FastQCFastQC Report
Wed 25 May 2016
SRR2049514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5906321
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57500.046.3074951
GTATCAA92150.035.7298661
TCAACGC96650.033.0533334
ATCAACG96850.032.9322173
CAACGCA104200.030.756075
TATCAAC109600.029.5682542
AACGCAG114450.027.8671346
GTGGTAT35700.023.8097931
ACGCAGA133050.023.7020477
CGCAGAG132400.023.6074268
GTCTCGC19650.023.4528561
GTACATG140400.023.4144381
TCTGTCG19250.023.0278768
TACATGG139950.022.0219692
TACCTGG37400.021.6280822
TGGTATC35400.020.9699442
TCGCTCT21700.020.525424
ACATGGG144950.020.2380643
CGCTCTG19650.019.8004595
GCAGAGT149400.019.770669
Produced by FastQC (version 0.11.5)