##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049514_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5906321 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.76152667625075 31.0 31.0 34.0 28.0 34.0 2 30.947685538933627 31.0 31.0 34.0 27.0 34.0 3 31.057596767937266 31.0 31.0 34.0 27.0 34.0 4 34.63755762682049 37.0 35.0 37.0 32.0 37.0 5 34.65804415303537 37.0 35.0 37.0 32.0 37.0 6 34.64983819877044 37.0 35.0 37.0 32.0 37.0 7 30.680069708368375 37.0 33.0 37.0 2.0 37.0 8 32.44845446090722 37.0 33.0 37.0 17.0 37.0 9 35.22814811453695 39.0 34.0 39.0 28.0 39.0 10-11 35.75172421207719 39.0 36.0 39.0 30.0 39.0 12-13 35.90472647863196 39.0 37.0 39.0 30.0 39.0 14-15 37.08614889031598 40.0 37.0 41.0 31.0 41.0 16-17 37.10214378798579 40.0 37.0 41.0 31.0 41.0 18-19 37.112004494845436 40.0 37.0 41.0 31.0 41.0 20-21 37.183418916784234 40.0 37.0 41.0 31.0 41.0 22-23 37.14022290695003 40.0 37.0 41.0 31.0 41.0 24-25 37.11980613651036 40.0 37.0 41.0 31.0 41.0 26-27 37.1417066563094 40.0 37.0 41.0 31.0 41.0 28-29 37.109874065429224 40.0 37.0 41.0 31.0 41.0 30-31 37.01246368085988 40.0 37.0 41.0 30.0 41.0 32-33 36.93008346481676 40.0 37.0 41.0 30.0 41.0 34-35 36.851887748735635 40.0 37.0 41.0 30.0 41.0 36-37 36.66527538885882 40.0 37.0 41.0 30.0 41.0 38-39 36.52971587558482 40.0 36.0 41.0 30.0 41.0 40-41 36.43033827656844 40.0 36.0 41.0 30.0 41.0 42-43 36.30555704642535 40.0 36.0 41.0 29.5 41.0 44-45 36.18411410419447 40.0 36.0 41.0 29.0 41.0 46-47 36.015545209953885 40.0 35.0 41.0 29.0 41.0 48-49 35.918011990882306 39.0 35.0 41.0 28.0 41.0 50-51 35.7748945578813 39.0 35.0 41.0 28.0 41.0 52-53 35.6350068849966 39.0 35.0 41.0 28.0 41.0 54-55 35.49178270195609 39.0 35.0 41.0 28.0 41.0 56-57 35.283186860314565 39.0 35.0 41.0 27.0 41.0 58-59 35.084222225646045 39.0 34.5 40.0 27.0 41.0 60-61 34.83733215651503 38.0 34.0 40.0 26.0 41.0 62-63 34.559360556258284 38.0 34.0 40.0 26.0 41.0 64-65 34.169657050471855 37.0 34.0 40.0 25.0 41.0 66-67 30.70705190252951 36.0 30.0 39.5 6.5 41.0 68-69 31.02470598871954 35.5 29.5 39.0 11.0 41.0 70-71 31.14734502239211 35.0 29.0 39.0 14.0 41.0 72-73 32.32132870868346 35.0 31.5 39.0 20.5 40.0 74-75 32.37457344428113 35.0 32.0 38.0 21.0 40.0 76-77 32.04650407588751 35.0 32.0 37.0 20.0 39.5 78-79 28.72938136955306 35.0 28.0 36.0 6.0 39.0 80-81 30.408826408182016 35.0 29.0 36.0 11.0 38.5 82-83 30.74098893372033 35.0 31.0 36.0 8.5 37.0 84-85 28.895855389505584 34.5 28.5 35.5 3.5 37.0 86-87 28.465420267540487 34.0 26.0 35.0 2.0 37.0 88-89 29.593381565275575 34.0 30.0 35.0 2.0 36.0 90-91 29.650816726689932 34.5 31.0 35.0 2.0 36.0 92-93 29.408293165914955 34.0 31.0 35.0 2.0 36.0 94-95 29.109198010064134 34.0 30.5 35.0 2.0 35.5 96-97 28.819641279232876 34.0 30.0 35.0 2.0 35.0 98-99 28.53557519139241 34.0 30.0 35.0 2.0 35.0 100 28.25683111364926 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.21773116175630847 1101 2 0.2266883755924809 1101 3 0.29468685777980497 1101 4 0.2355338589409186 1101 5 0.2413439794271781 1101 6 0.2589277848071845 1101 7 -12.196670030833737 1101 8 -6.60035126390526 1101 9 -2.272783553061892 1101 10-11 -0.19763868561194897 1101 12-13 0.27183846388126653 1101 14-15 0.506084676535032 1101 16-17 0.5451833885417585 1101 18-19 0.5035149956997387 1101 20-21 0.5177629329154811 1101 22-23 0.45444150216452783 1101 24-25 0.4867737850079692 1101 26-27 0.42527391524524916 1101 28-29 0.3480049281958202 1101 30-31 0.4508870664141469 1101 32-33 0.4375208320308559 