##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049514_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5906321 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.725816629336602 31.0 31.0 34.0 30.0 34.0 2 31.76748825537928 31.0 31.0 34.0 30.0 34.0 3 31.788864506348368 33.0 31.0 34.0 30.0 34.0 4 35.51656013955218 37.0 35.0 37.0 33.0 37.0 5 35.65557933610449 37.0 35.0 37.0 33.0 37.0 6 35.74367072158794 37.0 35.0 37.0 33.0 37.0 7 35.75865246741584 37.0 35.0 37.0 33.0 37.0 8 35.758970770467776 37.0 35.0 37.0 33.0 37.0 9 37.491439087039126 39.0 37.0 39.0 35.0 39.0 10-11 37.45711704460357 39.0 37.0 39.0 34.5 39.0 12-13 37.447780352608675 39.0 37.0 39.0 34.0 39.0 14-15 38.70311188640103 40.0 38.0 41.0 34.0 41.0 16-17 38.71486912072676 40.0 38.0 41.0 34.5 41.0 18-19 38.79418583920515 40.0 38.0 41.0 35.0 41.0 20-21 38.81763317638848 40.0 38.0 41.0 35.0 41.0 22-23 38.77528625687632 40.0 38.0 41.0 35.0 41.0 24-25 38.72968274159159 40.0 38.0 41.0 34.0 41.0 26-27 38.628402773909514 40.0 38.0 41.0 34.0 41.0 28-29 38.525461958467886 40.0 38.0 41.0 34.0 41.0 30-31 38.38178927288239 40.0 38.0 41.0 34.0 41.0 32-33 38.19388820892058 40.0 38.0 41.0 34.0 41.0 34-35 38.06370547757225 40.0 37.5 41.0 33.5 41.0 36-37 37.89738061984779 40.0 37.0 41.0 33.0 41.0 38-39 37.776569035106625 40.0 37.0 41.0 33.0 41.0 40-41 37.63628949391677 40.0 37.0 41.0 33.0 41.0 42-43 37.4702807382125 40.0 37.0 41.0 33.0 41.0 44-45 37.26156189275862 40.0 37.0 41.0 32.0 41.0 46-47 36.979442194218706 40.0 37.0 41.0 31.0 41.0 48-49 36.707285211894174 40.0 36.5 41.0 31.0 41.0 50-51 36.477865916871096 40.0 36.0 41.0 30.0 41.0 52-53 36.36022347921828 40.0 36.0 41.0 30.0 41.0 54-55 36.21679697056763 39.0 35.0 41.0 30.0 41.0 56-57 36.042571086129584 39.0 35.0 41.0 29.5 41.0 58-59 35.86604334576465 39.0 35.0 41.0 29.0 41.0 60-61 35.6633707345063 39.0 35.0 41.0 29.0 41.0 62-63 35.437682611561414 38.5 35.0 40.5 28.5 41.0 64-65 35.13957038569356 38.0 35.0 40.0 28.0 41.0 66-67 34.87893402678249 37.0 34.5 40.0 28.0 41.0 68-69 34.55702221399751 37.0 34.0 40.0 28.0 41.0 70-71 34.24758881205407 36.0 34.0 39.0 27.5 41.0 72-73 33.92082998875273 36.0 34.0 39.0 27.0 41.0 74-75 33.58978804233634 35.0 34.0 39.0 26.0 40.5 76-77 33.307803876558694 35.0 34.0 37.5 26.0 40.0 78-79 32.98739232087115 35.0 33.5 37.0 26.0 39.0 80-81 32.68850660165609 35.0 33.0 36.5 26.0 39.0 82-83 32.40685665408297 35.0 33.0 36.0 26.0 38.5 84-85 32.13936111836793 35.0 33.0 36.0 25.0 37.0 86-87 31.867776404296347 35.0 33.0 35.5 25.0 37.0 88-89 31.66396941514015 35.0 33.0 35.0 25.0 36.5 90-91 31.513874372896428 35.0 33.0 35.0 25.0 36.0 92-93 31.35270128392954 35.0 33.0 35.0 24.0 36.0 94-95 31.15921256227015 35.0 33.0 35.0 23.5 36.0 96-97 30.980737840019195 35.0 33.0 35.0 21.5 35.5 98-99 30.79428861045649 35.0 32.5 35.0 19.5 35.0 100 30.6766176440461 35.0 32.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37336407371445546 1101 2 0.3768530374579484 1101 3 0.4900083480518269 1101 4 0.3602882722404672 1101 5 0.29476073134718916 1101 6 0.25306647389050596 1101 7 0.25856048216915894 1101 8 0.3128133933092059 1101 9 0.32922260176819407 1101 10-11 0.35137960426663284 1101 12-13 0.2837743320972166 1101 14-15 0.4425756251391775 1101 16-17 0.46515369892118485 1101 18-19 0.40713133416528535 1101 20-21 0.3787196579166192 1101 22-23 0.4276551584672248 1101 24-25 0.4622619268234942 1101 26-27 0.45340068257426225 1101 28-29 0.3992961648652553 1101 30-31 0.41515291538143373 1101 32-33 0.42580092759578747 1101 34-35 0.40195055464604934 1101 36-37 0.42184638376804173 1101 38-39 0.3960331372524095 1101 40-41 0.4645640356552221 1101 42-43 0.4129987731183178 1101 44-45 0.44635630834083173 1101 46-47 0.5466190416390617 1101 48-49 0.503950104170265 1101 50-51 0.5206246792228697 1101 52-53 0.5814419011180192 1101 54-55 0.4605003619612731 1101 56-57 0.45827682061629105 1101 58-59 0.4558165126298874 1101 60-61 0.4681038034851426 1101 62-63 0.4659534717839975 