FastQCFastQC Report
Wed 25 May 2016
SRR2049513_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049513_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7432331
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66300.041.851491
GTATCAA100850.034.315951
ATCAACG102350.031.9057353
TCAACGC103500.031.5509874
CAACGCA110750.029.393145
TATCAAC121100.027.5152052
AACGCAG120200.027.1674486
GTACATG180600.024.6619111
ACGCAGA143000.022.5130337
TACATGG187900.022.4458642
CGCAGAG147350.021.6738878
ACATGGG191700.021.3067253
GTGGTAT43300.018.3260561
CATGGGG138050.017.9078454
GCAGAGT173900.017.3243459
GAGTACA137550.017.0090791
GTACCGT5251.8189894E-1216.5738056
AGAGTAC148300.016.16653310-11
CAGAGTA165600.015.7888210-11
TCTGTCG21000.015.7248238
Produced by FastQC (version 0.11.5)