1101 34-35 0.41120361965103314 1101 36-37 0.5230922702393457 1101 38-39 0.4640723313114705 1101 40-41 0.39080879401881674 1101 42-43 0.4393981193243377 1101 44-45 0.32086507980096 1101 46-47 0.3275669749686756 1101 48-49 0.29226553116895104 1101 50-51 0.44271241646478643 1101 52-53 0.4660712988318565 1101 54-55 0.344939657836818 1101 56-57 0.28816742502534964 1101 58-59 0.279553556754891 1101 60-61 0.25224204351768975 1101 62-63 0.3267498134794735 1101 64-65 0.18113268629961254 1101 66-67 -10.277952706820631 1101 68-69 -8.149369145941659 1101 70-71 -6.719550316676045 1101 72-73 -1.6912252934637735 1101 74-75 -0.43081229528085174 1101 76-77 -0.12782486242038615 1101 78-79 2.911129234428948 1101 80-81 0.9013313137304628 1101 82-83 0.21862571718158819 1101 84-85 -5.423441651418784 1101 86-87 -5.910013296288074 1101 88-89 -1.4962906103057776 1101 90-91 -0.25862819065296705 1101 92-93 0.11085864970064208 1101 94-95 0.16513530787734254 1101 96-97 0.2353205221937067 1101 98-99 0.29038599825148736 1101 100 0.34497720938551524 1102 1 0.14846278938660262 1102 2 0.17133598266345373 1102 3 0.18565642433538088 1102 4 0.20010729888399936 1102 5 0.142636532288428 1102 6 0.19039597603015324 1102 7 -12.352164910598358 1102 8 -6.749615426836929 1102 9 -2.3840201107102814 1102 10-11 -0.3684715951394182 1102 12-13 0.17595930997831033 1102 14-15 0.29713048411527865 1102 16-17 0.3132704983706631 1102 18-19 0.2975943286359026 1102 20-21 0.34926322330506565 1102 22-23 0.36593685659172337 1102 24-25 0.39327012354542035 1102 26-27 0.35246439715985645 1102 28-29 0.34101221941803317 1102 30-31 0.31436541400717743 1102 32-33 0.3061626542067586 1102 34-35 0.3305980770188057 1102 36-37 0.5090029376862617 1102 38-39 0.4933814903177094 1102 40-41 0.3597434055066131 1102 42-43 0.3201281397947042 1102 44-45 0.3749651649143857 1102 46-47 0.3722417056152807 1102 48-49 0.2775869213226514 1102 50-51 0.38793065337073074 1102 52-53 0.39453522381580086 1102 54-55 0.37774381725475337 1102 56-57 0.45550355085407546 1102 58-59 0.36809567337130034 1102 60-61 0.2880709659675986 1102 62-63 0.31394999559490344 1102 64-65 0.25166562933526393 1102 66-67 -10.203730429118366 1102 68-69 -6.661324104074044 1102 70-71 -6.189099875983992 1102 72-73 -1.1091750681751797 1102 74-75 -0.19531810241197434 1102 76-77 -2.2658018813359604E-4 1102 78-79 3.0440932512191488 1102 80-81 0.9967533297078361 1102 82-83 0.3719997182656485 1102 84-85 -5.390255956639148 1102 86-87 -5.813897713749842 1102 88-89 -1.43031015689294 1102 90-91 -0.11599640243877829 1102 92-93 0.20016086489539475 1102 94-95 0.20871895306574828 1102 96-97 0.33377064761822695 1102 98-99 0.4014788141585832 1102 100 0.4741712473065647 1103 1 0.3011214717999735 1103 2 0.2980758406014665 1103 3 0.2786832365136469 1103 4 0.34440020957868 1103 5 0.3100837521255997 1103 6 0.3416544255392324 1103 7 -12.151636757725754 1103 8 -6.56995573437899 1103 9 -2.1367469634719143 1103 10-11 -0.21078670156744295 1103 12-13 0.3588651143520636 1103 14-15 0.5212346511411283 1103 16-17 0.5381307761972351 1103 18-19 0.5788767529891317 1103 20-21 0.531655999140277 1103 22-23 0.549857125214352 1103 24-25 0.607125242026882 1103 26-27 0.5944516807169222 1103 28-29 0.5669744866402908 1103 30-31 0.6055572777565104 1103 32-33 0.6754676438235521 1103 34-35 0.6406142014990621 1103 36-37 0.7046831632598156 1103 38-39 0.7617122333387769 1103 40-41 0.728909711856609 1103 42-43 0.672524156397273 1103 44-45 0.6179856080973352 