1101 64-65 0.5377317083124922 1101 66-67 0.4739882329848655 1101 68-69 0.4967759445428328 1101 70-71 0.4945170942079571 1101 72-73 0.4223030808666408 1101 74-75 0.45815500199567794 1101 76-77 0.4290404951816811 1101 78-79 0.4366136488735535 1101 80-81 0.46708204886012084 1101 82-83 0.4650402524729387 1101 84-85 0.4829146802202189 1101 86-87 0.599064174165548 1101 88-89 0.5865137620148175 1101 90-91 0.5559506471057674 1101 92-93 0.5373357453738414 1101 94-95 0.42903338324326157 1101 96-97 0.5079682883298275 1101 98-99 0.5989672949199729 1101 100 0.6515911815717885 1102 1 0.2033914130458001 1102 2 0.24272836499934414 1102 3 0.3656673606744114 1102 4 0.3151940068072179 1102 5 0.24913244850289118 1102 6 0.22378807108162846 1102 7 0.21144031460181623 1102 8 0.26256296256833167 1102 9 0.3316726620256816 1102 10-11 0.31689310066725795 1102 12-13 0.2654293501568503 1102 14-15 0.3660957720996336 1102 16-17 0.40221073372433835 1102 18-19 0.39343093431270404 1102 20-21 0.3497634356318713 1102 22-23 0.35560527662650543 1102 24-25 0.30637639711899567 1102 26-27 0.2777253341618007 1102 28-29 0.3379052538169347 1102 30-31 0.34151782346289394 1102 32-33 0.3957995009661559 1102 34-35 0.4345204870176431 1102 36-37 0.38704271926729916 1102 38-39 0.3991045079220541 1102 40-41 0.3791615389653131 1102 42-43 0.4121244781221307 1102 44-45 0.39376142756347576 1102 46-47 0.45440000731761643 1102 48-49 0.4929822284349896 1102 50-51 0.4799971074918048 1102 52-53 0.4404233712246324 1102 54-55 0.4486741087177819 1102 56-57 0.47961015130772466 1102 58-59 0.4482467673933499 1102 60-61 0.4413015277466954 1102 62-63 0.3994249328068449 1102 64-65 0.3738822608893031 1102 66-67 0.4406936916484909 1102 68-69 0.4336449636513038 1102 70-71 0.4309369451427827 1102 72-73 0.47859575041209723 1102 74-75 0.47899980010337373 1102 76-77 0.41018861940590057 1102 78-79 0.42149236554094927 1102 80-81 0.42224396031485156 1102 82-83 0.4377910101497733 1102 84-85 0.4499402278984945 1102 86-87 0.49948996146476077 1102 88-89 0.4337817882947448 1102 90-91 0.4187045618611265 1102 92-93 0.48569582581109216 1102 94-95 0.5226083541712789 1102 96-97 0.566761192344515 1102 98-99 0.5867343641691924 1102 100 0.6367460875705468 1103 1 0.3018192970481053 1103 2 0.3195836195870996 1103 3 0.4382490836530657 1103 4 0.34921177026768646 1103 5 0.2608792311435906 1103 6 0.23140316128392868 1103 7 0.2751829085178841 1103 8 0.25758935686820905 1103 9 0.28400257126682504 1103 10-11 0.2908087571516802 1103 12-13 0.2990863945078317 1103 14-15 0.39025373634365934 1103 16-17 0.38981012828218553 1103 18-19 0.4100630279462649 1103 20-21 0.3872570424554098 1103 22-23 0.3518224845433906 1103 24-25 0.3873908576965803 1103 26-27 0.3724472832841954 1103 28-29 0.4651374186426054 1103 30-31 0.48535952462246 1103 32-33 0.45178813784824 1103 34-35 0.42093712363261915 1103 36-37 0.4756758354419688 1103 38-39 0.43303936359847484 1103 40-41 0.4712636218802331 1103 42-43 0.38708149111035084 1103 44-45 0.41614315448736505 1103 46-47 0.49919821846938817 1103 48-49 0.4847181516597985 1103 50-51 0.5307607606910381 1103 52-53 0.4393864696992651 1103 54-55 0.4291888520346987 1103 56-57 0.5001739363827795 1103 58-59 0.4863751698125327 1103 60-61 0.43817947744899044 1103 62-63 0.44259644847396373 1103 64-65 0.42020509133209316 1103 66-67 0.43632475572312046 1103 68-69 0.40318376267977385 1103 70-71 0.3995231059859705 1103 72-73 0.4230748015432084 1103 74-75 0.44388111333556424 1103 76-77 0.3974479670838136 1103 78-79 0.35362798462981004 1103 80-81 0.3202645477374375 1103 82-83 0.3315488186291091 1103 84-85 0.4051971779931449 1103 86-87 0.4536235086144629 1103 88-89 0.4743524579000322 1103 90-91 0.4325604202502582 1103 92-93 0.4266592564816811 1103 94-95 0.4483357444814864 1103 96-97 0.48106707698130435 1103 98-99 0.4279790684812603 1103 100 0.4431554586875741 1104 1 0.24370404164478643 1104 2 0.25046028696880995 1104 3 0.32326371383731 1104 4 0.26991652885667605 