##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049513_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7432331 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.86040247669271 31.0 31.0 34.0 28.0 34.0 2 30.98423603577397 31.0 31.0 34.0 28.0 34.0 3 31.178439846126338 31.0 31.0 34.0 28.0 34.0 4 34.76989762700289 37.0 35.0 37.0 32.0 37.0 5 34.76838706456965 37.0 35.0 37.0 32.0 37.0 6 34.72377750667994 37.0 35.0 37.0 32.0 37.0 7 30.831368382274686 37.0 33.0 37.0 2.0 37.0 8 32.60972634291987 37.0 33.0 37.0 17.0 37.0 9 35.407782296025296 39.0 34.0 39.0 29.0 39.0 10-11 35.95498713660626 39.0 36.0 39.0 31.0 39.0 12-13 36.10970044256641 39.0 37.0 39.0 31.5 39.0 14-15 37.31209428105395 40.0 38.0 41.0 31.0 41.0 16-17 37.32725466613368 40.0 38.0 41.0 31.0 41.0 18-19 37.3222134482439 40.0 37.5 41.0 31.0 41.0 20-21 37.38920158426744 40.0 38.0 41.0 31.0 41.0 22-23 37.34901836314879 40.0 37.5 41.0 31.0 41.0 24-25 37.32612500708055 40.0 37.0 41.0 31.0 41.0 26-27 37.35048755229012 40.0 37.0 41.0 31.0 41.0 28-29 37.33169923406264 40.0 37.0 41.0 31.0 41.0 30-31 37.25344350244896 40.0 37.0 41.0 31.0 41.0 32-33 37.20677759104109 40.0 37.0 41.0 31.0 41.0 34-35 37.15030197121199 40.0 37.0 41.0 31.0 41.0 36-37 36.98175861650941 40.0 37.0 41.0 30.0 41.0 38-39 36.863195274806785 40.0 37.0 41.0 30.0 41.0 40-41 36.78910492549377 40.0 37.0 41.0 30.0 41.0 42-43 36.67361814214141 40.0 36.5 41.0 30.0 41.0 44-45 36.55137769025626 40.0 36.0 41.0 30.0 41.0 46-47 36.39816694116556 40.0 36.0 41.0 30.0 41.0 48-49 36.31734377814982 40.0 36.0 41.0 30.0 41.0 50-51 36.18259823196787 39.5 35.0 41.0 29.0 41.0 52-53 36.058140709825764 39.0 35.0 41.0 29.0 41.0 54-55 35.934081514937915 39.0 35.0 41.0 29.0 41.0 56-57 35.73018969418881 39.0 35.0 41.0 28.0 41.0 58-59 35.549232266431616 39.0 35.0 41.0 28.0 41.0 60-61 35.32475787205925 38.5 35.0 40.0 28.0 41.0 62-63 35.080815291999244 38.0 34.0 40.0 28.0 41.0 64-65 34.7191907895383 37.5 34.0 40.0 27.0 41.0 66-67 31.22854370990743 36.0 31.0 40.0 6.5 41.0 68-69 31.57730650047744 36.0 30.5 39.0 11.0 41.0 70-71 31.732388264193293 35.5 30.0 39.0 15.5 41.0 72-73 32.96798514490273 35.0 32.0 39.0 24.5 40.5 74-75 33.0538976264647 35.0 33.0 38.5 25.5 40.0 76-77 32.75956715329282 35.0 33.0 37.5 25.0 39.5 78-79 29.38474618528158 35.0 29.0 36.5 6.5 39.0 80-81 31.168564276806293 35.0 30.0 36.0 21.0 39.0 82-83 31.585805314644894 35.0 32.0 36.0 23.0 37.5 84-85 29.761832055111647 35.0 30.5 35.5 12.0 37.0 86-87 29.397482162729297 34.5 29.0 35.0 13.5 37.0 88-89 30.644972956667296 34.0 30.5 35.0 20.0 36.0 90-91 30.81145524869654 35.0 32.0 35.0 20.0 36.0 92-93 30.671145701126605 35.0 32.0 35.0 18.5 36.0 94-95 30.475404755251077 35.0 32.0 35.0 14.5 36.0 96-97 30.290764095409635 35.0 32.0 35.0 4.5 35.0 98-99 30.10834090946703 35.0 32.0 35.0 2.0 35.0 100 29.903139539937065 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23902501173176915 1101 2 0.2639235923303964 1101 3 0.2872894277161002 1101 4 0.3038353509387619 1101 5 0.26430269589032207 1101 6 0.25608227919435933 1101 7 -12.064585493707646 1101 8 -6.493149441092164 1101 9 -2.2441188677077335 1101 10-11 -0.19274158424692445 1101 12-13 0.2634397049392945 1101 14-15 0.4339966194921985 1101 16-17 0.5170608716749072 1101 18-19 0.5432121860176125 1101 20-21 0.4720916137594813 1101 22-23 0.49215509152821824 1101 24-25 0.546211069207807 1101 26-27 0.49731115251161384 1101 28-29 0.4344956085958742 1101 30-31 0.47095194006030994 1101 32-33 0.503493851658817 1101 34-35 0.4646614173719854 1101 36-37 0.5575893385502155 1101 38-39 0.5524495452208313 1101 40-41 0.5555536843742033 1101 42-43 