1103 46-47 0.6173232293907347 1103 48-49 0.5729967177030346 1103 50-51 0.6247949729570763 1103 52-53 0.5088798985357883 1103 54-55 0.5064668889752326 1103 56-57 0.5554220311029283 1103 58-59 0.5121708337572315 1103 60-61 0.46463552862349644 1103 62-63 0.38749222180792486 1103 64-65 0.29081639635334255 1103 66-67 -10.19649634194479 1103 68-69 -8.139332890251701 1103 70-71 -6.645858224412358 1103 72-73 -1.5775605643638286 1103 74-75 -0.34970459631811224 1103 76-77 -4.589592057797631E-4 1103 78-79 3.0517402040484924 1103 80-81 0.9382324576679046 1103 82-83 0.2990399998982305 1103 84-85 -5.361806605571356 1103 86-87 -5.808968904940617 1103 88-89 -1.3372480155354722 1103 90-91 -0.04742872799103637 1103 92-93 0.3148119800019096 1103 94-95 0.40859184285860195 1103 96-97 0.540910722744556 1103 98-99 0.5699333815754457 1103 100 0.5955480737118144 1104 1 0.09474985925693602 1104 2 0.18348994082777992 1104 3 0.11909217134174455 1104 4 0.1509993840450008 1104 5 0.19433396133282343 1104 6 0.21868623251744168 1104 7 -12.103765077687818 1104 8 -6.653076450365386 1104 9 -2.417030872008006 1104 10-11 -0.3706398477697377 1104 12-13 0.17175902524761 1104 14-15 0.3426742583053368 1104 16-17 0.3459196669888769 1104 18-19 0.3617707292566763 1104 20-21 0.37531916517051656 1104 22-23 0.3812910247971857 1104 24-25 0.45975950076476835 1104 26-27 0.4832295472972632 1104 28-29 0.3798634980061877 1104 30-31 0.3423275536377659 1104 32-33 0.3701149447454526 1104 34-35 0.31549036369682426 1104 36-37 0.37524079106256636 1104 38-39 0.3926798014852082 1104 40-41 0.4416759991028911 1104 42-43 0.3629001861998944 1104 44-45 0.36246738646350707 1104 46-47 0.39904115005730745 1104 48-49 0.33180978878549894 1104 50-51 0.4237964595970283 1104 52-53 0.4170966456403349 1104 54-55 0.38500320874374694 1104 56-57 0.36816212911644186 1104 58-59 0.34725513228779903 1104 60-61 0.34976473501628647 1104 62-63 0.2789645331640642 1104 64-65 0.12828907743190854 1104 66-67 -10.12669345736861 1104 68-69 -8.116382253316214 1104 70-71 -6.595152358543146 1104 72-73 -1.6524768932695935 1104 74-75 -0.45068225129538675 1104 76-77 -0.14432603193360194 1104 78-79 2.8330623882898145 1104 80-81 0.8102887552180285 1104 82-83 0.14674405132402057 1104 84-85 -5.4004191585167725 1104 86-87 -5.833830911069544 1104 88-89 -1.5237506335489321 1104 90-91 -0.23513642137806556 1104 92-93 0.12864113495301055 1104 94-95 0.16173342194533546 1104 96-97 0.26733870481429634 1104 98-99 0.3391223355420401 1104 100 0.4221547841534523 1105 1 0.01586533855580541 1105 2 0.036835278459300014 1105 3 0.03362988307268111 1105 4 0.06035857921558829 1105 5 0.038989138447462324 1105 6 0.0802948123420606 1105 7 -12.335055765983878 1105 8 -6.785978881989905 1105 9 -2.5719642846996535 1105 10-11 -0.5264185115606352 1105 12-13 0.044988782835424956 1105 14-15 0.11612793627494966 1105 16-17 0.14775051583082188 1105 18-19 0.15892135206665614 1105 20-21 0.18013942845679054 1105 22-23 0.19052282899408368 1105 24-25 0.238584792784593 1105 26-27 0.19710131826577282 1105 28-29 0.1909560844432292 1105 30-31 0.20850641033278094 1105 32-33 0.16322142623499758 1105 34-35 0.18168242744026486 1105 36-37 0.2973387742128466 1105 38-39 0.22727642864094122 1105 40-41 0.25763558592144165 1105 42-43 0.23489522978604782 1105 44-45 0.15274284140571126 1105 46-47 0.1271722792251424 1105 48-49 0.1679330385857014 1105 50-51 0.24471953753156583 1105 52-53 0.22317792100420064 1105 54-55 0.22798070397190173 1105 56-57 