1104 5 0.20687218080803405 1104 6 0.19233122520345347 1104 7 0.14586059838493526 1104 8 0.20554622941553902 1104 9 0.23032297395290158 1104 10-11 0.20308459271861068 1104 12-13 0.22736775340148085 1104 14-15 0.34391578431065994 1104 16-17 0.33593131487952377 1104 18-19 0.3276882872523572 1104 20-21 0.30827524289389174 1104 22-23 0.3336285023923722 1104 24-25 0.33340216543361123 1104 26-27 0.36520320401533723 1104 28-29 0.3692639480860507 1104 30-31 0.37159847785108724 1104 32-33 0.38609705716903164 1104 34-35 0.41594572948459785 1104 36-37 0.4372567431543075 1104 38-39 0.4811200048694033 1104 40-41 0.48735262591368667 1104 42-43 0.47476525295708427 1104 44-45 0.5434104590094364 1104 46-47 0.5372666136119335 1104 48-49 0.5402579730082593 1104 50-51 0.5915832387356517 1104 52-53 0.5715437002559653 1104 54-55 0.5384921827241769 1104 56-57 0.5021335662802642 1104 58-59 0.5326388201750873 1104 60-61 0.5034760617489695 1104 62-63 0.4966932808370643 1104 64-65 0.49246860461565234 1104 66-67 0.4958176752962302 1104 68-69 0.45020651664810174 1104 70-71 0.43875632218212957 1104 72-73 0.4564403625942788 1104 74-75 0.48168134178708044 1104 76-77 0.46414428028547405 1104 78-79 0.44445380068960816 1104 80-81 0.4522682147691768 1104 82-83 0.44148522650828426 1104 84-85 0.43878725476749736 1104 86-87 0.4892211390213106 1104 88-89 0.4427666122435667 1104 90-91 0.49739407650100986 1104 92-93 0.4622228395380432 1104 94-95 0.4252835855175583 1104 96-97 0.43054945548930945 1104 98-99 0.46565529233086167 1104 100 0.4985114634318286 1105 1 0.19145452163301613 1105 2 0.1697934528452798 1105 3 0.275302752557554 1105 4 0.23989681110077754 1105 5 0.13049421480564405 1105 6 0.18339730472689553 1105 7 0.15649391599743012 1105 8 0.21144276079600388 1105 9 0.22669047486801475 1105 10-11 0.1913265296028399 1105 12-13 0.18173844527871807 1105 14-15 0.2404323914489197 1105 16-17 0.22512961223380756 1105 18-19 0.23825365527749653 1105 20-21 0.24697152738183803 1105 22-23 0.2764446511899834 1105 24-25 0.24986907307573603 1105 26-27 0.2562787392440242 1105 28-29 0.3112096305704384 1105 30-31 0.2592880971303728 1105 32-33 0.278104163063837 1105 34-35 0.20011923800179687 1105 36-37 0.25987736189002675 1105 38-39 0.2453759694567239 1105 40-41 0.234143574193304 1105 42-43 0.24988733113933392 1105 44-45 0.2468798326762851 1105 46-47 0.2819027052847076 1105 48-49 0.24036384210068462 1105 50-51 0.2846766872081474 1105 52-53 0.2944867991477551 1105 54-55 0.3100229492492801 1105 56-57 0.2926287455142216 1105 58-59 0.2905972077240335 1105 60-61 0.26921591460736494 1105 62-63 0.2537735833654864 1105 64-65 0.34344059890510437 1105 66-67 0.33395531903890685 1105 68-69 0.31624819793823633 1105 70-71 0.3149075059532791 1105 72-73 0.23919012035226217 1105 74-75 0.3119515873898422 1105 76-77 0.22179590851343534 1105 78-79 0.19503596467706075 1105 80-81 0.19317394370833085 1105 82-83 0.2069383723285938 1105 84-85 0.2053954855625051 1105 86-87 0.21363631138682138 1105 88-89 0.1799489977149662 1105 90-91 0.17697495478326175 1105 92-93 0.192837164525411 1105 94-95 0.1990100866881157 1105 96-97 0.22218844633158596 1105 98-99 0.26042816300369154 1105 100 0.27351599308203944 1106 1 0.14201392600529417 1106 2 0.1271097419315197 1106 3 0.24358247248921927 1106 4 0.24644755466370327 1106 5 0.16576726310314172 1106 6 0.15272746284076533 1106 7 0.1826367988618074 1106 8 0.17490518555464263 1106 9 0.1273538234320739 1106 10-11 0.19548828349745406 1106 12-13 0.1607647753029795 1106 14-15 0.223208578028121 1106 16-17 0.23433067904654337 1106 18-19 0.22559709153897245 1106 20-21 0.21047381437509927 1106 22-23 0.20405437363619683 1106 24-25 0.19696335646246865 1106 26-27 0.22535100360162374 1106 28-29 0.22149015303691755 1106 30-31 0.22849394964652703 1106 32-33 0.26475507061204695 1106 34-35 0.24612779457326184 1106 36-37 0.2865506422105568 1106 38-39 0.26901812421440496 1106 40-41 0.251299106462362 1106 42-43 