##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049513_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7432331
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.798414521635273	31.0	31.0	34.0	30.0	34.0
2	31.988987169704902	33.0	31.0	34.0	30.0	34.0
3	32.01312858644213	34.0	31.0	34.0	30.0	34.0
4	35.62222914991273	37.0	35.0	37.0	33.0	37.0
5	35.74194273640396	37.0	35.0	37.0	33.0	37.0
6	35.80854337623015	37.0	35.0	37.0	35.0	37.0
7	35.78869993814861	37.0	35.0	37.0	33.0	37.0
8	35.78699966403541	37.0	35.0	37.0	33.0	37.0
9	37.534316891968345	39.0	37.0	39.0	35.0	39.0
10-11	37.48941523191042	39.0	37.0	39.0	34.5	39.0
12-13	37.46879592687678	39.0	37.0	39.0	34.5	39.0
14-15	38.74442345207715	40.0	38.0	41.0	34.5	41.0
16-17	38.7525950472335	40.0	38.0	41.0	35.0	41.0
18-19	38.82234786098735	40.0	38.0	41.0	35.0	41.0
20-21	38.850532154717	40.0	38.0	41.0	35.0	41.0
22-23	38.824420763822275	40.0	38.0	41.0	35.0	41.0
24-25	38.78549455076745	40.0	38.0	41.0	35.0	41.0
26-27	38.695017754187745	40.0	38.0	41.0	34.5	41.0
28-29	38.60906948304644	40.0	38.0	41.0	34.0	41.0
30-31	38.495497509462375	40.0	38.0	41.0	34.0	41.0
32-33	38.35185852190921	40.0	38.0	41.0	34.0	41.0
34-35	38.250664226337605	40.0	38.0	41.0	34.0	41.0
36-37	38.11157730999871	40.0	38.0	41.0	33.5	41.0
38-39	38.02086942844714	40.0	38.0	41.0	33.0	41.0
40-41	37.90159850254247	40.0	37.5	41.0	33.0	41.0
42-43	37.76241276121852	40.0	37.0	41.0	33.0	41.0
44-45	37.58540907287364	40.0	37.0	41.0	33.0	41.0
46-47	37.3473702933844	40.0	37.0	41.0	32.5	41.0
48-49	37.12255784894403	40.0	37.0	41.0	31.5	41.0
50-51	36.91404587605154	40.0	36.5	41.0	31.0	41.0
52-53	36.81252025777646	40.0	36.0	41.0	31.0	41.0
54-55	36.68233236652135	40.0	36.0	41.0	31.0	41.0
56-57	36.5130628600906	39.0	35.5	41.0	31.0	41.0
58-59	36.34404724439749	39.0	35.0	41.0	30.5	41.0
60-61	36.15845708432523	39.0	35.0	41.0	30.0	41.0
62-63	35.95867090687969	39.0	35.0	41.0	30.0	41.0
64-65	35.68642421872761	38.0	35.0	40.0	29.5	41.0
66-67	35.43563042334901	37.5	35.0	40.0	29.0	41.0
68-69	35.128644902924805	37.0	35.0	40.0	29.0	41.0
70-71	34.83740693195715	37.0	34.0	39.5	29.0	41.0
72-73	34.523430872494785	36.0	34.0	39.0	29.0	41.0
74-75	34.184169004313716	36.0	34.0	39.0	28.0	40.5
76-77	33.89419658516285	35.0	34.0	38.5	28.0	40.0
78-79	33.568212583643	35.0	34.0	37.0	28.0	39.0
80-81	33.263811514853145	35.0	34.0	37.0	28.0	39.0
82-83	32.968556096331014	35.0	34.0	36.0	27.0	39.0
84-85	32.6933553551369	35.0	33.0	36.0	27.0	37.0
86-87	32.41935248847233	35.0	33.0	36.0	26.5	37.0
88-89	32.2105406769424	35.0	33.0	35.0	26.0	37.0
90-91	32.06070612301847	35.0	33.0	35.0	26.0	36.0
92-93	31.90196171295385	35.0	33.0	35.0	26.0	36.0
94-95	31.72263809294823	35.0	33.0	35.0	26.0	36.0
96-97	31.557188855017355	35.0	33.0	35.0	25.0	36.0
98-99	31.378696266353046	35.0	33.0	35.0	25.0	35.0
100	31.268093280560297	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.37542336603866744
1101	2	0.39801541476774815
1101	3	0.4666240448497234
1101	4	0.38162385707925495
1101	5	0.31341107654114353
1101	6	0.3019608175755053
1101	7	0.289672425746204
1101	8	0.33622416804512767
1101	9	0.3533394636658045
1101	10-11	0.3702980179935338
1101	12-13	0.30307346920702827
1101	14-15	0.44421276356616346