0.5282649330776295 1101 44-45 0.4310521113992749 1101 46-47 0.4645547356044375 1101 48-49 0.3941371583851705 1101 50-51 0.44558002427341137 1101 52-53 0.4430431795807479 1101 54-55 0.36160083753934913 1101 56-57 0.3445974421392535 1101 58-59 0.3287118575493295 1101 60-61 0.33500632566836686 1101 62-63 0.3561793356650682 1101 64-65 0.2680921495326274 1101 66-67 -10.256480762705987 1101 68-69 -8.183700157383967 1101 70-71 -6.696639333488342 1101 72-73 -1.6185712738728526 1101 74-75 -0.32989691709691726 1101 76-77 -0.05216578923555204 1101 78-79 3.0606939516968374 1101 80-81 0.9331401206894121 1101 82-83 0.2603447448996796 1101 84-85 -5.391599195886336 1101 86-87 -5.9314026483394535 1101 88-89 -1.41735251596457 1101 90-91 -0.18194851650086008 1101 92-93 0.20063908182018153 1101 94-95 0.2508499981715886 1101 96-97 0.3609193357681022 1101 98-99 0.3936716419871722 1101 100 0.41754583597885997 1102 1 0.31828615693142126 1102 2 0.32812868930759365 1102 3 0.2958745765480977 1102 4 0.3183761982950415 1102 5 0.3655653247502926 1102 6 0.3396189740872586 1102 7 -12.181206734658268 1102 8 -6.5890876762238015 1102 9 -2.1817126109188933 1102 10-11 -0.13840866568801857 1102 12-13 0.41905835570061356 1102 14-15 0.5891886199663006 1102 16-17 0.6049225985190958 1102 18-19 0.6401570956204594 1102 20-21 0.6311110893725171 1102 22-23 0.575880237394216 1102 24-25 0.6122712095203298 1102 26-27 0.551381772896633 1102 28-29 0.5646323415716736 1102 30-31 0.5323855351223443 1102 32-33 0.5537499432179303 1102 34-35 0.579596935512015 1102 36-37 0.6733208117959535 1102 38-39 0.6342219952997397 1102 40-41 0.5566133506461455 1102 42-43 0.5426766659641942 1102 44-45 0.5020729467146552 1102 46-47 0.5356252779720947 1102 48-49 0.4298442916449119 1102 50-51 0.4488595657526204 1102 52-53 0.4502333104274143 1102 54-55 0.4595613629188975 1102 56-57 0.5063728767516196 1102 58-59 0.44643876042542985 1102 60-61 0.3734012593971414 1102 62-63 0.35111727681171345 1102 64-65 0.2786088543181293 1102 66-67 -10.331163344755726 1102 68-69 -6.743680843063093 1102 70-71 -6.320216900928244 1102 72-73 -1.0850153618252385 1102 74-75 -0.10056027177068216 1102 76-77 0.060553918344027124 1102 78-79 3.2200862737845135 1102 80-81 1.0887921969775327 1102 82-83 0.47377028895150985 1102 84-85 -5.438379812556388 1102 86-87 -5.920240320741382 1102 88-89 -1.2698075291554538 1102 90-91 0.06370139157802157 1102 92-93 0.3925901608320643 1102 94-95 0.4653991601620717 1102 96-97 0.5732656656246142 1102 98-99 0.5482644603030842 1102 100 0.6432389291410523 1103 1 0.19296230442272133 1103 2 0.1699637187117844 1103 3 0.17030820394982982 1103 4 0.2182835097624789 1103 5 0.2095559344429745 1103 6 0.17451584766642725 1103 7 -12.13997942179532 1103 8 -6.652355056086492 1103 9 -2.3791724502093814 1103 10-11 -0.40050268838091796 1103 12-13 0.15863102366182602 1103 14-15 0.35923827167746936 1103 16-17 0.34247174940423974 1103 18-19 0.382346140859255 1103 20-21 0.3738320737663159 1103 22-23 0.3942748623447443 1103 24-25 0.45914612386664544 1103 26-27 0.46046067824526205 1103 28-29 0.4218409355170962 1103 30-31 0.4040049990897927 1103 32-33 0.42324186662870744 1103 34-35 0.4345464812798312 1103 36-37 0.5020284668623276 1103 38-39 0.5421546448781029 1103 40-41 0.5087374421262112 1103 42-43 0.4265314227814514 1103 44-45 0.3892927491449214 1103 46-47 0.4739844979879777 1103 48-49 0.3776896364603246 1103 50-51 0.38726076379127505 1103 52-53 0.3674265058463604 1103 54-55 0.3989665147581718 1103 56-57 0.39960388353946286 1103 58-59 0.37131436613919533 1103 60-61 0.3579471605483491 1103 62-63 0.2538033525992631 1103 64-65 