0.18292791671828468 1106 44-45 0.21944092425179917 1106 46-47 0.21120114597467676 1106 48-49 0.24182913314026422 1106 50-51 0.31597332858390104 1106 52-53 0.2713047365373953 1106 54-55 0.2304100507888336 1106 56-57 0.2917802263148985 1106 58-59 0.2724462806855428 1106 60-61 0.29684981890076756 1106 62-63 0.28586727695258674 1106 64-65 0.2934865690026953 1106 66-67 0.32020987055170025 1106 68-69 0.28955710379354116 1106 70-71 0.28075551160035417 1106 72-73 0.31203311367725206 1106 74-75 0.31953656346014014 1106 76-77 0.305378154182101 1106 78-79 0.31959946855108967 1106 80-81 0.2930983768328943 1106 82-83 0.29412935479074065 1106 84-85 0.2773225229700529 1106 86-87 0.3127516963752299 1106 88-89 0.30304599978061475 1106 90-91 0.3181854990720261 1106 92-93 0.2931623248712505 1106 94-95 0.2937024773364776 1106 96-97 0.33295856769172616 1106 98-99 0.3598617380350433 1106 100 0.3903324827647303 1107 1 0.11326549174087575 1107 2 0.18317622815613888 1107 3 0.2124896861581611 1107 4 0.1609181978703944 1107 5 0.1488261104125712 1107 6 0.143338701662735 1107 7 0.1405188016274721 1107 8 0.14008369245451036 1107 9 0.12838246967805844 1107 10-11 0.14604448799401837 1107 12-13 0.1127970454949292 1107 14-15 0.17501904977009275 1107 16-17 0.17719582346331464 1107 18-19 0.13411892990288266 1107 20-21 0.16679824599520288 1107 22-23 0.17189633395840076 1107 24-25 0.16306407606314366 1107 26-27 0.14452899870257596 1107 28-29 0.1703345015285933 1107 30-31 0.19795240755592403 1107 32-33 0.1845000409824067 1107 34-35 0.13591745580045256 1107 36-37 0.1759591113182708 1107 38-39 0.20180254145931542 1107 40-41 0.14200204903072944 1107 42-43 0.12010191303395601 1107 44-45 0.1541755724100966 1107 46-47 0.24883418760420994 1107 48-49 0.18287014246529765 1107 50-51 0.2565293995025968 1107 52-53 0.1516819041705233 1107 54-55 0.10923426694517957 1107 56-57 0.10700361465558217 1107 58-59 0.10467477242564627 1107 60-61 0.14957441620575196 1107 62-63 0.16748752800555877 1107 64-65 0.11113868328196475 1107 66-67 0.11245150582313812 1107 68-69 0.15938746496685496 1107 70-71 0.21328981454318097 1107 72-73 0.18450684932040673 1107 74-75 0.17558566161383737 1107 76-77 0.184057179885869 1107 78-79 0.0887375666224628 1107 80-81 0.11247641144197473 1107 82-83 0.1896373567575509 1107 84-85 0.17955952842757483 1107 86-87 0.19242896909284468 1107 88-89 0.14468857331462814 1107 90-91 0.13389804692817364 1107 92-93 0.1313415659421615 1107 94-95 0.15152304635792646 1107 96-97 0.20519796837201199 1107 98-99 0.3007318498095266 1107 100 0.25497564433124964 1108 1 0.09157943928358492 1108 2 0.09032622474729379 1108 3 0.1471107318951539 1108 4 0.11419020441537953 1108 5 0.08520971288252355 1108 6 0.12964365715292558 1108 7 0.10454937411905263 1108 8 0.12115089301160964 1108 9 0.10600521253408601 1108 10-11 0.10157074707615976 1108 12-13 0.13855545317360196 1108 14-15 0.136809370347855 1108 16-17 0.14954154876864578 1108 18-19 0.14394554131375514 1108 20-21 0.15079614224936932 1108 22-23 0.1370682616129173 1108 24-25 0.16769691275987242 1108 26-27 0.1395642559244621 1108 28-29 0.17794871009161994 1108 30-31 0.18825636717568983 1108 32-33 0.15898223097207875 1108 34-35 0.1243333096193382 1108 36-37 0.13745785369706454 1108 38-39 0.18932783388972751 1108 40-41 0.1452263012756987 1108 42-43 0.1614306878454954 1108 44-45 0.2542088086724448 1108 46-47 0.18445749535494826 1108 48-49 0.1496871433613407 1108 50-51 0.24137676835935906 1108 52-53 0.15850340352771752 1108 54-55 0.15338675442983885 1108 56-57 0.14271874823370467 1108 58-59 0.14497511014503317 1108 60-61 0.17443039240225744 1108 62-63 0.1456317940807068 1108 64-65 0.11301187537539192 1108 66-67 0.1356045801165351 1108 68-69 0.15784998347201906 1108 70-71 0.2355138257339391 1108 72-73 0.17982516262270565 1108 74-75 0.2805573133952066 1108 76-77 0.20783860824163725 1108 78-79 0.18387149780270562 1108 80-81 0.19066182717526914 1108 82-83 0.22221848123891874 1108 84-85 