1101	16-17	0.4470466185530242
1101	18-19	0.43994972077324235
1101	20-21	0.4503088944343503
1101	22-23	0.4656148824899944
1101	24-25	0.49947814303958893
1101	26-27	0.47515292931476694
1101	28-29	0.4706014962083813
1101	30-31	0.4614807793258393
1101	32-33	0.4647041504619551
1101	34-35	0.5072881670276175
1101	36-37	0.49297002714501303
1101	38-39	0.4811225653858102
1101	40-41	0.5010868117085607
1101	42-43	0.4292947343135083
1101	44-45	0.4784211476516518
1101	46-47	0.4994786405773084
1101	48-49	0.5041454171123547
1101	50-51	0.5547188481764351
1101	52-53	0.5258392070122966
1101	54-55	0.45394014578829456
1101	56-57	0.48530864402984264
1101	58-59	0.48767624130803
1101	60-61	0.5523887761479429
1101	62-63	0.5129986857210369
1101	64-65	0.48421692752648227
1101	66-67	0.5215302986847803
1101	68-69	0.4648141340222267
1101	70-71	0.4278891118625623
1101	72-73	0.41961683750407275
1101	74-75	0.4235399905135253
1101	76-77	0.3915119623924852
1101	78-79	0.42286499210763395
1101	80-81	0.4265824580191051
1101	82-83	0.4172660362476037
1101	84-85	0.43655108753300453
1101	86-87	0.45363837069263724
1101	88-89	0.41944346069951877
1101	90-91	0.44870122891526876
1101	92-93	0.47935748600832895
1101	94-95	0.4025827605338925
1101	96-97	0.41919441840967053
1101	98-99	0.4887897313624876
1101	100	0.5477397077935251
1102	1	0.3083137073083435
1102	2	0.3898728628509005
1102	3	0.47227040815286614
1102	4	0.3905382331285807
1102	5	0.32572978239723227
1102	6	0.2893769279215519
1102	7	0.326826279665859
1102	8	0.34739106493014305
1102	9	0.43748835125684593
1102	10-11	0.37651147293057363
1102	12-13	0.35462004025583127
1102	14-15	0.46913720999360464
1102	16-17	0.46702845444462326
1102	18-19	0.43516781517891445
1102	20-21	0.40957261862029526
1102	22-23	0.4299626081994461
1102	24-25	0.4057373312173098
1102	26-27	0.40856195805511675
1102	28-29	0.44217111297889033
1102	30-31	0.531837462360798
1102	32-33	0.532553446460085
1102	34-35	0.5440269911458486
1102	36-37	0.5743412240978927
1102	38-39	0.5099398194977738
1102	40-41	0.5450893477912757
1102	42-43	0.5730275732805765
1102	44-45	0.5882529420145772
1102	46-47	0.6265016247053907
1102	48-49	0.6787960425968151
1102	50-51	0.6892921110452477
1102	52-53	0.6479572955723967
1102	54-55	0.6503696948751809
1102	56-57	0.6343391766750202
1102	58-59	0.6252484336332813
1102	60-61	0.646830872588545
1102	62-63	0.6262127479420059
1102	64-65	0.619619832523135
1102	66-67	0.616551327451667
1102	68-69	0.5936828164680605
1102	70-71	0.6037099198062705
1102	72-73	0.6372321424279619
1102	74-75	0.660605249812896
1102	76-77	0.6192191660449282
1102	78-79	0.5653726244913315
1102	80-81	0.5439150351018114
1102	82-83	0.5521035378609582
1102	84-85	0.5415812311098165
1102	86-87	0.528889182110369
1102	88-89	0.526331395606519
1102	90-91	0.5291753913454755
1102	92-93	0.5691996821970129
1102	94-95	0.567875903826117
1102	96-97	0.6463067660460844
1102	98-99	0.6826989929759009
1102	100	0.7312780368028982
1103	1	0.29859096227160364
1103	2	0.33183358115766026
1103	3	0.38722844582775195
1103	4	0.28467921056814305
1103	5	0.2817894281584543
1103	6	0.2669213490476139
1103	7	0.27810285603145246
1103	8	0.23769282588703788
1103	9	0.3019631777950096