0.2214311583140045 1103 66-67 -10.215496408512546 1103 68-69 -8.189142418233015 1103 70-71 -6.743416057861388 1103 72-73 -1.6205574437877175 1103 74-75 -0.40958765086013216 1103 76-77 -0.12753162436495558 1103 78-79 3.01471084333782 1103 80-81 0.9223307772674012 1103 82-83 0.2624694709832802 1103 84-85 -5.504093082483152 1103 86-87 -6.03580392137912 1103 88-89 -1.4957085503749177 1103 90-91 -0.17439025470019942 1103 92-93 0.17977798853182136 1103 94-95 0.2325934685277602 1103 96-97 0.34219736145525204 1103 98-99 0.3916650583029444 1103 100 0.4532414594633849 1104 1 0.202609591420007 1104 2 0.26597604680551257 1104 3 0.2226583768417818 1104 4 0.21700038472158667 1104 5 0.24195038310975292 1104 6 0.2567023226671381 1104 7 -12.233095335740263 1104 8 -6.6063704108379575 1104 9 -2.30568701222483 1104 10-11 -0.2698628371636218 1104 12-13 0.2613789067630421 1104 14-15 0.4557820013452627 1104 16-17 0.4199915006948416 1104 18-19 0.4594806140547121 1104 20-21 0.48920491135861965 1104 22-23 0.4792029792233947 1104 24-25 0.5933466181312639 1104 26-27 0.5262997536717862 1104 28-29 0.549031688775699 1104 30-31 0.47386897565500874 1104 32-33 0.5114567928832869 1104 34-35 0.45665166938056245 1104 36-37 0.5014624785092536 1104 38-39 0.4958973571593859 1104 40-41 0.5250722502002105 1104 42-43 0.4786581867239619 1104 44-45 0.43584849148977156 1104 46-47 0.4175987115351205 1104 48-49 0.3666948911676684 1104 50-51 0.3784978933654486 1104 52-53 0.37453313681054823 1104 54-55 0.36260208603982136 1104 56-57 0.3267493795405514 1104 58-59 0.29864787899159495 1104 60-61 0.2622589482393849 1104 62-63 0.20212307939474528 1104 64-65 0.07806473139755354 1104 66-67 -10.44549245741555 1104 68-69 -8.349742288710857 1104 70-71 -6.827998785108186 1104 72-73 -1.7405441140783182 1104 74-75 -0.4836059584484147 1104 76-77 -0.1986401042076551 1104 78-79 2.935040587967759 1104 80-81 0.8387278214106324 1104 82-83 0.15729753534840896 1104 84-85 -5.556078345143021 1104 86-87 -6.074158469838363 1104 88-89 -1.536755238751649 1104 90-91 -0.26487992228794965 1104 92-93 0.07710941258771342 1104 94-95 0.12723453107924243 1104 96-97 0.25960368857923655 1104 98-99 0.3338026101663374 1104 100 0.38784863989010177 1105 1 0.13135742802363026 1105 2 0.11372194417190329 1105 3 0.12270341719042577 1105 4 0.17232890819052926 1105 5 0.13888013267707322 1105 6 0.2075389054392005 1105 7 -12.28670940667881 1105 8 -6.673567066175032 1105 9 -2.4346502501254506 1105 10-11 -0.3648861914184707 1105 12-13 0.1706921457062549 1105 14-15 0.28553865835049663 1105 16-17 0.3300872114357105 1105 18-19 0.3352990555752413 1105 20-21 0.3581858734705108 1105 22-23 0.35244776100707753 1105 24-25 0.35648474186710644 1105 26-27 0.3178963923272491 1105 28-29 0.30987536123227954 1105 30-31 0.3511853215482077 1105 32-33 0.3694371711388129 1105 34-35 0.3689327753713485 1105 36-37 0.49103334407632815 1105 38-39 0.4215021698040502 1105 40-41 0.4060979607325521 1105 42-43 0.34255125759995053 1105 44-45 0.2923158476630121 1105 46-47 0.3306648958252225 1105 48-49 0.29908275946635854 1105 50-51 0.4204900947124912 1105 52-53 0.3554647005030205 1105 54-55 0.29336026524202197 1105 56-57 0.2782847132770243 1105 58-59 0.2966083435744409 1105 60-61 0.2579866835022244 1105 62-63 0.13423215627611995 1105 64-65 0.002784167559120476 1105 66-67 -10.447669824640354 1105 68-69 -8.471601662733416 1105 70-71 -6.896953056643106 1105 72-73 -1.8442318968837483 1105 74-75 -0.6279978094816343 1105 76-77 -0.34698893753751037 1105 78-79 2.843872875225099 1105 80-81 0.7437965287943307 1105 82-83 0.04983699778350825 1105 84-85 -5.649637888066888 1105 86-87 -6.145393534583864 1105 88-89 -1.6569270593409264 1105 90-91 -0.3795276275238919 1105 92-93 0.042199910325269485 1105 94-95 0.052359121858479796 1105 96-97 0.09747897268791661 1105 98-99 0.1333341696884105 1105 100 0.21361317895635068 1106 1 0.07360052736214584 1106 2 0.08254719513641362 1106 3 0.08590336034363943 1106 4 0.09589509658739104 1106 5 0.07638401043111998 1106 6 0.12744079499803007 1106 7 -12.383098684569202 1106 8 -6.829786343328106 1106 9 -2.627692897275878 1106 10-11 -0.4920607799351515 1106 12-13 0.0696158832307674 1106 14-15 0.22286883759070264 1106 16-17 0.27425356798566725 1106 18-19 0.2717819261290657 1106 20-21 0.2541733196808522 1106 22-23 0.3038280611586046 1106 24-25 0.3608265053185846 1106 26-27 0.3041345929200645 1106 28-29 0.31559524272438466 1106 30-31 0.28954908154783965 1106 32-33 0.29973302683152525 1106 34-35 0.3342758498300711 1106 36-37 0.37758386006784406 1106 38-39 0.37869816849652693 1106 40-41 0.3384489172742349 1106 42-43 0.35036873204345653 1106 44-45 0.3143386775164245 1106 46-47 0.3479791686582132 1106 48-49 0.3423384297529992 1106 50-51 0.3831725014651681 1106 52-53 0.346928279580176 1106 54-55 0.32558480544763313 1106 56-57 0.30348686626823707 1106 58-59 0.2666734189704201 1106 60-61 0.2731856453893826 1106 62-63 0.24730225761434355 1106 64-65 0.18472661553442293 1106 66-67 -10.492591530377165 1106 68-69 -8.434820644959593 1106 70-71 -6.90790826356379 1106 72-73 -1.6979674282762431 1106 74-75 -0.4760832744969292 1106 76-77 -0.17765472319504028 1106 78-79 2.975602262763143 1106 80-81 0.8542856156909586 1106 82-83 0.17276332412459539 1106 84-85 -5.652245292994927 1106 86-87 -6.16690521584 1106 88-89 -1.581707101319516 1106 90-91 -0.2839688106830671 1106 92-93 0.11804711643378596 1106 94-95 0.19412457204288103 1106 96-97 0.21481283167580756 1106 98-99 0.22737219200711323 1106 100 0.3109451163189476 1107 1 0.01847371917655849 1107 2 0.0755527550897952 1107 3 0.07496331449728899 1107 4 0.04812090777775069 1107 5 0.13248630342305034 1107 6 0.10800643977965052 1107 7 -12.310515779551405 1107 8 -6.78224043807624 1107 9 -2.6140389303562515 1107 10-11 -0.5352557477189706 1107 12-13 0.10276555028958967 1107 14-15 0.21852138588729275 1107 16-17 0.23603198861943753 1107 18-19 0.2744407554862036 1107 20-21 0.2261885047906773 1107 22-23 0.27954910556204027 1107 24-25 0.3113475053945862 1107 26-27 0.267252847719746 1107 28-29 0.24813119063626488 1107 30-31 0.3139647759029316 1107 32-33 0.3127985308971404 1107 34-35 0.2722337496267855 1107 36-37 0.3607158858306221 1107 38-39 0.35768909452944087 1107 40-41 0.4076989398145088 1107 42-43 0.39486263107822595 1107 44-45 0.35844712735167406 1107 46-47 0.37183320500882644 1107 48-49 0.3091468125375627 1107 50-51 0.334511782899213 1107 52-53 0.3321376584979703 1107 54-55 0.29676089290865804 1107 56-57 0.21640906684460504 1107 58-59 0.22148095682377544 1107 60-61 0.1925343861451907 1107 62-63 0.15163665698171513 1107 64-65 0.09260325497421462 1107 66-67 -10.394897464249507 1107 68-69 -8.352805438526865 1107 70-71 -6.801216792417769 1107 72-73 -1.7347315950815556 1107 74-75 -0.46004981953434765 1107 76-77 -0.16548520808652256 1107 78-79 2.9454028871581066 1107 80-81 0.8548970951774031 1107 82-83 0.18726666451545526 1107 84-85 -5.6065850918948925 1107 86-87 -6.130198091569209 1107 88-89 -1.5861497537163132 1107 90-91 -0.29908289333249627 1107 92-93 0.08193250031870392 1107 94-95 0.11133663737370014 1107 96-97 0.16519481703850047 1107 98-99 0.24586449853893555 1107 100 0.33129927484523236 1108 1 0.04779577032814686 1108 2 0.07078463598480056 1108 3 0.0828447089452844 