0.24466197037976656 1108 86-87 0.2872468209832917 1108 88-89 0.2473158815913088 1108 90-91 0.19975789216649886 1108 92-93 0.20840175766548086 1108 94-95 0.16488116282362952 1108 96-97 0.2113182191170857 1108 98-99 0.27624323779480164 1108 100 0.27173225993013617 1109 1 0.011629644852956034 1109 2 0.013656780062795804 1109 3 0.1198196662609341 1109 4 0.012147769403831887 1109 5 0.045432956605047536 1109 6 0.07346899954165309 1109 7 0.040291335723210864 1109 8 0.07863952543367958 1109 9 0.04990069592695079 1109 10-11 0.07108427038014042 1109 12-13 0.0628879594152636 1109 14-15 0.09940142671635499 1109 16-17 0.06614078733921502 1109 18-19 0.11133974085987575 1109 20-21 0.1075025692962086 1109 22-23 0.059572366489405226 1109 24-25 0.11868196473523795 1109 26-27 0.19055824569867497 1109 28-29 0.09784664753176031 1109 30-31 0.1512259123660371 1109 32-33 0.12859371491896354 1109 34-35 0.13725671564282038 1109 36-37 0.1169583313661775 1109 38-39 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-0.6999752544499529 2215 22-23 -0.6903608781830286 2215 24-25 -0.705917888587301 2215 26-27 -0.6225124935064414 2215 28-29 -0.583567237006811 2215 30-31 -0.6084181594509701 2215 32-33 -0.7254873682628684 2215 34-35 -0.8085169559889849 2215 36-37 -0.8515893558088052 2215 38-39 -0.8854517893744145 2215 40-41 -0.8494011236981933 2215 42-43 -0.7959418843934856 2215 44-45 -0.983258325481529 2215 46-47 -0.9781528207559802 2215 48-49 -1.047194783001423 2215 50-51 -0.9843175602483427 2215 52-53 -1.093893910313568 2215 54-55 -1.0215911428637767 2215 56-57 -0.9983521764541763 2215 58-59 -0.9190376361994268 2215 60-61 -1.075500161857093 2215 62-63 -1.0359160630082798 2215 64-65 -0.9221946658432856 2215 66-67 -0.806728502432307 2215 68-69 -0.8384968411832432 2215 70-71 -0.9491299093818881 2215 72-73 -0.8223921813887358 2215 74-75 -0.9618700075937738 2215 76-77 -0.9367158469739465 2215 78-79 -0.9484005945364373 2215 80-81 -0.9361140433584438 2215 82-83 -0.9387667855378794 2215 84-85 -0.9012074670670778 2215 86-87 -0.8521071110814162 2215 88-89 -0.775223864218276 2215 90-91 -0.8194285154038603 2215 92-93 -0.9206798845968542 2215 94-95 -1.0266477616968501 2215 96-97 -1.0590673257298278 2215 98-99 -1.0192897414785698 2215 100 -1.1109608986783606 2216 1 -0.6222355664114971 2216 2 -0.9046099790576996 2216 3 -0.6515415523401806 2216 4 -1.1515267782061613 2216 5 -0.6270263003345775 2216 6 -0.8691186041893104 2216 7 -0.5582174883982916 2216 8 -0.5432801739422572 2216 9 -0.5781152299906935 2216 10-11 -0.8226408957012339 2216 12-13 -0.6572114657306898 2216 14-15 -0.9419421436417537 2216 16-17 -0.8578952696541009 2216 18-19 -0.9049290090003197 2216 20-21 -0.6892841978767095 2216 22-23 -0.699984324856473 2216 24-25 -0.7231634957071904 2216 26-27 -0.894321571779578 2216 28-29 -1.1106245985818148 2216 30-31 -1.2564171636623271 2216 32-33 -0.9710903390323224 2216 34-35 -0.9994312974200099 2216 36-37 -1.0048002970365317 2216 38-39 -1.2218306247166097 2216 40-41 -0.9502731856030593 2216 42-43 -1.5379759071721537 2216 44-45 -1.2608405299086343 2216 46-47 -1.133320486668886 2216 48-49 -1.133587393419866 2216 50-51 -1.3119218794815808 2216 52-53 -1.2283719925906027 2216 54-55 -1.383106090066235 2216 56-57 -1.4174255121386707 2216 58-59 -1.246933182119811 2216 60-61 -1.4533382632200826 2216 62-63 -1.4841466503161271 2216 64-65 -1.435157116313178 2216 66-67 -1.2658234757742122 2216 68-69 -1.573878062269074 2216 70-71 -1.33687863871409 2216 72-73 -1.1915493707752915 2216 74-75 -1.303450614609936 2216 76-77 -1.4477805317446055 2216 78-79 -1.4852623873709092 2216 80-81 -1.2283463940420098 2216 82-83 -1.2912463614978797 2216 84-85 -1.3048812840494257 2216 86-87 -1.5134354723867318 2216 88-89 -1.3174558414811024 2216 90-91 -1.229136043980919 2216 92-93 -1.340688867440246 2216 94-95 -1.4023536098805742 2216 96-97 -1.5131091488528696 2216 98-99 -1.5266109455530064 2216 100 -1.5081837686242139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 341.0 3 24.0 4 44.0 5 140.0 6 457.0 7 1207.0 8 2673.0 9 4513.0 10 6752.0 11 9911.0 12 14904.0 13 20393.0 14 22281.0 15 26769.0 16 33586.0 17 46323.0 18 46399.0 19 25356.0 20 18673.0 21 17838.0 22 18262.0 23 19349.0 24 22948.0 25 27753.0 26 34500.0 27 44329.0 28 57287.0 29 74463.0 30 97047.0 31 124107.0 32 160072.0 33 208570.0 34 282500.0 35 409412.0 36 660748.0 37 1209291.0 38 1564920.0 39 590304.0 40 1875.