1103	10-11	0.2970223078991978
1103	12-13	0.23177895409319405
1103	14-15	0.3949310099948562
1103	16-17	0.37979967965294037
1103	18-19	0.3652971115457575
1103	20-21	0.35632862492574446
1103	22-23	0.3536741880345744
1103	24-25	0.33421643270560253
1103	26-27	0.35399660026672564
1103	28-29	0.3333806883634338
1103	30-31	0.3839232956353129
1103	32-33	0.40489244989547046
1103	34-35	0.4016963291668887
1103	36-37	0.4202249588381335
1103	38-39	0.4658136463663638
1103	40-41	0.4892694071227979
1103	42-43	0.5096448949578516
1103	44-45	0.5157723738706181
1103	46-47	0.49090630120528544
1103	48-49	0.46779072433408686
1103	50-51	0.501272868919294
1103	52-53	0.4790983531229642
1103	54-55	0.4373141117752226
1103	56-57	0.47261438155863544
1103	58-59	0.42112337503601793
1103	60-61	0.3786480567472239
1103	62-63	0.3931390936559964
1103	64-65	0.4263243894703308
1103	66-67	0.4622155462351998
1103	68-69	0.45126930080759564
1103	70-71	0.4695188978611853
1103	72-73	0.4434187293772993
1103	74-75	0.44794454674168804
1103	76-77	0.4160242954559479
1103	78-79	0.3675973018399574
1103	80-81	0.3415192152944826
1103	82-83	0.38497797909025877
1103	84-85	0.44396229599490056
1103	86-87	0.42755584016978077
1103	88-89	0.4494775596953602
1103	90-91	0.4318534683795221
1103	92-93	0.3957240832279503
1103	94-95	0.435228266502687
1103	96-97	0.46860118899766334
1103	98-99	0.4901100065345574
1103	100	0.48594622222737627
1104	1	0.32686601902143053
1104	2	0.31644297621199513
1104	3	0.38861220220529447
1104	4	0.3069415701196476
1104	5	0.2527910941624114
1104	6	0.24394962078242344
1104	7	0.2134885955073571
1104	8	0.249136377210192
1104	9	0.2942884025713255
1104	10-11	0.30036197473196324
1104	12-13	0.3087871851258299
1104	14-15	0.4011112806827484
1104	16-17	0.3807718760098808
1104	18-19	0.3520950032873813
1104	20-21	0.3703204324813143
1104	22-23	0.3878342066240279
1104	24-25	0.34464487087303297
1104	26-27	0.3957509963864325
1104	28-29	0.3929602194999191
1104	30-31	0.40922471148701334
1104	32-33	0.3944490042704487
1104	34-35	0.3803323786067736
1104	36-37	0.4011580301071973
1104	38-39	0.381812544085804
1104	40-41	0.4306258833147183
1104	42-43	0.4536433212300395
1104	44-45	0.48260889576438615
1104	46-47	0.5093871086048267
1104	48-49	0.49239001934429893
1104	50-51	0.5062293984636028
1104	52-53	0.5055509391201412
1104	54-55	0.4845751878493587
1104	56-57	0.5173921386441833
1104	58-59	0.48788560623978583
1104	60-61	0.49471538142672955
1104	62-63	0.47972849162960784
1104	64-65	0.5385125589967217
1104	66-67	0.4743412531963429
1104	68-69	0.4947101694113911
1104	70-71	0.5394564185184549
1104	72-73	0.5198047758811271
1104	74-75	0.5698008996191177
1104	76-77	0.49513640594572905
1104	78-79	0.4707668785608661
1104	80-81	0.49957655066124573
1104	82-83	0.46334507710599127
1104	84-85	0.48285458791637836
1104	86-87	0.5261405253487155
1104	88-89	0.47371334539322874
1104	90-91	0.4936297423356706
1104	92-93	0.5059753763523709
1104	94-95	0.4883372261813683
1104	96-97	0.49528048071949016
1104	98-99	0.5741831093788434
1104	100	0.6255790470352345
1105	1	0.22751076327148922
1105	2	0.2262125614654522
1105	3	0.2745992322530455
1105	4	0.24502485306061317
1105	5	0.18208462795310254
1105	6	0.24968913743275323
