FastQCFastQC Report
Wed 25 May 2016
SRR2049513_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049513_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7432331
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217440.29255962900468235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211840.2850249807227369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124790.1679015641257097No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136450.058.4249421
GTATCAA235200.039.1002731
ATCAACG290200.031.263933
TCAACGC294550.030.8022144
TATCAAC301050.030.3091052
CAACGCA305600.029.7270035
GTGGTAT55800.028.5231421
AACGCAG320650.028.3903666
TGGTATC58800.026.6502232
ACGCAGA365750.024.8251847
CGCAGAG367900.024.5523648
GCAGAGT396500.022.4850489
GTACATG188950.022.0801331
GAGTACT292950.021.71373212-13
CAGAGTA388450.021.1120310-11
TACATGG194700.020.9308032
AGAGTAC369050.019.80863410-11
ACATGGG198050.019.6976153
GTACTTT325500.019.28246714-15
AGTACTT307200.018.58766212-13
Produced by FastQC (version 0.11.5)