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50857297165066 20.49317160817462 14.039191889211558 22.95906353096316 2 16.683479194285432 21.062186034935518 38.44943797396344 23.80489679681561 3 18.182760161995965 25.21167156537404 30.852154979750505 25.75341329287949 4 13.181129763177053 17.941726113240744 37.43275552139102 31.444388602191182 5 12.291484902290414 36.575278938652374 34.65191906779065 16.481317091266558 6 29.67241762016888 37.97129427692112 17.669083501786 14.687204601124005 7 25.83379738419229 32.67995423885698 22.64876900527418 18.837479371676547 8 24.715148397792806 34.524689734946676 21.30255026775551 19.457611599505004 9 24.208267182408257 16.595942953959295 22.749507010883434 36.446282852749015 10-11 23.267758389698088 26.564861611822316 28.818565736606594 21.348814261873 12-13 24.725941826034116 24.25488701169036 29.01632979864447 22.002841363631052 14-15 22.087359141340052 26.490117915226225 27.278443030384658 24.144079913049065 16-17 20.417371490645362 29.402584112851297 27.751598668612832 22.42844572789051 18-19 20.064842395122106 27.14500278599826 31.430809466671384 21.359345352208255 20-21 21.603439772406546 26.844096350333817 30.944449175722077 20.608014701537556 22-23 21.11778211851337 27.065934953416853 31.0077880968542 20.808494831215572 24-25 20.842644685244842 26.790670537547822 31.32717473364553 21.039510043561805 26-27 20.68908039370024 26.479673217896554 31.444235760298163 21.387010628105042 28-29 20.830540768400432 26.54730954199226 31.430266468471807 21.1918832211355 30-31 21.426587471065876 27.3138305009503 30.687555983610395 20.57202604437343 32-33 20.555333853341192 26.95479978145448 31.41373453965675 21.076131825547577 34-35 20.59804233464453 26.237254968024935 31.464730752019744 21.699971945310796 36-37 21.599063105442458 27.00571980425717 30.450867807557362 20.944349282743012 38-39 21.376234038075477 26.96876786751008 30.71996933454853 20.93502875986591 40-41 20.89777206487761 26.715276734874383 30.957934727895758 21.429016472352245 42-43 20.900904302356746 26.746150437810613 30.66601866034711 21.686926599485535 44-45 21.35560359824669 27.040191347541047 30.32032122873105 21.283883825481208 46-47 21.153599677362607 27.68968195260637 29.68384210746419 21.472876262566835 48-49 21.098015160368018 27.076694612433016 29.962882139321582 21.862408087877377 50-51 21.196773761534466 26.849158723340636 29.713615294529372 22.24045222059553 52-53 21.107174838617812 27.13190664713279 29.531158228616427 22.229760285632967 54-55 21.331214473442945 27.743420989140276 29.197278644354075 21.728085893062705 56-57 20.755610810858403 27.406400701892093 29.23245282469409 22.605535662555422 58-59 20.512223151077766 26.767403519539474 29.79331858922128 22.92705474016148 60-61 21.198585379968343 27.294046496964864 29.063819931222838 22.44354819184396 62-63 20.96167817495866 27.226677994643367 29.215191656532046 22.59645217386593 64-65 20.75539917319089 26.842530231594253 29.62741103979956 22.77465955541529 66-67 20.688504739244614 27.003603427582078 29.115764280336272 23.192127552837036 68-69 21.39922635427367 27.25254858311968 28.727510746537483 22.620714316069172 70-71 21.388559815831208 26.87919434111353 28.79261895856998 22.939626884485286 72-73 20.94316411180496 27.038684487348384 29.178315909345258 22.839835491501393 74-75 20.929102905175657 27.008902834776507 28.894255832014547 23.16773842803329 76-77 20.977415551914635 27.31078280371148 28.42842439481362 23.283377249560257 78-79 21.18451003844952 