1105	7	0.21085373511158423
1105	8	0.2351951381835633
1105	9	0.21432510211466393
1105	10-11	0.23317491132098667
1105	12-13	0.22213810365700937
1105	14-15	0.3120550428029816
1105	16-17	0.2664013982902489
1105	18-19	0.29975165403701
1105	20-21	0.2965802068625365
1105	22-23	0.28018339443810447
1105	24-25	0.23900940576887564
1105	26-27	0.2997600044561608
1105	28-29	0.3341777685542766
1105	30-31	0.3129378294885541
1105	32-33	0.34880092369827764
1105	34-35	0.2884350539200753
1105	36-37	0.32304993327044684
1105	38-39	0.3351648179978568
1105	40-41	0.35067269608448726
1105	42-43	0.3507982725405938
1105	44-45	0.397162766940653
1105	46-47	0.4209053436904213
1105	48-49	0.461039225925191
1105	50-51	0.5362557762603757
1105	52-53	0.5018591944321926
1105	54-55	0.4848598178303831
1105	56-57	0.4536433636999675
1105	58-59	0.45031841062704814
1105	60-61	0.43202390781495126
1105	62-63	0.41074565283653186
1105	64-65	0.4739510768356183
1105	66-67	0.49448023186472767
1105	68-69	0.4897939945047014
1105	70-71	0.4740884847444491
1105	72-73	0.4343034934823109
1105	74-75	0.48053926940871605
1105	76-77	0.44873921679712225
1105	78-79	0.4107837686916014
1105	80-81	0.37445467064372906
1105	82-83	0.4200622403981029
1105	84-85	0.4036728209167677
1105	86-87	0.4606064010290183
1105	88-89	0.3994061535338531
1105	90-91	0.37822907090084357
1105	92-93	0.3684542636889425
1105	94-95	0.3932295255328704
1105	96-97	0.4049590469350548
1105	98-99	0.45221228134539615
1105	100	0.4732367403855271
1106	1	0.16923721210919496
1106	2	0.18954094453620485
1106	3	0.3103045035491121
1106	4	0.25075025199835466
1106	5	0.1894119511769361
1106	6	0.19414055164576638
1106	7	0.20558499416718945
1106	8	0.18892928943754583
1106	9	0.1335574690733523
1106	10-11	0.19808636611007557
1106	12-13	0.18944501575126793
1106	14-15	0.27156558662604624
1106	16-17	0.2533596954778474
1106	18-19	0.25504313929110367
1106	20-21	0.19811590315050154
1106	22-23	0.20136303115332055
1106	24-25	0.2363788897375727
1106	26-27	0.2924053893040508
1106	28-29	0.26605295031914267
1106	30-31	0.2712039328763467
1106	32-33	0.2637144362614521
1106	34-35	0.26829859987389426
1106	36-37	0.27844707372291566
1106	38-39	0.26140943872149336
1106	40-41	0.28669794503474577
1106	42-43	0.30580336351099646
1106	44-45	0.2987445455562394
1106	46-47	0.2665960818792641
1106	48-49	0.27037957131542356
1106	50-51	0.31799751665980125
1106	52-53	0.32980108711933553
1106	54-55	0.26695360234550947
1106	56-57	0.3075374556005315
1106	58-59	0.3071008930884105
1106	60-61	0.2761388479884346
1106	62-63	0.32044286350198803
1106	64-65	0.3089233142925494
1106	66-67	0.3169046944593106
1106	68-69	0.2875348352030258
1106	70-71	0.29955500264627943
1106	72-73	0.3111611785722488
1106	74-75	0.28339562148379827
1106	76-77	0.28505220908537865
1106	78-79	0.25317202813924666
1106	80-81	0.23224313109424344
1106	82-83	0.26444880985992114
1106	84-85	0.27653980751860985
1106	86-87	0.30791696800360313
1106	88-89	0.2999305098080356
1106	90-91	0.26189326343091324
1106	92-93	0.27342987751176295
1106	94-95	0.2722310413228364
1106	96-97	0.2789752786073443
1106	98-99	0.3195888811093326
1106	100	0.35656906244619435
1107	1	0.18647670004870776
1107	2	0.18543004846543454