0.1789411496716724 1105 58-59 0.15009045160928736 1105 60-61 0.09256286189715013 1105 62-63 0.047204114823557575 1105 64-65 -0.013993123740249303 1105 66-67 -10.313071924194649 1105 68-69 -8.369263255876216 1105 70-71 -6.76983815938047 1105 72-73 -1.8253414750015935 1105 74-75 -0.5964951238554903 1105 76-77 -0.3222055407242195 1105 78-79 2.731745821526829 1105 80-81 0.6860506501181511 1105 82-83 0.05239312941209562 1105 84-85 -5.5575328944079025 1105 86-87 -6.071523053803897 1105 88-89 -1.6993570024014915 1105 90-91 -0.4140359667887523 1105 92-93 -0.07991161811714775 1105 94-95 0.009927101860238707 1105 96-97 0.13560756242008054 1105 98-99 0.1721406720688492 1105 100 0.2055320797482736 1106 1 0.03405833825813076 1106 2 0.05488324668554867 1106 3 0.10691660143433168 1106 4 0.13220651927004212 1106 5 0.07768598015515948 1106 6 0.15461763835047293 1106 7 -12.302898702620052 1106 8 -6.713132138386143 1106 9 -2.644248523742071 1106 10-11 -0.5342368307212055 1106 12-13 0.04965961844609268 1106 14-15 0.19298506904502233 1106 16-17 0.20782600794905193 1106 18-19 0.23007277097531897 1106 20-21 0.2028489435237688 1106 22-23 0.15371650654066826 1106 24-25 0.2184108821750783 1106 26-27 0.1734430882237561 1106 28-29 0.1719324290383213 1106 30-31 0.15729910432281002 1106 32-33 0.09473662938703598 1106 34-35 0.16730180342690915 1106 36-37 0.23025139705139708 1106 38-39 0.18924588232231798 1106 40-41 0.17710263355682088 1106 42-43 0.1745115069744685 1106 44-45 0.12337207994253419 1106 46-47 0.1325181774328641 1106 48-49 0.11243425256459716 1106 50-51 0.13993598493296133 1106 52-53 0.12775316424424688 1106 54-55 0.066371933972313 1106 56-57 0.06966065269962485 1106 58-59 0.006643406222323733 1106 60-61 -0.002076180414768203 1106 62-63 -0.037776612282726774 1106 64-65 -0.13940239619522998 1106 66-67 -10.524601735303417 1106 68-69 -8.476083706573544 1106 70-71 -6.949921895406199 1106 72-73 -1.959191563220692 1106 74-75 -0.770225167389647 1106 76-77 -0.42171343212414314 1106 78-79 2.6537848587746353 1106 80-81 0.5965346562533576 1106 82-83 -0.04300840542302353 1106 84-85 -5.630229092880704 1106 86-87 -6.091652762038297 1106 88-89 -1.6489118287886058 1106 90-91 -0.38550039364985267 1106 92-93 -0.04454281973862706 1106 94-95 -0.015094466278593899 1106 96-97 0.017517927603226013 1106 98-99 0.028258960679551137 1106 100 0.09232484842100774 1107 1 0.05629710969725821 1107 2 0.05878891394301888 1107 3 0.027945266038216232 1107 4 8.806649127279798E-4 1107 5 0.06169446227765718 1107 6 0.07985928386368357 1107 7 -12.342071851235556 1107 8 -6.8057521130561085 1107 9 -2.6531448584231967 1107 10-11 -0.5809258845241487 1107 12-13 0.023412931080734722 1107 14-15 0.12962745320280789 1107 16-17 0.14090138582225364 1107 18-19 0.21297827547552117 1107 20-21 0.182731644759194 1107 22-23 0.22629898495950584 1107 24-25 0.2773588943430809 1107 26-27 0.23723886002269268 1107 28-29 0.19251996177334973 1107 30-31 0.22884712718670386 1107 32-33 0.22828163372916066 1107 34-35 0.14883387134378978 1107 36-37 0.2198212357183138 1107 38-39 0.23760903766067543 1107 40-41 0.25051835529087185 1107 42-43 0.3078022846034969 1107 44-45 0.25195048744886606 1107 46-47 0.3001492538009529 1107 48-49 0.3141287374071595 1107 50-51 0.3491493822767566 1107 52-53 0.31626502871308304 1107 54-55 0.27807215034431465 1107 56-57 0.2364738061442324 1107 58-59 0.22867786994171269 1107 60-61 0.17170938173502748 1107 62-63 0.15717372984088485 1107 64-65 