1108 4 0.01009896845953051 1108 5 0.022556804051056645 1108 6 0.08695372207770191 1108 7 -12.328212173384589 1108 8 -6.792428624300765 1108 9 -2.7002616236030974 1108 10-11 -0.578836521302712 1108 12-13 -0.03482450099997436 1108 14-15 0.11859915550284228 1108 16-17 0.15668724345383822 1108 18-19 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10-11 -0.5390360989307155 2216 12-13 -0.913794854486639 2216 14-15 -1.1210212115725895 2216 16-17 -1.3448250477780164 2216 18-19 -1.722381668194707 2216 20-21 -1.3360085130633266 2216 22-23 -1.120005293594943 2216 24-25 -1.189383243325345 2216 26-27 -1.571855233256187 2216 28-29 -1.7514736316199304 2216 30-31 -1.6706934945306386 2216 32-33 -2.4478569660895673 2216 34-35 -1.6154373811179923 2216 36-37 -1.307495730002664 2216 38-39 -1.5594979293478417 2216 40-41 -1.581155998376687 2216 42-43 -1.2283426172539365 2216 44-45 -1.3294162441330641 2216 46-47 -1.5121126284923747 2216 48-49 -1.5830819370154359 2216 50-51 -2.013662408246404 2216 52-53 -1.7556024601085767 2216 54-55 -1.3500963253455467 2216 56-57 -1.4268371733480834 2216 58-59 -1.8078638929229172 2216 60-61 -1.9840639105342817 2216 62-63 -1.7630385690081098 2216 64-65 -1.486167146700332 2216 66-67 1.9403134841426244 2216 68-69 1.117961174617946 2216 70-71 0.761659624733042 2216 72-73 -0.994913101724002 2216 74-75 -1.8277087352643875 2216 76-77 -1.4807900183147673 2216 78-79 1.1233929217292804 2216 80-81 -0.3504585261701685 2216 82-83 -1.2209481502131148 2216 84-85 0.6239720088475416 2216 86-87 0.6288778880038883 2216 88-89 -0.6930964932959363 2216 90-91 -1.0316326827368059 2216 92-93 -1.0886779716952084 2216 94-95 -1.0895953931278193 2216 96-97 -1.0619383457566478 2216 98-99 -1.0618345354245662 2216 100 -2.260437484853707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99515.0 3 19360.0 4 11279.0 5 10572.0 6 9650.0 7 10777.0 8 11973.0 9 13785.0 10 15682.0 11 17628.0 12 20033.0 13 22618.0 14 25563.0 15 27935.0 16 29545.0 17 30419.0 18 29260.0 19 27437.0 20 26963.0 21 27190.0 22 29034.0 23 33440.0 24 39989.0 25 49829.0 26 62938.0 27 76026.0 28 94684.0 29 119529.0 30 148985.0 31 183707.0 32 244883.0 33 345972.0 34 530165.0 35 727574.0 36 813958.0 37 1371939.0 38 1556438.0 39 514935.0 40 1122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.19795785663764 20.410883249343023 14.206549952254175 23.184608941765163 2 15.608816473009835 20.31161175398772 40.27154788537826 23.808023887624184 3 18.404149383287965 24.96680339983776 30.38062429925203 26.248422917622243 4 12.839652085180036 17.0212201991237 38.6183216624391 31.520806053257168 5 13.168314207294781 37.42173402748504 35.347069349285924 14.062882415934256 6 30.775934611568484 37.71178971848561 17.857760839653512 13.654514830292392 7 27.093141293697755 32.31551291255851 22.481047838792907 18.110297954950823 8 24.54071603386825 36.42930278930026 20.781033673154987 18.248947503676504 9 24.372488254357886 17.489802324465906 21.903047644137487 36.234661777038724 10-11 23.576829395784447 27.22561064624275 29.01651581448673 20.181044143486073 12-13 24.04243029542145 24.65340954271278 28.987251778748817 22.316908383116953 14-15 21.54347673697525 27.095806147492624 26.07027997003901 25.290437145493115 16-17 21.164028959247037 30.254773534971953 26.76478694736284 21.81641055841817 18-19 21.062826722867968 29.269753997770014 28.645669844359727 21.02174943500229 20-21 22.19807621592741 28.637294275510605 28.126001115935228 21.038628392626755 22-23 21.866674255134374 28.504989114787076 28.161570586776246 21.4667660433023 24-25 21.69799757303597 28.51322821871093 28.172339202869196 21.61643500538391 