1107	3	0.27059622288157215
1107	4	0.24467026185104856
1107	5	0.2011696848183675
1107	6	0.2070487917725785
1107	7	0.1900872165425227
1107	8	0.2147216491072399
1107	9	0.16961081588074478
1107	10-11	0.18159951978282152
1107	12-13	0.16038405231374497
1107	14-15	0.2153061204310518
1107	16-17	0.21044566822560995
1107	18-19	0.19190810442876227
1107	20-21	0.22244350066441854
1107	22-23	0.24192601223609955
1107	24-25	0.23118554276624792
1107	26-27	0.2235528346680553
1107	28-29	0.21426006807208609
1107	30-31	0.21201148306977302
1107	32-33	0.2172362713784608
1107	34-35	0.16547178312588784
1107	36-37	0.2861659214443577
1107	38-39	0.3103176692605558
1107	40-41	0.2922737544875105
1107	42-43	0.2671515190515308
1107	44-45	0.2675821159410816
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2216	100	-1.5722712791450206
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	505.0
3	70.0
4	75.0
5	144.0
6	405.0
7	1115.0
8	2512.0
9	4146.0
10	6082.0
11	8581.0
12	12331.0
13	17698.0
14	20676.0
15	26641.0
16	33152.0
17	46569.0
18	47670.0
19	26434.0
20	19466.0
21	18751.0
22	19904.0
23	21757.0
24	25376.0
25	31590.0
26	40492.0
27	52577.0
28	69484.0
29	91066.0
30	118220.0
31	151177.0
32	194427.0
33	252604.0
34	344017.0
35	498513.0
36	812626.0
37	1492200.0
38	2084281.0
39	836435.0
40	2562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58283278027471	20.54050036996229	14.677863545843554	23.198803303919448
2	15.680410079662963	20.56058733809883	39.78687923757776	23.97212334466045
3	18.000478988419513	25.22665166912664	30.859784213026064	25.91308512942778
4	12.68778909346513	17.589572247081232	37.91583422252355	31.806804436930094
5	12.653321207703087	37.4058739878994	35.41884216915308	14.52196263524444
6	30.358766103584994	38.17763955633032	17.337365699182314	14.126228640902367
7	26.302380773945615	32.91820560736598	22.347067158338348	18.432346460350058
8	24.731070238933114	36.09852682825886	20.408832706724176	18.761570226083848
9	24.406720049685656	16.80935416449709	21.95370601343375	36.83021977238351
10-11	23.615558833426554	27.06406644160493	28.70324935743578	20.617125367532743
12-13	24.49218481956533	24.521347948759004	29.093783510689107	21.892683720986554
14-15	21.689228007906	26.616108272910378	26.937985799877023	24.756677919306597
16-17	20.57682172658887	29.83187239642583	27.672126012687002	21.919179864298293
18-19	20.386403673356313	28.03963521000343	30.753958616751596	20.820002499888663
20-21	21.490216191932248	27.343581710771492	30.599067775641313	20.567134321654944
22-23	21.115064708501276	27.24212632618219	30.697280570523567	20.945528394792966
24-25	21.023821463279823	27.227931587008168	30.7068065187086	21.04144043100341
26-27	20.813759505597908	27.19081671685505	30.773595793836417	21.22182798371063
28-29	20.867123823280973	27.248147886029635	30.84931242177743	21.03541586891196
30-31	21.21663630486962	27.63760641625422	30.374668768928753	20.77108850994741
32-33	20.841840927283844	27.364761295262635	30.78117287706729	21.012224900386226
34-35	20.96059096399232	27.12788894897173	30.717509755687683	21.194010331348267
36-37	21.37206348856099	27.450479533271594	30.164150385659628	21.01330659250779
38-39	21.22227871713464	27.5450191871164	30.26809489512779	20.964607200621177