0.019759510863941898 1107 66-67 -10.401957174575099 1107 68-69 -8.377318280140795 1107 70-71 -6.842544856858083 1107 72-73 -1.9358442162076983 1107 74-75 -0.5840030281590884 1107 76-77 -0.31021903075205515 1107 78-79 2.727206039568255 1107 80-81 0.695585843922494 1107 82-83 0.03215350883438006 1107 84-85 -5.628989922592055 1107 86-87 -6.1265349690597475 1107 88-89 -1.7092524748301408 1107 90-91 -0.435307240769518 1107 92-93 -0.1234204392659457 1107 94-95 -0.029627394697858023 1107 96-97 0.014696354694539338 1107 98-99 0.12284711352510058 1107 100 0.20299453927784583 1108 1 0.11814303709854812 1108 2 0.1495159967487929 1108 3 0.07675752620145104 1108 4 0.04916485808723792 1108 5 0.07431815474118508 1108 6 0.1419919196819066 1108 7 -12.225632928041758 1108 8 -6.713296257630198 1108 9 -2.572455005101034 1108 10-11 -0.5454912199547408 1108 12-13 0.04548065270451929 1108 14-15 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2216 8 1.2232126458691752 2216 9 -0.05466749368267898 2216 10-11 -0.7230970751756018 2216 12-13 -1.1039513953822748 2216 14-15 -1.3694455593109893 2216 16-17 -1.4858224594114589 2216 18-19 -1.9860588748741321 2216 20-21 -1.5931544888440428 2216 22-23 -1.4233225875469344 2216 24-25 -1.4067310026020863 2216 26-27 -1.860344296951908 2216 28-29 -2.0250450685910266 2216 30-31 -1.9304890662251424 2216 32-33 -2.8116023605660203 2216 34-35 -1.8395787850787357 2216 36-37 -1.5172598279548595 2216 38-39 -1.773845668817053 2216 40-41 -1.8368131454452268 2216 42-43 -1.4737382472330864 2216 44-45 -1.584849912808771 2216 46-47 -1.763971743622676 2216 48-49 -1.8559865091883552 2216 50-51 -2.3977711642819486 2216 52-53 -2.160511954172442 2216 54-55 -1.763170810642066 2216 56-57 -1.7879054494685818 2216 58-59 -2.1158183345117294 2216 60-61 -2.2219414279815766 2216 62-63 -2.044002130483001 2216 64-65 -1.7516276118817373 2216 66-67 1.5584220120277728 2216 68-69 0.7796669057259713 2216 70-71 0.39472145249046164 2216 72-73 -1.3039316748017349 2216 74-75 -2.061818978655957 2216 76-77 -1.7606640758385481 2216 78-79 0.7256406411508785 2216 80-81 -0.6699150072000677 2216 82-83 -1.5345823671317618 2216 84-85 0.22119037737114056 2216 86-87 0.2158223183896446 2216 88-89 -1.068768600152186 2216 90-91 -1.2682287304640347 2216 92-93 -1.3216964560123579 2216 94-95 -1.4345164497217837 2216 96-97 -1.3361550899134862 2216 98-99 -1.300858973151378 2216 100 -2.3435993602436866 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 86921.0 3 16730.0 4 10058.0 5 9237.0 6 8907.0 7 10867.0 8 13092.0 9 14654.0 10 16199.0 11 18553.0 12 20916.0 13 22985.0 14 25847.0 15 26860.0 16 27615.0 17 28314.0 18 26621.0 19 25463.0 20 25478.0 21 25120.0 22 27220.0 23 31324.0 24 37510.0 25 46392.0 26 56522.0 27 69643.0 28 87239.0 29 110130.0 30 134834.0 31 161217.0 32 212109.0 33 291122.0 34 430179.0 35 570236.0 36 647761.0 37 1060603.0 38 1113263.0 39 357728.0 40 852.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.423629287034174 20.18038869832393 13.429766201182405 22.96621581345949 2 16.71599341301691 20.542332337881213 39.070287904528705 23.671386344573172 3 18.449136202297094 24.99732486500727 30.343869978957862 26.209668953737776 4 13.221553564840455 17.555137425995106 38.1990136800381 31.02429532912634 5 12.938878791239397 36.67372420407994 34.48425091196307 15.903146092717588 6 30.13571341736591 37.72363659267452 18.127986519750184 14.012663470209386 7 26.78040627548611 32.19978890599035 22.483811526928665 18.53599329159487 