27.499132073117327 28.381873606311753 22.9344842821214 80-81 20.89353033420912 27.19279339254918 28.504282374995388 23.409393898246314 82-83 20.68531731495883 27.193714576493356 28.62046216578381 23.500505942764 84-85 20.881653740120118 27.171042684608576 28.270339522691028 23.676964052580278 86-87 21.060948272359102 27.380678662856063 28.108864741497243 23.449508323287596 88-89 20.70975872104393 27.224393814283925 28.208585824296257 23.85726164037589 90-91 20.468222453029735 27.067919573900245 28.103040194341094 24.360817778728926 92-93 20.799803666422886 27.247472158634007 27.782352479553733 24.17037169538937 94-95 20.715788956839162 27.006982398396286 28.0129624738414 24.26426617092315 96-97 20.51061904694987 27.041875983374418 28.08004339757355 24.367461572102158 98-99 20.5062341287555 27.054323264145 28.016707869716136 24.422734737383365 100 20.74056666307161 27.119475417696577 27.681472254612522 24.458485664619293 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1104.0 1 950.5 2 2846.5 3 4502.0 4 2652.5 5 1247.0 6 2211.5 7 4921.0 8 7712.0 9 8687.5 10 6951.5 11 4746.5 12 4319.0 13 4685.5 14 5333.5 15 6013.0 16 6749.5 17 7535.0 18 8344.0 19 9398.5 20 11064.0 21 13257.0 22 16291.5 23 21103.5 24 26909.5 25 33508.5 26 42946.0 27 55466.0 28 71650.0 29 87993.5 30 101439.5 31 114393.5 32 126754.0 33 138648.0 34 150709.5 35 161764.0 36 170416.0 37 177352.5 38 183304.0 39 187924.0 40 191105.5 41 191903.0 42 190045.5 43 189173.0 44 189555.5 45 189018.0 46 188623.0 47 187794.0 48 186661.5 49 188542.5 50 189062.0 51 183731.5 52 175867.0 53 170056.5 54 166679.0 55 161776.5 56 153250.5 57 140587.5 58 126665.0 59 112843.5 60 96510.0 61 79219.0 62 64216.0 63 50680.5 64 39034.0 65 29850.5 66 22137.0 67 16150.5 68 11834.0 69 8644.0 70 6233.5 71 4533.0 72 3420.5 73 2514.5 74 1803.0 75 1194.5 76 771.5 77 515.0 78 341.0 79 241.0 80 140.5 81 66.0 82 28.0 83 14.0 84 10.5 85 7.0 86 4.0 87 2.0 88 0.5 89 0.0 90 0.5 91 1.5 92 1.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.5 98 1.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05869982346032327 2 1.8624114740800577E-4 3 1.6931013400727794E-5 4 0.11455523666932428 5 0.0015915152596684128 6 0.0018962735008815133 7 0.0 8 0.0 9 6.772405360291118E-5 10-11 0.0 12-13 3.386202680145559E-5 14-15 2.5396520101091693E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.1163766750909745E-4 30-31 2.7936172111200864E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.4391361390618627E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 9.312057370400289E-5 80-81 3.216892546138281E-4 82-83 3.4708577471491983E-4 84-85 0.0 86-87 1.6931013400727794E-5 88-89 6.772405360291118E-5 90-91 7.618956030327509E-5 92-93 7.872921231338426E-4 94-95 6.941715494298397E-4 96-97 0.0 98-99 4.2327533501819494E-5 100 0.001015860804043668 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5906321.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.74983910324432 #Duplication Level Percentage of deduplicated Percentage of total 1 79.27606737538522 50.53836539918765 2 13.010006127292744 16.587715946942705 3 3.563502665045503 6.815181646218995 4 1.4215697119960424 3.6249936165517234 5 0.750232540266226 2.3913601865995093 6 0.4348853361323975 1.6634322124080398 7 0.3019978658848083 1.3476620751775783 8 0.21720216055023236 1.1077282230363483 9 0.1555563651829769 0.8925023926710265 >10 0.807005664957839 9.076238540064576 >50 0.04218030944299414 1.8216848374595018 >100 0.01838498387647396 2.1009837255943227 >500 8.772401866479711E-4 0.39985487549324916 >1k 3.9874034988167563E-4 0.48748077804189177 >5k 5.316537996730726E-5 0.21932059473916882 >10k+ 7.974806995096089E-5 0.9254949498138021 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21335 0.36122317090452755 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20649 0.3496084957116283 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12629 0.21382176823779134 