26-27 21.465030451811355 28.426715950538433 28.270149690207624 21.838103907442587 28-29 21.52030769350827 28.549219618986292 28.323381991464053 21.60709069604139 30-31 21.874449617488782 28.915894623100076 27.858850742788498 21.350805016622644 32-33 21.433110478140772 28.663494489614965 28.343359350297952 21.560035681946307 34-35 21.55546159665327 28.353473390401597 28.337751532073902 21.753313480871235 36-37 21.979619985951917 28.663210011987495 27.830860051231575 21.526309950829017 38-39 21.80179321986419 28.76034551215373 28.006429747660526 21.431431520321556 40-41 21.62000050858876 28.51776246240917 28.17237956705642 21.689857461945653 42-43 21.543366719680552 28.47638819027063 28.17507619919165 21.805168890857164 44-45 21.74550301682544 28.618501958205222 28.062995437761256 21.572999587208077 46-47 21.665453374637064 28.79182687200667 27.937833432462018 21.60488632089425 48-49 21.534571953713495 28.573144062426646 28.079378661269843 21.81290532259001 50-51 21.57969017823608 28.460294050076385 28.113228122821376 21.846787648866155 52-53 21.61210928630894 28.502703566353006 28.038971719770934 21.846215427567124 54-55 21.702294470193902 28.788643467759123 27.874949679130456 21.634112382916523 56-57 21.532008041736084 28.590586454179405 27.91712738552353 21.96027811856098 58-59 21.413375226107423 28.302164011502214 28.2213882955335 22.063072466856866 60-61 21.721999136330307 28.61824487760385 27.843532222017338 21.81622376404851 62-63 21.52942494270686 28.633229382122117 27.917765633130827 21.9195800420402 64-65 21.539758895922407 28.39046764665023 28.07043745398023 21.99933600344713 66-67 21.496437241249662 28.420554093908496 27.900453597856373 22.182555066985465 68-69 21.979410071720263 28.554252524747213 27.75317333714373 21.713164066388796 70-71 21.751604791959654 28.32081065473144 27.948063512464017 21.979521040844897 72-73 21.55479382844967 28.464648511423146 28.125732871937682 21.854824788189497 74-75 21.557481194099818 28.457003254392525 28.004843578047634 21.980671973460023 76-77 21.600037890719452 28.48464436882148 27.835315038592423 22.080002701866643 78-79 21.795775406763642 28.500451989239888 27.918175757344265 21.785596846652204 80-81 21.572278211164235 28.33180177512743 28.069435392900687 22.026484620807647 82-83 21.492650117441634 28.348331224095514 28.190392357255078 21.96862630120777 84-85 21.667665412698764 28.224670003121233 27.982985367358786 22.124679216821217 86-87 21.718159283724113 28.352019849160108 27.958070802110186 21.971750065005597 88-89 21.50484755001475 28.221572916150894 28.05883527479317 22.21474425904119 90-91 21.497500803777996 28.10293794228393 28.057957321423537 22.341603932514538 92-93 21.63157993880566 28.26266876209288 27.909899812434237 22.195851486667223 94-95 21.555090226234146 28.05310809151626 28.069947406573924 22.32185427567567 96-97 21.513281088853784 28.036712055756986 28.092692128169812 22.35731472721942 98-99 21.54225495195717 28.05053478849986 28.034347832522194 22.372862427020777 100 21.67611741115892 28.00380146789514 27.92553041187772 22.39455070906822 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2776.0 1 2165.5 2 1815.5 3 2064.0 4 1900.5 5 1674.0 6 1528.0 7 1490.5 8 1525.5 9 1493.0 10 1401.5 11 1293.0 12 1292.0 13 1405.0 14 1519.0 15 1681.5 16 1985.0 17 2468.0 18 3356.0 19 4716.5 20 6833.5 21 9894.0 22 14344.0 23 20980.0 24 29507.5 25 39980.0 26 54343.0 27 72499.0 28 94389.5 29 118599.0 30 140834.5 31 161189.5 32 181077.0 33 199929.0 34 216524.5 35 231338.0 36 243170.0 37 252947.5 38 261368.5 39 265992.0 40 267516.0 41 265334.0 42 260073.0 43 254878.5 44 250038.5 