8 24.498134963587304 35.076567994380255 21.486648452276285 18.938648589756156 9 24.19257266917934 17.643097962335606 22.426244696148416 35.73808467233664 10-11 23.252638994731235 26.937885699067156 28.929810960156075 20.879664346045534 12-13 24.1845558343341 24.67291398486469 28.629395523880262 22.51313465692095 14-15 21.93617651326435 27.348361187954396 25.941622543034825 24.77383975574643 16-17 21.1368462293131 30.237993994383462 26.364565186234472 22.26059459006897 18-19 20.902648196737022 28.946471077342395 28.559072559720338 21.591808166200245 20-21 22.456106340326972 28.727778041149143 27.64166181310867 21.174453805415215 22-23 21.98782643102419 28.936755125293317 27.63380348221875 21.44161496146374 24-25 21.67288232386963 28.639909683202113 27.9625252335591 21.724682759369156 26-27 21.42915978243267 28.384128870998037 28.05513324352542 22.13157810304387 28-29 21.591791235186843 28.48464382481074 28.05959073338547 21.863974206616945 30-31 22.20367993914316 29.277398678246463 27.27451041642593 21.244410966184446 32-33 21.251171838161806 28.887510139564082 28.119409060025376 21.741908962248736 34-35 21.313094543721423 28.042942172911534 28.28397232453738 22.35999095882966 36-37 22.259193924337644 28.80233018230136 27.381665297793774 21.55681059556722 38-39 21.97442636850911 28.781103557241583 27.736070086304192 21.50839998794511 40-41 21.45779254613765 28.457311112730842 28.093963068885273 21.99093327224623 42-43 21.3597353410589 28.427149670306466 28.013016907232487 22.200098081402146 44-45 21.807614688599486 28.681222797623928 27.819897995507688 21.6912645182689 46-47 21.449512276164494 29.245872495507946 27.53800732696862 21.766607901358938 48-49 21.25001079360145 28.464022259707768 28.20086155796621 22.08510538872457 50-51 21.304374621378482 28.118579942007628 28.19206087607559 22.3849845605383 52-53 21.222796515075647 28.304136390014218 28.080730779308016 22.39233631560212 54-55 21.45142661999661 28.930492777567217 27.78858218079705 21.829498421639116 56-57 20.98912002847168 28.59565243045911 27.817167987068547 22.59805955400066 58-59 20.733922710696792 27.932045999438394 28.384343503649795 22.949687786215016 60-61 21.46184382378866 28.469863835213893 27.611972518491502 22.456319822505947 62-63 21.26394613489658 28.396666769102062 27.708521008103148 22.630866087898205 64-65 21.103529113774886 27.945798699071254 28.180059333836983 22.77061285331688 66-67 20.99474093254468 28.08379415118637 27.7087487345824 23.212716181686552 68-69 21.779429527283227 28.255430534978593 27.483843245517026 22.481296692221157 70-71 21.539293524527935 27.798974088765565 27.755951240094245 22.90578114661225 72-73 21.004880172204125 27.858971006374354 28.364012575499515 22.77213624592201 74-75 20.977552964083895 27.7541365954835 28.225418527376238 23.04289191305636 76-77 21.040081808905363 27.911396600568732 27.842847185600778 23.205674404925126 78-79 21.307349100370555 27.98831851636171 27.940942039136214 22.763390344131523 80-81 20.927367924325438 27.634677254269242 28.225937673498613 23.212017147906707 82-83 20.848349434555903 27.536904496537986 28.37589568544578 23.23885038346034 84-85 21.068263848544703 27.454971841642262 28.122204153419567 23.35456015639347 86-87 21.288229819693235 27.506371195685254 28.08495356517321 23.120445419448302 88-89 20.99927068258269 27.269885116633642 28.29491683114757 23.4359273696361 90-91 20.953000892382224 27.039545459894317 28.241607429198435 23.765846218525027 92-93 