40-41	21.0843879262105	27.390108163912508	30.296269097810637	21.229234812066363
42-43	21.069715544154317	27.386757936372856	30.158721402477905	21.384805116994922
44-45	21.319260404306537	27.554343314365305	29.928894447784955	21.197501833543207
46-47	21.361992623848426	27.80223324284131	29.490943016396876	21.344831116913387
48-49	21.40675650748063	27.73062717470468	29.278358297013412	21.58425802080128
50-51	21.494447704226303	27.716405526072506	29.12186634314322	21.667280426557966
52-53	21.477824386454262	27.82565792616072	29.03050200536009	21.666015682024923
54-55	21.568616898251705	28.13967112067533	28.816087442822447	21.475624538250518
56-57	21.33574917478783	27.88865296768941	28.922050161651843	21.85354769587092
58-59	21.221240884824955	27.586106217878882	29.247526091289828	21.94512680600634
60-61	21.528165255288013	27.828429600350148	28.93105137540295	21.71235376895889
62-63	21.322947000073057	27.835688426686055	29.033838778170672	21.807525795070216
64-65	21.284473202283376	27.598696828760723	29.211609386072823	21.905220582883082
66-67	21.22486875248156	27.663467894527304	29.018601297493348	22.093062055497796
68-69	21.60777834085823	27.81375908942693	28.82825245728779	21.75021011242705
70-71	21.556171273857423	27.588471234663796	28.905958305678258	21.949399185800527
72-73	21.36370137444094	27.714131676858848	29.049352080794034	21.87281486790618
74-75	21.413853877067638	27.74100077082143	28.84179270272005	22.003352649390884
76-77	21.4536529656712	27.763463441011975	28.675135701033767	22.107747892283054
78-79	21.50655992749508	27.84527798374748	28.751111866370714	21.89705022238673
80-81	21.385278321555816	27.687079584864012	28.828608704035076	22.0990333895451
82-83	21.24459941958771	27.686902700280115	28.940679625144455	22.127818254987723
84-85	21.343404915631446	27.58849141675741	28.77067773219465	22.29742593541649
86-87	21.387477319529857	27.73813005220906	28.71271669094909	22.16167593731199
88-89	21.185809046467483	27.59346619123904	28.77598286350849	22.44474189878499
90-91	21.073884397174666	27.48481061180435	28.790054581828812	22.65125040919217
92-93	21.161619409796824	27.665659619218204	28.643561713431986	22.52915925755299
94-95	21.047653658480144	27.47206313420863	28.763885013160134	22.71639819415109
96-97	20.954416653026932	27.49333469495201	28.750013202453793	22.802235449567267
98-99	20.97054778996919	27.453684265973983	28.723770753674305	22.85199719038253
100	21.04478810827873	27.438695320658056	28.608729265904607	22.907787305158603
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	841.0
1	692.5
2	1456.5
3	2355.0
4	1524.5
5	832.5
6	1772.0
7	4561.0
8	7612.5
9	8783.0
10	7049.5
11	4782.0
12	4422.5
13	4858.0
14	5558.5
15	6251.5
16	7019.5
17	7993.0
18	9158.5
19	10519.0
20	12321.5
21	14741.0
22	18431.5
23	24362.5
24	31829.5
25	40982.0
26	53484.0
27	69989.0
28	91276.5
29	114488.5
30	135467.0
31	155272.0
32	174762.5
33	192521.0
34	209872.5
35	225917.5
36	237612.0
37	247195.5
38	255946.0
39	261270.0
40	263729.5
41	262604.5
42	258208.5
43	253784.5
44	249035.0
45	244227.0
46	239040.5
47	232035.0
48	225668.0
49	223514.0
50	219441.5
51	210843.0
52	201439.5
53	194295.0
54	188355.5