45 244919.0 46 239886.5 47 234220.5 48 228992.5 49 225305.5 50 219940.0 51 212296.0 52 203072.0 53 194930.5 54 188016.5 55 178596.5 56 164623.0 57 148182.5 58 133614.5 59 120103.5 60 104736.5 61 88400.0 62 74132.0 63 61048.5 64 49167.0 65 39542.0 66 31167.5 67 23982.5 68 18374.0 69 13917.5 70 10423.0 71 7814.0 72 5974.0 73 4391.5 74 3159.0 75 2267.0 76 1568.5 77 1099.0 78 757.5 79 542.0 80 366.5 81 223.0 82 148.5 83 117.0 84 98.0 85 66.0 86 53.0 87 42.5 88 31.5 89 27.5 90 24.5 91 20.5 92 19.0 93 15.0 94 15.0 95 16.0 96 14.5 97 16.0 98 15.0 99 25.0 100 45.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.017692968733496933 2 0.002664036356830717 3 0.0012647445330408453 4 0.002246939755508736 5 0.0019105715286361707 6 0.004211330200444518 7 11.620674590515412 8 1.3454729074902611E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.29640862335114E-4 18-19 0.0 20-21 0.0 22-23 6.727364537451306E-6 24-25 0.0 26-27 1.9509357158608784E-4 28-29 0.0 30-31 0.0 32-33 1.3454729074902611E-5 34-35 4.709155176215914E-5 36-37 6.727364537451306E-6 38-39 2.6909458149805223E-5 40-41 0.0 42-43 2.0182093612353916E-5 44-45 2.0182093612353916E-4 46-47 9.754678579304393E-4 48-49 7.601921927319976E-4 50-51 3.430955914100166E-4 52-53 2.4891248788569826E-4 54-55 3.632776850223705E-4 56-57 0.0014127465528647742 58-59 3.0945876872276007E-4 60-61 0.001231107710353589 62-63 0.002132574558372064 64-65 8.543752962563159E-4 66-67 5.810518934100218 68-69 5.461738719656054 70-71 0.0028927667511040615 72-73 0.001318563449340456 74-75 0.00298694985462838 76-77 0.005812442960357928 78-79 5.42023088045998 80-81 0.004810065644277684 82-83 0.0045947899790792415 84-85 5.810909121243389 86-87 0.003747142047360377 88-89 0.0016549316762130212 90-91 0.0032493170715889802 92-93 0.003646231579298608 94-95 0.004285331210356482 96-97 0.00298694985462838 98-99 0.005664440940533999 100 0.006256449019829715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7432331.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.36156073340533 #Duplication Level Percentage of deduplicated Percentage of total 1 91.3587731090642 79.81245005496909 2 6.20051298104345 10.83372982743391 3 1.3178680814175303 3.453930373001218 4 0.4834185760628022 1.6892880516946727 5 0.24009494130575476 1.048753439833304 6 0.12454825290713953 0.6528437856351957 7 0.0760834218587867 0.4652736535665186 8 0.05626334701347925 0.39322030457461815 9 0.03817160857828398 0.30012581709931785 >10 0.10349464508920034 1.2973450803119413 >50 6.927362754936236E-4 0.040029620594307834 >100 6.227761572804342E-5 0.005675633139450615 >500 1.6021768125548405E-5 0.007334358146380765 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.6909458149805223E-5 0.0 0.0 0.0 6 0.0 2.6909458149805223E-5 0.0 0.0 0.0 7 0.0 2.6909458149805223E-5 0.0 1.3454729074902611E-5 0.0 8 0.0 2.6909458149805223E-5 0.0 4.036418722470783E-5 0.0 9 1.3454729074902611E-5 2.6909458149805223E-5 0.0 8.072837444941566E-5 0.0 10-11 2.0182093612353916E-5 2.6909458149805223E-5 0.0 8.072837444941566E-5 0.0 12-13 2.6909458149805223E-5 3.363682268725653E-5 0.0 8.745573898686697E-5 0.0 14-15 2.6909458149805223E-5 5.3818916299610445E-5 0.0 1.210925616741235E-4 0.0 16-17 2.6909458149805223E-5 6.727364537451306E-5 0.0 1.4800201982392874E-4 0.0 18-19 4.036418722470783E-5 6.727364537451306E-5 0.0 1.4800201982392874E-4 0.0 20-21 5.3818916299610445E-5 8.072837444941566E-5 0.0 2.0182093612353916E-4 0.0 22-23 5.3818916299610445E-5 9.418310352431828E-5 0.0 3.565503204849192E-4 0.0 24-25 5.3818916299610445E-5 1.143651971366722E-4 0.0 6.189175374455201E-4 0.0 26-27 5.3818916299610445E-5 