21.31617172847489 27.261652419510636 27.861600845635465 23.560575006379008 94-95 21.31454089035977 26.899658351029842 28.08624426902393 23.699556489586463 96-97 21.250901501858284 26.800123228182173 28.25498785877319 23.693987411186352 98-99 21.326871619838037 26.73504609829347 28.22088237201461 23.717199909853882 100 21.636324138492714 26.86487558239926 27.874076970082918 23.624723309025107 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3544.0 1 2746.0 2 2265.0 3 2569.5 4 2359.5 5 2079.5 6 1895.5 7 1800.0 8 1785.0 9 1797.0 10 1701.0 11 1572.5 12 1575.5 13 1651.5 14 1782.0 15 1989.5 16 2292.0 17 2737.0 18 3523.5 19 4652.5 20 6460.0 21 8827.5 22 12352.5 23 17739.5 24 24471.0 25 32512.5 26 43189.5 27 56851.5 28 73732.5 29 90760.0 30 104858.5 31 118732.5 32 132402.5 33 145059.0 34 157938.5 35 169690.0 36 178517.0 37 185706.5 38 191159.0 39 194725.5 40 197008.5 41 196304.5 42 193916.5 43 192531.0 44 191258.0 45 190608.5 46 190561.5 47 189697.5 48 188532.5 49 189591.0 50 188993.0 51 183964.0 52 177880.0 53 172636.5 54 168160.5 55 161412.0 56 150584.5 57 136320.0 58 121643.0 59 107406.0 60 91751.5 61 75540.5 62 61123.0 63 48301.0 64 37472.0 65 28759.0 66 21620.0 67 16160.5 68 11954.0 69 8905.5 70 6578.0 71 4870.0 72 3780.0 73 2818.0 74 2051.5 75 1475.0 76 1019.5 77 741.0 78 529.0 79 372.0 80 270.0 81 183.0 82 141.5 83 120.5 84 102.5 85 76.5 86 52.5 87 40.0 88 35.0 89 31.5 90 28.5 91 22.5 92 15.5 93 12.5 94 9.5 95 9.5 96 11.5 97 12.5 98 14.5 99 19.0 100 31.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.018166977378980924 2 0.00215023870189243 3 0.0013036880318560403 4 0.0020994456616902466 5 0.0017946874204771463 6 0.0033692716667448317 7 11.645032499926772 8 3.386202680145559E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.7936172111200864E-4 18-19 0.0 20-21 1.6931013400727794E-5 22-23 8.465506700363897E-6 24-25 0.0 26-27 1.9470665410836966E-4 28-29 0.0 30-31 8.465506700363897E-6 32-33 3.386202680145559E-5 34-35 1.0158608040436677E-4 36-37 3.386202680145559E-5 38-39 5.0793040202183386E-5 40-41 8.465506700363897E-6 42-43 4.2327533501819494E-5 44-45 2.1163766750909745E-4 46-47 9.904642839425762E-4 48-49 7.449645896320231E-4 50-51 4.5713736181965056E-4 52-53 3.978788149171032E-4 54-55 6.010509757258368E-4 56-57 0.0017184978601738714 58-59 5.333269221229255E-4 60-61 0.0016084462730691408 62-63 0.0027004966374160836 64-65 0.0013375500586574958 66-67 5.82304957688551 68-69 5.473534201747586 70-71 0.002658169103914264 72-73 0.0012613604983542207 74-75 0.0033184786265426486 76-77 0.006137492357763826 78-79 5.4215729216207516 80-81 0.004977717939813973 82-83 0.005070838513517975 84-85 5.8232612145530185 86-87 0.004190425816680129 88-89 0.0019216700209826049 90-91 0.0037163574414597516 92-93 0.0038941330821673933 94-95 0.004385132470788499 96-97 0.0034623922404488344 98-99 0.00599357874385764 100 0.006162888877864917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5906321.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.88830866162215 #Duplication Level Percentage of deduplicated Percentage of total 1 93.24290443488964 83.81446974349495 2 4.885928217296354 8.783756473897279 3 1.0061270399580902 2.7131717376167113 4 0.38079695493230054 1.3691677688944182 5 0.17444239802592168 0.7840166058713797 6 0.104973600340305 0.566153963322666 7 0.06073627827906354 0.38216369302346487 8 0.04050310680207347 0.2912604612783542 9 0.023904017867591054 0.193382256270146 >10 0.07885707293578403 