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6647 0.11254044607463766 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6295 0.10658072935758146 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.6931013400727797E-5 0.0 2 0.0 0.0 0.0 1.6931013400727797E-5 0.0 3 0.0 0.0 0.0 1.6931013400727797E-5 0.0 4 0.0 0.0 0.0 1.6931013400727797E-5 0.0 5 0.0 5.079304020218339E-5 0.0 3.3862026801455595E-5 0.0 6 0.0 5.079304020218339E-5 0.0 3.3862026801455595E-5 0.0 7 0.0 5.079304020218339E-5 0.0 3.3862026801455595E-5 0.0 8 0.0 5.079304020218339E-5 0.0 3.3862026801455595E-5 0.0 9 0.0 5.079304020218339E-5 0.0 6.772405360291119E-5 0.0 10-11 0.0 5.079304020218339E-5 0.0 1.1851709380509458E-4 0.0 12-13 0.0 5.079304020218339E-5 0.0 1.7777564070764186E-4 0.0 14-15 0.0 6.772405360291119E-5 0.0 2.3703418761018916E-4 0.0 16-17 0.0 8.465506700363899E-5 0.0 2.3703418761018916E-4 0.0 18-19 0.0 8.465506700363899E-5 0.0 2.624307077112809E-4 0.0 20-21 0.0 8.465506700363899E-5 0.0 4.317408417185588E-4 0.0 22-23 0.0 8.465506700363899E-5 0.0 0.0011259123911483986 0.0 24-25 0.0 9.312057370400289E-5 0.0 0.002658169103914264 0.0 26-27 0.0 1.0158608040436678E-4 0.0 0.005011579966615428 0.0 28-29 0.0 1.0158608040436678E-4 0.0 0.013959620548900068 0.0 30-31 0.0 1.0158608040436678E-4 0.0 0.03943233021029503 0.0 32-33 0.0 1.0158608040436678E-4 0.0 0.0995882208230809 0.0 34-35 0.0 1.0158608040436678E-4 0.0 0.1975849263864934 0.0 36-37 0.0 1.0158608040436678E-4 0.0 0.3449186049996267 0.0 38-39 0.0 1.1005158710473068E-4 0.0 0.5466008366290962 0.0 40-41 0.0 1.1851709380509458E-4 0.0 0.789069879540919 0.0 42-43 0.0 1.1851709380509458E-4 0.0 1.0448805610125151 0.0 44-45 0.0 1.3544810720582238E-4 0.0 1.3431457585864366 0.0 46-47 0.0 1.5237912060655018E-4 0.0 1.6985107988543122 0.0 48-49 0.0 1.5237912060655018E-4 0.0 2.0802035649603194 0.0 50-51 0.0 1.5237912060655018E-4 0.0 2.475542727867314 0.0 52-53 0.0 1.6931013400727797E-4 0.0 2.863330658797583 0.0 54-55 0.0 1.6931013400727797E-4 0.0 3.301632268208924 0.0 56-57 8.465506700363899E-6 1.6931013400727797E-4 0.0 3.8062018640707134 0.0 58-59 1.6931013400727797E-5 1.8624114740800577E-4 0.0 4.341035646386304 0.0 60-61 1.6931013400727797E-5 1.8624114740800577E-4 0.0 4.885926450661927 0.0 62-63 1.6931013400727797E-5 1.8624114740800577E-4 0.0 5.419024804103943 0.0 64-65 1.6931013400727797E-5 1.8624114740800577E-4 0.0 6.0102642575640575 0.0 66-67 1.6931013400727797E-5 1.8624114740800577E-4 0.0 6.6805038195519675 0.0 68-69 1.6931013400727797E-5 1.8624114740800577E-4 0.0 7.371382286875367 0.0 70-71 1.6931013400727797E-5 1.8624114740800577E-4 0.0 8.09078951177899 0.0 72-73 1.6931013400727797E-5 1.9470665410836968E-4 0.0 8.865848977730806 0.0 74-75 1.6931013400727797E-5 2.0317216080873357E-4 0.0 9.617247352455106 0.0 76-77 1.6931013400727797E-5 2.0317216080873357E-4 0.0 10.37896517984715 0.0 78-79 1.6931013400727797E-5 2.2010317420946137E-4 0.0 11.202608188752356 0.0 80-81 1.6931013400727797E-5 2.3703418761018916E-4 0.0 12.06996707425824 0.0 82-83 1.6931013400727797E-5 2.3703418761018916E-4 0.0 12.943522710668791 0.0 84-85 1.6931013400727797E-5 2.5396520101091694E-4 0.0 13.778314791898374 0.0 86-87 1.6931013400727797E-5 2.624307077112809E-4 0.0 14.633525675289237 0.0 88 1.6931013400727797E-5 2.7089621441164476E-4 0.0 15.301792774215963 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11525 0.0 56.894833 1 GTATCAA 21210 0.0 40.613747 1 TATAACG 590 0.0 33.49099 2 GTGGTAT 4815 0.0 33.336555 1 ATCAACG 27045 0.0 31.590456 3 TCAACGC 27275 0.0 31.41031 4 TGGTATC 4970 0.0 31.333023 2 TATCAAC 27385 0.0 31.232866 2 CAACGCA 28150 0.0 30.419489 5 ATAACGC 585 0.0 29.755886 3 AACGCAG 29610 0.0 29.12567 6 CGCAGAG 33595 0.0 25.320864 8 ACGCAGA 33825 0.0 25.287634 7 TACCTGG 4025 0.0 24.078644 2 GCAGAGT 35825 0.0 23.442987 9 TAACGCA 925 0.0 23.396082 4 GTACATG 13825 0.0 22.267694 1 AGAGTAC 34025 0.0 21.043547 10-11 GAGTACT 28500 0.0 20.662403 12-13 TACATGG 14140 0.0 20.495665 2 >>END_MODULE