1.3454729074902612E-4 0.0 0.0010831056905296602 0.0 28-29 5.3818916299610445E-5 1.3454729074902612E-4 0.0 0.0026976731795179737 0.0 30-31 6.054628083706175E-5 1.3454729074902612E-4 0.0 0.007548103011020366 0.0 32-33 6.727364537451306E-5 1.6145674889883133E-4 0.0 0.01977845174010684 6.727364537451306E-6 34-35 6.727364537451306E-5 1.6818411343628265E-4 0.0 0.038729437642107165 1.3454729074902611E-5 36-37 6.727364537451306E-5 1.8163884251118525E-4 0.0 0.0682760226905933 1.3454729074902611E-5 38-39 6.727364537451306E-5 2.0854830066099049E-4 0.0 0.10964931459591884 1.3454729074902611E-5 40-41 6.727364537451306E-5 2.1527566519844178E-4 0.0 0.15595376470719619 1.3454729074902611E-5 42-43 6.727364537451306E-5 2.1527566519844178E-4 0.0 0.20477424915548031 1.3454729074902611E-5 44-45 6.727364537451306E-5 2.1527566519844178E-4 0.0 0.26408942228218846 1.3454729074902611E-5 46-47 6.727364537451306E-5 2.1527566519844178E-4 0.0 0.3356618535961329 1.3454729074902611E-5 48-49 6.727364537451306E-5 2.1527566519844178E-4 0.0 0.4144460196942251 1.3454729074902611E-5 50-51 6.727364537451306E-5 2.287303942733444E-4 0.0 0.49772406530333485 1.3454729074902611E-5 52-53 6.727364537451306E-5 2.287303942733444E-4 0.0 0.58267722468227 1.3454729074902611E-5 54-55 6.727364537451306E-5 2.354577588107957E-4 0.0 0.6800490989973401 1.3454729074902611E-5 56-57 7.400100991196437E-5 2.5563985242314964E-4 0.0 0.786388550240833 1.3454729074902611E-5 58-59 8.072837444941566E-5 2.825493105729548E-4 0.0 0.9045614357057026 1.3454729074902611E-5 60-61 8.072837444941566E-5 2.825493105729548E-4 0.0 1.0284054894756438 1.3454729074902611E-5 62-63 9.418310352431828E-5 2.960040396478575E-4 0.0 1.1542610252422827 1.3454729074902611E-5 64-65 1.0091046806176958E-4 3.0945876872276007E-4 0.0 1.2969887912688496 1.3454729074902611E-5 66-67 1.0763783259922089E-4 3.0945876872276007E-4 0.0 1.457907351004685 1.3454729074902611E-5 68-69 1.0763783259922089E-4 3.0945876872276007E-4 0.0 1.6231381514090262 2.6909458149805223E-5 70-71 1.210925616741235E-4 3.1618613326021134E-4 0.0 1.8222479596239727 2.6909458149805223E-5 72-73 1.2781992621157482E-4 3.2291349779766266E-4 0.0 2.043268794137398 2.6909458149805223E-5 74-75 1.3454729074902612E-4 3.2291349779766266E-4 0.0 2.248668688194861 2.6909458149805223E-5 76-77 1.3454729074902612E-4 3.2291349779766266E-4 0.0 2.46331870849132 2.6909458149805223E-5 78-79 1.3454729074902612E-4 3.2291349779766266E-4 0.0 2.7188240136237205 2.6909458149805223E-5 80-81 1.3454729074902612E-4 3.363682268725653E-4 0.0 3.032386474714326 2.6909458149805223E-5 82-83 1.3454729074902612E-4 3.363682268725653E-4 0.0 3.3592691175890845 2.6909458149805223E-5 84-85 1.4800201982392874E-4 3.363682268725653E-4 0.0 3.6919036571433645 2.6909458149805223E-5 86-87 1.4800201982392874E-4 3.363682268725653E-4 0.0 4.080684781127213 2.6909458149805223E-5 88 1.4800201982392874E-4 3.363682268725653E-4 0.0 4.411146381935896 2.6909458149805223E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6630 0.0 41.85149 1 GTATCAA 10085 0.0 34.31595 1 ATCAACG 10235 0.0 31.905735 3 TCAACGC 10350 0.0 31.550987 4 CAACGCA 11075 0.0 29.39314 5 TATCAAC 12110 0.0 27.515205 2 AACGCAG 12020 0.0 27.167448 6 GTACATG 18060 0.0 24.661911 1 ACGCAGA 14300 0.0 22.513033 7 TACATGG 18790 0.0 22.445864 2 CGCAGAG 14735 0.0 21.673887 8 ACATGGG 19170 0.0 21.306725 3 GTGGTAT 4330 0.0 18.326056 1 CATGGGG 13805 0.0 17.907845 4 GCAGAGT 17390 0.0 17.324345 9 GAGTACA 13755 0.0 17.009079 1 GTACCGT 525 1.8189894E-12 16.573805 6 AGAGTAC 14830 0.0 16.166533 10-11 CAGAGTA 16560 0.0 15.78882 10-11 TCTGTCG 2100 0.0 15.724823 8 >>END_MODULE