FastQCFastQC Report
Wed 25 May 2016
SRR2049514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049514_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5906321
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213350.36122317090452755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206490.3496084957116283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126290.21382176823779134No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66470.11254044607463766No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62950.10658072935758146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115250.056.8948331
GTATCAA212100.040.6137471
TATAACG5900.033.490992
GTGGTAT48150.033.3365551
ATCAACG270450.031.5904563
TCAACGC272750.031.410314
TGGTATC49700.031.3330232
TATCAAC273850.031.2328662
CAACGCA281500.030.4194895
ATAACGC5850.029.7558863
AACGCAG296100.029.125676
CGCAGAG335950.025.3208648
ACGCAGA338250.025.2876347
TACCTGG40250.024.0786442
GCAGAGT358250.023.4429879
TAACGCA9250.023.3960824
GTACATG138250.022.2676941
AGAGTAC340250.021.04354710-11
GAGTACT285000.020.66240312-13
TACATGG141400.020.4956652
Produced by FastQC (version 0.11.5)