1.0330671801918652 >50 6.984659644807438E-4 0.04407657465620571 >100 1.0875797230610785E-4 0.012946424557063747 >500 1.965473614597302E-5 0.012367116925463752 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.3862026801455595E-5 0.0 6 0.0 0.0 0.0 3.3862026801455595E-5 0.0 7 0.0 1.6931013400727797E-5 0.0 3.3862026801455595E-5 0.0 8 0.0 1.6931013400727797E-5 0.0 3.3862026801455595E-5 0.0 9 0.0 1.6931013400727797E-5 0.0 6.772405360291119E-5 0.0 10-11 0.0 1.6931013400727797E-5 0.0 1.0158608040436678E-4 0.0 12-13 0.0 2.5396520101091696E-5 0.0 1.5237912060655018E-4 0.0 14-15 0.0 7.618956030327509E-5 0.0 1.8624114740800577E-4 0.0 16-17 0.0 1.1851709380509458E-4 0.0 2.2010317420946137E-4 0.0 18-19 0.0 1.3544810720582238E-4 0.0 2.2856868090982528E-4 0.0 20-21 0.0 1.3544810720582238E-4 0.0 3.8094780151637543E-4 0.0 22-23 0.0 1.3544810720582238E-4 0.0 0.0010497228308451235 0.0 24-25 0.0 1.608446273069141E-4 0.0 0.002573514036910625 0.0 26-27 0.0 1.8624114740800577E-4 0.0 0.004893062872810333 0.0 28-29 0.0 1.8624114740800577E-4 0.0 0.013815706934993881 0.0 30-31 0.0 1.8624114740800577E-4 0.0 0.03908524443558012 0.0 32-33 0.0 1.8624114740800577E-4 0.0 0.09863161856593977 0.0 34-35 0.0 1.8624114740800577E-4 0.0 0.19544315319130132 0.0 36-37 0.0 2.0317216080873357E-4 0.0 0.34123610958496836 0.0 38-39 0.0 2.2856868090982528E-4 0.0 0.5407003784589426 0.0 40-41 0.0 2.3703418761018916E-4 0.0 0.780883734561667 0.0 42-43 0.0 2.3703418761018916E-4 0.0 1.033917729835544 0.0 44-45 0.0 2.3703418761018916E-4 0.0 1.3297871890132622 0.0 46-47 0.0 2.3703418761018916E-4 0.0 1.681427406332978 0.0 48-49 0.0 2.3703418761018916E-4 0.0 2.0597255042521394 0.0 50-51 0.0 2.3703418761018916E-4 0.0 2.452550411669125 0.0 52-53 0.0 2.3703418761018916E-4 0.0 2.837807156095986 0.0 54-55 0.0 2.3703418761018916E-4 0.0 3.2582804083963604 0.0 56-57 0.0 2.3703418761018916E-4 0.0 3.6977502577323516 0.0 58-59 0.0 2.3703418761018916E-4 0.0 4.161710817952495 0.0 60-61 0.0 2.3703418761018916E-4 0.0 4.633925247205494 0.0 62-63 0.0 2.3703418761018916E-4 0.0 5.095803292777348 0.0 64-65 0.0 2.3703418761018916E-4 0.0 5.61043837610587 0.0 66-67 0.0 2.3703418761018916E-4 0.0 6.174876035352634 0.0 68-69 0.0 2.3703418761018916E-4 0.0 6.698518417810343 0.0 70-71 0.0 2.3703418761018916E-4 0.0 7.305977782108355 0.0 72-73 0.0 2.4549969431055305E-4 0.0 7.982024681692716 0.0 74-75 0.0 2.5396520101091694E-4 0.0 8.568032452012005 0.0 76-77 0.0 2.5396520101091694E-4 0.0 9.140317635970005 0.0 78-79 0.0 2.5396520101091694E-4 0.0 9.781732147643178 0.0 80-81 0.0 2.5396520101091694E-4 0.0 10.535653243364186 0.0 82-83 0.0 2.624307077112809E-4 0.0 11.29367164432817 0.0 84-85 0.0 2.8782722781237253E-4 0.0 12.016439675391839 0.0 86-87 0.0 3.0475824121310035E-4 0.0 12.835206552437635 0.0 88 0.0 3.216892546138281E-4 0.0 13.493628267071838 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5750 0.0 46.307495 1 GTATCAA 9215 0.0 35.729866 1 TCAACGC 9665 0.0 33.053333 4 ATCAACG 9685 0.0 32.932217 3 CAACGCA 10420 0.0 30.75607 5 TATCAAC 10960 0.0 29.568254 2 AACGCAG 11445 0.0 27.867134 6 GTGGTAT 3570 0.0 23.809793 1 ACGCAGA 13305 0.0 23.702047 7 CGCAGAG 13240 0.0 23.607426 8 GTCTCGC 1965 0.0 23.452856 1 GTACATG 14040 0.0 23.414438 1 TCTGTCG 1925 0.0 23.027876 8 TACATGG 13995 0.0 22.021969 2 TACCTGG 3740 0.0 21.628082 2 TGGTATC 3540 0.0 20.969944 2 TCGCTCT 2170 0.0 20.52542 4 ACATGGG 14495 0.0 20.238064 3 CGCTCTG 1965 0.0 19.800459 5 GCAGAGT 14940 0.0 19.77066 9 >>END_MODULE