55	178270.5
56	164583.5
57	149206.0
58	134771.0
59	122591.5
60	107747.5
61	90463.0
62	75769.0
63	62701.0
64	50442.5
65	40129.5
66	31633.0
67	24480.0
68	18535.5
69	13713.5
70	9991.5
71	7400.0
72	5625.5
73	4094.5
74	2966.0
75	2078.5
76	1312.0
77	842.5
78	589.0
79	388.5
80	214.5
81	95.0
82	43.0
83	23.5
84	13.0
85	8.0
86	6.0
87	5.0
88	3.0
89	1.5
90	1.5
91	1.0
92	0.0
93	0.5
94	0.5
95	0.5
96	0.5
97	0.5
98	0.5
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06117865310358218
2	2.5563985242314964E-4
3	1.3454729074902611E-5
4	0.11988163605738227
5	0.0018836620704863654
6	0.0018163884251118526
7	0.0
8	0.0
9	6.727364537451306E-5
10-11	0.0
12-13	1.3454729074902611E-5
14-15	1.3454729074902611E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.7491147797373395E-4
30-31	2.623672169606009E-4
32-33	6.727364537451306E-6
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.8163884251118525E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	6.727364537451306E-6
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.0763783259922089E-4
80-81	2.623672169606009E-4
82-83	3.7673241409727314E-4
84-85	0.0
86-87	6.727364537451306E-6
88-89	6.054628083706175E-5
90-91	5.3818916299610445E-5
92-93	5.785533502208123E-4
94-95	4.978249757713965E-4
96-97	6.727364537451306E-6
98-99	3.363682268725653E-5
100	9.149215770933776E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7432331.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.28454202462267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.82147269318263	43.6093526952996
2	14.776178524458475	17.224455963442537
3	4.662585531248589	8.15269987018368
4	1.9810674120329095	4.6186242732097025
5	1.0020200962226409	2.9201141203902474
6	0.6474793686006901	2.264282308156984
7	0.417449315774389	1.7031589531881643
8	0.3052043080168538	1.4230954653363352
9	0.2186460750665125	1.1469317715659653
>10	1.0898400628629075	11.11418685281126
>50	0.05497430635868535	2.1705259554884178
>100	0.021788210936922514	2.1216505082778476
>500	8.088709018320397E-4	0.30503081619018385
>1k	3.9280065530651197E-4	0.39726834354205776
>5k	2.310592016936096E-5	0.08244607443737871
>10k+	6.931776050808287E-5	0.7461760284795562
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21744	0.29255962900468235	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21184	0.2850249807227369	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12479	0.1679015641257097	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.6909458149805223E-5	0.0	0.0	0.0
6	0.0	2.6909458149805223E-5	0.0	0.0	0.0
7	0.0	2.6909458149805223E-5	0.0	0.0	0.0
8	0.0	2.6909458149805223E-5	0.0	0.0	0.0
9	0.0	2.6909458149805223E-5	0.0	6.727364537451306E-5	0.0
10-11	0.0	2.6909458149805223E-5	0.0	6.727364537451306E-5	0.0
12-13	0.0	4.036418722470783E-5	0.0	8.072837444941566E-5	0.0
14-15	1.3454729074902611E-5	6.727364537451306E-5	0.0	1.0763783259922089E-4	0.0
16-17	1.3454729074902611E-5	8.072837444941566E-5	0.0	1.0763783259922089E-4	0.0
18-19	1.3454729074902611E-5	8.072837444941566E-5	0.0	1.0763783259922089E-4	0.0
20-21	1.3454729074902611E-5	8.072837444941566E-5	0.0	1.6145674889883133E-4	0.0
22-23	1.3454729074902611E-5	8.745573898686697E-5	0.0	3.0945876872276007E-4	0.0
