785	0.0
86-87	0.0	4.3663445557687113E-4	0.0	3.5587042290351483	0.0
88	0.0	4.851493950854124E-4	0.0	3.7836194885967456	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14090	0.0	55.470688	1
GTATCAA	25405	0.0	38.97504	1
ATCAACG	30790	0.0	31.726372	3
TCAACGC	31015	0.0	31.480846	4
TATCAAC	32455	0.0	30.127748	2
CAACGCA	32490	0.0	30.088633	5
GTGGTAT	6360	0.0	29.68614	1
AACGCAG	34040	0.0	28.73184	6
TGGTATC	6315	0.0	28.538303	2
ACGCAGA	38295	0.0	25.268803	7
CGCAGAG	38210	0.0	25.140518	8
GCAGAGT	41970	0.0	22.451523	9
GTACATG	19760	0.0	21.420975	1
GAGTACT	29905	0.0	21.137268	12-13
CAGAGTA	41195	0.0	20.1644	10-11
AGAGTAC	38690	0.0	20.024448	10-11
TACATGG	20455	0.0	20.013474	2
GTACTTT	32845	0.0	19.102154	14-15
AGTACTT	31775	0.0	18.828392	12-13
ACATGGG	20830	0.0	18.77217	3
>>END_MODULE