24-25	1.3454729074902611E-5	1.143651971366722E-4	0.0	5.650986211459097E-4	0.0
26-27	1.3454729074902611E-5	1.210925616741235E-4	0.0	0.0010225594096925986	0.0
28-29	1.3454729074902611E-5	1.210925616741235E-4	0.0	0.0026303995341434606	0.0
30-31	1.3454729074902611E-5	1.3454729074902612E-4	0.0	0.007453919907496047	0.0
32-33	3.363682268725653E-5	1.5472938436138003E-4	0.0	0.019771724375569386	0.0
34-35	4.036418722470783E-5	1.6145674889883133E-4	0.0	0.038796711287481675	0.0
36-37	4.036418722470783E-5	1.7491147797373395E-4	0.0	0.06865275510469057	0.0
38-39	4.709155176215914E-5	1.8836620704863657E-4	0.0	0.11030859632058906	0.0
40-41	5.3818916299610445E-5	2.0182093612353916E-4	0.0	0.15671395689992818	0.0
42-43	5.3818916299610445E-5	2.287303942733444E-4	0.0	0.20572280755526093	0.0
44-45	5.3818916299610445E-5	2.489124878856983E-4	0.0	0.2654214404606038	0.0
46-47	5.3818916299610445E-5	2.8927667511040615E-4	0.0	0.3373706041886455	0.0
48-49	5.3818916299610445E-5	3.0945876872276007E-4	0.0	0.41622204393211226	0.0
50-51	5.3818916299610445E-5	3.498229559474679E-4	0.0	0.4995404537284467	0.0
52-53	5.3818916299610445E-5	3.632776850223705E-4	0.0	0.5850788938221401	0.0
54-55	5.3818916299610445E-5	3.632776850223705E-4	0.0	0.686608279421355	0.0
56-57	6.054628083706175E-5	3.632776850223705E-4	0.0	0.8077479326472408	0.0
58-59	6.727364537451306E-5	3.632776850223705E-4	0.0	0.9436205142101448	0.0
60-61	6.727364537451306E-5	3.700050495598218E-4	0.0	1.0857629995219535	0.0
62-63	6.727364537451306E-5	3.9018714317217573E-4	0.0	1.2296411448844244	0.0
64-65	7.400100991196437E-5	3.96914507709627E-4	0.0	1.393634110213875	0.0
66-67	8.072837444941566E-5	4.1709660132198097E-4	0.0	1.585794550861634	0.0
68-69	1.0091046806176958E-4	4.1709660132198097E-4	0.0	1.8040706206437793	0.0
70-71	1.210925616741235E-4	4.1709660132198097E-4	0.0	2.039528379454575	0.0
72-73	1.210925616741235E-4	4.574607885466888E-4	0.0	2.292806926925079	0.0
74-75	1.210925616741235E-4	4.910976112339453E-4	0.0	2.554635954722684	0.0
76-77	1.210925616741235E-4	5.381891629961045E-4	0.0	2.839647480716346	0.0
78-79	1.210925616741235E-4	5.449165275335558E-4	0.0	3.1670346759314136	0.0
80-81	1.210925616741235E-4	5.852807147582636E-4	0.0	3.527029407059508	0.0
82-83	1.210925616741235E-4	5.92008079295715E-4	0.0	3.9017099749728583	0.0
84-85	1.210925616741235E-4	5.92008079295715E-4	0.0	4.283649369222118	0.0
86-87	1.3454729074902612E-4	5.92008079295715E-4	0.0	4.688159071494528	0.0
88	1.4800201982392874E-4	5.92008079295715E-4	0.0	5.022206357601673	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13645	0.0	58.424942	1
GTATCAA	23520	0.0	39.100273	1
ATCAACG	29020	0.0	31.26393	3
TCAACGC	29455	0.0	30.802214	4
TATCAAC	30105	0.0	30.309105	2
CAACGCA	30560	0.0	29.727003	5
GTGGTAT	5580	0.0	28.523142	1
AACGCAG	32065	0.0	28.390366	6
TGGTATC	5880	0.0	26.650223	2
ACGCAGA	36575	0.0	24.825184	7
CGCAGAG	36790	0.0	24.552364	8
GCAGAGT	39650	0.0	22.485048	9
GTACATG	18895	0.0	22.080133	1
GAGTACT	29295	0.0	21.713732	12-13
CAGAGTA	38845	0.0	21.11203	10-11
TACATGG	19470	0.0	20.930803	2
AGAGTAC	36905	0.0	19.808634	10-11
ACATGGG	19805	0.0	19.697615	3
GTACTTT	32550	0.0	19.282467	14-15
AGTACTT	30720	0.0	18.587662	12-13
>>END_MODULE